TY - JOUR
KW - 16S rRNA gene
KW - 16S-23S rDNA intergenic spacer region ISR
KW - gene tuf
KW - rpoB
KW - pyolysin
AU - M Alssahen
AU - M Peters
AU - J Rau
AU - A Hassan
AU - O Sammra
AU - C Lämmler
AU - E Prenger-Berninghoff
AU - M Plötz
AU - A Abdulmawjood
AB - In the present study, a single Trueperella (T.) pyogenes strain isolated from a pneumonic lung of an Eurasian lynx (Lynx lynx) was identified and further characterized by phenotypical investigations, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), by Fourier Transform Infrared Spectroscopy (FT-IR) and genotypically by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the elongation factor tu encoding gene tuf, the β-subunit of bacterial RNA polymerase encoding gene rpoB and by sequencing the virulence factor pyolysin encoding gene plo. 
The present study gives a detailed characterization of a single strain of the species T. pyogenes from an Eurasian lynx. However, the route of infection of the Eurasian lynx with the bacterial pathogen remains unclear.
BT - Berliner und Münchener Tierärztliche Wochenschrift
CY - Hannover
DA - 03/2020
DO - 10.2376/0005-9366-19037
LA - Englisch
N2 - In the present study, a single Trueperella (T.) pyogenes strain isolated from a pneumonic lung of an Eurasian lynx (Lynx lynx) was identified and further characterized by phenotypical investigations, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), by Fourier Transform Infrared Spectroscopy (FT-IR) and genotypically by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the elongation factor tu encoding gene tuf, the β-subunit of bacterial RNA polymerase encoding gene rpoB and by sequencing the virulence factor pyolysin encoding gene plo. 
The present study gives a detailed characterization of a single strain of the species T. pyogenes from an Eurasian lynx. However, the route of infection of the Eurasian lynx with the bacterial pathogen remains unclear.
PB - Schlütersche Verlagsgesellschaft mbH & Co. KG
PP - Hannover
PY - 2020
T1 - Phenotypic and genotypic approach  to characterize a Trueperella pyogenes strain isolated from an Eurasian lynx (Lynx lynx)
T2 - Berliner und Münchener Tierärztliche Wochenschrift
TI - Phenotypic and genotypic approach  to characterize a Trueperella pyogenes strain isolated from an Eurasian lynx (Lynx lynx)
TT - Phänotypische und genotypische Charakterisierung eines Trueperella pyogenes-Stamms, isoliert von einem Eurasischen Luchs (Lynx lynx)
VL - 133
SN - 0005-9366
ER -