Kleintierpraxis 52, 708-716
© M. & H. Schaper GmbH. 2007
Publiziert: 11/2007
Zusammenfassung
Die analytische Qualität der Bestimmung von Kreatinin imHundeserum wurde mittels Jaffé-Methode vergleichend miteinem enzymatischen Verfahren geprüft.Material und Methoden: Den Proben von gepooltem Hundeserumwurde Kreatinin in aufsteigender Menge zugesetzt.Danach ermittelten wir den Gesamtkreatiningehaltin jeder Probe mit beiden Verfahren und bestimmten diejeweilige Wiederfindungsrate (WFR): WFR (%) = Istwert/Sollwert × 100.Ergebnisse: Bei Kreatininkonzentrationen von 87–884 µmol/l(0,98–10 mg/dl) erwiesen sich beide Methoden mit denWFRen von 99 ± 2600 µmol/l ( gt; 30 mg/dl) oder bei Verdünnungder Substratprobe sollte das enzymatische Verfahren zurAnwendung kommen.Summary
Recovery rate in the determination of creatinine in dog serumusing the Jaffé method and an enzymatic method.In routine veterinary diagnostic procedures, serum creatinine ismostly detected by the method of Jaffé. Because of non-creatininechromogens the accuracy of the method is limited. Therefore,we analyzed the analytical quality of the determination ofcreatinine in dog serum with the Jaffé method by comparing itto results obtained by enzymic analysis.Material and Methods: Incrementing amounts of creatinine wereadded to samples of pooled canine serum. Subsequently, the totalcreatinine content (both exogenously and endogenously) wasdetermined in every sample both using the Jaffé method and viaenzymic analysis each as double detection. The same procedurewas performed with diluted serum samples (1:10). To estimate theaccuracy of the detection methods, the recovery rate was evaluated:recovery rate (%) = actual value/true value × 100.Results: At creatinine concentrations of 87–884 µmol/l (0.98–10mg/dl), the analytical quality of both methods was found to beequally suitable (recovery rates of 99 ± 2600 µmol/l ( gt;30 mg/dl) or whenusing diluted samples, the enzymic method should be used.