%0 Journal Article %K dairy cow %K Paratuberculosis %K real-time PCR %K MAP screening %K feces %K milk %K North Germany %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2016 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 304-309 %R 10.2376/0005-9366-15103 %T Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study %V 129 %1 {"oldId":96497,"title":"Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study","topline":"","teaserText":"Eignung der Real-Time PCR f\u00fcr den Nachweis von Mycobacterium avium subsp. paratuberculosis in Proben chronisch kranker Milchk\u00fche: Ergebnisse einer Fall-Kontroll-Studie","content":"

Summary<\/span>
Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany. The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and \ufeff5 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.10%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.<\/p>

Keywords<\/span>
dairy cow, paratuberculosis, real-time PCR, MAP screening, feces, milk, North Germany<\/p>

Zusammenfassung<\/span>
Die Paratuberkulose, die durch Mycobacterium avium subsp. paratuberculosis (MAP) hervorgerufen wird, ist eine f\u00fcr Milchviehbetriebe wirtschaftlich relevante Erkrankung. Im Rahmen einer Fall-Kontroll-Studie, welche die Assoziation verschiedener Faktoren mit chronischen Erkrankungen in Milchviehbetrieben untersuchte, wurde ein f\u00fcr den deutschen Markt zugelassener Real-Time-PCR-Test verwendet, um MAP in bovinen Kot- und Milchproben zu diagnostizieren. Ziel dieser Studie war es, die Eignung dieses Testes zur Detektion von MAP aus Kot und Milch chronisch erkrankter Milchk\u00fche zu beschreiben. Das Gesamtprobenvolumen umfasste 928 Kot-, 922 Gesamtgemelks- und 92 Tankmilchproben aus 58 Fall- und \ufeff5 Kontrollbetrieben. Die Ergebnisse der Real-Time-PCR zeigten positive Ergebnisse f\u00fcr 11 (18,96 %) der Fall- und 6 (10,10 %) der Kontrollbetriebe, wobei alle Tankmilchproben negativ waren. Eine moderate Korrelation (kappa = 0,27) der Ergebnisse von Kot- bzw. Anfangsgemelksproben konnte festgestellt werden. Diese Ergebnisse zeigen den diagnostischen Wert der Real-Time-PCR-Methode zur Erkennung MAP-positiver Tiere und dass Kotproben besser als Milchproben f\u00fcr die Untersuchung der Gr\u00fcnde f\u00fcr chronische Erkrankungen bei Milchk\u00fchen geeignet sind. <\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Milchkuh, Paratuberkulose, Real-Time-PCR, MAP-Screening, Kotprobe, Milchprobe, Norddeutschland<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jul 10, 2016 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/potential-role-of-real-time-pcr-for-detecting-mycobacterium-avium-subsp-paratuberculosis-in-chronically-diseased-milking-cows-a-case-control-study\/150\/3130\/96497"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/potential-role-of-real-time-pcr-for-detecting-mycobacterium-avium-subsp-paratuberculosis-in-chronically-diseased-milking-cows-a-case-control-study\/150\/3130\/96497\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 129, Heft 7\/8 (2016), Seiten 304\u2013309","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-15103","doiFirstPage":"304","doiLastPage":"309","doiTransmitted":true,"doiAuthor":"Alajmi A, Klein G, Greiner M, Grabowski N, Fohler S, Campe A, Scheu T, Hoedemaker M, Abdulmawjood A","pdf":{"path":"http:\/\/data\/BMW_2016_07_08_0304.pdf","title":"BMW_2016_07_08_0304.pdf","description":"Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study "},"authors":[{"firstName":"A","middleName":"","lastName":"Alajmi"},{"firstName":"G","middleName":"","lastName":"Klein"},{"firstName":"M","middleName":"","lastName":"Greiner"},{"firstName":"N","middleName":"","lastName":"Grabowski"},{"firstName":"S","middleName":"","lastName":"Fohler"},{"firstName":"A","middleName":"","lastName":"Campe"},{"firstName":"T","middleName":"","lastName":"Scheu"},{"firstName":"M","middleName":"","lastName":"Hoedemaker"},{"firstName":"A","middleName":"","lastName":"Abdulmawjood"}],"contentOptimised":"

Summary<\/strong>
Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany. The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and \ufeff5 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.10%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.<\/p>

Keywords:<\/strong>
dairy cow, paratuberculosis, real-time PCR, MAP screening, feces, milk, North Germany<\/p>

Zusammenfassung<\/strong>
Die Paratuberkulose, die durch Mycobacterium avium subsp. paratuberculosis (MAP) hervorgerufen wird, ist eine f\u00fcr Milchviehbetriebe wirtschaftlich relevante Erkrankung. Im Rahmen einer Fall-Kontroll-Studie, welche die Assoziation verschiedener Faktoren mit chronischen Erkrankungen in Milchviehbetrieben untersuchte, wurde ein f\u00fcr den deutschen Markt zugelassener Real-Time-PCR-Test verwendet, um MAP in bovinen Kot- und Milchproben zu diagnostizieren. Ziel dieser Studie war es, die Eignung dieses Testes zur Detektion von MAP aus Kot und Milch chronisch erkrankter Milchk\u00fche zu beschreiben. Das Gesamtprobenvolumen umfasste 928 Kot-, 922 Gesamtgemelks- und 92 Tankmilchproben aus 58 Fall- und \ufeff5 Kontrollbetrieben. Die Ergebnisse der Real-Time-PCR zeigten positive Ergebnisse f\u00fcr 11 (18,96 %) der Fall- und 6 (10,10 %) der Kontrollbetriebe, wobei alle Tankmilchproben negativ waren. Eine moderate Korrelation (kappa = 0,27) der Ergebnisse von Kot- bzw. Anfangsgemelksproben konnte festgestellt werden. Diese Ergebnisse zeigen den diagnostischen Wert der Real-Time-PCR-Methode zur Erkennung MAP-positiver Tiere und dass Kotproben besser als Milchproben f\u00fcr die Untersuchung der Gr\u00fcnde f\u00fcr chronische Erkrankungen bei Milchk\u00fchen geeignet sind. <\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Milchkuh, Paratuberkulose, Real-Time-PCR, MAP-Screening, Kotprobe, Milchprobe, Norddeutschland<\/p>","primaryLanguage":"englisch","summary":"Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany. The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and \ufeff5 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.10%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.<\/p>

","keywords":["dairy cow","paratuberculosis","real-time PCR","MAP screening","feces","milk","North Germany"],"zusammenfassung":"Die Paratuberkulose, die durch Mycobacterium avium subsp. paratuberculosis (MAP) hervorgerufen wird, ist eine f\u00fcr Milchviehbetriebe wirtschaftlich relevante Erkrankung. Im Rahmen einer Fall-Kontroll-Studie, welche die Assoziation verschiedener Faktoren mit chronischen Erkrankungen in Milchviehbetrieben untersuchte, wurde ein f\u00fcr den deutschen Markt zugelassener Real-Time-PCR-Test verwendet, um MAP in bovinen Kot- und Milchproben zu diagnostizieren. Ziel dieser Studie war es, die Eignung dieses Testes zur Detektion von MAP aus Kot und Milch chronisch erkrankter Milchk\u00fche zu beschreiben. Das Gesamtprobenvolumen umfasste 928 Kot-, 922 Gesamtgemelks- und 92 Tankmilchproben aus 58 Fall- und \ufeff5 Kontrollbetrieben. Die Ergebnisse der Real-Time-PCR zeigten positive Ergebnisse f\u00fcr 11 (18,96 %) der Fall- und 6 (10,10 %) der Kontrollbetriebe, wobei alle Tankmilchproben negativ waren. Eine moderate Korrelation (kappa = 0,27) der Ergebnisse von Kot- bzw. Anfangsgemelksproben konnte festgestellt werden. Diese Ergebnisse zeigen den diagnostischen Wert der Real-Time-PCR-Methode zur Erkennung MAP-positiver Tiere und dass Kotproben besser als Milchproben f\u00fcr die Untersuchung der Gr\u00fcnde f\u00fcr chronische Erkrankungen bei Milchk\u00fchen geeignet sind. <\/p>

","schluesselwoerter":["Milchkuh","Paratuberkulose","Real-Time-PCR","MAP-Screening","Kotprobe","Milchprobe","Norddeutschland"],"translatedTitle":"Eignung der Real-Time PCR f\u00fcr den Nachweis von Mycobacterium avium subsp. paratuberculosis in Proben chronisch kranker Milchk\u00fche: Ergebnisse einer Fall-Kontroll-Studie","abstractE":"Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany. The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and \ufeff5 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.10%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.","date":{"year":2016,"date":"07\/2016","accepted":"2016-07-10"},"volume":"129","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"304-309","redirects":["potential-role-of-real-time-pcr-for-detecting-mycobacterium-avium-subsp-paratuberculosis-in-chronically-diseased-milking-cows-a-case-control-study\/150\/3130\/96497"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %7 7/8 %8 07/2016