%0 Journal Article %K Influenza A Virus %K pandemic H1N1 %K molecular diagnostics %K real-timeRT-PCR %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2010 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 286-292 %R 10.2376/0005-9366-123-286 %T New real-time reverse transcriptase polymerase chain reactions facilitate detection and differentiation of novel A/H1N1 influenza virus in porcine and human samples [engl.] %V 123 %1 {"oldId":70975,"title":"New real-time reverse transcriptase polymerase chain reactions facilitate detection and differentiation of novel A\/H1N1 influenza virus in porcine and human samples [engl.]","teaserText":"Influenza A viruses are maintained as a quasispecies cloud in several naturalhost reservoirs of avian as well as mammalian species. Accidental host exposure,selection and further adaptation of individual influenza A viruses during sporadictrans-species ...","content":"

Summary<\/span>
Influenza A viruses are maintained as a quasispecies cloud in several naturalhost reservoirs of avian as well as mammalian species. Accidental host exposure,selection and further adaptation of individual influenza A viruses during sporadictrans-species transmission may eventually lead to the establishment of new,stably circulating lineages in a new, possibly mammalian, host species. Givena high transmissibility of such a virus and a susceptible, immunologically na\u00efvepopulation, pandemic spread of such viruses within a short time may ensue.In April 2009, a novel multi-reassortant influenza A virus of subtype H1N1 hasemerged and regionally spread in humans in Mexico and the United States causingflu-like symptoms. Until June 2009 increasing levels of a multiregional, globalspread of this virus prompted the WHO to raise the pandemic alert to the highestlevel. Data from experimental infections in pigs as well as experience from naturaloutbreaks in swine farms world-wide have shown that porcine populations arefully susceptible to the new virus and are able to sustain uninterrupted transmissionchains. A broad front incursion of the new human pandemic virus into theporcine population would have a significant negative impact on measures torestrict further spread of the virus in the human population. Therefore, sensitivetools for monitoring and detection of such an incursion in a timely manner aremandatory. We have developed two real-time RT PCRs which are specific for thehemagglutinin gene of the novel A\/H1N1 virus and which allow detection ofinfected pigs with high sensitivity. These PCRs may become useful tools in futuresurveillance programmes.

Keywords:<\/span>
Influenza A Virus, pandemic H1N1, molecular diagnostics, real-timeRT-PCR


Zusammenfassung<\/span>
Influenza A Viren perpetuieren in Quasispezieswolken in verschiedenen nat\u00fcrlichenWirtsreservoiren bei V\u00f6geln und S\u00e4ugern. Aufgrund zuf\u00e4lliger Exposition,Selektion und fortschreitender Anpassung einzelner Virusindividuen im Zugesporadischer \u00dcbertragungsereignisse zwischen verschiedenen Wirtsspeziesk\u00f6nnen schlie\u00dflich neue, stabil zirkulierende Viruslinien entstehen. Unter derVoraussetzung, dass solche neu in einer Spezies auftauchende Influenzaviren einehohe \u00dcbertragbarkeit besitzen und auf eine immunologisch naive Populationtreffen, kann es in kurzer Zeit zu einer pandemischen Ausbreitung solcher Erregerkommen. Im April 2009 wurde ein neues, durch verschiedene Reassortierungsvorg\u00e4ngehervorgegangenes Influenza A Virus des Subtyps H1N1 erstmals beigrippekranken Menschen in Mexiko und den U.S.A. nachgewiesen. Bis Juni 2009weitete sich das Infektionsgeschehen global aus, worauf die WHO mit dem Ausrufeneiner neuerlichen Influenzapandemie reagierte. Infektionsexperimente mitdem neuen H1N1 Virus in Schweinen sowie Erfahrungen aus spontanen Ausbr\u00fcchenin Schweinehaltungen zeigten, dass Schweine voll empf\u00e4nglich gegen\u00fcber dem neuen Virus sind, und dass das Virus stabile Infektionsketten in Schweinenetablieren kann. Ein Einbruch dieses Virus auf breiter Front in die Schweinepopulationw\u00fcrde die Bek\u00e4mpfungsma\u00dfnahmen dieser Infektion in der menschlichenPopulation erschweren. Daher sind empfindliche Untersuchungswerkzeugeerforderlich, die ein sensitives Monitoring der Schweinepopulationen hinsichtlichder Verbreitung des neuen A\/H1N1 Virus erlauben. Wir haben zu diesem Zweckzwei real-time RT-PCRs entwickelt, die spezifisch und mit hoher Sensitivit\u00e4t dasH\u00e4magglutinin-Gen des neuen A\/H1N1 Virus detektieren. Diese PCRs k\u00f6nnen alswertvolle Werkzeuge in k\u00fcnftigen H1 Surveillance-Programmen bei Schweineneingesetzt werden.

Schl\u00fcsselw\u00f6rter:<\/span>
Influenza A Virus, pandemisches H1N1, molekulare Diagnostik,real-time RT-PCR<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jul 9, 2010 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/influenza-a-virus-pandemic-h1n1-molecular-diagnostics-real-time-rt-pcr\/150\/3130\/70975"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/influenza-a-virus-pandemic-h1n1-molecular-diagnostics-real-time-rt-pcr\/150\/3130\/70975","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 123: 7-8, 286-292 (2010)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-123-286","doiFirstPage":"286","doiLastPage":"292","doiTransmitted":true,"doiAuthor":"Hoffmann B, Harder T, Lange E, Kalthoff D, Reimann I, Grund C, Oehme R, Vahlenkamp TW, Beer M","pdf":{"path":"http:\/\/data\/bmtw_2010_07_0286.pdf","title":"bmtw_2010_07_0286.pdf","description":"New real-time reverse transcriptase polymerase chain reactions facilitate detection and differentiation of novel A\/H1N1 influenza virus in porcine and human samples [engl.]

"},"authors":[{"firstName":"B","middleName":"","lastName":"Hoffmann"},{"firstName":"T","middleName":"","lastName":"Harder"},{"firstName":"E","middleName":"","lastName":"Lange"},{"firstName":"D","middleName":"","lastName":"Kalthoff"},{"firstName":"I","middleName":"","lastName":"Reimann"},{"firstName":"C","middleName":"","lastName":"Grund"},{"firstName":"R","middleName":"","lastName":"Oehme"},{"firstName":"T","middleName":"W","lastName":"Vahlenkamp"},{"firstName":"M","middleName":"","lastName":"Beer"}],"contentOptimised":"

Summary<\/strong>
Influenza A viruses are maintained as a quasispecies cloud in several naturalhost reservoirs of avian as well as mammalian species. Accidental host exposure,selection and further adaptation of individual influenza A viruses during sporadictrans-species transmission may eventually lead to the establishment of new,stably circulating lineages in a new, possibly mammalian, host species. Givena high transmissibility of such a virus and a susceptible, immunologically na\u00efvepopulation, pandemic spread of such viruses within a short time may ensue.In April 2009, a novel multi-reassortant influenza A virus of subtype H1N1 hasemerged and regionally spread in humans in Mexico and the United States causingflu-like symptoms. Until June 2009 increasing levels of a multiregional, globalspread of this virus prompted the WHO to raise the pandemic alert to the highestlevel. Data from experimental infections in pigs as well as experience from naturaloutbreaks in swine farms world-wide have shown that porcine populations arefully susceptible to the new virus and are able to sustain uninterrupted transmissionchains. A broad front incursion of the new human pandemic virus into theporcine population would have a significant negative impact on measures torestrict further spread of the virus in the human population. Therefore, sensitivetools for monitoring and detection of such an incursion in a timely manner aremandatory. We have developed two real-time RT PCRs which are specific for thehemagglutinin gene of the novel A\/H1N1 virus and which allow detection ofinfected pigs with high sensitivity. These PCRs may become useful tools in futuresurveillance programmes.

Keywords:<\/strong>
Influenza A Virus, pandemic H1N1, molecular diagnostics, real-timeRT-PCR


Zusammenfassung<\/strong>
Influenza A Viren perpetuieren in Quasispezieswolken in verschiedenen nat\u00fcrlichenWirtsreservoiren bei V\u00f6geln und S\u00e4ugern. Aufgrund zuf\u00e4lliger Exposition,Selektion und fortschreitender Anpassung einzelner Virusindividuen im Zugesporadischer \u00dcbertragungsereignisse zwischen verschiedenen Wirtsspeziesk\u00f6nnen schlie\u00dflich neue, stabil zirkulierende Viruslinien entstehen. Unter derVoraussetzung, dass solche neu in einer Spezies auftauchende Influenzaviren einehohe \u00dcbertragbarkeit besitzen und auf eine immunologisch naive Populationtreffen, kann es in kurzer Zeit zu einer pandemischen Ausbreitung solcher Erregerkommen. Im April 2009 wurde ein neues, durch verschiedene Reassortierungsvorg\u00e4ngehervorgegangenes Influenza A Virus des Subtyps H1N1 erstmals beigrippekranken Menschen in Mexiko und den U.S.A. nachgewiesen. Bis Juni 2009weitete sich das Infektionsgeschehen global aus, worauf die WHO mit dem Ausrufeneiner neuerlichen Influenzapandemie reagierte. Infektionsexperimente mitdem neuen H1N1 Virus in Schweinen sowie Erfahrungen aus spontanen Ausbr\u00fcchenin Schweinehaltungen zeigten, dass Schweine voll empf\u00e4nglich gegen\u00fcber dem neuen Virus sind, und dass das Virus stabile Infektionsketten in Schweinenetablieren kann. Ein Einbruch dieses Virus auf breiter Front in die Schweinepopulationw\u00fcrde die Bek\u00e4mpfungsma\u00dfnahmen dieser Infektion in der menschlichenPopulation erschweren. Daher sind empfindliche Untersuchungswerkzeugeerforderlich, die ein sensitives Monitoring der Schweinepopulationen hinsichtlichder Verbreitung des neuen A\/H1N1 Virus erlauben. Wir haben zu diesem Zweckzwei real-time RT-PCRs entwickelt, die spezifisch und mit hoher Sensitivit\u00e4t dasH\u00e4magglutinin-Gen des neuen A\/H1N1 Virus detektieren. Diese PCRs k\u00f6nnen alswertvolle Werkzeuge in k\u00fcnftigen H1 Surveillance-Programmen bei Schweineneingesetzt werden.

Schl\u00fcsselw\u00f6rter:<\/strong>
Influenza A Virus, pandemisches H1N1, molekulare Diagnostik,real-time RT-PCR<\/p>","primaryLanguage":"englisch","summary":"Influenza A viruses are maintained as a quasispecies cloud in several naturalhost reservoirs of avian as well as mammalian species. Accidental host exposure,selection and further adaptation of individual influenza A viruses during sporadictrans-species transmission may eventually lead to the establishment of new,stably circulating lineages in a new, possibly mammalian, host species. Givena high transmissibility of such a virus and a susceptible, immunologically na\u00efvepopulation, pandemic spread of such viruses within a short time may ensue.In April 2009, a novel multi-reassortant influenza A virus of subtype H1N1 hasemerged and regionally spread in humans in Mexico and the United States causingflu-like symptoms. Until June 2009 increasing levels of a multiregional, globalspread of this virus prompted the WHO to raise the pandemic alert to the highestlevel. Data from experimental infections in pigs as well as experience from naturaloutbreaks in swine farms world-wide have shown that porcine populations arefully susceptible to the new virus and are able to sustain uninterrupted transmissionchains. A broad front incursion of the new human pandemic virus into theporcine population would have a significant negative impact on measures torestrict further spread of the virus in the human population. Therefore, sensitivetools for monitoring and detection of such an incursion in a timely manner aremandatory. We have developed two real-time RT PCRs which are specific for thehemagglutinin gene of the novel A\/H1N1 virus and which allow detection ofinfected pigs with high sensitivity. These PCRs may become useful tools in futuresurveillance programmes.","keywords":["Influenza A Virus","pandemic H1N1","molecular diagnostics","real-timeRT-PCR"],"zusammenfassung":"Influenza A Viren perpetuieren in Quasispezieswolken in verschiedenen nat\u00fcrlichenWirtsreservoiren bei V\u00f6geln und S\u00e4ugern. Aufgrund zuf\u00e4lliger Exposition,Selektion und fortschreitender Anpassung einzelner Virusindividuen im Zugesporadischer \u00dcbertragungsereignisse zwischen verschiedenen Wirtsspeziesk\u00f6nnen schlie\u00dflich neue, stabil zirkulierende Viruslinien entstehen. Unter derVoraussetzung, dass solche neu in einer Spezies auftauchende Influenzaviren einehohe \u00dcbertragbarkeit besitzen und auf eine immunologisch naive Populationtreffen, kann es in kurzer Zeit zu einer pandemischen Ausbreitung solcher Erregerkommen. Im April 2009 wurde ein neues, durch verschiedene Reassortierungsvorg\u00e4ngehervorgegangenes Influenza A Virus des Subtyps H1N1 erstmals beigrippekranken Menschen in Mexiko und den U.S.A. nachgewiesen. Bis Juni 2009weitete sich das Infektionsgeschehen global aus, worauf die WHO mit dem Ausrufeneiner neuerlichen Influenzapandemie reagierte. Infektionsexperimente mitdem neuen H1N1 Virus in Schweinen sowie Erfahrungen aus spontanen Ausbr\u00fcchenin Schweinehaltungen zeigten, dass Schweine voll empf\u00e4nglich gegen\u00fcber dem neuen Virus sind, und dass das Virus stabile Infektionsketten in Schweinenetablieren kann. Ein Einbruch dieses Virus auf breiter Front in die Schweinepopulationw\u00fcrde die Bek\u00e4mpfungsma\u00dfnahmen dieser Infektion in der menschlichenPopulation erschweren. Daher sind empfindliche Untersuchungswerkzeugeerforderlich, die ein sensitives Monitoring der Schweinepopulationen hinsichtlichder Verbreitung des neuen A\/H1N1 Virus erlauben. Wir haben zu diesem Zweckzwei real-time RT-PCRs entwickelt, die spezifisch und mit hoher Sensitivit\u00e4t dasH\u00e4magglutinin-Gen des neuen A\/H1N1 Virus detektieren. Diese PCRs k\u00f6nnen alswertvolle Werkzeuge in k\u00fcnftigen H1 Surveillance-Programmen bei Schweineneingesetzt werden.","schluesselwoerter":["Influenza A Virus","pandemisches H1N1","molekulare Diagnostik","real-time RT-PCR"],"translatedTitle":"Influenza A viruses are maintained as a quasispecies cloud in several naturalhost reservoirs of avian as well as mammalian species. Accidental host exposure,selection and further adaptation of individual influenza A viruses during sporadictrans-species ..","abstractE":"Influenza A viruses are maintained as a quasispecies cloud in several naturalhost reservoirs of avian as well as mammalian species. Accidental host exposure,selection and further adaptation of individual influenza A viruses during sporadictrans-species transmission may eventually lead to the establishment of new,stably circulating lineages in a new, possibly mammalian, host species. Givena high transmissibility of such a virus and a susceptible, immunologically na\u00efvepopulation, pandemic spread of such viruses within a short time may ensue.In April 2009, a novel multi-reassortant influenza A virus of subtype H1N1 hasemerged and regionally spread in humans in Mexico and the United States causingflu-like symptoms. Until June 2009 increasing levels of a multiregional, globalspread of this virus prompted the WHO to raise the pandemic alert to the highestlevel. Data from experimental infections in pigs as well as experience from naturaloutbreaks in swine farms world-wide have shown that porcine populations arefully susceptible to the new virus and are able to sustain uninterrupted transmissionchains. A broad front incursion of the new human pandemic virus into theporcine population would have a significant negative impact on measures torestrict further spread of the virus in the human population. Therefore, sensitivetools for monitoring and detection of such an incursion in a timely manner aremandatory. We have developed two real-time RT PCRs which are specific for thehemagglutinin gene of the novel A\/H1N1 virus and which allow detection ofinfected pigs with high sensitivity. These PCRs may become useful tools in futuresurveillance programmes.","date":{"year":2010,"date":"07\/2010","accepted":"2010-07-09"},"volume":"123","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"286-292","redirects":["influenza-a-virus-pandemic-h1n1-molecular-diagnostics-real-time-rt-pcr\/150\/3130\/70975"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %8 07/2010