%0 Journal Article %K Vibrio spp. %K prevalence %K virulence %K antimicrobial resistance %K shrimps %B Berliner und Münchener Tierärztliche Wochenschrift %C Hannover %D 2016 %G English %I Schlütersche Verlagsgesellschaft mbH & Co. KG %P 48-51 %R 10.2376/0005-9366-129-48 %T Prevalence and antimicrobial resistance of Vibrio spp. in retail shrimps in Vietnam %V 129 %1 {"oldId":92780,"title":"Prevalence and antimicrobial resistance of Vibrio spp. in retail shrimps in Vietnam","topline":"","teaserText":"Pr\u00e4valenz und antimikrobielle Resistenz von Vibrio spp. in Shrimps von M\u00e4rkten in Vietnam","content":"

Summary<\/span>
The aim of the study was to investigate the prevalence and the antimicrobial resistance patterns of Vibrio (V.) spp. isolated from retail shrimp in Hanoi, Vietnam. A total of 202 shrimp samples were collected from retail markets located in ten urban districts of Hanoi. Among those, 201 (99.5%) samples were positive for Vibrio spp. The most common species detected was V. parahaemolyticus (96.5%), followed by V. alginolyticus (56.4%), V. cholerae (2%) and V. vulnificus (1.5%). Multiple Vibrio spp. were found in 114 (56.4%) samples. None of the V. parahaemolyticus isolates carried the virulence-associated tdh (thermostable direct haemolysin) and trh (tdh-related haemolysin) genes. In total, 195 V. parahaemolyticus isolates, four V. cholerae isolates and three V. vulnificus isolates were tested for resistance to eight antimicrobial agents. V. parahaemolyticus isolates showed high rates of resistance against ampicillin (87.2%), while a moderate rate was observed for sulfamethoxazole\/trimethoprim (18.5%) and intermediate resistance towards tetracycline (24.6%). Low resistance rates (0.5%) were recorded against both ciprofloxacin and cefalothin. Only one V. cholerae isolate with resistance to ampicillin and two V. cholerae isolates with resistance to sulfamethoxazole\/trimethoprim were found. All V. vulnificus isolates were susceptible to the eight antimicrobial agents tested. However, the number of V. vulnificus and V. cholerae was small. Multi-resistant isolates were found in V. parahaemolyticus with a low frequency (16.9%). The results of this study revealed the ubiquitous nature of Vibrio spp. in shrimp at retail. To reduce the potential risk of Vibrio infections due to handling or consumption of undercooked seafood, good manufacturing practice as well as safe handling and processing should be encouraged.<\/p>

Keywords<\/span>
Vibrio spp., prevalence, virulence, antimicrobial resistance, shrimps<\/p>

Zusammenfassung<\/span>
Ziel der Studie war die Untersuchung von Shrimps aus dem Einzelhandel in Hanoi, Vietnam hinsichtlich der Vibrio (V.) spp. Pr\u00e4valenz und deren antimikrobiellen Resistenzen. Insgesamt wurden 202 Einzelproben von M\u00e4rkten aus zehn Bezirken Hanois, Vietnam, analysiert. Dabei wurden 201 (99,5 %) der Proben positiv auf Vibrio spp. getestet. Am h\u00e4ufigsten wurde die Spezies V. parahaemolyticus (96,5 %) detektiert, gefolgt von V. alginolyticus (56,4 %), V. cholerae (2 %) und V. vulnificus (1,5 %). Mehrere Spezies wurden in 144 (56,4 %) der Proben gefunden. Keines der V. parahaemolyticus-Isolate codierte die Virulenz-assoziierten Gene tdh (thermostabiles direktes H\u00e4molysin) bzw. trh (tdh-verwandtes H\u00e4molysin). Insgesamt wurden 195 V. parahaemolyticus-Isolate, vier V. cholerae-Isolate und drei V. vulnificus-Isolate auf antimikrobielle Resistenzen gegen\u00fcber acht Antibiotika getestet. V. parahaemolyticus-Isolate wiesen eine hohe Resistenzrate gegen Ampicillin (87,2 %) auf. Gegen\u00fcber Tetracyclin wiesen 24,6 % der Isolate Resistenzen auf, wohingegen nur 36 Isolate (18,5 %) Sulfamethoxazol\/Trimethoprim-Resistenz zeigten. Niedrige Resistenzraten (0,5 %) wurden f\u00fcr Ciprofloxacin und Cefalothin detektiert. Lediglich ein V. cholerae-Isolat wies Resistenzen gegen\u00fcber Ampicillin auf. Zwei weitere V. cholerae-Isolate zeigten Resistenzen gegen\u00fcber Sulfamethoxazol\/Trimethoprim. Alle untersuchten V. vulnificus-Isolate waren f\u00fcr alle acht Antibiotika sensibel. Ein geringer Anteil der V. parahaemolyticus-Isolate (16,9 %) wies Multiresistenzen auf. Diese Studie weist auf die ubiquit\u00e4re Verbreitung von Vibrio spp. in Shrimps aus dem Handel in Vietnam hin. Um das Risiko von Vibrio-Infektionen durch Konsum von nicht ausreichend erhitzten Meeresfr\u00fcchten zu reduzieren, sollte auf eine gute Herstellungspraxis sowie einen sicheren Umgang und entsprechende Verarbeitungsschritte geachtet werden.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Vibrio spp., Pr\u00e4valenz, Virulenz, antimikrobielle Resistenz, Shrimps<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jan 18, 2016 7:08:28 PM","oldUrls":["http:\/\/vetline.de\/prevalence-and-antimicrobial-resistance-of-vibrio-spp-in-retail-shrimps-in-vietnam\/150\/3130\/92780"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/prevalence-and-antimicrobial-resistance-of-vibrio-spp-in-retail-shrimps-in-vietnam\/150\/3130\/92780\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 129, 48\u201351 (2016) ","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-129-48","doiFirstPage":"48","doiLastPage":"51","doiTransmitted":true,"doiAuthor":"Thi Thu Tra V, Meng L, Pichpol D, Hong Pham N, Baumann M, Alter T, Huehn S","pdf":{"path":"http:\/\/data\/BMW_2016_01_02_0048.pdf","title":"BMW_2016_01_02_0048.pdf","description":"Prevalence and antimicrobial resistance of Vibrio spp. in retail shrimps in Vietnam"},"authors":[{"firstName":"V","middleName":"","lastName":"Thi Thu Tra"},{"firstName":"L","middleName":"","lastName":"Meng"},{"firstName":"D","middleName":"","lastName":"Pichpol"},{"firstName":"N","middleName":"","lastName":"Hong Pham"},{"firstName":"M","middleName":"","lastName":"Baumann"},{"firstName":"T","middleName":"","lastName":"Alter"},{"firstName":"S","middleName":"","lastName":"Huehn"}],"contentOptimised":"

Summary<\/strong>
The aim of the study was to investigate the prevalence and the antimicrobial resistance patterns of Vibrio (V.) spp. isolated from retail shrimp in Hanoi, Vietnam. A total of 202 shrimp samples were collected from retail markets located in ten urban districts of Hanoi. Among those, 201 (99.5%) samples were positive for Vibrio spp. The most common species detected was V. parahaemolyticus (96.5%), followed by V. alginolyticus (56.4%), V. cholerae (2%) and V. vulnificus (1.5%). Multiple Vibrio spp. were found in 114 (56.4%) samples. None of the V. parahaemolyticus isolates carried the virulence-associated tdh (thermostable direct haemolysin) and trh (tdh-related haemolysin) genes. In total, 195 V. parahaemolyticus isolates, four V. cholerae isolates and three V. vulnificus isolates were tested for resistance to eight antimicrobial agents. V. parahaemolyticus isolates showed high rates of resistance against ampicillin (87.2%), while a moderate rate was observed for sulfamethoxazole\/trimethoprim (18.5%) and intermediate resistance towards tetracycline (24.6%). Low resistance rates (0.5%) were recorded against both ciprofloxacin and cefalothin. Only one V. cholerae isolate with resistance to ampicillin and two V. cholerae isolates with resistance to sulfamethoxazole\/trimethoprim were found. All V. vulnificus isolates were susceptible to the eight antimicrobial agents tested. However, the number of V. vulnificus and V. cholerae was small. Multi-resistant isolates were found in V. parahaemolyticus with a low frequency (16.9%). The results of this study revealed the ubiquitous nature of Vibrio spp. in shrimp at retail. To reduce the potential risk of Vibrio infections due to handling or consumption of undercooked seafood, good manufacturing practice as well as safe handling and processing should be encouraged.<\/p>

Keywords:<\/strong>
Vibrio spp., prevalence, virulence, antimicrobial resistance, shrimps<\/p>

Zusammenfassung<\/strong>
Ziel der Studie war die Untersuchung von Shrimps aus dem Einzelhandel in Hanoi, Vietnam hinsichtlich der Vibrio (V.) spp. Pr\u00e4valenz und deren antimikrobiellen Resistenzen. Insgesamt wurden 202 Einzelproben von M\u00e4rkten aus zehn Bezirken Hanois, Vietnam, analysiert. Dabei wurden 201 (99,5 %) der Proben positiv auf Vibrio spp. getestet. Am h\u00e4ufigsten wurde die Spezies V. parahaemolyticus (96,5 %) detektiert, gefolgt von V. alginolyticus (56,4 %), V. cholerae (2 %) und V. vulnificus (1,5 %). Mehrere Spezies wurden in 144 (56,4 %) der Proben gefunden. Keines der V. parahaemolyticus-Isolate codierte die Virulenz-assoziierten Gene tdh (thermostabiles direktes H\u00e4molysin) bzw. trh (tdh-verwandtes H\u00e4molysin). Insgesamt wurden 195 V. parahaemolyticus-Isolate, vier V. cholerae-Isolate und drei V. vulnificus-Isolate auf antimikrobielle Resistenzen gegen\u00fcber acht Antibiotika getestet. V. parahaemolyticus-Isolate wiesen eine hohe Resistenzrate gegen Ampicillin (87,2 %) auf. Gegen\u00fcber Tetracyclin wiesen 24,6 % der Isolate Resistenzen auf, wohingegen nur 36 Isolate (18,5 %) Sulfamethoxazol\/Trimethoprim-Resistenz zeigten. Niedrige Resistenzraten (0,5 %) wurden f\u00fcr Ciprofloxacin und Cefalothin detektiert. Lediglich ein V. cholerae-Isolat wies Resistenzen gegen\u00fcber Ampicillin auf. Zwei weitere V. cholerae-Isolate zeigten Resistenzen gegen\u00fcber Sulfamethoxazol\/Trimethoprim. Alle untersuchten V. vulnificus-Isolate waren f\u00fcr alle acht Antibiotika sensibel. Ein geringer Anteil der V. parahaemolyticus-Isolate (16,9 %) wies Multiresistenzen auf. Diese Studie weist auf die ubiquit\u00e4re Verbreitung von Vibrio spp. in Shrimps aus dem Handel in Vietnam hin. Um das Risiko von Vibrio-Infektionen durch Konsum von nicht ausreichend erhitzten Meeresfr\u00fcchten zu reduzieren, sollte auf eine gute Herstellungspraxis sowie einen sicheren Umgang und entsprechende Verarbeitungsschritte geachtet werden.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Vibrio spp., Pr\u00e4valenz, Virulenz, antimikrobielle Resistenz, Shrimps<\/p>","primaryLanguage":"englisch","summary":"The aim of the study was to investigate the prevalence and the antimicrobial resistance patterns of Vibrio (V.) spp. isolated from retail shrimp in Hanoi, Vietnam. A total of 202 shrimp samples were collected from retail markets located in ten urban districts of Hanoi. Among those, 201 (99.5%) samples were positive for Vibrio spp. The most common species detected was V. parahaemolyticus (96.5%), followed by V. alginolyticus (56.4%), V. cholerae (2%) and V. vulnificus (1.5%). Multiple Vibrio spp. were found in 114 (56.4%) samples. None of the V. parahaemolyticus isolates carried the virulence-associated tdh (thermostable direct haemolysin) and trh (tdh-related haemolysin) genes. In total, 195 V. parahaemolyticus isolates, four V. cholerae isolates and three V. vulnificus isolates were tested for resistance to eight antimicrobial agents. V. parahaemolyticus isolates showed high rates of resistance against ampicillin (87.2%), while a moderate rate was observed for sulfamethoxazole\/trimethoprim (18.5%) and intermediate resistance towards tetracycline (24.6%). Low resistance rates (0.5%) were recorded against both ciprofloxacin and cefalothin. Only one V. cholerae isolate with resistance to ampicillin and two V. cholerae isolates with resistance to sulfamethoxazole\/trimethoprim were found. All V. vulnificus isolates were susceptible to the eight antimicrobial agents tested. However, the number of V. vulnificus and V. cholerae was small. Multi-resistant isolates were found in V. parahaemolyticus with a low frequency (16.9%). The results of this study revealed the ubiquitous nature of Vibrio spp. in shrimp at retail. To reduce the potential risk of Vibrio infections due to handling or consumption of undercooked seafood, good manufacturing practice as well as safe handling and processing should be encouraged.<\/p>

","keywords":["Vibrio spp.","prevalence","virulence","antimicrobial resistance","shrimps"],"zusammenfassung":"Ziel der Studie war die Untersuchung von Shrimps aus dem Einzelhandel in Hanoi, Vietnam hinsichtlich der Vibrio (V.) spp. Pr\u00e4valenz und deren antimikrobiellen Resistenzen. Insgesamt wurden 202 Einzelproben von M\u00e4rkten aus zehn Bezirken Hanois, Vietnam, analysiert. Dabei wurden 201 (99,5 %) der Proben positiv auf Vibrio spp. getestet. Am h\u00e4ufigsten wurde die Spezies V. parahaemolyticus (96,5 %) detektiert, gefolgt von V. alginolyticus (56,4 %), V. cholerae (2 %) und V. vulnificus (1,5 %). Mehrere Spezies wurden in 144 (56,4 %) der Proben gefunden. Keines der V. parahaemolyticus-Isolate codierte die Virulenz-assoziierten Gene tdh (thermostabiles direktes H\u00e4molysin) bzw. trh (tdh-verwandtes H\u00e4molysin). Insgesamt wurden 195 V. parahaemolyticus-Isolate, vier V. cholerae-Isolate und drei V. vulnificus-Isolate auf antimikrobielle Resistenzen gegen\u00fcber acht Antibiotika getestet. V. parahaemolyticus-Isolate wiesen eine hohe Resistenzrate gegen Ampicillin (87,2 %) auf. Gegen\u00fcber Tetracyclin wiesen 24,6 % der Isolate Resistenzen auf, wohingegen nur 36 Isolate (18,5 %) Sulfamethoxazol\/Trimethoprim-Resistenz zeigten. Niedrige Resistenzraten (0,5 %) wurden f\u00fcr Ciprofloxacin und Cefalothin detektiert. Lediglich ein V. cholerae-Isolat wies Resistenzen gegen\u00fcber Ampicillin auf. Zwei weitere V. cholerae-Isolate zeigten Resistenzen gegen\u00fcber Sulfamethoxazol\/Trimethoprim. Alle untersuchten V. vulnificus-Isolate waren f\u00fcr alle acht Antibiotika sensibel. Ein geringer Anteil der V. parahaemolyticus-Isolate (16,9 %) wies Multiresistenzen auf. Diese Studie weist auf die ubiquit\u00e4re Verbreitung von Vibrio spp. in Shrimps aus dem Handel in Vietnam hin. Um das Risiko von Vibrio-Infektionen durch Konsum von nicht ausreichend erhitzten Meeresfr\u00fcchten zu reduzieren, sollte auf eine gute Herstellungspraxis sowie einen sicheren Umgang und entsprechende Verarbeitungsschritte geachtet werden.<\/p>

","schluesselwoerter":["Vibrio spp.","Pr\u00e4valenz","Virulenz","antimikrobielle Resistenz","Shrimps"],"translatedTitle":"Pr\u00e4valenz und antimikrobielle Resistenz von Vibrio spp. in Shrimps von M\u00e4rkten in Vietnam","abstractE":"The aim of the study was to investigate the prevalence and the antimicrobial resistance patterns of Vibrio (V.) spp. isolated from retail shrimp in Hanoi, Vietnam. A total of 202 shrimp samples were collected from retail markets located in ten urban districts of Hanoi. Among those, 201 (99.5%) samples were positive for Vibrio spp. The most common species detected was V. parahaemolyticus (96.5%), followed by V. alginolyticus (56.4%), V. cholerae (2%) and V. vulnificus (1.5%). Multiple Vibrio spp. were found in 114 (56.4%) samples. None of the V. parahaemolyticus isolates carried the virulence-associated tdh (thermostable direct haemolysin) and trh (tdh-related haemolysin) genes. In total, 195 V. parahaemolyticus isolates, four V. cholerae isolates and three V. vulnificus isolates were tested for resistance to eight antimicrobial agents. V. parahaemolyticus isolates showed high rates of resistance against ampicillin (87.2%), while a moderate rate was observed for sulfamethoxazole\/trimethoprim (18.5%) and intermediate resistance towards tetracycline (24.6%). Low resistance rates (0.5%) were recorded against both ciprofloxacin and cefalothin. Only one V. cholerae isolate with resistance to ampicillin and two V. cholerae isolates with resistance to sulfamethoxazole\/trimethoprim were found. All V. vulnificus isolates were susceptible to the eight antimicrobial agents tested. However, the number of V. vulnificus and V. cholerae was small. Multi-resistant isolates were found in V. parahaemolyticus with a low frequency (16.9%). The results of this study revealed the ubiquitous nature of Vibrio spp. in shrimp at retail. To reduce the potential risk of Vibrio infections due to handling or consumption of undercooked seafood, good manufacturing practice as well as safe handling and processing should be encouraged.","date":{"year":2016,"date":"01\/2016","accepted":"2016-01-18"},"volume":"129","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"48-51","redirects":["prevalence-and-antimicrobial-resistance-of-vibrio-spp-in-retail-shrimps-in-vietnam\/150\/3130\/92780"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} %7 1/2 %8 01/2016