%0 Journal Article %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2007 %G English %I M. & H. Schaper GmbH %P 349-353 %R 10.2377/0341-6593-114-349 %T Effects of Immunization with Purified specific Proteins on the Formation of secondary hydatid Cysts in Mice %V 114 %1 {"oldId":70782,"title":"Effects of Immunization with Purified specific Proteins on the Formation of secondary hydatid Cysts in Mice","teaserText":"Hydatid cysts, mice, purification, protein, Echinococcus granulosus, Finnen, M\u00e4use, Reinigung, Protein,","content":"

Summary<\/span>
In this study, immunoreactive proteins (8, 68 and 116 kDa) of Echinococcus granulosusmetacestode stages were partially purified using isoelectrical focusing by aRotofor cell device. Sixty Swiss albino mice were separated into three experimentaland three control groups (n = 10). The mice of the three experimental groupswere immunized with 8, 68 and 116 kDa purified proteins, respectively. 25 daysafter immunization, serum samples from each group were examined to assessimmunization status by Western blotting. Protoscolices were applied intraperitoneallyto control and immunized groups. By necropsy, mice were examined forcyst formation, size, number and fertility 2, 5, 8, and 10 months after experimentalinfection, respectively. Immunization with all three proteins was found to have asignificant inhibitory effect on the metacestode formation of in mice (p

Zusammenfassung<\/span>
In dieser Studie wurden immunreaktive Proteine von Finnen von Echinococcusgranulosus mit der Gr\u00f6\u00dfe von 8, 68 und 116 kDa mittels isoelektrischer Fokussierungmit einer Rotofor Zell-Vorrichtung teilweise gereinigt. Die M\u00e4use dreierexperimenteller Gruppen wurden mit den gereinigten 8, 68 bzw. 116 kDaProteinen immunisiert. Serumproben wurden nach 25 Tagen mittels Westernblotauf Antik\u00f6rper gegen die eingesetzten Proteine getestet. Nach diesem Nachweiswurden die M\u00e4use (Kontroll- und Immunisierungsgruppe) mit Protoscolecesdurch intraperitoneale Injektion infiziert. Es wurden M\u00e4use der beiden Gruppennach jeweils 2, 5, 8 und 10 Monaten pathologisch-anatomisch untersucht. DieErgebnisse zeigen, dass eine Immunisierung mit den drei eingesetzten Proteinenoffenbar die Entwicklung von Finnen inhibieren kann (p <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Sep 1, 2007 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/hydatid-cysts-mice-purification-protein-echinococcus-granulosus\/150\/3130\/70782"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"M. & H. Schaper GmbH","doiSerialWorkTitle":"Dtsch.tier\u00e4rztl.Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/hydatid-cysts-mice-purification-protein-echinococcus-granulosus\/150\/3130\/70782","doiSource":"Dtsch.tier\u00e4rztl.Wschr. 114: 9, 349-353 (2007)","doiissn":"0341-6593","doiNr":"10.2377\/0341-6593-114-349","doiFirstPage":"349","doiLastPage":"353","doiTransmitted":true,"doiAuthor":"BURGU A, SARIMEHMETO\u00d0LU O, GONENC B, OGE H, SAHIN G, AYPAK S","pdf":{"path":"http:\/\/data\/dtw_2007_09_0349.pdf","title":"dtw_2007_09_0349.pdf","description":"Effects of Immunization with Purified specific Proteins on the Formation of secondary hydatid Cysts in Mice

"},"authors":[{"firstName":"B","middleName":"URGUA","lastName":""},{"firstName":"S","middleName":"ARIMEHMETO\u00d0LUO","lastName":""},{"firstName":"G","middleName":"ONENCB","lastName":""},{"firstName":"O","middleName":"GEH","lastName":""},{"firstName":"S","middleName":"AHING","lastName":""},{"firstName":"A","middleName":"YPAKS","lastName":""}],"contentOptimised":"

Summary<\/strong>
In this study, immunoreactive proteins (8, 68 and 116 kDa) of Echinococcus granulosusmetacestode stages were partially purified using isoelectrical focusing by aRotofor cell device. Sixty Swiss albino mice were separated into three experimentaland three control groups (n = 10). The mice of the three experimental groupswere immunized with 8, 68 and 116 kDa purified proteins, respectively. 25 daysafter immunization, serum samples from each group were examined to assessimmunization status by Western blotting. Protoscolices were applied intraperitoneallyto control and immunized groups. By necropsy, mice were examined forcyst formation, size, number and fertility 2, 5, 8, and 10 months after experimentalinfection, respectively. Immunization with all three proteins was found to have asignificant inhibitory effect on the metacestode formation of in mice (p

Zusammenfassung<\/strong>
In dieser Studie wurden immunreaktive Proteine von Finnen von Echinococcusgranulosus mit der Gr\u00f6\u00dfe von 8, 68 und 116 kDa mittels isoelektrischer Fokussierungmit einer Rotofor Zell-Vorrichtung teilweise gereinigt. Die M\u00e4use dreierexperimenteller Gruppen wurden mit den gereinigten 8, 68 bzw. 116 kDaProteinen immunisiert. Serumproben wurden nach 25 Tagen mittels Westernblotauf Antik\u00f6rper gegen die eingesetzten Proteine getestet. Nach diesem Nachweiswurden die M\u00e4use (Kontroll- und Immunisierungsgruppe) mit Protoscolecesdurch intraperitoneale Injektion infiziert. Es wurden M\u00e4use der beiden Gruppennach jeweils 2, 5, 8 und 10 Monaten pathologisch-anatomisch untersucht. DieErgebnisse zeigen, dass eine Immunisierung mit den drei eingesetzten Proteinenoffenbar die Entwicklung von Finnen inhibieren kann (p <\/p>","primaryLanguage":"englisch","summary":"In this study, immunoreactive proteins (8, 68 and 116 kDa) of Echinococcus granulosusmetacestode stages were partially purified using isoelectrical focusing by aRotofor cell device. Sixty Swiss albino mice were separated into three experimentaland three control groups (n = 10). The mice of the three experimental groupswere immunized with 8, 68 and 116 kDa purified proteins, respectively. 25 daysafter immunization, serum samples from each group were examined to assessimmunization status by Western blotting. Protoscolices were applied intraperitoneallyto control and immunized groups. By necropsy, mice were examined forcyst formation, size, number and fertility 2, 5, 8, and 10 months after experimentalinfection, respectively. Immunization with all three proteins was found to have asignificant inhibitory effect on the metacestode formation of in mice (p","zusammenfassung":"In dieser Studie wurden immunreaktive Proteine von Finnen von Echinococcusgranulosus mit der Gr\u00f6\u00dfe von 8, 68 und 116 kDa mittels isoelektrischer Fokussierungmit einer Rotofor Zell-Vorrichtung teilweise gereinigt. Die M\u00e4use dreierexperimenteller Gruppen wurden mit den gereinigten 8, 68 bzw. 116 kDaProteinen immunisiert. Serumproben wurden nach 25 Tagen mittels Westernblotauf Antik\u00f6rper gegen die eingesetzten Proteine getestet. Nach diesem Nachweiswurden die M\u00e4use (Kontroll- und Immunisierungsgruppe) mit Protoscolecesdurch intraperitoneale Injektion infiziert. Es wurden M\u00e4use der beiden Gruppennach jeweils 2, 5, 8 und 10 Monaten pathologisch-anatomisch untersucht. DieErgebnisse zeigen, dass eine Immunisierung mit den drei eingesetzten Proteinenoffenbar die Entwicklung von Finnen inhibieren kann (p <\/p>","translatedTitle":"Hydatid cysts, mice, purification, protein, Echinococcus granulosus, Finnen, M\u00e4use, Reinigung, Protein,","abstractE":"In this study, immunoreactive proteins (8, 68 and 116 kDa) of Echinococcus granulosusmetacestode stages were partially purified using isoelectrical focusing by aRotofor cell device. Sixty Swiss albino mice were separated into three experimentaland three control groups (n = 10). The mice of the three experimental groupswere immunized with 8, 68 and 116 kDa purified proteins, respectively. 25 daysafter immunization, serum samples from each group were examined to assessimmunization status by Western blotting. Protoscolices were applied intraperitoneallyto control and immunized groups. By necropsy, mice were examined forcyst formation, size, number and fertility 2, 5, 8, and 10 months after experimentalinfection, respectively. Immunization with all three proteins was found to have asignificant inhibitory effect on the metacestode formation of in mice (p","date":{"year":2007,"date":"09\/2007","accepted":"2007-09-01"},"volume":"114","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"349-353","redirects":["hydatid-cysts-mice-purification-protein-echinococcus-granulosus\/150\/3130\/70782"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %8 09/2007