%0 Journal Article %K swine %K phylogenetic analysis %K genotype 3 %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2012 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 281-289 %R 10.2376/0005-9366-125-281 %T First detection of Hepatitis E virus in Austrian pigs by RT-qPCR %V 125 %1 {"oldId":70565,"title":"First detection of Hepatitis E virus in Austrian pigs by RT-qPCR","topline":"","teaserText":"","content":"

Summary<\/span>
Long known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous HEV genotype 3 infections have been reported in industrialised countries. Genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. This study reports the first detected emergence of HEV in Austrian pigs. Five Austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within genotype 3. In addition, a reverse transcription quantitative real-time PCR (RT-qPCR) method using a MGB-hydrolysis probe was developed offering the possibility to detect the HEV genotype 3 in faeces, liquid- and tissue-samples from domestic pigs. The method was adapted to the strains found in Austria. Sensitivity of the assay was tested with different pig organs (liver, mesenteric lymph nodes and kidney) as well as with serum, bile and faeces samples. Within the dynamic range of the assay, a quantitative determination of virus loads was performed. For specificity testing several common swine pathogens were used. Results demonstrated that the proposed method allows implementation of reliable high-throughput screening of Austrian swine samples in the future.

Keywords:<\/span>
swine, phylogenetic analysis, genotype 3


Zusammenfassung<\/span>
In L\u00e4ndern mit unzureichenden sanit\u00e4ren Bedingungen kann das Hepatitis E-Virus (HEV) Krankheitsausbr\u00fcche bei Menschen verursachen, aber auch in industrialisierten L\u00e4ndern mehren sich die Meldungen \u00fcber autochthone HEV Genotyp 3- Erkrankungen. Dieser Genotyp stellt eine potenzielle zoonotische Gefahr dar, wobei die Schweinepopulation als gr\u00f6\u00dftes Virus-Reservoir gilt. In dieser Studie wurde HEV erstmals in \u00f6sterreichischen Hausschweinen nachgewiesen. F\u00fcnf St\u00e4mme wurden teilweise sequenziert und durch phylogenetische Analyse dem Genotyp 3 zugeordnet. Zus\u00e4tzlich wurde eine Real-Time RT-PCR (RT-qPCR) entwickelt, mit der, adaptiert an die gefundenen \u00f6sterreichischen St\u00e4mme, HEV Genotyp 3 sowohl in festem und fl\u00fcssigem Probenmaterial von Schweinen als auch in Schweinekot detektiert werden kann. Die Sensitivit\u00e4t wurde mit verschiedenen Schweineorganen (Leber, mesenteriale Lymphknoten und Niere) und auch mit Serum, Gallenfl\u00fcssigkeit und Kotproben getestet. Im Rahmen des dynamischen Bereiches erfolgte die quantitative Bestimmung der Virusmenge. Um die Spezifit\u00e4t der Methode zu bestimmen wurden verschiedene Erreger von Schweinekrankheiten getestet. Auf Grund der Ergebnisse bietet sich diese Methode f\u00fcr das Screening der \u00f6sterreichischen Schweinepopulation auf das Vorkommen des Hepatitis E-Virus an.

Schl\u00fcsselw\u00f6rter:<\/span>
Hausschwein, phylogenetische Analyse, Genotyp 3 <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Feb 24, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/swine-phylogenetic-analysis-genotype-3\/150\/3130\/70565","http:\/\/vetline.de\/swine-phylogenetic-analysis-genotype-3\/150\/3216\/70565"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/swine-phylogenetic-analysis-genotype-3\/150\/3130\/70565","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 7-8, 281-289 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-281","doiFirstPage":"281","doiLastPage":"289","doiTransmitted":true,"doiAuthor":"Zwettler D, Fink M, Revilla-Fern\u00e1ndez S, Steinrigl A, Winter P, K\u00f6fer J","pdf":{"path":"http:\/\/data\/bmtw_2012_07_0281.pdf","title":"bmtw - First detection of Hepatitis E virus in Austrian pigs by RT-qPCR ","description":""},"authors":[{"firstName":"D","middleName":"","lastName":"Zwettler"},{"firstName":"M","middleName":"","lastName":"Fink"},{"firstName":"S","middleName":"","lastName":"Revilla-Fern\u00e1ndez"},{"firstName":"A","middleName":"","lastName":"Steinrigl"},{"firstName":"P","middleName":"","lastName":"Winter"},{"firstName":"J","middleName":"","lastName":"K\u00f6fer"}],"contentOptimised":"

Summary<\/strong>
Long known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous HEV genotype 3 infections have been reported in industrialised countries. Genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. This study reports the first detected emergence of HEV in Austrian pigs. Five Austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within genotype 3. In addition, a reverse transcription quantitative real-time PCR (RT-qPCR) method using a MGB-hydrolysis probe was developed offering the possibility to detect the HEV genotype 3 in faeces, liquid- and tissue-samples from domestic pigs. The method was adapted to the strains found in Austria. Sensitivity of the assay was tested with different pig organs (liver, mesenteric lymph nodes and kidney) as well as with serum, bile and faeces samples. Within the dynamic range of the assay, a quantitative determination of virus loads was performed. For specificity testing several common swine pathogens were used. Results demonstrated that the proposed method allows implementation of reliable high-throughput screening of Austrian swine samples in the future.

Keywords:<\/strong>
swine, phylogenetic analysis, genotype 3


Zusammenfassung<\/strong>
In L\u00e4ndern mit unzureichenden sanit\u00e4ren Bedingungen kann das Hepatitis E-Virus (HEV) Krankheitsausbr\u00fcche bei Menschen verursachen, aber auch in industrialisierten L\u00e4ndern mehren sich die Meldungen \u00fcber autochthone HEV Genotyp 3- Erkrankungen. Dieser Genotyp stellt eine potenzielle zoonotische Gefahr dar, wobei die Schweinepopulation als gr\u00f6\u00dftes Virus-Reservoir gilt. In dieser Studie wurde HEV erstmals in \u00f6sterreichischen Hausschweinen nachgewiesen. F\u00fcnf St\u00e4mme wurden teilweise sequenziert und durch phylogenetische Analyse dem Genotyp 3 zugeordnet. Zus\u00e4tzlich wurde eine Real-Time RT-PCR (RT-qPCR) entwickelt, mit der, adaptiert an die gefundenen \u00f6sterreichischen St\u00e4mme, HEV Genotyp 3 sowohl in festem und fl\u00fcssigem Probenmaterial von Schweinen als auch in Schweinekot detektiert werden kann. Die Sensitivit\u00e4t wurde mit verschiedenen Schweineorganen (Leber, mesenteriale Lymphknoten und Niere) und auch mit Serum, Gallenfl\u00fcssigkeit und Kotproben getestet. Im Rahmen des dynamischen Bereiches erfolgte die quantitative Bestimmung der Virusmenge. Um die Spezifit\u00e4t der Methode zu bestimmen wurden verschiedene Erreger von Schweinekrankheiten getestet. Auf Grund der Ergebnisse bietet sich diese Methode f\u00fcr das Screening der \u00f6sterreichischen Schweinepopulation auf das Vorkommen des Hepatitis E-Virus an.

Schl\u00fcsselw\u00f6rter:<\/strong>
Hausschwein, phylogenetische Analyse, Genotyp 3 <\/p>","primaryLanguage":"englisch","summary":"Long known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous HEV genotype 3 infections have been reported in industrialised countries. Genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. This study reports the first detected emergence of HEV in Austrian pigs. Five Austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within genotype 3. In addition, a reverse transcription quantitative real-time PCR (RT-qPCR) method using a MGB-hydrolysis probe was developed offering the possibility to detect the HEV genotype 3 in faeces, liquid- and tissue-samples from domestic pigs. The method was adapted to the strains found in Austria. Sensitivity of the assay was tested with different pig organs (liver, mesenteric lymph nodes and kidney) as well as with serum, bile and faeces samples. Within the dynamic range of the assay, a quantitative determination of virus loads was performed. For specificity testing several common swine pathogens were used. Results demonstrated that the proposed method allows implementation of reliable high-throughput screening of Austrian swine samples in the future.","keywords":["swine","phylogenetic analysis","genotype 3"],"zusammenfassung":"In L\u00e4ndern mit unzureichenden sanit\u00e4ren Bedingungen kann das Hepatitis E-Virus (HEV) Krankheitsausbr\u00fcche bei Menschen verursachen, aber auch in industrialisierten L\u00e4ndern mehren sich die Meldungen \u00fcber autochthone HEV Genotyp 3- Erkrankungen. Dieser Genotyp stellt eine potenzielle zoonotische Gefahr dar, wobei die Schweinepopulation als gr\u00f6\u00dftes Virus-Reservoir gilt. In dieser Studie wurde HEV erstmals in \u00f6sterreichischen Hausschweinen nachgewiesen. F\u00fcnf St\u00e4mme wurden teilweise sequenziert und durch phylogenetische Analyse dem Genotyp 3 zugeordnet. Zus\u00e4tzlich wurde eine Real-Time RT-PCR (RT-qPCR) entwickelt, mit der, adaptiert an die gefundenen \u00f6sterreichischen St\u00e4mme, HEV Genotyp 3 sowohl in festem und fl\u00fcssigem Probenmaterial von Schweinen als auch in Schweinekot detektiert werden kann. Die Sensitivit\u00e4t wurde mit verschiedenen Schweineorganen (Leber, mesenteriale Lymphknoten und Niere) und auch mit Serum, Gallenfl\u00fcssigkeit und Kotproben getestet. Im Rahmen des dynamischen Bereiches erfolgte die quantitative Bestimmung der Virusmenge. Um die Spezifit\u00e4t der Methode zu bestimmen wurden verschiedene Erreger von Schweinekrankheiten getestet. Auf Grund der Ergebnisse bietet sich diese Methode f\u00fcr das Screening der \u00f6sterreichischen Schweinepopulation auf das Vorkommen des Hepatitis E-Virus an.","schluesselwoerter":["Hausschwein","phylogenetische Analyse","Genotyp 3"],"translatedTitle":"","abstractE":"Long known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous HEV genotype 3 infections have been reported in industrialised countries. Genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. This study reports the first detected emergence of HEV in Austrian pigs. Five Austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within genotype 3. In addition, a reverse transcription quantitative real-time PCR (RT-qPCR) method using a MGB-hydrolysis probe was developed offering the possibility to detect the HEV genotype 3 in faeces, liquid- and tissue-samples from domestic pigs. The method was adapted to the strains found in Austria. Sensitivity of the assay was tested with different pig organs (liver, mesenteric lymph nodes and kidney) as well as with serum, bile and faeces samples. Within the dynamic range of the assay, a quantitative determination of virus loads was performed. For specificity testing several common swine pathogens were used. Results demonstrated that the proposed method allows implementation of reliable high-throughput screening of Austrian swine samples in the future.","date":{"year":2012,"date":"02\/2012","accepted":"2012-02-24"},"volume":"125","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"281-289","redirects":["swine-phylogenetic-analysis-genotype-3\/150\/3130\/70565","swine-phylogenetic-analysis-genotype-3\/150\/3216\/70565"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} %8 02/2012