%0 Journal Article
%K Arcobacter
%K food
%K prevalence
%K genotype
%K virulence genes
%B Berliner und Münchener Tierärztliche Wochenschrift
%C Hannover
%D 2014
%G English
%I Schlütersche Verlagsgesellschaft mbH & Co. KG
%P 163-168
%R 10.2376/0005-9366-128-163
%T Prevalence, virulence gene distribution and genetic diversity of Arcobacter in food samples in Germany
%V 128
%1 {"oldId":84358,"title":"Prevalence, virulence gene distribution and genetic diversity of Arcobacter in food samples in Germany","topline":"Open Access | CARO-Themenheft","teaserText":"Zur Pr\u00e4valenz, der Verteilung von Virulenzgenen und der genetischen Diversit\u00e4t von Arcobacter aus Lebensmitteln in Deutschland","content":"<p><span class=\"bold\">Summary<\/span><\/p><p> This study was carried out to determine the prevalence of<span class=\"bold\"> <\/span><span class=\"italic\">Arcobacter<span class=\"bold\"> <\/span><\/span>spp. in food  samples in Germany. In addition, the presence of putative virulence genes and  the genetic diversity was tested for <span class=\"italic\">Arcobacter (A.) butzleri <\/span>strains isolated during  this study. The prevalence of <span class=\"italic\">Arcobacter <\/span>spp. was 34% in fish meat, 26.8% in  poultry meat and 2% in minced meat (beef and pork). All investigated <span class=\"italic\">A. butzleri<\/span>  isolates carried the genes <span class=\"italic\">ca<\/span><span class=\"italic\">dF, ciaB, cj1349, mviN <\/span>and <span class=\"italic\">pldA<\/span>. The gene <span class=\"italic\">tlyA<\/span> was  detectable in 97.5% of the strains. Lower detection rates were observed for <span class=\"italic\">hecA<\/span>  (47.5%), <span class=\"italic\">hecB<\/span> (45%), <span class=\"italic\">iroE<\/span> (40%) and <span class=\"italic\">irgA<\/span> (35%). Genotyping by ERIC-PCR demonstrated  a high genetic diversity of <span class=\"italic\">A. butzleri<\/span> strains from different foods. In  conclusion, this study shows that about one third of fish meat and poultry meat  samples contained <span class=\"italic\">Arcobacter<\/span> spp. These data highlight the need to strengthen  our effort to elucidate the importance of Arcobacter on veterinary public health.<\/p><p> <span class=\"bold\">Keywords<\/span><\/p><p> <span class=\"italic\">Arcobacter,<\/span> food, prevalence, genotype, virulence genes<\/p><p><span class=\"bold\">Zusammenfassung<\/span><\/p><p> Ziel der Studie war, die Pr\u00e4valenz von <span class=\"italic\">Arcobacter<\/span> spp. in Lebensmittelproben  in Deutschland zu bestimmen. Zus\u00e4tzlich wurde die Anwesenheit putativer  Virulenzgene und die genetische Diversit\u00e4t der <span class=\"italic\">Arcobacter (A.) butzleri<\/span>-St\u00e4mme  untersucht. Die Pr\u00e4valenz von <span class=\"italic\">Arcobacter<\/span> spp. betrug 34 % in Fisch, 26,8 % in  Gefl\u00fcgelfleisch und 2 % in Hackfleisch (Rindfleisch und Schweinefleisch). In allen  untersuchten <span class=\"italic\">A. butzleri-<\/span>Isolaten waren die Gene <span class=\"italic\">cadF, ciaB, cj1349, mviN <\/span>und <span class=\"italic\">pldA<\/span> nachweisbar. Das Gen <span class=\"italic\">tlyA <\/span>war in 97,5 % der St\u00e4mme zu finden. F\u00fcr <span class=\"italic\">hecA <\/span> (47.5 %), <span class=\"italic\">hecB<\/span> (45 %),<span class=\"italic\"> iroE<\/span> (40 %) und <span class=\"italic\">irgA (<\/span>35 %) wurden niedrigere Nachweisraten  beobachtet. Die Genotypisierung der <span class=\"italic\">A. butzleri-<\/span>St\u00e4mme aus verschiedenen  Lebensmitteln mittels ERIC-PCR zeigte eine hohe genetische Vielfalt dieser  St\u00e4mme. Zusammenfassend belegt diese Studie, dass etwa ein Drittel der Fisch- und  Gefl\u00fcgelfleischproben <span class=\"italic\">Arcobacter<\/span> spp. enthielten. Diese Daten unterstreichen  die Notwendigkeit, die Bedeutung von <span class=\"italic\">Arcobacter<\/span> f\u00fcr den gesundheitlichen  Verbraucherschutz weitergehend zu betrachten.<\/p><p> <span class=\"bold\">Schl\u00fcsselw\u00f6rter<\/span><\/p><p><span class=\"italic\">Arcobacter<\/span>, Lebensmittel, Pr\u00e4valenz, Genotyp, Virulenzgene<\/p>","categories":["Open Access","Abostufe frei"],"fromDate":"Dec 22, 2014 8:49:39 AM","oldUrls":["http:\/\/vetline.de\/prevalence-virulence-gene-distribution-and-genetic-diversity-of-arcobacter-in-food-samples-in-germany\/150\/3216\/84358"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr","doiDocumentUri":"http:\/\/vetline.de\/prevalence-virulence-gene-distribution-and-genetic-diversity-of-arcobacter-in-food-samples-in-germany\/150\/3216\/84358\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 163\u2013168","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-163","doiFirstPage":"163","doiLastPage":"168","doiTransmitted":true,"doiAuthor":"Lehmann D, Alter T, Lehmann L, Uherkova S, Seidler T, G\u00f6lz G","pdf":{"path":"http:\/\/data\/BMW_2015_0163.pdf","title":"BMW_2015_0163.pdf","description":"Prevalence, virulence gene distribution and genetic diversity of Arcobacter in food samples in Germany"},"authors":[{"firstName":"D","middleName":"","lastName":"Lehmann"},{"firstName":"T","middleName":"","lastName":"Alter"},{"firstName":"L","middleName":"","lastName":"Lehmann"},{"firstName":"S","middleName":"","lastName":"Uherkova"},{"firstName":"T","middleName":"","lastName":"Seidler"},{"firstName":"G","middleName":"","lastName":"G\u00f6lz"}],"contentOptimised":"<p><strong>Summary<\/strong><\/p><p> This study was carried out to determine the prevalence of<strong> <\/strong><em>Arcobacter<strong> <\/strong><\/em>spp. in food  samples in Germany. In addition, the presence of putative virulence genes and  the genetic diversity was tested for <em>Arcobacter (A.) butzleri <\/em>strains isolated during  this study. The prevalence of <em>Arcobacter <\/em>spp. was 34% in fish meat, 26.8% in  poultry meat and 2% in minced meat (beef and pork). All investigated <em>A. butzleri<\/em>  isolates carried the genes <em>ca<\/em><em>dF, ciaB, cj1349, mviN <\/em>and <em>pldA<\/em>. The gene <em>tlyA<\/em> was  detectable in 97.5% of the strains. Lower detection rates were observed for <em>hecA<\/em>  (47.5%), <em>hecB<\/em> (45%), <em>iroE<\/em> (40%) and <em>irgA<\/em> (35%). Genotyping by ERIC-PCR demonstrated  a high genetic diversity of <em>A. butzleri<\/em> strains from different foods. In  conclusion, this study shows that about one third of fish meat and poultry meat  samples contained <em>Arcobacter<\/em> spp. These data highlight the need to strengthen  our effort to elucidate the importance of Arcobacter on veterinary public health.<\/p><p> <strong>Keywords:<\/strong><\/p><p> <em>Arcobacter,<\/em> food, prevalence, genotype, virulence genes<\/p><p><strong>Zusammenfassung<\/strong><\/p><p> Ziel der Studie war, die Pr\u00e4valenz von <em>Arcobacter<\/em> spp. in Lebensmittelproben  in Deutschland zu bestimmen. Zus\u00e4tzlich wurde die Anwesenheit putativer  Virulenzgene und die genetische Diversit\u00e4t der <em>Arcobacter (A.) butzleri<\/em>-St\u00e4mme  untersucht. Die Pr\u00e4valenz von <em>Arcobacter<\/em> spp. betrug 34 % in Fisch, 26,8 % in  Gefl\u00fcgelfleisch und 2 % in Hackfleisch (Rindfleisch und Schweinefleisch). In allen  untersuchten <em>A. butzleri-<\/em>Isolaten waren die Gene <em>cadF, ciaB, cj1349, mviN <\/em>und <em>pldA<\/em> nachweisbar. Das Gen <em>tlyA <\/em>war in 97,5 % der St\u00e4mme zu finden. F\u00fcr <em>hecA <\/em> (47.5 %), <em>hecB<\/em> (45 %),<em> iroE<\/em> (40 %) und <em>irgA (<\/em>35 %) wurden niedrigere Nachweisraten  beobachtet. Die Genotypisierung der <em>A. butzleri-<\/em>St\u00e4mme aus verschiedenen  Lebensmitteln mittels ERIC-PCR zeigte eine hohe genetische Vielfalt dieser  St\u00e4mme. Zusammenfassend belegt diese Studie, dass etwa ein Drittel der Fisch- und  Gefl\u00fcgelfleischproben <em>Arcobacter<\/em> spp. enthielten. Diese Daten unterstreichen  die Notwendigkeit, die Bedeutung von <em>Arcobacter<\/em> f\u00fcr den gesundheitlichen  Verbraucherschutz weitergehend zu betrachten.<\/p><p> <strong>Schl\u00fcsselw\u00f6rter:<\/strong><\/p><p><em>Arcobacter<\/em>, Lebensmittel, Pr\u00e4valenz, Genotyp, Virulenzgene<\/p>","primaryLanguage":"englisch","summary":"<\/p><p> This study was carried out to determine the prevalence of<strong> <\/strong><em>Arcobacter<strong> <\/strong><\/em>spp. in food  samples in Germany. In addition, the presence of putative virulence genes and  the genetic diversity was tested for <em>Arcobacter (A.) butzleri <\/em>strains isolated during  this study. The prevalence of <em>Arcobacter <\/em>spp. was 34% in fish meat, 26.8% in  poultry meat and 2% in minced meat (beef and pork). All investigated <em>A. butzleri<\/em>  isolates carried the genes <em>ca<\/em><em>dF, ciaB, cj1349, mviN <\/em>and <em>pldA<\/em>. The gene <em>tlyA<\/em> was  detectable in 97.5% of the strains. Lower detection rates were observed for <em>hecA<\/em>  (47.5%), <em>hecB<\/em> (45%), <em>iroE<\/em> (40%) and <em>irgA<\/em> (35%). Genotyping by ERIC-PCR demonstrated  a high genetic diversity of <em>A. butzleri<\/em> strains from different foods. In  conclusion, this study shows that about one third of fish meat and poultry meat  samples contained <em>Arcobacter<\/em> spp. These data highlight the need to strengthen  our effort to elucidate the importance of Arcobacter on veterinary public health.<\/p><p>","keywords":["Arcobacter","food","prevalence","genotype","virulence genes"],"zusammenfassung":"<\/p><p> Ziel der Studie war, die Pr\u00e4valenz von <em>Arcobacter<\/em> spp. in Lebensmittelproben  in Deutschland zu bestimmen. Zus\u00e4tzlich wurde die Anwesenheit putativer  Virulenzgene und die genetische Diversit\u00e4t der <em>Arcobacter (A.) butzleri<\/em>-St\u00e4mme  untersucht. Die Pr\u00e4valenz von <em>Arcobacter<\/em> spp. betrug 34 % in Fisch, 26,8 % in  Gefl\u00fcgelfleisch und 2 % in Hackfleisch (Rindfleisch und Schweinefleisch). In allen  untersuchten <em>A. butzleri-<\/em>Isolaten waren die Gene <em>cadF, ciaB, cj1349, mviN <\/em>und <em>pldA<\/em> nachweisbar. Das Gen <em>tlyA <\/em>war in 97,5 % der St\u00e4mme zu finden. F\u00fcr <em>hecA <\/em> (47.5 %), <em>hecB<\/em> (45 %),<em> iroE<\/em> (40 %) und <em>irgA (<\/em>35 %) wurden niedrigere Nachweisraten  beobachtet. Die Genotypisierung der <em>A. butzleri-<\/em>St\u00e4mme aus verschiedenen  Lebensmitteln mittels ERIC-PCR zeigte eine hohe genetische Vielfalt dieser  St\u00e4mme. Zusammenfassend belegt diese Studie, dass etwa ein Drittel der Fisch- und  Gefl\u00fcgelfleischproben <em>Arcobacter<\/em> spp. enthielten. Diese Daten unterstreichen  die Notwendigkeit, die Bedeutung von <em>Arcobacter<\/em> f\u00fcr den gesundheitlichen  Verbraucherschutz weitergehend zu betrachten.<\/p><p>","schluesselwoerter":["Arcobacter","Lebensmittel","Pr\u00e4valenz","Genotyp","Virulenzgene"],"translatedTitle":"Zur Pr\u00e4valenz, der Verteilung von Virulenzgenen und der genetischen Diversit\u00e4t von Arcobacter aus Lebensmitteln in Deutschland","abstractE":" This study was carried out to determine the prevalence of Arcobacter spp. in food  samples in Germany. In addition, the presence of putative virulence genes and  the genetic diversity was tested for Arcobacter (A.) butzleri strains isolated during  this study. The prevalence of Arcobacter spp. was 34% in fish meat, 26.8% in  poultry meat and 2% in minced meat (beef and pork). All investigated A. butzleri  isolates carried the genes cadF, ciaB, cj1349, mviN and pldA. The gene tlyA was  detectable in 97.5% of the strains. Lower detection rates were observed for hecA  (47.5%), hecB (45%), iroE (40%) and irgA (35%). Genotyping by ERIC-PCR demonstrated  a high genetic diversity of A. butzleri strains from different foods. In  conclusion, this study shows that about one third of fish meat and poultry meat  samples contained Arcobacter spp. These data highlight the need to strengthen  our effort to elucidate the importance of Arcobacter on veterinary public health.","date":{"year":2014,"date":"12\/2014","accepted":"2014-12-22"},"volume":"128","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"163-168","redirects":["prevalence-virulence-gene-distribution-and-genetic-diversity-of-arcobacter-in-food-samples-in-germany\/150\/3216\/84358"],"tierartCategories":[],"artikelartCategories":["Open Access","Abostufe frei"]}
%8 12/2014