Berliner und Münchener Tierärztliche Wochenschrift 119
© Schlütersche Verlagsgesellschaft mbH & Co. KG. 2006
Publiziert: 11/2006
Zusammenfassung
Acht Lactobacillus spp.-spezifische Oligonukleotidsonden wurden benutzt, um RNA-Extrakte aus dem Jejunum von Aufzuchtferkeln zu untersuchen, die mit einer Weizen/Roggen basierten Diät mit oder ohne Zusatz einer Xylanase gefüttert wurden. RNA Verdünnungen aus Probengemischen oder Lactobacillus spp. Kulturen wurden verwendet, um Hybridisierungen mit Probenextrakten zu quantifizieren. Die Xylanase Supplementierung der Diät führte zu einer signifikanten (pSummary
rRNA extracts from the jejunum of rearing piglets fed a wheat/ rye based diet with or without a xylanase feed additive were hybridized against eight Lactobacillus spp. specific digoxigenin labelled oligonucleotide probes. Known concentrati-ons of sample- or culture RNA extracts were used to calibrate and quantify hybri-dization signals from different sample extracts and hybridizations. The xylanase feed additive tended to increase total eubacterial rRNA in the termi-nal jejunum, probably a result of increased Lactobacillus spp. rRNA in the centra and terminal jejunum. However, individual animal variations were high. L reuten was the major Lactobacillus species followed by L acidophilus and L mucosae. Lamylophilus and L.crispatus were not detected in all RNA extracts.The results of this study show that the xylanase feed additive modifies Lactobacillus spp. popu-lations on the species level in piglets in addition to their well known effects on digesta viscosity and nutrient digestibility.The implications of these effects for the intestinal microbiota require further analysis.
