%0 Journal Article %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2007 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 412-422 %R 10.2376/0005-9366-120-412 %T Antimicrobial susceptibility of Pseudomonas aeruginosa from dogs and cats as well as Arconobocteriumpyogenes from cattle and swine as determined in the BfT-GermVet monitoring program 2004-2006 %V 120 %1 {"oldId":69698,"title":"Antimicrobial susceptibility of Pseudomonas aeruginosa from dogs and cats as well as Arconobocteriumpyogenes from cattle and swine as determined in the BfT-GermVet monitoring program 2004-2006","teaserText":"DOI 10.2376\/0005-9366-120-412","content":"

Summary<\/span>
During the BfT-GermVet monitoring program, Pseudomonas {P.) aeruginosa from dogs and cats (n = 99) as well as Arcanobacterium (A) pyogenes from cattle and swine (n = 90) were examined for their antimicrobial susceptibility. In general, P. aeruginosa is known to be resistant against many antimicrobial agents whereas A.pyogenes is thought to be susceptible to most agents in-vitro. However, repre-sentative and actual minimum inhibitory concentration (MIC) values are missing for both veterinary pathogens. In the present study, MIC values were determined and categorized according to the recommendations given in the Clinical and Laboratory Standards Institute (CLSI) documents M31-A2 and M31-S1. For susceptibility testing of Apyogenes, the CLSI methodology was slightly modified.Specific breakpoints were not available for most of the antimicrobial agents tested. P. aeruginosa isolates from infections of the skin,earand mouth as well as the urinary and genital tract of dogs and cats were either resistant orexhibited high MIC values to most antimicrobial agents tested. However, gentamicin resistant isolates were observed in only 27 % and 11 % (intermediate isolates 29 % and 39 %), respectively. For the same bacterium\/host animal\/organ System combina-tions,enrofloxacin resistance was detected in only 24 % and 11 % of the isolates (intermediate isolates 49 % and 61 %). For A.pyogenes, resistance was most preva-lent against tetracycline (33 %-56 %, bovine and porcine isolates) and sulfonami-des (26 %-40 %, bovine isolates).


Zusammenfassung<\/span>
Im Rahmen des Monitoring-Programmes BfT-GermVet wurden Pseudomonas (R) aeruginosa-\\so\\ate von Hunden und Katzen (N = 99) sowie Arcanobacterium (A) pyoge\/ies-lsolate von Rindern und Schweinen (N = 90) auf ihre Empfindlichkeit gegen\u00fcber antibakteriellen Wirkstoffen untersucht. Grunds\u00e4tzlich ist P.aeruginosa einer der am wenigsten empfindlichen Erreger in der Veterin\u00e4rmedizin, wohingegen A.pyogenes \u00fcberwiegend niedrige Resistenzraten aufweist. Unabh\u00e4ngig von dieser gegens\u00e4tzlichen generellen Resistenzsituation liegen f\u00fcr beide Erreger keine repr\u00e4sentativen und aktuellen Daten zur Empfindlichkeit vor. In der vorliegenden Studie wurden Minimale Hemmkonzentrationen (MHK-Werte) nach den Vorgaben der Dokumente M31-A2 und M31-S1 des Clinical and Laboratory Standards Institute (CLSI) bestimmt und bewertet. Die dort beschriebene Methode wurde f\u00fcr A.pyogenes adaptiert. Spezifische Grenzwerte f\u00fcr eine Beurteilung der ermittelten MHK-Werte liegen f\u00fcr die Mehrzahl der untersuchten Wirkstoffe und ndikationen nicht vor.P.aeruginosa-\\so\\aXe der Herkunft Haut\/Ohr\/Maul von Hunden und Katzen einerseits und dem Urogenitaltrakt der gleichen Tierarten andererseits waren gegen\u00fcber den meisten untersuchten Wirkstoffen resistent oder wiesen hohe MHK-Werte auf.Gentamicin-resistente Isolate wurden jedoch nur in 27 % bzw. 11 % der F\u00e4lle (intermedi\u00e4re Isolate zus\u00e4tzlich 29 % bzw. 39 %) eruiert.Gegen\u00fcber Enrofloxacin waren 24 % bzw. 11 % der Isolate resistent (intermedi\u00e4re Isolate 49 % bzw. 61 %). Bei den Apyoge\/ies-lsolaten waren vor allem gegen\u00fcberTetracyclin (33 %-56 %, bovine und porzine Isolate) und Sulfonamiden (26 %-40 %, nur bovine Isolate) Resistenzen zu beobachten.<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Sep 1, 2007 12:00:00 AM","toDate":"Jan 1, 2030 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/17083639\/150\/3130\/69698"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/17083639\/150\/3130\/69698","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 120: 9-10, 412-422 (2007)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-120-412","doiFirstPage":"412","doiLastPage":"422","doiTransmitted":true,"doiAuthor":"Werckenthin C, Alesik E, Grobbel M, L\u00fcbke-Becker A, Schwarz S, Wieler L H, Wallmann J","pdf":{"path":"http:\/\/data\/bmtw_2007_09_0412.pdf","title":"bmtw_2007_09_0412.pdf","description":"Antimicrobial susceptibility of Pseudomonas aeruginosa from dogs and cats as well as Arconobocteriumpyogenes from cattle and swine as determined in the BfT-GermVet monitoring program 2004-2006

"},"authors":[{"firstName":"C","middleName":"","lastName":"Werckenthin"},{"firstName":"E","middleName":"","lastName":"Alesik"},{"firstName":"M","middleName":"","lastName":"Grobbel"},{"firstName":"A","middleName":"","lastName":"L\u00fcbke-Becker"},{"firstName":"S","middleName":"","lastName":"Schwarz"},{"firstName":"L","middleName":"H","lastName":"Wieler"},{"firstName":"J","middleName":"","lastName":"Wallmann"}],"contentOptimised":"

Summary<\/strong>
During the BfT-GermVet monitoring program, Pseudomonas {P.) aeruginosa from dogs and cats (n = 99) as well as Arcanobacterium (A) pyogenes from cattle and swine (n = 90) were examined for their antimicrobial susceptibility. In general, P. aeruginosa is known to be resistant against many antimicrobial agents whereas A.pyogenes is thought to be susceptible to most agents in-vitro. However, repre-sentative and actual minimum inhibitory concentration (MIC) values are missing for both veterinary pathogens. In the present study, MIC values were determined and categorized according to the recommendations given in the Clinical and Laboratory Standards Institute (CLSI) documents M31-A2 and M31-S1. For susceptibility testing of Apyogenes, the CLSI methodology was slightly modified.Specific breakpoints were not available for most of the antimicrobial agents tested. P. aeruginosa isolates from infections of the skin,earand mouth as well as the urinary and genital tract of dogs and cats were either resistant orexhibited high MIC values to most antimicrobial agents tested. However, gentamicin resistant isolates were observed in only 27 % and 11 % (intermediate isolates 29 % and 39 %), respectively. For the same bacterium\/host animal\/organ System combina-tions,enrofloxacin resistance was detected in only 24 % and 11 % of the isolates (intermediate isolates 49 % and 61 %). For A.pyogenes, resistance was most preva-lent against tetracycline (33 %-56 %, bovine and porcine isolates) and sulfonami-des (26 %-40 %, bovine isolates).


Zusammenfassung<\/strong>
Im Rahmen des Monitoring-Programmes BfT-GermVet wurden Pseudomonas (R) aeruginosa-\\so\\ate von Hunden und Katzen (N = 99) sowie Arcanobacterium (A) pyoge\/ies-lsolate von Rindern und Schweinen (N = 90) auf ihre Empfindlichkeit gegen\u00fcber antibakteriellen Wirkstoffen untersucht. Grunds\u00e4tzlich ist P.aeruginosa einer der am wenigsten empfindlichen Erreger in der Veterin\u00e4rmedizin, wohingegen A.pyogenes \u00fcberwiegend niedrige Resistenzraten aufweist. Unabh\u00e4ngig von dieser gegens\u00e4tzlichen generellen Resistenzsituation liegen f\u00fcr beide Erreger keine repr\u00e4sentativen und aktuellen Daten zur Empfindlichkeit vor. In der vorliegenden Studie wurden Minimale Hemmkonzentrationen (MHK-Werte) nach den Vorgaben der Dokumente M31-A2 und M31-S1 des Clinical and Laboratory Standards Institute (CLSI) bestimmt und bewertet. Die dort beschriebene Methode wurde f\u00fcr A.pyogenes adaptiert. Spezifische Grenzwerte f\u00fcr eine Beurteilung der ermittelten MHK-Werte liegen f\u00fcr die Mehrzahl der untersuchten Wirkstoffe und ndikationen nicht vor.P.aeruginosa-\\so\\aXe der Herkunft Haut\/Ohr\/Maul von Hunden und Katzen einerseits und dem Urogenitaltrakt der gleichen Tierarten andererseits waren gegen\u00fcber den meisten untersuchten Wirkstoffen resistent oder wiesen hohe MHK-Werte auf.Gentamicin-resistente Isolate wurden jedoch nur in 27 % bzw. 11 % der F\u00e4lle (intermedi\u00e4re Isolate zus\u00e4tzlich 29 % bzw. 39 %) eruiert.Gegen\u00fcber Enrofloxacin waren 24 % bzw. 11 % der Isolate resistent (intermedi\u00e4re Isolate 49 % bzw. 61 %). Bei den Apyoge\/ies-lsolaten waren vor allem gegen\u00fcberTetracyclin (33 %-56 %, bovine und porzine Isolate) und Sulfonamiden (26 %-40 %, nur bovine Isolate) Resistenzen zu beobachten.<\/p>","primaryLanguage":"englisch","summary":"During the BfT-GermVet monitoring program, Pseudomonas {P.) aeruginosa from dogs and cats (n = 99) as well as Arcanobacterium (A) pyogenes from cattle and swine (n = 90) were examined for their antimicrobial susceptibility. In general, P. aeruginosa is known to be resistant against many antimicrobial agents whereas A.pyogenes is thought to be susceptible to most agents in-vitro. However, repre-sentative and actual minimum inhibitory concentration (MIC) values are missing for both veterinary pathogens. In the present study, MIC values were determined and categorized according to the recommendations given in the Clinical and Laboratory Standards Institute (CLSI) documents M31-A2 and M31-S1. For susceptibility testing of Apyogenes, the CLSI methodology was slightly modified.Specific breakpoints were not available for most of the antimicrobial agents tested. P. aeruginosa isolates from infections of the skin,earand mouth as well as the urinary and genital tract of dogs and cats were either resistant orexhibited high MIC values to most antimicrobial agents tested. However, gentamicin resistant isolates were observed in only 27 % and 11 % (intermediate isolates 29 % and 39 %), respectively. For the same bacterium\/host animal\/organ System combina-tions,enrofloxacin resistance was detected in only 24 % and 11 % of the isolates (intermediate isolates 49 % and 61 %). For A.pyogenes, resistance was most preva-lent against tetracycline (33 %-56 %, bovine and porcine isolates) and sulfonami-des (26 %-40 %, bovine isolates).","zusammenfassung":"Im Rahmen des Monitoring-Programmes BfT-GermVet wurden Pseudomonas (R) aeruginosa-\\so\\ate von Hunden und Katzen (N = 99) sowie Arcanobacterium (A) pyoge\/ies-lsolate von Rindern und Schweinen (N = 90) auf ihre Empfindlichkeit gegen\u00fcber antibakteriellen Wirkstoffen untersucht. Grunds\u00e4tzlich ist P.aeruginosa einer der am wenigsten empfindlichen Erreger in der Veterin\u00e4rmedizin, wohingegen A.pyogenes \u00fcberwiegend niedrige Resistenzraten aufweist. Unabh\u00e4ngig von dieser gegens\u00e4tzlichen generellen Resistenzsituation liegen f\u00fcr beide Erreger keine repr\u00e4sentativen und aktuellen Daten zur Empfindlichkeit vor. In der vorliegenden Studie wurden Minimale Hemmkonzentrationen (MHK-Werte) nach den Vorgaben der Dokumente M31-A2 und M31-S1 des Clinical and Laboratory Standards Institute (CLSI) bestimmt und bewertet. Die dort beschriebene Methode wurde f\u00fcr A.pyogenes adaptiert. Spezifische Grenzwerte f\u00fcr eine Beurteilung der ermittelten MHK-Werte liegen f\u00fcr die Mehrzahl der untersuchten Wirkstoffe und ndikationen nicht vor.P.aeruginosa-\\so\\aXe der Herkunft Haut\/Ohr\/Maul von Hunden und Katzen einerseits und dem Urogenitaltrakt der gleichen Tierarten andererseits waren gegen\u00fcber den meisten untersuchten Wirkstoffen resistent oder wiesen hohe MHK-Werte auf.Gentamicin-resistente Isolate wurden jedoch nur in 27 % bzw. 11 % der F\u00e4lle (intermedi\u00e4re Isolate zus\u00e4tzlich 29 % bzw. 39 %) eruiert.Gegen\u00fcber Enrofloxacin waren 24 % bzw. 11 % der Isolate resistent (intermedi\u00e4re Isolate 49 % bzw. 61 %). Bei den Apyoge\/ies-lsolaten waren vor allem gegen\u00fcberTetracyclin (33 %-56 %, bovine und porzine Isolate) und Sulfonamiden (26 %-40 %, nur bovine Isolate) Resistenzen zu beobachten.<\/p>","translatedTitle":"DOI 10.2376\/0005-9366-120-412","abstractE":"During the BfT-GermVet monitoring program, Pseudomonas {P.) aeruginosa from dogs and cats (n = 99) as well as Arcanobacterium (A) pyogenes from cattle and swine (n = 90) were examined for their antimicrobial susceptibility. In general, P. aeruginosa is known to be resistant against many antimicrobial agents whereas A.pyogenes is thought to be susceptible to most agents in-vitro. However, repre-sentative and actual minimum inhibitory concentration (MIC) values are missing for both veterinary pathogens. In the present study, MIC values were determined and categorized according to the recommendations given in the Clinical and Laboratory Standards Institute (CLSI) documents M31-A2 and M31-S1. For susceptibility testing of Apyogenes, the CLSI methodology was slightly modified.Specific breakpoints were not available for most of the antimicrobial agents tested. P. aeruginosa isolates from infections of the skin,earand mouth as well as the urinary and genital tract of dogs and cats were either resistant orexhibited high MIC values to most antimicrobial agents tested. However, gentamicin resistant isolates were observed in only 27 % and 11 % (intermediate isolates 29 % and 39 %), respectively. For the same bacterium\/host animal\/organ System combina-tions,enrofloxacin resistance was detected in only 24 % and 11 % of the isolates (intermediate isolates 49 % and 61 %). For A.pyogenes, resistance was most preva-lent against tetracycline (33 %-56 %, bovine and porcine isolates) and sulfonami-des (26 %-40 %, bovine isolates).","date":{"year":2007,"date":"09\/2007","accepted":"2007-09-01"},"volume":"120","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"412-422","redirects":["17083639\/150\/3130\/69698"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %8 09/2007