TY - JOUR KW - caseous lymphadenitis KW - camelid KW - ELISA KW - MALDI-TOF MS KW - FT-IR AU - R Sting AU - W Rietschel AU - B Polley AU - C Süß-Dombrowski AU - J Rau AB - This is a report of a severe infection with Corynebacterium (C.) pseudotuberculosis in a dromedary (Camelus dromedarius) in Germany. The eight year old, female dromedary was integrated into a new herd in August 2014 for breeding purposes. At this time, several animals in the herd already suffered from nodes in the skin and in July 201, the newly introduced dromedary also developed multiple subcutaneous nodes and a large abscess on the lower abdomen. When returning home in early August 201, the dromedary was euthanised for reasons of animal welfare and due to detection of C. pseudotuberculosis and unsuccessful antibiotic treatment of the abscess and arthritis. Post mortem examination revealed multiple subcutaneous nodes and abscesses in the lung which were surrounded by a fibrous capsule and filled with yellowish, creamy pus. Histopathological examination showed a granulomatous inflammation. Bacteriological examination found strong growth of corynebacteria accompanied by modest growth of staphylococci and these could be clearly identified as C. pseudotuberculosis and Staphylococcus aureus using MALDI-TOF MS and FT-IR analyses. Serological investigations using an in-lab and a commercially available C. pseudotuberculosis ELISA yielded strong positive reactions. This case of severe caseous lymphadenitis in a dromedary clearly shows the high risk of introduction of infections if attention is not paid to individual and herd health status. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":105368,"title":"Corynebacterium pseudotuberculosis infection in a dromedary (Camelus dromedarius) in Germany","topline":"","teaserText":"Corynebacterium pseudotuberculosis-Infektion bei einem Dromedar (Camelus dromedarius) in Deutschland","content":"

Summary<\/span>
This is a report of a severe infection with Corynebacterium (C.) pseudotuberculosis in a dromedary (Camelus dromedarius) in Germany. The eight year old, female dromedary was integrated into a new herd in August 2014 for breeding purposes. At this time, several animals in the herd already suffered from nodes in the skin and in July 201\ufeff, the newly introduced dromedary also developed multiple subcutaneous nodes and a large abscess on the lower abdomen. When returning home in early August 201\ufeff, the dromedary was euthanised for reasons of animal welfare and due to detection of C. pseudotuberculosis and unsuccessful antibiotic treatment of the abscess and arthritis. Post mortem examination revealed multiple subcutaneous nodes and abscesses in the lung which were surrounded by a fibrous capsule and filled with yellowish, creamy pus. Histopathological examination showed a granulomatous inflammation. Bacteriological examination found strong growth of corynebacteria accompanied by modest growth of staphylococci and these could be clearly identified as C. pseudotuberculosis and Staphylococcus aureus using MALDI-TOF MS and FT-IR analyses. Serological investigations using an in-lab and a commercially available C. pseudotuberculosis ELISA yielded strong positive reactions. This case of severe caseous lymphadenitis in a dromedary clearly shows the high risk of introduction of infections if attention is not paid to individual and herd health status.<\/p>

Keywords<\/span>
Caseous lymphadenitis, camelid, ELISA, MALDI-TOF MS, FT-IR<\/p>

Zusammenfassung<\/span>
Dies ist ein Bericht \u00fcber einen Fall einer schweren Infektion mit Corynebacterium (C.) pseudotuberculosis bei einem Dromedar (Camelus dromedarius) in Deutschland. Das acht Jahre alte, weibliche Dromedar wurde im August 2014 zu Zuchtzwecken in eine neue Herde integriert. Zu dieser Zeit zeigten bereits mehrere Dromedare dieser Herde Knoten in der Haut und im Juli 201\ufeff entwickelten sich bei dem neu eingef\u00fchrten Dromedar ebenfalls multiple Knoten in der Haut und ein gro\u00dfer Abszess am unteren Abdomen. Als das Dromedar Anfang August 201\ufeff zur\u00fcckkehrte, musste es aus Tierschutzgr\u00fcnden und aufgrund des Nachweises von C. pseudotuberculosis sowie erfolgloser antibiotischer Behandlungen des Abszesses und einer Arthritis euthanasiert werden. Bei der Sektion des Tieres konnten multiple subkutane Knoten und Abszesse in der Lunge, die von einer fibr\u00f6sen Kapsel umgeben und mit gelblichem, cremigem Eiter gef\u00fcllt waren, festgestellt werden. Histologische Untersuchungen der Unterhautknoten und der Lungenabszesse zeigten das Bild einer granulomat\u00f6sen Entz\u00fcndung. Die bakteriologische Untersuchung ergab ein starkes Wachstum von Corynebakterien, begleitet von m\u00e4\u00dfigem StaphylokokkenWachstum. Durch weiterf\u00fchrende Untersuchungen mittels MALDI-TOF MS und FT-IR konnten die Keime als C. pseudotuberculosis und Staphylococcus aureus identifiziert werden. Serologische Untersuchungen mittels eines selbst entwickelten und eines kommerziellen C. pseudotuberculosis-ELISA ergaben starke Reaktionen. Dieser Fall einer schweren Pseudotuberkulose bei einem Dromedar zeigt deutlich das hohe Risiko der Einschleppung einer Infektion, wenn der Gesundheitsstatus einzelner Tiere oder der Herde unbeachtet bleibt.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Pseudotuberkulose, Kameliden, ELISA, MALDI-TOF MS, FT-IR<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Nov 14, 2017 11:00:00 PM","oldUrls":["http:\/\/vetline.de\/corynebacterium-pseudotuberculosis-infection-in-a-dromedary-camelus-dromedarius-in-germany\/150\/3130\/105368"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift ","doiDocumentUri":"http:\/\/www.vetline.de\/corynebacterium-pseudotuberculosis-infection-in-a-dromedary-camelus-dromedarius-in-germany\/150\/3130\/105368\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 130, Heft 11\/12 (2017), Seiten 511\u2013516","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16082","doiFirstPage":"511","doiLastPage":"516","doiTransmitted":true,"doiAuthor":"Sting R, Rietschel W, Polley B, S\u00fc\u00df-Dombrowski C, Rau J","pdf":{"path":"http:\/\/data\/BMW_2017_11_12_0511.pdf","title":"BMW_2017_11_12_0511.pdf","description":"Corynebacterium pseudotuberculosis infection in a dromedary (Camelus dromedarius) in Germany"},"authors":[{"firstName":"R","middleName":"","lastName":"Sting"},{"firstName":"W","middleName":"","lastName":"Rietschel"},{"firstName":"B","middleName":"","lastName":"Polley"},{"firstName":"C","middleName":"","lastName":"S\u00fc\u00df-Dombrowski"},{"firstName":"J","middleName":"","lastName":"Rau"}],"contentOptimised":"

Summary<\/strong>
This is a report of a severe infection with Corynebacterium (C.) pseudotuberculosis in a dromedary (Camelus dromedarius) in Germany. The eight year old, female dromedary was integrated into a new herd in August 2014 for breeding purposes. At this time, several animals in the herd already suffered from nodes in the skin and in July 201\ufeff, the newly introduced dromedary also developed multiple subcutaneous nodes and a large abscess on the lower abdomen. When returning home in early August 201\ufeff, the dromedary was euthanised for reasons of animal welfare and due to detection of C. pseudotuberculosis and unsuccessful antibiotic treatment of the abscess and arthritis. Post mortem examination revealed multiple subcutaneous nodes and abscesses in the lung which were surrounded by a fibrous capsule and filled with yellowish, creamy pus. Histopathological examination showed a granulomatous inflammation. Bacteriological examination found strong growth of corynebacteria accompanied by modest growth of staphylococci and these could be clearly identified as C. pseudotuberculosis and Staphylococcus aureus using MALDI-TOF MS and FT-IR analyses. Serological investigations using an in-lab and a commercially available C. pseudotuberculosis ELISA yielded strong positive reactions. This case of severe caseous lymphadenitis in a dromedary clearly shows the high risk of introduction of infections if attention is not paid to individual and herd health status.<\/p>

Keywords:<\/strong>
Caseous lymphadenitis, camelid, ELISA, MALDI-TOF MS, FT-IR<\/p>

Zusammenfassung<\/strong>
Dies ist ein Bericht \u00fcber einen Fall einer schweren Infektion mit Corynebacterium (C.) pseudotuberculosis bei einem Dromedar (Camelus dromedarius) in Deutschland. Das acht Jahre alte, weibliche Dromedar wurde im August 2014 zu Zuchtzwecken in eine neue Herde integriert. Zu dieser Zeit zeigten bereits mehrere Dromedare dieser Herde Knoten in der Haut und im Juli 201\ufeff entwickelten sich bei dem neu eingef\u00fchrten Dromedar ebenfalls multiple Knoten in der Haut und ein gro\u00dfer Abszess am unteren Abdomen. Als das Dromedar Anfang August 201\ufeff zur\u00fcckkehrte, musste es aus Tierschutzgr\u00fcnden und aufgrund des Nachweises von C. pseudotuberculosis sowie erfolgloser antibiotischer Behandlungen des Abszesses und einer Arthritis euthanasiert werden. Bei der Sektion des Tieres konnten multiple subkutane Knoten und Abszesse in der Lunge, die von einer fibr\u00f6sen Kapsel umgeben und mit gelblichem, cremigem Eiter gef\u00fcllt waren, festgestellt werden. Histologische Untersuchungen der Unterhautknoten und der Lungenabszesse zeigten das Bild einer granulomat\u00f6sen Entz\u00fcndung. Die bakteriologische Untersuchung ergab ein starkes Wachstum von Corynebakterien, begleitet von m\u00e4\u00dfigem StaphylokokkenWachstum. Durch weiterf\u00fchrende Untersuchungen mittels MALDI-TOF MS und FT-IR konnten die Keime als C. pseudotuberculosis und Staphylococcus aureus identifiziert werden. Serologische Untersuchungen mittels eines selbst entwickelten und eines kommerziellen C. pseudotuberculosis-ELISA ergaben starke Reaktionen. Dieser Fall einer schweren Pseudotuberkulose bei einem Dromedar zeigt deutlich das hohe Risiko der Einschleppung einer Infektion, wenn der Gesundheitsstatus einzelner Tiere oder der Herde unbeachtet bleibt.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Pseudotuberkulose, Kameliden, ELISA, MALDI-TOF MS, FT-IR<\/p>","primaryLanguage":"englisch","summary":"This is a report of a severe infection with Corynebacterium (C.) pseudotuberculosis in a dromedary (Camelus dromedarius) in Germany. The eight year old, female dromedary was integrated into a new herd in August 2014 for breeding purposes. At this time, several animals in the herd already suffered from nodes in the skin and in July 201\ufeff, the newly introduced dromedary also developed multiple subcutaneous nodes and a large abscess on the lower abdomen. When returning home in early August 201\ufeff, the dromedary was euthanised for reasons of animal welfare and due to detection of C. pseudotuberculosis and unsuccessful antibiotic treatment of the abscess and arthritis. Post mortem examination revealed multiple subcutaneous nodes and abscesses in the lung which were surrounded by a fibrous capsule and filled with yellowish, creamy pus. Histopathological examination showed a granulomatous inflammation. Bacteriological examination found strong growth of corynebacteria accompanied by modest growth of staphylococci and these could be clearly identified as C. pseudotuberculosis and Staphylococcus aureus using MALDI-TOF MS and FT-IR analyses. Serological investigations using an in-lab and a commercially available C. pseudotuberculosis ELISA yielded strong positive reactions. This case of severe caseous lymphadenitis in a dromedary clearly shows the high risk of introduction of infections if attention is not paid to individual and herd health status.<\/p>

","keywords":["Caseous lymphadenitis","camelid","ELISA","MALDI-TOF MS","FT-IR"],"zusammenfassung":"Dies ist ein Bericht \u00fcber einen Fall einer schweren Infektion mit Corynebacterium (C.) pseudotuberculosis bei einem Dromedar (Camelus dromedarius) in Deutschland. Das acht Jahre alte, weibliche Dromedar wurde im August 2014 zu Zuchtzwecken in eine neue Herde integriert. Zu dieser Zeit zeigten bereits mehrere Dromedare dieser Herde Knoten in der Haut und im Juli 201\ufeff entwickelten sich bei dem neu eingef\u00fchrten Dromedar ebenfalls multiple Knoten in der Haut und ein gro\u00dfer Abszess am unteren Abdomen. Als das Dromedar Anfang August 201\ufeff zur\u00fcckkehrte, musste es aus Tierschutzgr\u00fcnden und aufgrund des Nachweises von C. pseudotuberculosis sowie erfolgloser antibiotischer Behandlungen des Abszesses und einer Arthritis euthanasiert werden. Bei der Sektion des Tieres konnten multiple subkutane Knoten und Abszesse in der Lunge, die von einer fibr\u00f6sen Kapsel umgeben und mit gelblichem, cremigem Eiter gef\u00fcllt waren, festgestellt werden. Histologische Untersuchungen der Unterhautknoten und der Lungenabszesse zeigten das Bild einer granulomat\u00f6sen Entz\u00fcndung. Die bakteriologische Untersuchung ergab ein starkes Wachstum von Corynebakterien, begleitet von m\u00e4\u00dfigem StaphylokokkenWachstum. Durch weiterf\u00fchrende Untersuchungen mittels MALDI-TOF MS und FT-IR konnten die Keime als C. pseudotuberculosis und Staphylococcus aureus identifiziert werden. Serologische Untersuchungen mittels eines selbst entwickelten und eines kommerziellen C. pseudotuberculosis-ELISA ergaben starke Reaktionen. Dieser Fall einer schweren Pseudotuberkulose bei einem Dromedar zeigt deutlich das hohe Risiko der Einschleppung einer Infektion, wenn der Gesundheitsstatus einzelner Tiere oder der Herde unbeachtet bleibt.<\/p>

","schluesselwoerter":["Pseudotuberkulose","Kameliden","ELISA","MALDI-TOF MS","FT-IR"],"translatedTitle":"Corynebacterium pseudotuberculosis-Infektion bei einem Dromedar (Camelus dromedarius) in Deutschland","abstractE":"This is a report of a severe infection with Corynebacterium (C.) pseudotuberculosis in a dromedary (Camelus dromedarius) in Germany. The eight year old, female dromedary was integrated into a new herd in August 2014 for breeding purposes. At this time, several animals in the herd already suffered from nodes in the skin and in July 201\ufeff, the newly introduced dromedary also developed multiple subcutaneous nodes and a large abscess on the lower abdomen. When returning home in early August 201\ufeff, the dromedary was euthanised for reasons of animal welfare and due to detection of C. pseudotuberculosis and unsuccessful antibiotic treatment of the abscess and arthritis. Post mortem examination revealed multiple subcutaneous nodes and abscesses in the lung which were surrounded by a fibrous capsule and filled with yellowish, creamy pus. Histopathological examination showed a granulomatous inflammation. Bacteriological examination found strong growth of corynebacteria accompanied by modest growth of staphylococci and these could be clearly identified as C. pseudotuberculosis and Staphylococcus aureus using MALDI-TOF MS and FT-IR analyses. Serological investigations using an in-lab and a commercially available C. pseudotuberculosis ELISA yielded strong positive reactions. This case of severe caseous lymphadenitis in a dromedary clearly shows the high risk of introduction of infections if attention is not paid to individual and herd health status.","date":{"year":2017,"date":"11\/2017","accepted":"2017-11-14"},"volume":"130","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"511-516","redirects":["corynebacterium-pseudotuberculosis-infection-in-a-dromedary-camelus-dromedarius-in-germany\/150\/3130\/105368"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 11/2017 DO - 10.2376/0005-9366-16082 ET - 11/12 LA - English N2 - This is a report of a severe infection with Corynebacterium (C.) pseudotuberculosis in a dromedary (Camelus dromedarius) in Germany. The eight year old, female dromedary was integrated into a new herd in August 2014 for breeding purposes. At this time, several animals in the herd already suffered from nodes in the skin and in July 201, the newly introduced dromedary also developed multiple subcutaneous nodes and a large abscess on the lower abdomen. When returning home in early August 201, the dromedary was euthanised for reasons of animal welfare and due to detection of C. pseudotuberculosis and unsuccessful antibiotic treatment of the abscess and arthritis. Post mortem examination revealed multiple subcutaneous nodes and abscesses in the lung which were surrounded by a fibrous capsule and filled with yellowish, creamy pus. Histopathological examination showed a granulomatous inflammation. Bacteriological examination found strong growth of corynebacteria accompanied by modest growth of staphylococci and these could be clearly identified as C. pseudotuberculosis and Staphylococcus aureus using MALDI-TOF MS and FT-IR analyses. Serological investigations using an in-lab and a commercially available C. pseudotuberculosis ELISA yielded strong positive reactions. This case of severe caseous lymphadenitis in a dromedary clearly shows the high risk of introduction of infections if attention is not paid to individual and herd health status. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 SP - 511 EP - 516 T1 - Corynebacterium pseudotuberculosis infection in a dromedary (Camelus dromedarius) in Germany T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Corynebacterium pseudotuberculosis infection in a dromedary (Camelus dromedarius) in Germany TT - Corynebacterium pseudotuberculosis-Infektion bei einem Dromedar (Camelus dromedarius) in Deutschland VL - 130 SN - 0005-9366 ER -