TY - JOUR KW - PCR KW - RFLP KW - primer KW - animals KW - diagnostics AU - Z Sihelská AU - T Csank AU - E Čonková AU - M Harčárová AU - P Váczi AB - The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia species in mammals. A new PCR-RFLP method with primers MalasF (5´-GAGCATGCCTGTTTGAGTGCC-3´) and MalasR (5´-GCAAATGACGTATCATGCCAT-3´) and with the enzymes MspI, EcoRI and PflMI was especially developed for the detection of nine animal Malassezia species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi and M. nana). The method is technically simple and capable of identifying Malassezia species of animals with high accuracy and reliability. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":105561,"title":"PCR-RFLP for identification of animal Malassezia species in mammals","topline":"","teaserText":"PCR-RFLP zur Identifizierung von tierischen Malassezia-Arten bei S\u00e4ugetieren","content":"

Summary<\/span>
The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia<\/span> is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia<\/span> species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia<\/span> species in mammals. A new PCR-RFLP method with primers MalasF (5\u00b4-GAGCATGCCTGTTTGAGTGCC-3\u00b4) and MalasR (5\u00b4-GCAAATGACGTATCATGCCAT-3\u00b4) and with the enzymes MspI<\/span>, Eco<\/span>RI and Pf<\/span>lMI was especially developed for the detection of nine animal Malassezia<\/span> species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi<\/span> and M. nana<\/span>). The method is technically simple and capable of identifying Malassezia<\/span> species of animals with high accuracy and reliability.<\/p>

Keywords<\/span>
PCR, RFLP, primer, animals, diagnostics <\/p>

Zusammenfassung<\/span>
Die Gattung Malassezia<\/span> umfasst 16 Arten, von denen elf Arten bei Tieren vorkommen (9 Arten bei S\u00e4ugetieren und 2 Arten bei V\u00f6geln). Die Diagnostik von Malassezia<\/span> basiert auf der ph\u00e4notypischen und genotypischen Identifikation. Die ph\u00e4notypische Untersuchung ist nicht ausreichend empfindlich und exakt und daher sind die molekularen Methoden f\u00fcr eine genaue Identifizierung notwendig. F\u00fcr die Diagnostik von Malassezia<\/span>-Spezies wurde eine Reihe von molekularen Methoden entwickelt, aber es gibt mehrere mit ihnen verbundene Nachteile, wie die Ineffizienz der Differenzierung aller Arten, technische Schwierigkeiten, hohe Kosten und die fragw\u00fcrdige Reproduzierbarkeit. Es ist keine einfache Methode verf\u00fcgbar, die die Identifizierung aller Malassezia<\/span>-Arten bei S\u00e4ugetieren erm\u00f6glicht. Eine neue PCR-RFLP-Methode mit Primern MalasF (5\u2018-GAGCATGCCTGTTTGAGTGCC -3\u2018) und MalasR (5\u2018-GCAAATGACGTATCATGCCAT-3\u2018) und mit den Enzymen MspI<\/span>, Eco<\/span>RI und Pf<\/span>lMI wurde speziell f\u00fcr den Nachweis von neun Malassezia<\/span>-Spezies in S\u00e4ugetieren entwickelt (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis M. caprae, M. equina, M. cuniculi<\/span> und M. nana<\/span>). Die Methode ist technisch einfach und in der Lage, Malassezia<\/span>-Arten von Tieren mit hoher Genauigkeit und Zuverl\u00e4ssigkeit zu identifizieren.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
CR, RFLP, Primer, Tiere, Diagnostik <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Nov 29, 2017 8:04:14 AM","oldUrls":["http:\/\/vetline.de\/pcr-rflp-for-identification-of-animal-malassezia-species-in-mammals\/150\/3130\/105561"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiDocumentUri":"https:\/\/vetline.de\/files\/smfiledata\/6\/9\/2\/2\/6\/2\/BMW_AOP_17035_Sihelska_onl300_ges.pdf","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-17035","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"Sihelsk\u00e1 Z, Csank T, \u010conkov\u00e1 E, Har\u010d\u00e1rov\u00e1 M, V\u00e1czi P","pdf":{"path":"http:\/\/data\/BMW_AOP_17035_Sihelska_onl300_ges.pdf","title":"BMW AOP 17035 Sihelsk\u00e1","description":"PCR-RFLP for identification of animal Malassezia species in mammals"},"authors":[{"firstName":"Z","middleName":"","lastName":"Sihelsk\u00e1"},{"firstName":"T","middleName":"","lastName":"Csank"},{"firstName":"E","middleName":"","lastName":"\u010conkov\u00e1"},{"firstName":"M","middleName":"","lastName":"Har\u010d\u00e1rov\u00e1"},{"firstName":"P","middleName":"","lastName":"V\u00e1czi"}],"contentOptimised":"

Summary<\/strong>
The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia<\/em> is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia<\/em> species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia<\/em> species in mammals. A new PCR-RFLP method with primers MalasF (5\u00b4-GAGCATGCCTGTTTGAGTGCC-3\u00b4) and MalasR (5\u00b4-GCAAATGACGTATCATGCCAT-3\u00b4) and with the enzymes MspI<\/em>, Eco<\/em>RI and Pf<\/em>lMI was especially developed for the detection of nine animal Malassezia<\/em> species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi<\/em> and M. nana<\/em>). The method is technically simple and capable of identifying Malassezia<\/em> species of animals with high accuracy and reliability.<\/p>

Keywords:<\/strong>
PCR, RFLP, primer, animals, diagnostics <\/p>

Zusammenfassung<\/strong>
Die Gattung Malassezia<\/em> umfasst 16 Arten, von denen elf Arten bei Tieren vorkommen (9 Arten bei S\u00e4ugetieren und 2 Arten bei V\u00f6geln). Die Diagnostik von Malassezia<\/em> basiert auf der ph\u00e4notypischen und genotypischen Identifikation. Die ph\u00e4notypische Untersuchung ist nicht ausreichend empfindlich und exakt und daher sind die molekularen Methoden f\u00fcr eine genaue Identifizierung notwendig. F\u00fcr die Diagnostik von Malassezia<\/em>-Spezies wurde eine Reihe von molekularen Methoden entwickelt, aber es gibt mehrere mit ihnen verbundene Nachteile, wie die Ineffizienz der Differenzierung aller Arten, technische Schwierigkeiten, hohe Kosten und die fragw\u00fcrdige Reproduzierbarkeit. Es ist keine einfache Methode verf\u00fcgbar, die die Identifizierung aller Malassezia<\/em>-Arten bei S\u00e4ugetieren erm\u00f6glicht. Eine neue PCR-RFLP-Methode mit Primern MalasF (5\u2018-GAGCATGCCTGTTTGAGTGCC -3\u2018) und MalasR (5\u2018-GCAAATGACGTATCATGCCAT-3\u2018) und mit den Enzymen MspI<\/em>, Eco<\/em>RI und Pf<\/em>lMI wurde speziell f\u00fcr den Nachweis von neun Malassezia<\/em>-Spezies in S\u00e4ugetieren entwickelt (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis M. caprae, M. equina, M. cuniculi<\/em> und M. nana<\/em>). Die Methode ist technisch einfach und in der Lage, Malassezia<\/em>-Arten von Tieren mit hoher Genauigkeit und Zuverl\u00e4ssigkeit zu identifizieren.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
CR, RFLP, Primer, Tiere, Diagnostik <\/p>","primaryLanguage":"englisch","summary":"The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia<\/em> is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia<\/em> species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia<\/em> species in mammals. A new PCR-RFLP method with primers MalasF (5\u00b4-GAGCATGCCTGTTTGAGTGCC-3\u00b4) and MalasR (5\u00b4-GCAAATGACGTATCATGCCAT-3\u00b4) and with the enzymes MspI<\/em>, Eco<\/em>RI and Pf<\/em>lMI was especially developed for the detection of nine animal Malassezia<\/em> species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi<\/em> and M. nana<\/em>). The method is technically simple and capable of identifying Malassezia<\/em> species of animals with high accuracy and reliability.<\/p>

","keywords":["PCR","RFLP","primer","animals","diagnostics"],"zusammenfassung":"Die Gattung Malassezia<\/em> umfasst 16 Arten, von denen elf Arten bei Tieren vorkommen (9 Arten bei S\u00e4ugetieren und 2 Arten bei V\u00f6geln). Die Diagnostik von Malassezia<\/em> basiert auf der ph\u00e4notypischen und genotypischen Identifikation. Die ph\u00e4notypische Untersuchung ist nicht ausreichend empfindlich und exakt und daher sind die molekularen Methoden f\u00fcr eine genaue Identifizierung notwendig. F\u00fcr die Diagnostik von Malassezia<\/em>-Spezies wurde eine Reihe von molekularen Methoden entwickelt, aber es gibt mehrere mit ihnen verbundene Nachteile, wie die Ineffizienz der Differenzierung aller Arten, technische Schwierigkeiten, hohe Kosten und die fragw\u00fcrdige Reproduzierbarkeit. Es ist keine einfache Methode verf\u00fcgbar, die die Identifizierung aller Malassezia<\/em>-Arten bei S\u00e4ugetieren erm\u00f6glicht. Eine neue PCR-RFLP-Methode mit Primern MalasF (5\u2018-GAGCATGCCTGTTTGAGTGCC -3\u2018) und MalasR (5\u2018-GCAAATGACGTATCATGCCAT-3\u2018) und mit den Enzymen MspI<\/em>, Eco<\/em>RI und Pf<\/em>lMI wurde speziell f\u00fcr den Nachweis von neun Malassezia<\/em>-Spezies in S\u00e4ugetieren entwickelt (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis M. caprae, M. equina, M. cuniculi<\/em> und M. nana<\/em>). Die Methode ist technisch einfach und in der Lage, Malassezia<\/em>-Arten von Tieren mit hoher Genauigkeit und Zuverl\u00e4ssigkeit zu identifizieren.<\/p>

","schluesselwoerter":["CR","RFLP","Primer","Tiere","Diagnostik"],"translatedTitle":"PCR-RFLP zur Identifizierung von tierischen Malassezia-Arten bei S\u00e4ugetieren","abstractE":"The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia species in mammals. A new PCR-RFLP method with primers MalasF (5\u00b4-GAGCATGCCTGTTTGAGTGCC-3\u00b4) and MalasR (5\u00b4-GCAAATGACGTATCATGCCAT-3\u00b4) and with the enzymes MspI, EcoRI and PflMI was especially developed for the detection of nine animal Malassezia species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi and M. nana). The method is technically simple and capable of identifying Malassezia species of animals with high accuracy and reliability.","date":{"year":2017,"date":"11\/2017","accepted":"2017-11-29"},"volume":130,"openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["pcr-rflp-for-identification-of-animal-malassezia-species-in-mammals\/150\/3130\/105561"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 11/2017 DO - 10.2376/0005-9366-17035 LA - English N2 - The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia species in mammals. A new PCR-RFLP method with primers MalasF (5´-GAGCATGCCTGTTTGAGTGCC-3´) and MalasR (5´-GCAAATGACGTATCATGCCAT-3´) and with the enzymes MspI, EcoRI and PflMI was especially developed for the detection of nine animal Malassezia species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi and M. nana). The method is technically simple and capable of identifying Malassezia species of animals with high accuracy and reliability. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 T1 - PCR-RFLP for identification of animal Malassezia species in mammals T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - PCR-RFLP for identification of animal Malassezia species in mammals TT - PCR-RFLP zur Identifizierung von tierischen Malassezia-Arten bei Säugetieren VL - 130 SN - 0005-9366 ER -