TY - JOUR
KW - small ruminant
KW - New World camelids
KW - pseudotuberculosis
KW - Actinomyces
KW - Actinomycetes
KW - Actinomycetaceae
KW - Schaalia
AU - R Sting
AU - A Schwalm
AU - M Contzen
AU - M Roller
AU - J Rau
AB - The genera Actinomyces and Schaalia, both members of the bacterial order Actinomycetales, include relevant infectious agents that cause abscesses in small ruminants and New World camelids. Due to the high diversity of the Actinomycetales, detection of undescribed members of this order is to be expected. Novel actinomycetes species were cultivated from a goat, a llama and two alpacas suffering from abscesses with suspected caseous lymphadenitis (CLA). Analyses carried out on these isolates using MALDI-TOF MS and 16S rRNA gene sequencing revealed actinomycetes, presumably belonging to the bacterial genera Actinomyces and Schaalia. The data suggest that the caprine isolate is a undescribed Actinomyces species, while the isolates originating from a llama and two alpacas show a close relationship to each other within a unique Schaalia cluster, suggesting a host-adapted novel Schaalia species. Both methods proved equally suitable for reliable identification of known and of undescribed Actinomyces and Schaalia species. This study contributes to extending our knowledge about novel species belonging to the bacterial family of Actinomycetaceae (actinomycetes) associated with abscesses in goats and New World camelids. Precise identification of actinomycetes at species level is of high relevance in veterinary practice with regard to differentiation from caseous lymphadenitis and assessment of treatment success.
BT - Berliner und Münchener Tierärztliche Wochenschrift
CY - Hannover
DA - 07/2020
DO - 10.2376/1439-0299-2020-6
LA - Englisch
N2 - The genera Actinomyces and Schaalia, both members of the bacterial order Actinomycetales, include relevant infectious agents that cause abscesses in small ruminants and New World camelids. Due to the high diversity of the Actinomycetales, detection of undescribed members of this order is to be expected. Novel actinomycetes species were cultivated from a goat, a llama and two alpacas suffering from abscesses with suspected caseous lymphadenitis (CLA). Analyses carried out on these isolates using MALDI-TOF MS and 16S rRNA gene sequencing revealed actinomycetes, presumably belonging to the bacterial genera Actinomyces and Schaalia. The data suggest that the caprine isolate is a undescribed Actinomyces species, while the isolates originating from a llama and two alpacas show a close relationship to each other within a unique Schaalia cluster, suggesting a host-adapted novel Schaalia species. Both methods proved equally suitable for reliable identification of known and of undescribed Actinomyces and Schaalia species. This study contributes to extending our knowledge about novel species belonging to the bacterial family of Actinomycetaceae (actinomycetes) associated with abscesses in goats and New World camelids. Precise identification of actinomycetes at species level is of high relevance in veterinary practice with regard to differentiation from caseous lymphadenitis and assessment of treatment success.
PB - Schlütersche Verlagsgesellschaft mbH & Co. KG
PP - Hannover
PY - 2020
T1 - Actinomycetes associated with abscess formation in a goat, a llama and two alpacas 
T2 - Berliner und Münchener Tierärztliche Wochenschrift
TI - Actinomycetes associated with abscess formation in a goat, a llama and two alpacas 
TT - Actinomyceten im Zusammenhang mit Abszessen bei  einer Ziege, einem Lama und zwei Alpakas 
VL - 133
SN - 1439-0299
ER -