%0 Journal Article
%K Serumkreatinin
%K Jaffé-Methode
%K enzymatisches Verfahren
%K Wiederfindungsrate
%B Kleintierpraxis
%C Hannover
%D 2007
%G German
%I M. & H. Schaper GmbH
%P 708-716
%T Wiederfindungsrate bei der Bestimmung von Kreatinin mit der Jaffé-Methode und einem enzymatischen Verfahren im Hundeserum
%V 52
%1 {"oldId":70911,"title":"Wiederfindungsrate bei der Bestimmung von Kreatinin mit der Jaff\u00e9-Methode und einem enzymatischen Verfahren im Hundeserum","teaserText":"Die analytische Qualit\u00e4t der Bestimmung von Kreatinin imHundeserum wurde mittels Jaff\u00e9-Methode vergleichend miteinem enzymatischen Verfahren gepr\u00fcft.Material und Methoden: Den Proben von gepooltem Hundeserumwurde Kreatinin in aufsteigender ...","content":"<p><span class=\"bold\">Zusammenfassung<\/span><br \/>Die analytische Qualit\u00e4t der Bestimmung von Kreatinin imHundeserum wurde mittels Jaff\u00e9-Methode vergleichend miteinem enzymatischen Verfahren gepr\u00fcft.Material und Methoden: Den Proben von gepooltem Hundeserumwurde Kreatinin in aufsteigender Menge zugesetzt.Danach ermittelten wir den Gesamtkreatiningehaltin jeder Probe mit beiden Verfahren und bestimmten diejeweilige Wiederfindungsrate (WFR): WFR (%) = Istwert\/Sollwert \u00d7 100.Ergebnisse: Bei Kreatininkonzentrationen von 87\u2013884 \u00b5mol\/l(0,98\u201310 mg\/dl) erwiesen sich beide Methoden mit denWFRen von 99 \u00b1  2600 \u00b5mol\/l ( gt; 30 mg\/dl) oder bei Verd\u00fcnnungder Substratprobe sollte das enzymatische Verfahren zurAnwendung kommen.<br \/><br \/><span class=\"bold\">Schl\u00fcsselw\u00f6rter:<\/span><br \/>Serumkreatinin, Jaff\u00e9-Methode, enzymatisches Verfahren,Wiederfindungsrate<br \/><br \/><br \/><span class=\"bold\">Summary<\/span><br \/><span class=\"bold\">Recovery rate in the determination of creatinine in dog serumusing the Jaff\u00e9 method and an enzymatic method.<\/span><br \/>In routine veterinary diagnostic procedures, serum creatinine ismostly detected by the method of Jaff\u00e9. Because of non-creatininechromogens the accuracy of the method is limited. Therefore,we analyzed the analytical quality of the determination ofcreatinine in dog serum with the Jaff\u00e9 method by comparing itto results obtained by enzymic analysis.Material and Methods: Incrementing amounts of creatinine wereadded to samples of pooled canine serum. Subsequently, the totalcreatinine content (both exogenously and endogenously) wasdetermined in every sample both using the Jaff\u00e9 method and viaenzymic analysis each as double detection. The same procedurewas performed with diluted serum samples (1:10). To estimate theaccuracy of the detection methods, the recovery rate was evaluated:recovery rate (%) = actual value\/true value \u00d7 100.Results: At creatinine concentrations of 87\u2013884 \u00b5mol\/l (0.98\u201310mg\/dl), the analytical quality of both methods was found to beequally suitable (recovery rates of 99 \u00b1  2600 \u00b5mol\/l ( gt;30 mg\/dl) or whenusing diluted samples, the enzymic method should be used.<br \/><br \/><span class=\"bold\">Keywords:<\/span><br \/>serum creatinine, Jaff\u00e9 method, enzymic method,recovery rate<\/p>","categories":["Abostufe KTP","Fachartikel","Kleintierpraxis"],"fromDate":"Nov 15, 2007 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/serumkreatinin-jaffe-methode-enzymatisches-verfahren\/150\/3231\/70911"],"doiLanguage":"deutsch","doiProductFormat":"Online","doiPublisher":"M. & H. Schaper GmbH","doiSerialWorkTitle":"Kleintierpraxis","doiDocumentUri":"http:\/\/www.vetline.de\/serumkreatinin-jaffe-methode-enzymatisches-verfahren\/150\/3231\/70911","doiSource":"Kleintierpraxis 52: Ausgabe 11, Seite 708\u0096716 (2007)","doiFirstPage":"708","doiLastPage":"716","doiTransmitted":false,"doiAuthor":"MOHR S, WILLING A, RADTKE E, WOLF K, HARTMANN H","pdf":{"path":"http:\/\/data\/ktp_2007_11_0708.pdf","title":"ktp_2007_11_0708.pdf","description":"Wiederfindungsrate bei der Bestimmung von Kreatinin mit der Jaff\u00e9-Methode und einem enzymatischen Verfahren im Hundeserum"},"authors":[{"firstName":"M","middleName":"OHRS","lastName":""},{"firstName":"W","middleName":"ILLINGA","lastName":""},{"firstName":"R","middleName":"ADTKEE","lastName":""},{"firstName":"W","middleName":"OLFK","lastName":""},{"firstName":"H","middleName":"ARTMANNH","lastName":""}],"contentOptimised":"<p><strong>Zusammenfassung<\/strong><br>Die analytische Qualit\u00e4t der Bestimmung von Kreatinin imHundeserum wurde mittels Jaff\u00e9-Methode vergleichend miteinem enzymatischen Verfahren gepr\u00fcft.Material und Methoden: Den Proben von gepooltem Hundeserumwurde Kreatinin in aufsteigender Menge zugesetzt.Danach ermittelten wir den Gesamtkreatiningehaltin jeder Probe mit beiden Verfahren und bestimmten diejeweilige Wiederfindungsrate (WFR): WFR (%) = Istwert\/Sollwert \u00d7 100.Ergebnisse: Bei Kreatininkonzentrationen von 87\u2013884 \u00b5mol\/l(0,98\u201310 mg\/dl) erwiesen sich beide Methoden mit denWFRen von 99 \u00b1  2600 \u00b5mol\/l ( gt; 30 mg\/dl) oder bei Verd\u00fcnnungder Substratprobe sollte das enzymatische Verfahren zurAnwendung kommen.<br><br><strong>Schl\u00fcsselw\u00f6rter:<\/strong><br>Serumkreatinin, Jaff\u00e9-Methode, enzymatisches Verfahren,Wiederfindungsrate<br><br><br><strong>Summary<\/strong><br><strong>Recovery rate in the determination of creatinine in dog serumusing the Jaff\u00e9 method and an enzymatic method.<\/strong><br>In routine veterinary diagnostic procedures, serum creatinine ismostly detected by the method of Jaff\u00e9. Because of non-creatininechromogens the accuracy of the method is limited. Therefore,we analyzed the analytical quality of the determination ofcreatinine in dog serum with the Jaff\u00e9 method by comparing itto results obtained by enzymic analysis.Material and Methods: Incrementing amounts of creatinine wereadded to samples of pooled canine serum. Subsequently, the totalcreatinine content (both exogenously and endogenously) wasdetermined in every sample both using the Jaff\u00e9 method and viaenzymic analysis each as double detection. The same procedurewas performed with diluted serum samples (1:10). To estimate theaccuracy of the detection methods, the recovery rate was evaluated:recovery rate (%) = actual value\/true value \u00d7 100.Results: At creatinine concentrations of 87\u2013884 \u00b5mol\/l (0.98\u201310mg\/dl), the analytical quality of both methods was found to beequally suitable (recovery rates of 99 \u00b1  2600 \u00b5mol\/l ( gt;30 mg\/dl) or whenusing diluted samples, the enzymic method should be used.<br><br><strong>Keywords:<\/strong><br>serum creatinine, Jaff\u00e9 method, enzymic method,recovery rate<\/p>","primaryLanguage":"deutsch","zusammenfassung":"Die analytische Qualit\u00e4t der Bestimmung von Kreatinin imHundeserum wurde mittels Jaff\u00e9-Methode vergleichend miteinem enzymatischen Verfahren gepr\u00fcft.Material und Methoden: Den Proben von gepooltem Hundeserumwurde Kreatinin in aufsteigender Menge zugesetzt.Danach ermittelten wir den Gesamtkreatiningehaltin jeder Probe mit beiden Verfahren und bestimmten diejeweilige Wiederfindungsrate (WFR): WFR (%) = Istwert\/Sollwert \u00d7 100.Ergebnisse: Bei Kreatininkonzentrationen von 87\u2013884 \u00b5mol\/l(0,98\u201310 mg\/dl) erwiesen sich beide Methoden mit denWFRen von 99 \u00b1  2600 \u00b5mol\/l ( gt; 30 mg\/dl) oder bei Verd\u00fcnnungder Substratprobe sollte das enzymatische Verfahren zurAnwendung kommen.","schluesselwoerter":["Serumkreatinin","Jaff\u00e9-Methode","enzymatisches Verfahren","Wiederfindungsrate"],"summary":"<strong>Recovery rate in the determination of creatinine in dog serumusing the Jaff\u00e9 method and an enzymatic method.<\/strong><br>In routine veterinary diagnostic procedures, serum creatinine ismostly detected by the method of Jaff\u00e9. Because of non-creatininechromogens the accuracy of the method is limited. Therefore,we analyzed the analytical quality of the determination ofcreatinine in dog serum with the Jaff\u00e9 method by comparing itto results obtained by enzymic analysis.Material and Methods: Incrementing amounts of creatinine wereadded to samples of pooled canine serum. Subsequently, the totalcreatinine content (both exogenously and endogenously) wasdetermined in every sample both using the Jaff\u00e9 method and viaenzymic analysis each as double detection. The same procedurewas performed with diluted serum samples (1:10). To estimate theaccuracy of the detection methods, the recovery rate was evaluated:recovery rate (%) = actual value\/true value \u00d7 100.Results: At creatinine concentrations of 87\u2013884 \u00b5mol\/l (0.98\u201310mg\/dl), the analytical quality of both methods was found to beequally suitable (recovery rates of 99 \u00b1  2600 \u00b5mol\/l ( gt;30 mg\/dl) or whenusing diluted samples, the enzymic method should be used.","keywords":["serum creatinine","Jaff\u00e9 method","enzymic method","recovery rate"],"translatedTitle":"","abstractE":"Die analytische Qualit\u00e4t der Bestimmung von Kreatinin imHundeserum wurde mittels Jaff\u00e9-Methode vergleichend miteinem enzymatischen Verfahren gepr\u00fcft.Material und Methoden: Den Proben von gepooltem Hundeserumwurde Kreatinin in aufsteigender ...","date":{"year":2007,"date":"11\/2007","accepted":"2007-11-15"},"volume":"52","openAccess":false,"journal":"Kleintierpraxis","titleImageId":947,"pages":"708-716","redirects":["serumkreatinin-jaffe-methode-enzymatisches-verfahren\/150\/3231\/70911"],"tierartCategories":[],"artikelartCategories":["Abostufe KTP","Fachartikel","Kleintierpraxis"]}
%8 11/2007