TY - JOUR
KW - Trueperella pyogenes
KW - MALDI-TOF MS
KW - dairy cattle
KW - Species-specific PCR
KW - molecular identification
AU - S Nagib
AU - M Timke
AU - C Lämmler
AU - J Spergser
AU - J Aurich
AB - Trueperella (T.) pyogenes is a worldwide causative agent of purulent-abscessing inflammatory processes, especially in cattle and pigs. T. pyogenes has also been found in clinically healthy cows and even in the gravid uterus. The aim of the present study was to identify a significant difference between two groups of T. pyogenes isolated from diseased and apparently healthy animals through investigating putative virulence factor-encoding and MALDI-TOF MS analysis. In the present study, T. pyogenes (n=14) isolated from the genital tract of endometritic (n=8) and apparently healthy dairy cattle (n=6) together with 12 reference strains representing ten species of the genera Trueperella and Arcanobacterium were characterised phenotypically, by MALDI-TOF MS analysis and genotypically by investigating the molecular targets ISR und sodA. The further genotypic methods included amplification of the putative virulence factor-encoding genes plo, cbpA, nanH, nanP, fimA, fimC, fimE and tet(W). The distribution of the virulence factor encoding genes of the T. pyogenes revealed no significant differences between the two groups, indicating that additional criteria might be responsible for the onset and aetiopathology of bovine endometritis. 
BT - Berliner und Münchener Tierärztliche Wochenschrift
CY - Hannover
DA - 04/2020
DO - 10.2376/0005-9366-19025
LA - Englisch
N2 - Trueperella (T.) pyogenes is a worldwide causative agent of purulent-abscessing inflammatory processes, especially in cattle and pigs. T. pyogenes has also been found in clinically healthy cows and even in the gravid uterus. The aim of the present study was to identify a significant difference between two groups of T. pyogenes isolated from diseased and apparently healthy animals through investigating putative virulence factor-encoding and MALDI-TOF MS analysis. In the present study, T. pyogenes (n=14) isolated from the genital tract of endometritic (n=8) and apparently healthy dairy cattle (n=6) together with 12 reference strains representing ten species of the genera Trueperella and Arcanobacterium were characterised phenotypically, by MALDI-TOF MS analysis and genotypically by investigating the molecular targets ISR und sodA. The further genotypic methods included amplification of the putative virulence factor-encoding genes plo, cbpA, nanH, nanP, fimA, fimC, fimE and tet(W). The distribution of the virulence factor encoding genes of the T. pyogenes revealed no significant differences between the two groups, indicating that additional criteria might be responsible for the onset and aetiopathology of bovine endometritis. 
PB - Schlütersche Verlagsgesellschaft mbH & Co. KG
PP - Hannover
PY - 2020
T1 - Genomic characterisation of Trueperella pyogenes isolates from the genital tract of healthy and endometritic dairy cattle
T2 - Berliner und Münchener Tierärztliche Wochenschrift
TI - Genomic characterisation of Trueperella pyogenes isolates from the genital tract of healthy and endometritic dairy cattle
TT - Genomische Charakterisierung von Trueperella pyogenes-Isolaten aus dem Genitaltrakt von gesunden und an Endometritis erkrankten Milchkühen
VL - 133
SN - 0005-9366
ER -