TY - JOUR KW - MALDI-TOF MS KW - dairy cattle KW - Species-specific PCR KW - molecular identification KW - virulence gene AU - S Nagib AU - O Sammra AU - C Lämmler AU - A Abdulmawjood AU - R Huber-Schlenstedt AU - M Timke AU - M Kostrzewa AU - E Prenger-Berninghoff AB - In the present study, we identified and characterized a single Trueperella (T.) pyogenes strain 754B isolated from dairy milk using several phenotypical methods, MALDI-TOF MS fingerprinting and by various genotypic techniques investigating the molecular target genes 16S rRNA, ISR, sodA and gap. The genotypic techniques also included the amplification of the putative virulence factor encoding genes plo, cbpA, nanH, nanP, fimA, fimC, fimE and tet(W) specific for T. pyogenes. Screening of the presence of the putative virulence genes showed that the T. pyogenes 754B possess genes plo, fimA, fimC, fimE and tet(W). Meanwhile, the investigated isolate was negative for the presence of gene cbpA and both neuraminidases encoding genes nanH and nanP. However, it was of interest that T. pyogenes 754B was lacking any extracellular proteolytic activity which is generally typical for T. pyogenes and is widely used for phenotypic identification of this species. We consider this study as the first observation of T. pyogenes with a total lack of proteolytic activity. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":105429,"title":"Identification and characterization of a Trueperella pyogenes deficient in proteolytic activity isolated from bovine mastitis: a case report","topline":"","teaserText":"Identifizierung und Charakterisierung eines Trueperella pyogenes-Isolates ohne proteolytische Aktivit\u00e4t, isoliert aus Kuhmilch mit Mastitshintergrund: ein Fallbericht","content":"

Summary<\/span>
In the present study, we identified and characterized a single Trueperella<\/span> (T<\/span>.) pyogenes<\/span> strain 754B isolated from dairy milk using several phenotypical methods, MALDI-TOF MS fingerprinting and by various genotypic techniques investigating the molecular target genes 16S rRNA, ISR, sod<\/span>A<\/span> and gap<\/span>. The genotypic techniques also included the amplification of the putative virulence factor encoding genes plo, <\/span>cbpA, nanH, nanP, fimA, fimC, fimE<\/span> and tet<\/span>(W<\/span>) specific for T. pyogenes<\/span>. Screening of the presence of the putative virulence genes showed that the T. pyogenes<\/span> 754B possess genes plo, fimA, fimC, fimE<\/span> and tet<\/span>(W<\/span>). Meanwhile, the investigated isolate was negative for the presence of gene cbpA and both neuraminidases encoding genes nanH<\/span> and nanP<\/span>. However, it was of interest that T. pyogenes<\/span> 754B was lacking any extracellular proteolytic activity which is generally typical for T. pyogenes<\/span> and is widely used for phenotypic identification of this species. We consider this study as the first observation of T. pyogenes<\/span> with a total lack of proteolytic activity.<\/p>

Keywords<\/span>
MALDI-TOF MS, dairy cattle, Species-specific PCR, molecular identification, virulence gene<\/p>

Zusammenfassung<\/span>
In der vorliegenden Studie wurde das Trueperella pyogenes<\/span>-Isolat 754B durch ph\u00e4notypische Methoden, mittels der MALDI-TOF MS-Technik und durch verschiedene genotypische Techniken durch Nachweis der molekularen Zielstrukturen 16S rRNA, ISR, sodA<\/span> und gap<\/span> untersucht und identifiziert. Das Isolat stammte aus Milch einer an Mastitis erkrankten Kuh. Die genotypischen Methoden enthielten des Weiteren die Amplifizierung der mutma\u00dflichen Virulenzfaktor-kodierenden Gene plo<\/span>, cbpA, nanH, nanP, fimA, fimC, fimE<\/span> und tet<\/span>(W<\/span>). Folgende Virulenzfaktor-kodierenden Gene wurden nachgewiesen: das Pyolysin-kodierende Gen plo<\/span>, die Fimbrien-Untereinheiten kodierenden Gene fimA, fimC, fimE<\/span> und das Tetracyclin-Resistenzgen tet<\/span>(W<\/span>). Das Gen cbpA<\/span> des kollagenbindenden Proteins CbpA und die Gene nanH<\/span> und nanP<\/span>, die die Neuraminidasen NanH und NanP<\/span> kodieren, wurden untersucht, aber nicht detektiert. Vertreter der Spezies T. pyogenes<\/span> weisen typischerweise eine extrazellul\u00e4re proteolytische Aktivit\u00e4t auf, die auch f\u00fcr die ph\u00e4notypische Identifikation der Spezies verwendet wird. Mit Isolat 754B erfolgte zum ersten Mal eine ph\u00e4notypische und genotypische Charakterisierung eines T. pyogenes<\/span>-Isolates ohne proteolytische Aktivit\u00e4t.
<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
MALDI-TOF MS, Milchk\u00fche, Spezies-spezifische PCR, molekulare Identifizierung, Virulenzgene<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Nov 21, 2017 10:06:39 AM","oldUrls":["http:\/\/vetline.de\/identification-and-characterization-of-a-trueperella-pyogenes-deficient-in-proteolytic-activity-isolated-from-bovine-mastitis-a-case-report\/150\/3130\/105429"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiDocumentUri":"https:\/\/vetline.de\/files\/smfiledata\/6\/9\/0\/9\/9\/4\/BMW_AOP_17028_Nagib_onl300_ges.pdf","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-17028","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"Nagib S, Sammra O, L\u00e4mmler C, Abdulmawjood A, Huber-Schlenstedt R, Timke M, Kostrzewa M, Prenger-Berninghoff E","pdf":{"path":"http:\/\/data\/BMW_AOP_17028_Nagib_onl300_ges.pdf","title":"BMW AOP 17028 Nagib","description":"Identification and characterization of a Trueperella pyogenes deficient in\r\nproteolytic activity isolated from bovine mastitis: a case report"},"authors":[{"firstName":"S","middleName":"","lastName":"Nagib"},{"firstName":"O","middleName":"","lastName":"Sammra"},{"firstName":"C","middleName":"","lastName":"L\u00e4mmler"},{"firstName":"A","middleName":"","lastName":"Abdulmawjood"},{"firstName":"R","middleName":"","lastName":"Huber-Schlenstedt"},{"firstName":"M","middleName":"","lastName":"Timke"},{"firstName":"M","middleName":"","lastName":"Kostrzewa"},{"firstName":"E","middleName":"","lastName":"Prenger-Berninghoff"}],"contentOptimised":"

Summary<\/strong>
In the present study, we identified and characterized a single Trueperella<\/em> (T<\/em>.) pyogenes<\/em> strain 754B isolated from dairy milk using several phenotypical methods, MALDI-TOF MS fingerprinting and by various genotypic techniques investigating the molecular target genes 16S rRNA, ISR, sod<\/em>A<\/em> and gap<\/em>. The genotypic techniques also included the amplification of the putative virulence factor encoding genes plo, <\/em>cbpA, nanH, nanP, fimA, fimC, fimE<\/em> and tet<\/em>(W<\/em>) specific for T. pyogenes<\/em>. Screening of the presence of the putative virulence genes showed that the T. pyogenes<\/em> 754B possess genes plo, fimA, fimC, fimE<\/em> and tet<\/em>(W<\/em>). Meanwhile, the investigated isolate was negative for the presence of gene cbpA and both neuraminidases encoding genes nanH<\/em> and nanP<\/em>. However, it was of interest that T. pyogenes<\/em> 754B was lacking any extracellular proteolytic activity which is generally typical for T. pyogenes<\/em> and is widely used for phenotypic identification of this species. We consider this study as the first observation of T. pyogenes<\/em> with a total lack of proteolytic activity.<\/p>

Keywords:<\/strong>
MALDI-TOF MS, dairy cattle, Species-specific PCR, molecular identification, virulence gene<\/p>

Zusammenfassung<\/strong>
In der vorliegenden Studie wurde das Trueperella pyogenes<\/em>-Isolat 754B durch ph\u00e4notypische Methoden, mittels der MALDI-TOF MS-Technik und durch verschiedene genotypische Techniken durch Nachweis der molekularen Zielstrukturen 16S rRNA, ISR, sodA<\/em> und gap<\/em> untersucht und identifiziert. Das Isolat stammte aus Milch einer an Mastitis erkrankten Kuh. Die genotypischen Methoden enthielten des Weiteren die Amplifizierung der mutma\u00dflichen Virulenzfaktor-kodierenden Gene plo<\/em>, cbpA, nanH, nanP, fimA, fimC, fimE<\/em> und tet<\/em>(W<\/em>). Folgende Virulenzfaktor-kodierenden Gene wurden nachgewiesen: das Pyolysin-kodierende Gen plo<\/em>, die Fimbrien-Untereinheiten kodierenden Gene fimA, fimC, fimE<\/em> und das Tetracyclin-Resistenzgen tet<\/em>(W<\/em>). Das Gen cbpA<\/em> des kollagenbindenden Proteins CbpA und die Gene nanH<\/em> und nanP<\/em>, die die Neuraminidasen NanH und NanP<\/em> kodieren, wurden untersucht, aber nicht detektiert. Vertreter der Spezies T. pyogenes<\/em> weisen typischerweise eine extrazellul\u00e4re proteolytische Aktivit\u00e4t auf, die auch f\u00fcr die ph\u00e4notypische Identifikation der Spezies verwendet wird. Mit Isolat 754B erfolgte zum ersten Mal eine ph\u00e4notypische und genotypische Charakterisierung eines T. pyogenes<\/em>-Isolates ohne proteolytische Aktivit\u00e4t.
<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
MALDI-TOF MS, Milchk\u00fche, Spezies-spezifische PCR, molekulare Identifizierung, Virulenzgene<\/p>","primaryLanguage":"englisch","summary":"In the present study, we identified and characterized a single Trueperella<\/em> (T<\/em>.) pyogenes<\/em> strain 754B isolated from dairy milk using several phenotypical methods, MALDI-TOF MS fingerprinting and by various genotypic techniques investigating the molecular target genes 16S rRNA, ISR, sod<\/em>A<\/em> and gap<\/em>. The genotypic techniques also included the amplification of the putative virulence factor encoding genes plo, <\/em>cbpA, nanH, nanP, fimA, fimC, fimE<\/em> and tet<\/em>(W<\/em>) specific for T. pyogenes<\/em>. Screening of the presence of the putative virulence genes showed that the T. pyogenes<\/em> 754B possess genes plo, fimA, fimC, fimE<\/em> and tet<\/em>(W<\/em>). Meanwhile, the investigated isolate was negative for the presence of gene cbpA and both neuraminidases encoding genes nanH<\/em> and nanP<\/em>. However, it was of interest that T. pyogenes<\/em> 754B was lacking any extracellular proteolytic activity which is generally typical for T. pyogenes<\/em> and is widely used for phenotypic identification of this species. We consider this study as the first observation of T. pyogenes<\/em> with a total lack of proteolytic activity.<\/p>

","keywords":["MALDI-TOF MS","dairy cattle","Species-specific PCR","molecular identification","virulence gene"],"zusammenfassung":"In der vorliegenden Studie wurde das Trueperella pyogenes<\/em>-Isolat 754B durch ph\u00e4notypische Methoden, mittels der MALDI-TOF MS-Technik und durch verschiedene genotypische Techniken durch Nachweis der molekularen Zielstrukturen 16S rRNA, ISR, sodA<\/em> und gap<\/em> untersucht und identifiziert. Das Isolat stammte aus Milch einer an Mastitis erkrankten Kuh. Die genotypischen Methoden enthielten des Weiteren die Amplifizierung der mutma\u00dflichen Virulenzfaktor-kodierenden Gene plo<\/em>, cbpA, nanH, nanP, fimA, fimC, fimE<\/em> und tet<\/em>(W<\/em>). Folgende Virulenzfaktor-kodierenden Gene wurden nachgewiesen: das Pyolysin-kodierende Gen plo<\/em>, die Fimbrien-Untereinheiten kodierenden Gene fimA, fimC, fimE<\/em> und das Tetracyclin-Resistenzgen tet<\/em>(W<\/em>). Das Gen cbpA<\/em> des kollagenbindenden Proteins CbpA und die Gene nanH<\/em> und nanP<\/em>, die die Neuraminidasen NanH und NanP<\/em> kodieren, wurden untersucht, aber nicht detektiert. Vertreter der Spezies T. pyogenes<\/em> weisen typischerweise eine extrazellul\u00e4re proteolytische Aktivit\u00e4t auf, die auch f\u00fcr die ph\u00e4notypische Identifikation der Spezies verwendet wird. Mit Isolat 754B erfolgte zum ersten Mal eine ph\u00e4notypische und genotypische Charakterisierung eines T. pyogenes<\/em>-Isolates ohne proteolytische Aktivit\u00e4t.
<\/p>

","schluesselwoerter":["MALDI-TOF MS","Milchk\u00fche","Spezies-spezifische PCR","molekulare Identifizierung","Virulenzgene"],"translatedTitle":"Identifizierung und Charakterisierung eines Trueperella pyogenes-Isolates ohne proteolytische Aktivit\u00e4t, isoliert aus Kuhmilch mit Mastitshintergrund: ein Fallbericht","abstractE":"In the present study, we identified and characterized a single Trueperella (T.) pyogenes strain 754B isolated from dairy milk using several phenotypical methods, MALDI-TOF MS fingerprinting and by various genotypic techniques investigating the molecular target genes 16S rRNA, ISR, sodA and gap. The genotypic techniques also included the amplification of the putative virulence factor encoding genes plo, cbpA, nanH, nanP, fimA, fimC, fimE and tet(W) specific for T. pyogenes. Screening of the presence of the putative virulence genes showed that the T. pyogenes 754B possess genes plo, fimA, fimC, fimE and tet(W). Meanwhile, the investigated isolate was negative for the presence of gene cbpA and both neuraminidases encoding genes nanH and nanP. However, it was of interest that T. pyogenes 754B was lacking any extracellular proteolytic activity which is generally typical for T. pyogenes and is widely used for phenotypic identification of this species. We consider this study as the first observation of T. pyogenes with a total lack of proteolytic activity.","date":{"year":2017,"date":"11\/2017","accepted":"2017-11-21"},"volume":130,"openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["identification-and-characterization-of-a-trueperella-pyogenes-deficient-in-proteolytic-activity-isolated-from-bovine-mastitis-a-case-report\/150\/3130\/105429"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 11/2017 DO - 10.2376/0005-9366-17028 LA - English N2 - In the present study, we identified and characterized a single Trueperella (T.) pyogenes strain 754B isolated from dairy milk using several phenotypical methods, MALDI-TOF MS fingerprinting and by various genotypic techniques investigating the molecular target genes 16S rRNA, ISR, sodA and gap. The genotypic techniques also included the amplification of the putative virulence factor encoding genes plo, cbpA, nanH, nanP, fimA, fimC, fimE and tet(W) specific for T. pyogenes. Screening of the presence of the putative virulence genes showed that the T. pyogenes 754B possess genes plo, fimA, fimC, fimE and tet(W). Meanwhile, the investigated isolate was negative for the presence of gene cbpA and both neuraminidases encoding genes nanH and nanP. However, it was of interest that T. pyogenes 754B was lacking any extracellular proteolytic activity which is generally typical for T. pyogenes and is widely used for phenotypic identification of this species. We consider this study as the first observation of T. pyogenes with a total lack of proteolytic activity. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 T1 - Identification and characterization of a Trueperella pyogenes deficient in proteolytic activity isolated from bovine mastitis: a case report T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Identification and characterization of a Trueperella pyogenes deficient in proteolytic activity isolated from bovine mastitis: a case report TT - Identifizierung und Charakterisierung eines Trueperella pyogenes-Isolates ohne proteolytische Aktivität, isoliert aus Kuhmilch mit Mastitshintergrund: ein Fallbericht VL - 130 SN - 0005-9366 ER -