%0 Journal Article %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2009 %G English %I M. & H. Schaper GmbH %P 44-52 %R 10.2376/0341-6593-116-44 %T Determination of IgG and IgM levels in sera of newborn calves until the 10th day of life by ELISA and description of their correlation to total plasma protein concentration and GGT activity %V 116 %1 {"oldId":69353,"title":"Determination of IgG and IgM levels in sera of newborn calves until the 10th day of life by ELISA and description of their correlation to total plasma protein concentration and GGT activity","teaserText":"calf, colostrum, placental barrier, immunoglobulin, total plasmaprotein, GGTKalb, Kolostrum, Plazentarschranke, Immunglobulin,Gesamtprotein, GGT","content":"

Summary<\/span>
The aim of this study was the determination of IgG and IgM concentrations insera of 15 vital and healthy calves from the day of birth to the 10th day of lifeusing two ELISAs exclusively developed for this purpose. We investigated if andto which extent the sera profiles were correlated with antibody levels in thecolostral milk administered, with GGT activity and with total plasma proteincontent. Due to the assays\u2019 high sensitivity, traces of IgG and IgM in calf seracould be determined prior to the first uptake of the foremilk. Throughout thecolostrum administration period until the 12th living hour, IgG and IgM levelsremarkably increased (P

Zusammenfassung<\/span>
Ziel dieser Untersuchung war die Bestimmung von IgG- und IgM- Serumkonzentrationenim Serum von 15 vitalen und gesunden K\u00e4lbern vom Zeitpunkt derGeburt bis zum 10. Lebenstag. Dabei kamen zwei eigens daf\u00fcr entwickelte ELISAszur Anwendung. Die Beziehung der Immunglobulin\u2013Serumkonzentrationender Probanden 24 Stunden post natum zum Immunglobulingehalt der verabreichtenKolostralmilch, zur GGT\u2013Aktivit\u00e4t und zum Gesamtproteingehalt wurdebestimmt. Aufgrund der gro\u00dfen Sensitivit\u00e4t der Messmethoden konnten bereitsvor der ersten Kolostrumaufnahme geringe Mengen von IgG und IgM im Serumgefunden werden. Unter der Verabreichung der Kolostralmilch bis zur 12. Lebensstundestiegen die IgG\u2013 und IgM-Serumkonzentrationen deutlich an (P <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Feb 3, 2009 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/kalb-kolostrum-plazentarschranke-immunglobulin\/150\/3130\/69353"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"M. & H. Schaper GmbH","doiSerialWorkTitle":"Dtsch.tier\u00e4rztl.Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/kalb-kolostrum-plazentarschranke-immunglobulin\/150\/3130\/69353","doiSource":"Dtsch.tier\u00e4rztl.Wschr. 116: 2, 44-52 (2009)","doiissn":"0341-6593","doiNr":"10.2376\/0341-6593-116-44","doiFirstPage":"44","doiLastPage":"52","doiTransmitted":true,"doiAuthor":"Bender P, Bostedt H","pdf":{"path":"http:\/\/data\/dtw_2009_02_0044.pdf","title":"dtw_2009_02_0044.pdf","description":"Determination of IgG and IgM levels in sera of newborn calves until the 10th day of life by ELISA and description of their correlation to total plasma protein concentration and GGT activity

"},"authors":[{"firstName":"P","middleName":"","lastName":"Bender"},{"firstName":"H","middleName":"","lastName":"Bostedt"}],"contentOptimised":"

Summary<\/strong>
The aim of this study was the determination of IgG and IgM concentrations insera of 15 vital and healthy calves from the day of birth to the 10th day of lifeusing two ELISAs exclusively developed for this purpose. We investigated if andto which extent the sera profiles were correlated with antibody levels in thecolostral milk administered, with GGT activity and with total plasma proteincontent. Due to the assays\u2019 high sensitivity, traces of IgG and IgM in calf seracould be determined prior to the first uptake of the foremilk. Throughout thecolostrum administration period until the 12th living hour, IgG and IgM levelsremarkably increased (P

Zusammenfassung<\/strong>
Ziel dieser Untersuchung war die Bestimmung von IgG- und IgM- Serumkonzentrationenim Serum von 15 vitalen und gesunden K\u00e4lbern vom Zeitpunkt derGeburt bis zum 10. Lebenstag. Dabei kamen zwei eigens daf\u00fcr entwickelte ELISAszur Anwendung. Die Beziehung der Immunglobulin\u2013Serumkonzentrationender Probanden 24 Stunden post natum zum Immunglobulingehalt der verabreichtenKolostralmilch, zur GGT\u2013Aktivit\u00e4t und zum Gesamtproteingehalt wurdebestimmt. Aufgrund der gro\u00dfen Sensitivit\u00e4t der Messmethoden konnten bereitsvor der ersten Kolostrumaufnahme geringe Mengen von IgG und IgM im Serumgefunden werden. Unter der Verabreichung der Kolostralmilch bis zur 12. Lebensstundestiegen die IgG\u2013 und IgM-Serumkonzentrationen deutlich an (P <\/p>","primaryLanguage":"englisch","summary":"The aim of this study was the determination of IgG and IgM concentrations insera of 15 vital and healthy calves from the day of birth to the 10th day of lifeusing two ELISAs exclusively developed for this purpose. We investigated if andto which extent the sera profiles were correlated with antibody levels in thecolostral milk administered, with GGT activity and with total plasma proteincontent. Due to the assays\u2019 high sensitivity, traces of IgG and IgM in calf seracould be determined prior to the first uptake of the foremilk. Throughout thecolostrum administration period until the 12th living hour, IgG and IgM levelsremarkably increased (P","zusammenfassung":"Ziel dieser Untersuchung war die Bestimmung von IgG- und IgM- Serumkonzentrationenim Serum von 15 vitalen und gesunden K\u00e4lbern vom Zeitpunkt derGeburt bis zum 10. Lebenstag. Dabei kamen zwei eigens daf\u00fcr entwickelte ELISAszur Anwendung. Die Beziehung der Immunglobulin\u2013Serumkonzentrationender Probanden 24 Stunden post natum zum Immunglobulingehalt der verabreichtenKolostralmilch, zur GGT\u2013Aktivit\u00e4t und zum Gesamtproteingehalt wurdebestimmt. Aufgrund der gro\u00dfen Sensitivit\u00e4t der Messmethoden konnten bereitsvor der ersten Kolostrumaufnahme geringe Mengen von IgG und IgM im Serumgefunden werden. Unter der Verabreichung der Kolostralmilch bis zur 12. Lebensstundestiegen die IgG\u2013 und IgM-Serumkonzentrationen deutlich an (P <\/p>","translatedTitle":"calf, colostrum, placental barrier, immunoglobulin, total plasmaprotein, GGTKalb, Kolostrum, Plazentarschranke, Immunglobulin,Gesamtprotein, GGT","abstractE":"The aim of this study was the determination of IgG and IgM concentrations insera of 15 vital and healthy calves from the day of birth to the 10th day of lifeusing two ELISAs exclusively developed for this purpose. We investigated if andto which extent the sera profiles were correlated with antibody levels in thecolostral milk administered, with GGT activity and with total plasma proteincontent. Due to the assays\u2019 high sensitivity, traces of IgG and IgM in calf seracould be determined prior to the first uptake of the foremilk. Throughout thecolostrum administration period until the 12th living hour, IgG and IgM levelsremarkably increased (P","date":{"year":2009,"date":"02\/2009","accepted":"2009-02-03"},"volume":"116","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"44-52","redirects":["kalb-kolostrum-plazentarschranke-immunglobulin\/150\/3130\/69353"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %8 02/2009