%0 Journal Article %K Corynebacterium pseudotuberculosis %K recombinant phospholipase D %K whole cell antigens %K immunisation %K prevalence study %K caseous lymphadenitis %K ELISA %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2012 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 67-75 %R 10.2376/0005-9366-125-67 %T Serological studies on Corynebacterium pseudotuberculosis infections in goats in Baden-Wuerttemberg (Germany) and seroreactions on antigens used for newly developed Enzyme-Linked Immunosorbent Assays (ELISA) %V 125 %1 {"oldId":69062,"title":"Serological studies on Corynebacterium pseudotuberculosis infections in goats in Baden-Wuerttemberg (Germany) and seroreactions on antigens used for newly developed Enzyme-Linked Immunosorbent Assays (ELISA)","topline":"","teaserText":"In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on ...","content":"

Summary<\/span>
In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on a recombinant phospholipase D (rPLD) and whole cell antigens (WCA) were designed and validated yielding sensitivity values of 81% and 97% and specificity values of 98% and 99%, respectively.
Immunisation trials in goats demonstrated a significant production of antibodies to rPLD but an evidently lower antibody production to WCA as determined in the corresponding ELISA. Moreover, immunisation with rPLD resulted in the formation of antibodies, which were also detected in the WCA ELISA. In contrast, this phenomenon was not observed with the rPLD ELISA after immunisation with WCA.
Implementation of the rPLD and WCA ELISAs in a broad-based seroprevalence study in Baden-Wuerttemberg revealed positive reactions in both ELISAs in 13.2% of the 1771 goat sera tested. In 53.7% of 121 herds of which five or more animals were tested per herd there was at least one animal that showed a positive reaction in both tests.

Keywords:<\/span>
Corynebacterium pseudotuberculosis, recombinant phospholipase D, whole cell antigens, immunisation, prevalence study, caseous lymphadenitis, ELISA


Zusammenfassung<\/span>
In der vorliegenden Studie werden erstmals umfassende Daten zur Seropr\u00e4valenz von Corynebacterium (C.) pseudotuberculosis-Infektionen bei Ziegen in Baden- W\u00fcrttemberg (Deutschland) vorgestellt. Als Voraussetzung hierf\u00fcr wurden ELISASysteme auf der Basis einer rekombinanten Phospholipase D (rPLD) und von Ganzzellantigenen (WCA) entwickelt und validiert. Diese ELISAs erzielten jeweils Sensitivit\u00e4tswerte von 81 % und 97 % sowie Spezifit\u00e4tswerte von 98 % und 99 %. Immunisierungsversuche mit Ziegen zeigten im entsprechenden ELISA eine starke Bildung von Antik\u00f6rpern gegen rPLD, hingegen eine deutlich schw\u00e4chere Antik\u00f6rperbildung gegen WCA. Zudem hatten Impfungen mit rPLD die Bildung von Antik\u00f6rpern zur Folge, die auch im WCA-ELISA nachweisbar waren. Umgekehrt wurde dieses Ph\u00e4nomen im rPLD-ELISA nach Impfung mit WCA nicht beobachtet.
Die Anwendung des rPLD- und WCA-ELISA in einer breit angelegten Seropr\u00e4valenzstudie in Baden-W\u00fcrttemberg ergab positive Reaktionen in beiden ELISA Systemen bei 13,2% der insgesamt 1771 untersuchten Ziegenseren. In 53,7% der 121 Herden, aus denen f\u00fcnf oder mehr Tiere pro Herde untersucht wurden, zeigte mindestens ein Tier in beiden Tests eine positive Reaktion.

Schl\u00fcsselw\u00f6rter:<\/span>
Corynebacterium pseudotuberculosis, rekombinante Phospholipase D, Ganzzellantigene, Immunisierung, Pr\u00e4valenzstudie, Pseudotuberkulose, ELISA <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jan 10, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/corynebacterium-pseudotuberculosis-recombinant-phospholipase-d-whole-cell-antigens-immunisation-prevalence-study-caseous-lymphadenitis-elisa\/150\/3130\/69062"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/corynebacterium-pseudotuberculosis-recombinant-phospholipase-d-whole-cell-antigens-immunisation-prevalence-study-caseous-lymphadenitis-elisa\/150\/3130\/69062","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 1-2, 67-75 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-67","doiFirstPage":"67","doiLastPage":"75","doiTransmitted":true,"doiAuthor":"Sting R, Wagner B, Sari-Turan A, Stermann M, Reule M, Eichner M, Beyer W","pdf":{"path":"http:\/\/data\/bmtw_2012_01_0067.pdf","title":"bmtw_2012_01_0067.pdf","description":"Serological studies on Corynebacterium pseudotuberculosis infections in goats in Baden-Wuerttemberg (Germany) and seroreactions on antigens used for newly developed Enzyme-Linked Immunosorbent Assays (ELISA)

"},"authors":[{"firstName":"R","middleName":"","lastName":"Sting"},{"firstName":"B","middleName":"","lastName":"Wagner"},{"firstName":"A","middleName":"","lastName":"Sari-Turan"},{"firstName":"M","middleName":"","lastName":"Stermann"},{"firstName":"M","middleName":"","lastName":"Reule"},{"firstName":"M","middleName":"","lastName":"Eichner"},{"firstName":"W","middleName":"","lastName":"Beyer"}],"contentOptimised":"

Summary<\/strong>
In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on a recombinant phospholipase D (rPLD) and whole cell antigens (WCA) were designed and validated yielding sensitivity values of 81% and 97% and specificity values of 98% and 99%, respectively.
Immunisation trials in goats demonstrated a significant production of antibodies to rPLD but an evidently lower antibody production to WCA as determined in the corresponding ELISA. Moreover, immunisation with rPLD resulted in the formation of antibodies, which were also detected in the WCA ELISA. In contrast, this phenomenon was not observed with the rPLD ELISA after immunisation with WCA.
Implementation of the rPLD and WCA ELISAs in a broad-based seroprevalence study in Baden-Wuerttemberg revealed positive reactions in both ELISAs in 13.2% of the 1771 goat sera tested. In 53.7% of 121 herds of which five or more animals were tested per herd there was at least one animal that showed a positive reaction in both tests.

Keywords:<\/strong>
Corynebacterium pseudotuberculosis, recombinant phospholipase D, whole cell antigens, immunisation, prevalence study, caseous lymphadenitis, ELISA


Zusammenfassung<\/strong>
In der vorliegenden Studie werden erstmals umfassende Daten zur Seropr\u00e4valenz von Corynebacterium (C.) pseudotuberculosis-Infektionen bei Ziegen in Baden- W\u00fcrttemberg (Deutschland) vorgestellt. Als Voraussetzung hierf\u00fcr wurden ELISASysteme auf der Basis einer rekombinanten Phospholipase D (rPLD) und von Ganzzellantigenen (WCA) entwickelt und validiert. Diese ELISAs erzielten jeweils Sensitivit\u00e4tswerte von 81 % und 97 % sowie Spezifit\u00e4tswerte von 98 % und 99 %. Immunisierungsversuche mit Ziegen zeigten im entsprechenden ELISA eine starke Bildung von Antik\u00f6rpern gegen rPLD, hingegen eine deutlich schw\u00e4chere Antik\u00f6rperbildung gegen WCA. Zudem hatten Impfungen mit rPLD die Bildung von Antik\u00f6rpern zur Folge, die auch im WCA-ELISA nachweisbar waren. Umgekehrt wurde dieses Ph\u00e4nomen im rPLD-ELISA nach Impfung mit WCA nicht beobachtet.
Die Anwendung des rPLD- und WCA-ELISA in einer breit angelegten Seropr\u00e4valenzstudie in Baden-W\u00fcrttemberg ergab positive Reaktionen in beiden ELISA Systemen bei 13,2% der insgesamt 1771 untersuchten Ziegenseren. In 53,7% der 121 Herden, aus denen f\u00fcnf oder mehr Tiere pro Herde untersucht wurden, zeigte mindestens ein Tier in beiden Tests eine positive Reaktion.

Schl\u00fcsselw\u00f6rter:<\/strong>
Corynebacterium pseudotuberculosis, rekombinante Phospholipase D, Ganzzellantigene, Immunisierung, Pr\u00e4valenzstudie, Pseudotuberkulose, ELISA <\/p>","primaryLanguage":"englisch","summary":"In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on a recombinant phospholipase D (rPLD) and whole cell antigens (WCA) were designed and validated yielding sensitivity values of 81% and 97% and specificity values of 98% and 99%, respectively.
Immunisation trials in goats demonstrated a significant production of antibodies to rPLD but an evidently lower antibody production to WCA as determined in the corresponding ELISA. Moreover, immunisation with rPLD resulted in the formation of antibodies, which were also detected in the WCA ELISA. In contrast, this phenomenon was not observed with the rPLD ELISA after immunisation with WCA.
Implementation of the rPLD and WCA ELISAs in a broad-based seroprevalence study in Baden-Wuerttemberg revealed positive reactions in both ELISAs in 13.2% of the 1771 goat sera tested. In 53.7% of 121 herds of which five or more animals were tested per herd there was at least one animal that showed a positive reaction in both tests.","keywords":["Corynebacterium pseudotuberculosis","recombinant phospholipase D","whole cell antigens","immunisation","prevalence study","caseous lymphadenitis","ELISA"],"zusammenfassung":"In der vorliegenden Studie werden erstmals umfassende Daten zur Seropr\u00e4valenz von Corynebacterium (C.) pseudotuberculosis-Infektionen bei Ziegen in Baden- W\u00fcrttemberg (Deutschland) vorgestellt. Als Voraussetzung hierf\u00fcr wurden ELISASysteme auf der Basis einer rekombinanten Phospholipase D (rPLD) und von Ganzzellantigenen (WCA) entwickelt und validiert. Diese ELISAs erzielten jeweils Sensitivit\u00e4tswerte von 81 % und 97 % sowie Spezifit\u00e4tswerte von 98 % und 99 %. Immunisierungsversuche mit Ziegen zeigten im entsprechenden ELISA eine starke Bildung von Antik\u00f6rpern gegen rPLD, hingegen eine deutlich schw\u00e4chere Antik\u00f6rperbildung gegen WCA. Zudem hatten Impfungen mit rPLD die Bildung von Antik\u00f6rpern zur Folge, die auch im WCA-ELISA nachweisbar waren. Umgekehrt wurde dieses Ph\u00e4nomen im rPLD-ELISA nach Impfung mit WCA nicht beobachtet.
Die Anwendung des rPLD- und WCA-ELISA in einer breit angelegten Seropr\u00e4valenzstudie in Baden-W\u00fcrttemberg ergab positive Reaktionen in beiden ELISA Systemen bei 13,2% der insgesamt 1771 untersuchten Ziegenseren. In 53,7% der 121 Herden, aus denen f\u00fcnf oder mehr Tiere pro Herde untersucht wurden, zeigte mindestens ein Tier in beiden Tests eine positive Reaktion.","schluesselwoerter":["Corynebacterium pseudotuberculosis","rekombinante Phospholipase D","Ganzzellantigene","Immunisierung","Pr\u00e4valenzstudie","Pseudotuberkulose","ELISA"],"translatedTitle":"In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on ...","abstractE":"In the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on a recombinant phospholipase D (rPLD) and whole cell antigens (WCA) were designed and validated yielding sensitivity values of 81% and 97% and specificity values of 98% and 99%, respectively. Immunisation trials in goats demonstrated a significant production of antibodies to rPLD but an evidently lower antibody production to WCA as determined in the corresponding ELISA. Moreover, immunisation with rPLD resulted in the formation of antibodies, which were also detected in the WCA ELISA. In contrast, this phenomenon was not observed with the rPLD ELISA after immunisation with WCA. Implementation of the rPLD and WCA ELISAs in a broad-based seroprevalence study in Baden-Wuerttemberg revealed positive reactions in both ELISAs in 13.2% of the 1771 goat sera tested. In 53.7% of 121 herds of which five or more animals were tested per herd there was at least one animal that showed a positive reaction in both tests.","date":{"year":2012,"date":"01\/2012","accepted":"2012-01-10"},"volume":"125","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"67-75","redirects":["corynebacterium-pseudotuberculosis-recombinant-phospholipase-d-whole-cell-antigens-immunisation-prevalence-study-caseous-lymphadenitis-elisa\/150\/3130\/69062"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %8 01/2012