%0 Journal Article %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2006 %G German %I Schlütersche Verlagsgesellschaft mbH & Co. KG %T Einfluss eines Xylanase-Futterzusatzes auf Lactobacillus Spezies im Jejunum von Ferkeln %V 119 %1 {"oldId":69528,"title":"Einfluss eines Xylanase-Futterzusatzes auf Lactobacillus Spezies im Jejunum von Ferkeln","teaserText":"Acht Lactobacillus spp.-spezifische Oligonukleotidsonden wurden benutzt, um RNA-Extrakte aus dem Jejunum von Aufzuchtferkeln zu untersuchen, die mit einer Weizen\/Roggen basierten Di\u00e4t mit oder ohne Zusatz einer Xylanase gef\u00fcttert wurden. RNA Verd\u00fcnnungen ...","content":"

Zusammenfassung<\/span>
Acht Lactobacillus spp.-spezifische Oligonukleotidsonden wurden benutzt, um RNA-Extrakte aus dem Jejunum von Aufzuchtferkeln zu untersuchen, die mit einer Weizen\/Roggen basierten Di\u00e4t mit oder ohne Zusatz einer Xylanase gef\u00fcttert wurden. RNA Verd\u00fcnnungen aus Probengemischen oder Lactobacillus spp. Kulturen wurden verwendet, um Hybridisierungen mit Probenextrakten zu quantifizieren. Die Xylanase Supplementierung der Di\u00e4t f\u00fchrte zu einer signifikanten (p

Summary<\/span>
rRNA extracts from the jejunum of rearing piglets fed a wheat\/ rye based diet with or without a xylanase feed additive were hybridized against eight Lactobacillus spp. specific digoxigenin labelled oligonucleotide probes. Known concentrati-ons of sample- or culture RNA extracts were used to calibrate and quantify hybri-dization signals from different sample extracts and hybridizations. The xylanase feed additive tended to increase total eubacterial rRNA in the termi-nal jejunum, probably a result of increased Lactobacillus spp. rRNA in the centra and terminal jejunum. However, individual animal variations were high. L reuten was the major Lactobacillus species followed by L acidophilus and L mucosae. Lamylophilus and L.crispatus were not detected in all RNA extracts.The results of this study show that the xylanase feed additive modifies Lactobacillus spp. popu-lations on the species level in piglets in addition to their well known effects on digesta viscosity and nutrient digestibility.The implications of these effects for the intestinal microbiota require further analysis.<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Nov 1, 2006 12:00:00 AM","toDate":"Jan 1, 2030 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/11761744\/150\/3130\/69528"],"doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiDocumentUri":"http:\/\/www.vetline.de\/11761744\/150\/3130\/69528","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 119: Ausgabe 11-12, Seite 486-492 (2006)","doiTransmitted":false,"doiAuthor":"K. Hirsch, O. Simon, W. Vahjen","pdf":{"path":"http:\/\/data\/bmtw_2006_11_0486.pdf","title":"bmtw_2006_11_0486.pdf","description":"Einfluss eines Xylanase-Futterzusatzes auf Lactobacillus Spezies im Jejunum von Ferkeln

"},"authors":[{"firstName":"K","middleName":"","lastName":"Hirsch"},{"firstName":"O","middleName":"","lastName":"Simon"},{"firstName":"W","middleName":"","lastName":"Vahjen"}],"contentOptimised":"

Zusammenfassung<\/strong>
Acht Lactobacillus spp.-spezifische Oligonukleotidsonden wurden benutzt, um RNA-Extrakte aus dem Jejunum von Aufzuchtferkeln zu untersuchen, die mit einer Weizen\/Roggen basierten Di\u00e4t mit oder ohne Zusatz einer Xylanase gef\u00fcttert wurden. RNA Verd\u00fcnnungen aus Probengemischen oder Lactobacillus spp. Kulturen wurden verwendet, um Hybridisierungen mit Probenextrakten zu quantifizieren. Die Xylanase Supplementierung der Di\u00e4t f\u00fchrte zu einer signifikanten (p

Summary<\/strong>
rRNA extracts from the jejunum of rearing piglets fed a wheat\/ rye based diet with or without a xylanase feed additive were hybridized against eight Lactobacillus spp. specific digoxigenin labelled oligonucleotide probes. Known concentrati-ons of sample- or culture RNA extracts were used to calibrate and quantify hybri-dization signals from different sample extracts and hybridizations. The xylanase feed additive tended to increase total eubacterial rRNA in the termi-nal jejunum, probably a result of increased Lactobacillus spp. rRNA in the centra and terminal jejunum. However, individual animal variations were high. L reuten was the major Lactobacillus species followed by L acidophilus and L mucosae. Lamylophilus and L.crispatus were not detected in all RNA extracts.The results of this study show that the xylanase feed additive modifies Lactobacillus spp. popu-lations on the species level in piglets in addition to their well known effects on digesta viscosity and nutrient digestibility.The implications of these effects for the intestinal microbiota require further analysis.<\/p>","primaryLanguage":"deutsch","zusammenfassung":"Acht Lactobacillus spp.-spezifische Oligonukleotidsonden wurden benutzt, um RNA-Extrakte aus dem Jejunum von Aufzuchtferkeln zu untersuchen, die mit einer Weizen\/Roggen basierten Di\u00e4t mit oder ohne Zusatz einer Xylanase gef\u00fcttert wurden. RNA Verd\u00fcnnungen aus Probengemischen oder Lactobacillus spp. Kulturen wurden verwendet, um Hybridisierungen mit Probenextrakten zu quantifizieren. Die Xylanase Supplementierung der Di\u00e4t f\u00fchrte zu einer signifikanten (p","summary":"rRNA extracts from the jejunum of rearing piglets fed a wheat\/ rye based diet with or without a xylanase feed additive were hybridized against eight Lactobacillus spp. specific digoxigenin labelled oligonucleotide probes. Known concentrati-ons of sample- or culture RNA extracts were used to calibrate and quantify hybri-dization signals from different sample extracts and hybridizations. The xylanase feed additive tended to increase total eubacterial rRNA in the termi-nal jejunum, probably a result of increased Lactobacillus spp. rRNA in the centra and terminal jejunum. However, individual animal variations were high. L reuten was the major Lactobacillus species followed by L acidophilus and L mucosae. Lamylophilus and L.crispatus were not detected in all RNA extracts.The results of this study show that the xylanase feed additive modifies Lactobacillus spp. popu-lations on the species level in piglets in addition to their well known effects on digesta viscosity and nutrient digestibility.The implications of these effects for the intestinal microbiota require further analysis.<\/p>","doiLanguage":"deutsch","translatedTitle":"","abstractE":"Acht Lactobacillus spp.-spezifische Oligonukleotidsonden wurden benutzt, um RNA-Extrakte aus dem Jejunum von Aufzuchtferkeln zu untersuchen, die mit einer Weizen\/Roggen basierten Di\u00e4t mit oder ohne Zusatz einer Xylanase gef\u00fcttert wurden. RNA Verd\u00fcnnungen ...","date":{"year":2006,"date":"11\/2006","accepted":"2006-11-01"},"volume":"119","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["11761744\/150\/3130\/69528"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %8 11/2006