%0 Journal Article %K porcine circovirus associated disease %K quantitative PCR %K depletion of lymphocytes %K histiocytosis %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2015 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 200-203 %R 10.2376/0005-9366-128-200 %T Detection of porcine circovirus type 2 and its association with PMWS in wild boars and domestic pigs in Germany: a histopathological, immunohistochemical and molecular biological study %V 128 %1 {"oldId":87700,"title":"Detection of porcine circovirus type 2 and its association with PMWS in wild boars and domestic pigs in Germany: a histopathological, immunohistochemical and molecular biological study","topline":"","teaserText":"Nachweis von porzinem Circovirus Typ 2 und seine Assoziation mit PMWS in Wild- und Hausschweinen in Deutschland: eine histopathologische, immunhistologische und molekularbiologische Untersuchung","content":"

Summary<\/span>
Beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (PMWS). For the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in Germany by histopathology, immunohistochemistry (IHC) and quantitative PCR (qPCR). Whereas 102\/340 domestic pigs were immunoreactive for PCV2 antigen in at least one examined tissue, only 8\/356 wild boars reacted positively. Similar findings could be found in qPCR: all domestic pigs showed viral DNA in at least one tissue, while in the examined tissues of 170 wild boars PCV2-DNA was not detectable. The specimens were examined histologically for histiocytosis and depletion of lymphocytes, both typical for PMWS. Based on these findings, six wild boars and 69 domestic pigs were assumed to be affected by PMWS. <\/p>

Keywords<\/span>
porcine circovirus associated disease, quantitative PCR, depletion of lymphocytes, histiocytosis <\/p>

Zusammenfassung<\/span>
Neben Hausschweinen k\u00f6nnen auch Wildschweine an Postweaning Multisystemic Wasting Syndrome (PMWS) erkranken. Zum ersten Mal wurden deutschlandweit Wild- (n = 356) und Hausschweine (n = 340) mittels Histopathologie, Immunhistochemie (IHC) und quantitativer PCR (qPCR) untersucht. W\u00e4hrend 102 von 340 Hausschweinen in mindestens einem untersuchten Gewebe immunhistologisch PCV2 Antigen aufwiesen, zeigten lediglich acht von 356 Wildschweinen Antigen. \u00c4hnliche Befunde ergab die qPCR: bei allen 340 Hausschweinen konnte in mindestens einem Gewebe PCV2-DNA nachgewiesen werden, w\u00e4hrend bei 170 von 356 Wildschweinen in keinem der untersuchten Gewebe mittels qPCR virale DNA nachweisbar war. Die Proben wurden histologisch auf das Vorliegen einer Histiozytose und einer Lymphozytendepletion untersucht, die typisch f\u00fcr PMWS sind. Anhand der Befunde konnten sechs Wildschweine und 69 Hausschweine als erkrankt an PMWS eingestuft werden.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Porzine Circoviren assoziierte Krankheiten, quantitative PCR, Lymphozytendepletion, Histiozytose<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"May 21, 2015 9:50:34 AM","oldUrls":["http:\/\/vetline.de\/detection-of-porcine-circovirus-type-2-and-its-association-with-pmws-in-wild-boars-and-domestic-pigs-in-germany-a-histopathological-immunohistochemical-and-molecular-biological-study\/150\/3130\/87700"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/detection-of-porcine-circovirus-type-2-and-its-association-with-pmws-in-wild-boars-and-domestic-pigs-in-germany-a-histopathological-immunohistochemical-and-molecular-biological-study\/150\/3130\/87700\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 200\u2013203 (2015) ","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-200","doiFirstPage":"200","doiLastPage":"203","doiTransmitted":true,"doiAuthor":"Hohloch C, Reiner G, Bronnert B, Willems H, Reinacher M","pdf":{"path":"http:\/\/data\/BMW_2015_05_06_0200.pdf","title":"BMW_2015_05_06_0200.pdf","description":"Detection of porcine circovirus type 2 and its association with PMWS in wild boars and domestic pigs in Germany: a histopathological, immunohistochemical and molecular biological study"},"authors":[{"firstName":"C","middleName":"","lastName":"Hohloch"},{"firstName":"G","middleName":"","lastName":"Reiner"},{"firstName":"B","middleName":"","lastName":"Bronnert"},{"firstName":"H","middleName":"","lastName":"Willems"},{"firstName":"M","middleName":"","lastName":"Reinacher"}],"contentOptimised":"

Summary<\/strong>
Beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (PMWS). For the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in Germany by histopathology, immunohistochemistry (IHC) and quantitative PCR (qPCR). Whereas 102\/340 domestic pigs were immunoreactive for PCV2 antigen in at least one examined tissue, only 8\/356 wild boars reacted positively. Similar findings could be found in qPCR: all domestic pigs showed viral DNA in at least one tissue, while in the examined tissues of 170 wild boars PCV2-DNA was not detectable. The specimens were examined histologically for histiocytosis and depletion of lymphocytes, both typical for PMWS. Based on these findings, six wild boars and 69 domestic pigs were assumed to be affected by PMWS. <\/p>

Keywords:<\/strong>
porcine circovirus associated disease, quantitative PCR, depletion of lymphocytes, histiocytosis <\/p>

Zusammenfassung<\/strong>
Neben Hausschweinen k\u00f6nnen auch Wildschweine an Postweaning Multisystemic Wasting Syndrome (PMWS) erkranken. Zum ersten Mal wurden deutschlandweit Wild- (n = 356) und Hausschweine (n = 340) mittels Histopathologie, Immunhistochemie (IHC) und quantitativer PCR (qPCR) untersucht. W\u00e4hrend 102 von 340 Hausschweinen in mindestens einem untersuchten Gewebe immunhistologisch PCV2 Antigen aufwiesen, zeigten lediglich acht von 356 Wildschweinen Antigen. \u00c4hnliche Befunde ergab die qPCR: bei allen 340 Hausschweinen konnte in mindestens einem Gewebe PCV2-DNA nachgewiesen werden, w\u00e4hrend bei 170 von 356 Wildschweinen in keinem der untersuchten Gewebe mittels qPCR virale DNA nachweisbar war. Die Proben wurden histologisch auf das Vorliegen einer Histiozytose und einer Lymphozytendepletion untersucht, die typisch f\u00fcr PMWS sind. Anhand der Befunde konnten sechs Wildschweine und 69 Hausschweine als erkrankt an PMWS eingestuft werden.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Porzine Circoviren assoziierte Krankheiten, quantitative PCR, Lymphozytendepletion, Histiozytose<\/p>","primaryLanguage":"englisch","summary":"Beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (PMWS). For the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in Germany by histopathology, immunohistochemistry (IHC) and quantitative PCR (qPCR). Whereas 102\/340 domestic pigs were immunoreactive for PCV2 antigen in at least one examined tissue, only 8\/356 wild boars reacted positively. Similar findings could be found in qPCR: all domestic pigs showed viral DNA in at least one tissue, while in the examined tissues of 170 wild boars PCV2-DNA was not detectable. The specimens were examined histologically for histiocytosis and depletion of lymphocytes, both typical for PMWS. Based on these findings, six wild boars and 69 domestic pigs were assumed to be affected by PMWS. <\/p>

","keywords":["porcine circovirus associated disease","quantitative PCR","depletion of lymphocytes","histiocytosis"],"zusammenfassung":"Neben Hausschweinen k\u00f6nnen auch Wildschweine an Postweaning Multisystemic Wasting Syndrome (PMWS) erkranken. Zum ersten Mal wurden deutschlandweit Wild- (n = 356) und Hausschweine (n = 340) mittels Histopathologie, Immunhistochemie (IHC) und quantitativer PCR (qPCR) untersucht. W\u00e4hrend 102 von 340 Hausschweinen in mindestens einem untersuchten Gewebe immunhistologisch PCV2 Antigen aufwiesen, zeigten lediglich acht von 356 Wildschweinen Antigen. \u00c4hnliche Befunde ergab die qPCR: bei allen 340 Hausschweinen konnte in mindestens einem Gewebe PCV2-DNA nachgewiesen werden, w\u00e4hrend bei 170 von 356 Wildschweinen in keinem der untersuchten Gewebe mittels qPCR virale DNA nachweisbar war. Die Proben wurden histologisch auf das Vorliegen einer Histiozytose und einer Lymphozytendepletion untersucht, die typisch f\u00fcr PMWS sind. Anhand der Befunde konnten sechs Wildschweine und 69 Hausschweine als erkrankt an PMWS eingestuft werden.<\/p>

","schluesselwoerter":["Porzine Circoviren assoziierte Krankheiten","quantitative PCR","Lymphozytendepletion","Histiozytose"],"translatedTitle":"Nachweis von porzinem Circovirus Typ 2 und seine Assoziation mit PMWS in Wild- und Hausschweinen in Deutschland: eine histopathologische, immunhistologische und molekularbiologische Untersuchung","abstractE":"Beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (PMWS). For the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in Germany by histopathology, immunohistochemistry (IHC) and quantitative PCR (qPCR). Whereas 102\/340 domestic pigs were immunoreactive for PCV2 antigen in at least one examined tissue, only 8\/356 wild boars reacted positively. Similar findings could be found in qPCR: all domestic pigs showed viral DNA in at least one tissue, while in the examined tissues of 170 wild boars PCV2-DNA was not detectable. The specimens were examined histologically for histiocytosis and depletion of lymphocytes, both typical for PMWS. Based on these findings, six wild boars and 69 domestic pigs were assumed to be affected by PMWS. ","date":{"year":2015,"date":"05\/2015","accepted":"2015-05-21"},"volume":"128","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"200-203","redirects":["detection-of-porcine-circovirus-type-2-and-its-association-with-pmws-in-wild-boars-and-domestic-pigs-in-germany-a-histopathological-immunohistochemical-and-molecular-biological-study\/150\/3130\/87700"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %7 5/6 %8 05/2015