TY - JOUR KW - Anaplasma phagocytophilum KW - tick-borne diseases KW - PCR KW - wild bison AU - B Dzięgiel AU - Ł Adaszek AU - M Krzysiak AU - M Skrzypczak AU - M Adaszek AU - B Furmaga AU - S Winiarczyk AB - The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":88693,"title":"The occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland","topline":"","teaserText":"Die Identifizierung von Anaplasma phagocytophilum bei wilden Bisons aus dem Bialowieza-Urwald in Ostpolen","content":"

Summary<\/span>
The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18\ufeff908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. <\/p>

Keywords<\/span>
Anaplasma phagocytophilum, tick-borne diseases, PCR, wild bison <\/p>

Zusammenfassung<\/span>
Ziel der Untersuchungen war es, das Vorkommen von Anaplasma spp. in einer Gruppe von 120 wilden Bisons (Bison bonasus) aus dem Bialowieza-Urwald in Ostpolen zu untersuchen und durch auf PCR basierende 16S rRNA GenSequenzierung zu bestimmen, welche Arten von Anaplasma bei diesen Tieren nachgewiesen werden k\u00f6nnen. Die PCR ergab die Anwesenheit von 16S rRNA Anaplasma spp. genetischem Material im Blut von 22 der 120 untersuchten Tiere. Die Sequenzen der PCR-Produkte zeigten eine 100%ige Homologie miteinander und 100 % Homologie mit der Anaplasma phagocytophilum GU 18\ufeff908 Sequenz aus unserer fr\u00fcheren Studie, isoliert von einem Pferd aus einem klinischen Fall von Anaplasmosis. Die Homologie der erhaltenen Sequenzen mit Sequenzen des 16S-rRNA-Gens von verschiedenen in der Genbank hinterlegten Anaplasma Arten, lag zwischen 95,8 % und 98,8 %. Basierend auf den Ergebnissen der molekularen Analyse wurde im Blut von 22 wilden Bisons bakterielle DNA von Anaplasma phagocytophilum identifiziert.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Anaplasma phagocytophilum, durch Zecken \u00fcbertragbare Krankheiten, PCR, wilde Bisons<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jul 7, 2015 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/the-occurrence-of-anaplasma-phagocytophilum-in-wild-bison-from-the-bialowieza-primeval-forest-in-eastern-poland\/150\/3130\/88693"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/the-occurrence-of-anaplasma-phagocytophilum-in-wild-bison-from-the-bialowieza-primeval-forest-in-eastern-poland\/150\/3130\/88693\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 310\u2013314 (2015) ","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-310","doiFirstPage":"310","doiLastPage":"314","doiTransmitted":true,"doiAuthor":"Dzi\u0119giel B, Adaszek \u0141, Krzysiak M, Skrzypczak M, Adaszek M, Furmaga B, Winiarczyk S","pdf":{"path":"http:\/\/data\/BMW_2015_07_08_0310.pdf","title":"BMW_2015_07_08_0310.pdf","description":"The occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland"},"authors":[{"firstName":"B","middleName":"","lastName":"Dzi\u0119giel"},{"firstName":"\u0141","middleName":"","lastName":"Adaszek"},{"firstName":"M","middleName":"","lastName":"Krzysiak"},{"firstName":"M","middleName":"","lastName":"Skrzypczak"},{"firstName":"M","middleName":"","lastName":"Adaszek"},{"firstName":"B","middleName":"","lastName":"Furmaga"},{"firstName":"S","middleName":"","lastName":"Winiarczyk"}],"contentOptimised":"

Summary<\/strong>
The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18\ufeff908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. <\/p>

Keywords:<\/strong>
Anaplasma phagocytophilum, tick-borne diseases, PCR, wild bison <\/p>

Zusammenfassung<\/strong>
Ziel der Untersuchungen war es, das Vorkommen von Anaplasma spp. in einer Gruppe von 120 wilden Bisons (Bison bonasus) aus dem Bialowieza-Urwald in Ostpolen zu untersuchen und durch auf PCR basierende 16S rRNA GenSequenzierung zu bestimmen, welche Arten von Anaplasma bei diesen Tieren nachgewiesen werden k\u00f6nnen. Die PCR ergab die Anwesenheit von 16S rRNA Anaplasma spp. genetischem Material im Blut von 22 der 120 untersuchten Tiere. Die Sequenzen der PCR-Produkte zeigten eine 100%ige Homologie miteinander und 100 % Homologie mit der Anaplasma phagocytophilum GU 18\ufeff908 Sequenz aus unserer fr\u00fcheren Studie, isoliert von einem Pferd aus einem klinischen Fall von Anaplasmosis. Die Homologie der erhaltenen Sequenzen mit Sequenzen des 16S-rRNA-Gens von verschiedenen in der Genbank hinterlegten Anaplasma Arten, lag zwischen 95,8 % und 98,8 %. Basierend auf den Ergebnissen der molekularen Analyse wurde im Blut von 22 wilden Bisons bakterielle DNA von Anaplasma phagocytophilum identifiziert.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Anaplasma phagocytophilum, durch Zecken \u00fcbertragbare Krankheiten, PCR, wilde Bisons<\/p>","primaryLanguage":"englisch","summary":"The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18\ufeff908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. <\/p>

","keywords":["Anaplasma phagocytophilum","tick-borne diseases","PCR","wild bison"],"zusammenfassung":"Ziel der Untersuchungen war es, das Vorkommen von Anaplasma spp. in einer Gruppe von 120 wilden Bisons (Bison bonasus) aus dem Bialowieza-Urwald in Ostpolen zu untersuchen und durch auf PCR basierende 16S rRNA GenSequenzierung zu bestimmen, welche Arten von Anaplasma bei diesen Tieren nachgewiesen werden k\u00f6nnen. Die PCR ergab die Anwesenheit von 16S rRNA Anaplasma spp. genetischem Material im Blut von 22 der 120 untersuchten Tiere. Die Sequenzen der PCR-Produkte zeigten eine 100%ige Homologie miteinander und 100 % Homologie mit der Anaplasma phagocytophilum GU 18\ufeff908 Sequenz aus unserer fr\u00fcheren Studie, isoliert von einem Pferd aus einem klinischen Fall von Anaplasmosis. Die Homologie der erhaltenen Sequenzen mit Sequenzen des 16S-rRNA-Gens von verschiedenen in der Genbank hinterlegten Anaplasma Arten, lag zwischen 95,8 % und 98,8 %. Basierend auf den Ergebnissen der molekularen Analyse wurde im Blut von 22 wilden Bisons bakterielle DNA von Anaplasma phagocytophilum identifiziert.<\/p>

","schluesselwoerter":["Anaplasma phagocytophilum","durch Zecken \u00fcbertragbare Krankheiten","PCR","wilde Bisons"],"translatedTitle":"Die Identifizierung von Anaplasma phagocytophilum bei wilden Bisons aus dem Bialowieza-Urwald in Ostpolen","abstractE":"The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18\ufeff908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. ","date":{"year":2015,"date":"07\/2015","accepted":"2015-07-07"},"volume":"128","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"310-314","redirects":["the-occurrence-of-anaplasma-phagocytophilum-in-wild-bison-from-the-bialowieza-primeval-forest-in-eastern-poland\/150\/3130\/88693"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 07/2015 DO - 10.2376/0005-9366-128-310 ET - 7/8 LA - English N2 - The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2015 SP - 310 EP - 314 T1 - The occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - The occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland TT - Die Identifizierung von Anaplasma phagocytophilum bei wilden Bisons aus dem Bialowieza-Urwald in Ostpolen VL - 128 SN - 0005-9366 ER -