TY - JOUR KW - infection KW - bleeding time KW - platelet function analyser KW - platelet aggregation KW - primary haemostasis KW - SIRS AU - M Abid AU - K Kalbantner AU - R Mischke AB - The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups “SIRS” and “Non-SIRS”). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen/ADP: 83 [55–301] vs. 65 [47–88] s; median [minimum–maximum]; p lt; 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 ± 26.8 vs. 67.3 ± 21.8%; mean ± SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups “SIRS” and “Non-SIRS”. In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregability may partly indicate preactivation of platelets. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":88686,"title":"Platelet function in dogs with bacterial infections and leishmaniasis","topline":"","teaserText":"Thrombozytenfunktion bei Hunden mit bakteriellen Infektionen und Leishmaniose","content":"

Summary<\/span>
The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups \u201cSIRS\u201d and \u201cNon-SIRS\u201d). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; median [minimum\u2013maximum]; p lt; 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8%; mean \u00b1 SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups \u201cSIRS\u201d and \u201cNon-SIRS\u201d. In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregability may partly indicate preactivation of platelets.<\/p>

Keywords<\/span>
infection, bleeding time, platelet function analyser, platelet aggregation, primary haemostasis, SIRS<\/p>

Zusammenfassung<\/span>
Diese Studie verfolgte das Ziel, den Einfluss von bakteriellen Infektionen sowie Leishmaniose auf die prim\u00e4re H\u00e4mostase beim Hund zu untersuchen. Kapill\u00e4re Blutungszeit, automatische Pl\u00e4ttchenfunktionsanalyse (PFA-100), turbidimetrische Pl\u00e4ttchenaggregation, Impedanzaggregometrie, Pl\u00e4ttchenzahl und zus\u00e4tzlich der H\u00e4matokrit wurden bei 25 Hunden mit bakteriellen Infektionen oder Leishmaniose untersucht. Die Ergebnisse der erkrankten Hunde wurden mit einer Kontrollgruppe verglichen und zudem erfolgte eine Unterteilung in zwei Subgruppen abh\u00e4ngig vom Vorliegen von Kriterien des \u201esystemischen respiratorischen Syndroms\u201c (Gruppen \u201cSIRS\u201d und \u201cNonSIRS\u201d). Hunde mit Infektionen hatten signifikant verl\u00e4ngerte Verschlusszeiten mit dem PFA-100 bei Verwendung beider Testzelltypen (z. B. Kollagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; Median [Minimum\u2013Maximum]; p lt; 0.0001), eine signifikante Verminderung der maximalen Aggregation der turbidimetrischen Aggregometrie (z. B. ADP-induziert: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8 %; arithmetischer Mittelwert \u00b1 Standardabweichung; p = 0.003), eine signifikant erh\u00f6hte Kollagen-induzierte Impedanzaggregometrie und eine signifikante Suppression der Arachidons\u00e4ure-induzierten Impedanzaggregometrie. Eine verst\u00e4rkte Kollagen-induzierte Impedanzaggregation war der einzige signifikante Unterschied zwischen den Subgruppen \u201eSIRS\u201d und \u201eNon-SIRS\u201d. Res\u00fcmierend zeigten die meisten In-vitro-Tests eine normale bis moderat verminderte Pl\u00e4ttchenfunktion an, einige jedoch eine gesteigerte Pl\u00e4ttchenaggregation. Die verminderte Aggregabilit\u00e4t kann teilweise eine Pr\u00e4aktivierung der Thrombozyten anzeigen.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Infektion, Blutungszeit, Pl\u00e4ttchenfunktionsanalyseger\u00e4t, Pl\u00e4ttchenaggregation, prim\u00e4re H\u00e4mostase, SIRS <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jul 7, 2015 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/platelet-function-in-dogs-with-bacterial-infections-and-leishmaniasis\/150\/3130\/88686"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/platelet-function-in-dogs-with-bacterial-infections-and-leishmaniasis\/150\/3130\/88686\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 289\u2013296 (2015) ","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-289","doiFirstPage":"289","doiLastPage":"296","doiTransmitted":true,"doiAuthor":"Abid M, Kalbantner K, Mischke R","pdf":{"path":"http:\/\/data\/BMW_2015_07_08_0289.pdf","title":"BMW_2015_07_08_0289.pdf","description":"Platelet function in dogs with bacterial infections and leishmaniasis"},"authors":[{"firstName":"M","middleName":"","lastName":"Abid"},{"firstName":"K","middleName":"","lastName":"Kalbantner"},{"firstName":"R","middleName":"","lastName":"Mischke"}],"contentOptimised":"

Summary<\/strong>
The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups \u201cSIRS\u201d and \u201cNon-SIRS\u201d). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; median [minimum\u2013maximum]; p lt; 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8%; mean \u00b1 SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups \u201cSIRS\u201d and \u201cNon-SIRS\u201d. In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregability may partly indicate preactivation of platelets.<\/p>

Keywords:<\/strong>
infection, bleeding time, platelet function analyser, platelet aggregation, primary haemostasis, SIRS<\/p>

Zusammenfassung<\/strong>
Diese Studie verfolgte das Ziel, den Einfluss von bakteriellen Infektionen sowie Leishmaniose auf die prim\u00e4re H\u00e4mostase beim Hund zu untersuchen. Kapill\u00e4re Blutungszeit, automatische Pl\u00e4ttchenfunktionsanalyse (PFA-100), turbidimetrische Pl\u00e4ttchenaggregation, Impedanzaggregometrie, Pl\u00e4ttchenzahl und zus\u00e4tzlich der H\u00e4matokrit wurden bei 25 Hunden mit bakteriellen Infektionen oder Leishmaniose untersucht. Die Ergebnisse der erkrankten Hunde wurden mit einer Kontrollgruppe verglichen und zudem erfolgte eine Unterteilung in zwei Subgruppen abh\u00e4ngig vom Vorliegen von Kriterien des \u201esystemischen respiratorischen Syndroms\u201c (Gruppen \u201cSIRS\u201d und \u201cNonSIRS\u201d). Hunde mit Infektionen hatten signifikant verl\u00e4ngerte Verschlusszeiten mit dem PFA-100 bei Verwendung beider Testzelltypen (z. B. Kollagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; Median [Minimum\u2013Maximum]; p lt; 0.0001), eine signifikante Verminderung der maximalen Aggregation der turbidimetrischen Aggregometrie (z. B. ADP-induziert: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8 %; arithmetischer Mittelwert \u00b1 Standardabweichung; p = 0.003), eine signifikant erh\u00f6hte Kollagen-induzierte Impedanzaggregometrie und eine signifikante Suppression der Arachidons\u00e4ure-induzierten Impedanzaggregometrie. Eine verst\u00e4rkte Kollagen-induzierte Impedanzaggregation war der einzige signifikante Unterschied zwischen den Subgruppen \u201eSIRS\u201d und \u201eNon-SIRS\u201d. Res\u00fcmierend zeigten die meisten In-vitro-Tests eine normale bis moderat verminderte Pl\u00e4ttchenfunktion an, einige jedoch eine gesteigerte Pl\u00e4ttchenaggregation. Die verminderte Aggregabilit\u00e4t kann teilweise eine Pr\u00e4aktivierung der Thrombozyten anzeigen.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Infektion, Blutungszeit, Pl\u00e4ttchenfunktionsanalyseger\u00e4t, Pl\u00e4ttchenaggregation, prim\u00e4re H\u00e4mostase, SIRS <\/p>","primaryLanguage":"englisch","summary":"The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups \u201cSIRS\u201d and \u201cNon-SIRS\u201d). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; median [minimum\u2013maximum]; p lt; 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8%; mean \u00b1 SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups \u201cSIRS\u201d and \u201cNon-SIRS\u201d. In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregability may partly indicate preactivation of platelets.<\/p>

","keywords":["infection","bleeding time","platelet function analyser","platelet aggregation","primary haemostasis","SIRS"],"zusammenfassung":"Diese Studie verfolgte das Ziel, den Einfluss von bakteriellen Infektionen sowie Leishmaniose auf die prim\u00e4re H\u00e4mostase beim Hund zu untersuchen. Kapill\u00e4re Blutungszeit, automatische Pl\u00e4ttchenfunktionsanalyse (PFA-100), turbidimetrische Pl\u00e4ttchenaggregation, Impedanzaggregometrie, Pl\u00e4ttchenzahl und zus\u00e4tzlich der H\u00e4matokrit wurden bei 25 Hunden mit bakteriellen Infektionen oder Leishmaniose untersucht. Die Ergebnisse der erkrankten Hunde wurden mit einer Kontrollgruppe verglichen und zudem erfolgte eine Unterteilung in zwei Subgruppen abh\u00e4ngig vom Vorliegen von Kriterien des \u201esystemischen respiratorischen Syndroms\u201c (Gruppen \u201cSIRS\u201d und \u201cNonSIRS\u201d). Hunde mit Infektionen hatten signifikant verl\u00e4ngerte Verschlusszeiten mit dem PFA-100 bei Verwendung beider Testzelltypen (z. B. Kollagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; Median [Minimum\u2013Maximum]; p lt; 0.0001), eine signifikante Verminderung der maximalen Aggregation der turbidimetrischen Aggregometrie (z. B. ADP-induziert: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8 %; arithmetischer Mittelwert \u00b1 Standardabweichung; p = 0.003), eine signifikant erh\u00f6hte Kollagen-induzierte Impedanzaggregometrie und eine signifikante Suppression der Arachidons\u00e4ure-induzierten Impedanzaggregometrie. Eine verst\u00e4rkte Kollagen-induzierte Impedanzaggregation war der einzige signifikante Unterschied zwischen den Subgruppen \u201eSIRS\u201d und \u201eNon-SIRS\u201d. Res\u00fcmierend zeigten die meisten In-vitro-Tests eine normale bis moderat verminderte Pl\u00e4ttchenfunktion an, einige jedoch eine gesteigerte Pl\u00e4ttchenaggregation. Die verminderte Aggregabilit\u00e4t kann teilweise eine Pr\u00e4aktivierung der Thrombozyten anzeigen.<\/p>

","schluesselwoerter":["Infektion","Blutungszeit","Pl\u00e4ttchenfunktionsanalyseger\u00e4t","Pl\u00e4ttchenaggregation","prim\u00e4re H\u00e4mostase","SIRS"],"translatedTitle":"Thrombozytenfunktion bei Hunden mit bakteriellen Infektionen und Leishmaniose","abstractE":"The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups \u201cSIRS\u201d and \u201cNon-SIRS\u201d). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen\/ADP: 83 [55\u2013301] vs. 65 [47\u201388] s; median [minimum\u2013maximum]; p lt; 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 \u00b1 26.8 vs. 67.3 \u00b1 21.8%; mean \u00b1 SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups \u201cSIRS\u201d and \u201cNon-SIRS\u201d. In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregability may partly indicate preactivation of platelets.","date":{"year":2015,"date":"07\/2015","accepted":"2015-07-07"},"volume":"128","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"289-296","redirects":["platelet-function-in-dogs-with-bacterial-infections-and-leishmaniasis\/150\/3130\/88686"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 07/2015 DO - 10.2376/0005-9366-128-289 ET - 7/8 LA - English N2 - The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups “SIRS” and “Non-SIRS”). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen/ADP: 83 [55–301] vs. 65 [47–88] s; median [minimum–maximum]; p lt; 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 ± 26.8 vs. 67.3 ± 21.8%; mean ± SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups “SIRS” and “Non-SIRS”. In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregability may partly indicate preactivation of platelets. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2015 SP - 289 EP - 296 T1 - Platelet function in dogs with bacterial infections and leishmaniasis T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Platelet function in dogs with bacterial infections and leishmaniasis TT - Thrombozytenfunktion bei Hunden mit bakteriellen Infektionen und Leishmaniose VL - 128 SN - 0005-9366 ER -