TY - JOUR KW - prevalence KW - breeding cat KW - real-time PCR AU - K Płoneczka-Janeczko AU - K Bierowiec AU - J Bania AU - M Kiełbowicz AU - Z Kiełbowicz AB - The prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wrocław city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wrocław (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":79890,"title":"Felid herpesvirus 1 (FHV 1) carriers among urban breeding facilities in Wroclaw (Poland)","topline":"","teaserText":"Pr\u00e4valenz von Tr\u00e4gern des Felinen Herpesvirus 1 (FHV-1) in st\u00e4dtischen Zuchten in Breslau (Polen)","content":"

Summary<\/span>
The prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wroc\u0142aw city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. <\/span>The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wroc\u0142aw (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection. <\/p>

Keywords<\/span>
prevalence, breeding cat, real-time PCR <\/p>

Zusammenfassung<\/span>
Ziel der Studie war es, die Pr\u00e4valenz von Tr\u00e4gern des Felinen Herpesvirus 1 (FHV-1) in geschlossenen, Einzelkatzen haltenden Zuchten im st\u00e4dtischen Umfeld (Breslau) zu untersuchen. Die Studie wurde in einem Zeitraum von drei Jahren (2011\u20132013) durchgef\u00fchrt. Das Vorliegen einer FHV-1-Infektion wurde mit der Real-time-Polymerasekettenreaktion mit fluoreszenzoptischer Produktdetektion (FQ-Probe) von DNA aus Augentupfern der Tiere durchgef\u00fchrt. DNA von FHV-1 wurde bei 14 (14\/67, 20,9 %) Zuchtkatzen, bei acht Tieren (8\/28) mit klinischen Symptomen einer FHV-1-Infektion und bei sechs Tieren (6\/39) ohne klinische Manifestation nachgewiesen. Die Prozentzahl positiver Tr\u00e4ger lag zwischen 12 und 44 % und war von der H\u00e4ufigkeit des Monitorings abh\u00e4ngig. Untersuchungen ergaben eine FHV-1-Pr\u00e4valenz von 20 % bei Katzen im optimalen Reproduktionsalter (2\u20137 Jahre). Einzelkatzen haltende Rassekatzentuchten in Breslau (Polen) stellen ein signifikantes Reservoir f\u00fcr FHV-1 dar. Die Zahl der Monitoringwiederholungen hat einen Einfluss auf den FHV-1-Nachweis.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Pr\u00e4valenz, Zuchtkatze, real-time PCR<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jun 27, 2014 9:13:50 PM","oldUrls":["http:\/\/vetline.de\/felid-herpesvirus-1-fhv-1-carriers-among-urban-breeding-facilities-in-wroclaw-poland\/150\/3130\/79890"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/felid-herpesvirus-1-fhv-1-carriers-among-urban-breeding-facilities-in-wroclaw-poland\/150\/3130\/79890\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 127, 243\u2013246 (2014) ","doiissn":"0005-9366 ","doiNr":"10.2376\/0005-9366-127-243","doiFirstPage":"243","doiLastPage":"246","doiTransmitted":true,"doiAuthor":"P\u0142oneczka-Janeczko K, Bierowiec K, Bania J, Kie\u0142bowicz M, Kie\u0142bowicz Z","pdf":{"path":"http:\/\/data\/BMW_2014_05_06_0243.pdf","title":"BMW_2014_05_06_0243.pdf","description":"Felid herpesvirus 1 (FHV 1) carriers among urban breeding facilities in Wroclaw (Poland) "},"authors":[{"firstName":"K","middleName":"","lastName":"P\u0142oneczka-Janeczko"},{"firstName":"K","middleName":"","lastName":"Bierowiec"},{"firstName":"J","middleName":"","lastName":"Bania"},{"firstName":"M","middleName":"","lastName":"Kie\u0142bowicz"},{"firstName":"Z","middleName":"","lastName":"Kie\u0142bowicz"}],"contentOptimised":"

Summary<\/strong>
The prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wroc\u0142aw city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. <\/em>The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wroc\u0142aw (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection. <\/p>

Keywords:<\/strong>
prevalence, breeding cat, real-time PCR <\/p>

Zusammenfassung<\/strong>
Ziel der Studie war es, die Pr\u00e4valenz von Tr\u00e4gern des Felinen Herpesvirus 1 (FHV-1) in geschlossenen, Einzelkatzen haltenden Zuchten im st\u00e4dtischen Umfeld (Breslau) zu untersuchen. Die Studie wurde in einem Zeitraum von drei Jahren (2011\u20132013) durchgef\u00fchrt. Das Vorliegen einer FHV-1-Infektion wurde mit der Real-time-Polymerasekettenreaktion mit fluoreszenzoptischer Produktdetektion (FQ-Probe) von DNA aus Augentupfern der Tiere durchgef\u00fchrt. DNA von FHV-1 wurde bei 14 (14\/67, 20,9 %) Zuchtkatzen, bei acht Tieren (8\/28) mit klinischen Symptomen einer FHV-1-Infektion und bei sechs Tieren (6\/39) ohne klinische Manifestation nachgewiesen. Die Prozentzahl positiver Tr\u00e4ger lag zwischen 12 und 44 % und war von der H\u00e4ufigkeit des Monitorings abh\u00e4ngig. Untersuchungen ergaben eine FHV-1-Pr\u00e4valenz von 20 % bei Katzen im optimalen Reproduktionsalter (2\u20137 Jahre). Einzelkatzen haltende Rassekatzentuchten in Breslau (Polen) stellen ein signifikantes Reservoir f\u00fcr FHV-1 dar. Die Zahl der Monitoringwiederholungen hat einen Einfluss auf den FHV-1-Nachweis.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Pr\u00e4valenz, Zuchtkatze, real-time PCR<\/p>","primaryLanguage":"englisch","summary":"The prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wroc\u0142aw city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. <\/em>The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wroc\u0142aw (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection. <\/p>

","keywords":["prevalence","breeding cat","real-time PCR"],"zusammenfassung":"Ziel der Studie war es, die Pr\u00e4valenz von Tr\u00e4gern des Felinen Herpesvirus 1 (FHV-1) in geschlossenen, Einzelkatzen haltenden Zuchten im st\u00e4dtischen Umfeld (Breslau) zu untersuchen. Die Studie wurde in einem Zeitraum von drei Jahren (2011\u20132013) durchgef\u00fchrt. Das Vorliegen einer FHV-1-Infektion wurde mit der Real-time-Polymerasekettenreaktion mit fluoreszenzoptischer Produktdetektion (FQ-Probe) von DNA aus Augentupfern der Tiere durchgef\u00fchrt. DNA von FHV-1 wurde bei 14 (14\/67, 20,9 %) Zuchtkatzen, bei acht Tieren (8\/28) mit klinischen Symptomen einer FHV-1-Infektion und bei sechs Tieren (6\/39) ohne klinische Manifestation nachgewiesen. Die Prozentzahl positiver Tr\u00e4ger lag zwischen 12 und 44 % und war von der H\u00e4ufigkeit des Monitorings abh\u00e4ngig. Untersuchungen ergaben eine FHV-1-Pr\u00e4valenz von 20 % bei Katzen im optimalen Reproduktionsalter (2\u20137 Jahre). Einzelkatzen haltende Rassekatzentuchten in Breslau (Polen) stellen ein signifikantes Reservoir f\u00fcr FHV-1 dar. Die Zahl der Monitoringwiederholungen hat einen Einfluss auf den FHV-1-Nachweis.<\/p>

","schluesselwoerter":["Pr\u00e4valenz","Zuchtkatze","real-time PCR"],"translatedTitle":"Pr\u00e4valenz von Tr\u00e4gern des Felinen Herpesvirus 1 (FHV-1) in st\u00e4dtischen Zuchten in Breslau (Polen)","abstractE":"The prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wroc\u0142aw city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wroc\u0142aw (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection. ","date":{"year":2014,"date":"06\/2014","accepted":"2014-06-27"},"volume":"127","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"243-246","redirects":["felid-herpesvirus-1-fhv-1-carriers-among-urban-breeding-facilities-in-wroclaw-poland\/150\/3130\/79890"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 06/2014 DO - 10.2376/0005-9366-127-243 LA - English N2 - The prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wrocław city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wrocław (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2014 SP - 243 EP - 246 T1 - Felid herpesvirus 1 (FHV 1) carriers among urban breeding facilities in Wroclaw (Poland) T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Felid herpesvirus 1 (FHV 1) carriers among urban breeding facilities in Wroclaw (Poland) TT - Prävalenz von Trägern des Felinen Herpesvirus 1 (FHV-1) in städtischen Zuchten in Breslau (Polen) VL - 127 SN - 0005-9366 ER -