TY - JOUR KW - Q fever KW - ELISA KW - PCR KW - seroconversion KW - seronegativization KW - colostral antibodies AU - M Freick AU - A Konrath AU - H Enbergs AU - R Diller AU - J Weber AU - J Walraph AB - Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, is widely distributed in cattle herds. In this study, we investigated changes in antibody response during dry period in dairy cows and the course of maternal antibody levels in dairy calves by using a commercial ELISA. In a total of 35 pluriparous cows, the proportion of seropositive animals (65.7% vs. 42.9%) (p = 0.039) and the mean antibody score levels (2.8 ± 2.4 vs. 1.0 ± 1.4) (p lt; 0.001) declined significantly from dry cow therapy to calving. During this period, 2/12 (16.7%) formerly seronegative cows seroconverted, whereas in 10/23 (43.5%) of the seropositive animals a seronegativization occurred. C. burnetii DNA was detected by PCR in 15/35 (42.8%) of the vaginal swabs obtained immediately after calving. All twelve calves enclosed in this study were born seronegative and precolostral whole blood samples of the calves were tested negative by PCR for C. burnetii DNA. After colostrum intake, 7/12 (58.3%) of these calves seroconverted and stayed seropositive until day 33. Afterwards, the proportion of seropositive calves and the antibody scores decreased gradually. From day 103 after birth on, all calves remained seronegative. In conclusion, we recommend the exclusion of cows for the period of colostrogenesis and calves after colostrum intake from seroepidemiological investigations to avoid bias. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":104353,"title":"Coxiella burnetii: Decline of antibody response in pluriparous dairy cows during dry period and maternal antibody levels in dairy calves in an endemically infected herd","topline":"","teaserText":"Coxiella burnetii: Absinken der Antik\u00f6rperreaktion bei pluriparen Milchk\u00fchen w\u00e4hrend der Trockenstehphase und maternale Antik\u00f6rperspiegel bei Milchrindk\u00e4lbern in einer endemisch infizierten Herde","content":"

Summary<\/span>
Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, is widely distributed in cattle herds. In this study, we investigated changes in antibody response during dry period in dairy cows and the course of maternal antibody levels in dairy calves by using a commercial ELISA. In a total of 35 pluriparous cows, the proportion of seropositive animals (65.7% vs. 42.9%) (p = 0.039) and the mean antibody score levels (2.8 \u00b1 2.4 vs. 1.0 \u00b1 1.4) (p lt; 0.001) declined significantly from dry cow therapy to calving. During this period, 2\/12 (16.7%) formerly seronegative cows seroconverted, whereas in 10\/23 (43.5%) of the seropositive animals a seronegativization occurred. C. burnetii DNA was detected by PCR in 15\/35 (42.8%) of the vaginal swabs obtained immediately after calving. All twelve calves enclosed in this study were born seronegative and precolostral whole blood samples of the calves were tested negative by PCR for C. burnetii DNA. After colostrum intake, 7\/12 (58.3%) of these calves seroconverted and stayed seropositive until day 33. Afterwards, the proportion of seropositive calves and the antibody scores decreased gradually. From day 103 after birth on, all calves remained seronegative. In conclusion, we recommend the exclusion of cows for the period of colostrogenesis and calves after colostrum intake from seroepidemiological investigations to avoid bias.<\/p>

Keywords<\/span>
Q fever, ELISA, PCR, seroconversion, seronegativization, colostral antibodies<\/p>

Zusammenfassung<\/span>
Coxiella burnetii (C. burnetii), ein intrazellul\u00e4res zoonotisches Bakterium und Erreger des Q-Fiebers, ist in Rinderherden weit verbreitet. In dieser Studie wurden \u00c4nderungen der Antik\u00f6rperreaktion bei Milchk\u00fchen w\u00e4hrend der Trockenstehphase und der Verlauf maternaler Antik\u00f6rperspiegel bei Milchrindk\u00e4lbern mittels eines kommerziell erh\u00e4ltlichen ELISAs untersucht. Innerhalb einer Gruppe von 35 pluriparen K\u00fchen sanken sowohl der Anteil seropositiver Tiere (65,7 % vs. 42,9 %) (p = 0,039) als auch der mittlere Antik\u00f6rperscore (2,8 \u00b1 2,4 vs. 1,0 \u00b1 1,4) (p lt; 0.001) im Zeitraum vom Trockenstellen bis zur Abkalbung signifikant. In dieser Periode serokonvertierten 2\/12 (16.7%) der urspr\u00fcnglich seronegativen K\u00fche, w\u00e4hrend 10\/23 (43.5 %) der seropositiven Tiere seronegativ wurden. Die DNA von C. burnetii konnte durch eine PCR in 15\/35 (42.8%) der unmittelbar nach der Kalbung gewonnenen Vaginaltupferproben nachgewiesen werden. Alle zw\u00f6lf in die Studie eingeschlossenen K\u00e4lber wurden seronegativ geboren. Auch das Genom von C. burnetii konnte in pr\u00e4kolostralen Vollblutproben mittels PCR nicht detektiert werden. Nach der Kolostrumaufnahme serokonvertierten 7\/12 (58.3 %) der K\u00e4lber und blieben bis zum 33. Lebenstag seropositiv. Anschlie\u00dfend fielen der Anteil seropositiver Tiere und die Antik\u00f6rperscores allm\u00e4hlich ab. Ab dem 103. Lebenstag waren alle K\u00e4lber wieder seronegativ. Schlussfolgernd wird empfohlen, K\u00fche w\u00e4hrend der Phase der Kolostrogenese und K\u00e4lber nach der Kolostrumaufnahme von seroepidemiologischen Studien auszuschlie\u00dfen, um Verzerrungen zu vermeiden. <\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Q-Fieber, ELISA, PCR, Serokonversion, Seronegativierung, kolostrale Antik\u00f6rper<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Sep 14, 2017 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/coxiella-burnetii-decline-of-antibody-response-in-pluriparous-dairy-cows-during-dry-period-and-maternal-antibody-levels-in-dairy-calves-in-an-endemically-infected-herd\/150\/3130\/104353"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift ","doiDocumentUri":"http:\/\/www.vetline.de\/coxiella-burnetii-decline-of-antibody-response-in-pluriparous-dairy-cows-during-dry-period-and-maternal-antibody-levels-in-dairy-calves-in-an-endemically-infected-herd\/150\/3130\/104353\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 130, Heft 9\/10 (2017), Seiten 388\u2013394","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16037","doiFirstPage":"388","doiLastPage":"394","doiTransmitted":true,"doiAuthor":"Freick M, Konrath A, Enbergs H, Diller R, Weber J, Walraph J","pdf":{"path":"http:\/\/data\/BMW_2017_09_10_0388.pdf","title":"BMW_2017_09_10_0388.pdf","description":"Coxiella burnetii: Decline of antibody response in pluriparous dairy cows during dry period and maternal antibody levels in dairy calves in an endemically infected herd"},"authors":[{"firstName":"M","middleName":"","lastName":"Freick"},{"firstName":"A","middleName":"","lastName":"Konrath"},{"firstName":"H","middleName":"","lastName":"Enbergs"},{"firstName":"R","middleName":"","lastName":"Diller"},{"firstName":"J","middleName":"","lastName":"Weber"},{"firstName":"J","middleName":"","lastName":"Walraph"}],"contentOptimised":"

Summary<\/strong>
Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, is widely distributed in cattle herds. In this study, we investigated changes in antibody response during dry period in dairy cows and the course of maternal antibody levels in dairy calves by using a commercial ELISA. In a total of 35 pluriparous cows, the proportion of seropositive animals (65.7% vs. 42.9%) (p = 0.039) and the mean antibody score levels (2.8 \u00b1 2.4 vs. 1.0 \u00b1 1.4) (p lt; 0.001) declined significantly from dry cow therapy to calving. During this period, 2\/12 (16.7%) formerly seronegative cows seroconverted, whereas in 10\/23 (43.5%) of the seropositive animals a seronegativization occurred. C. burnetii DNA was detected by PCR in 15\/35 (42.8%) of the vaginal swabs obtained immediately after calving. All twelve calves enclosed in this study were born seronegative and precolostral whole blood samples of the calves were tested negative by PCR for C. burnetii DNA. After colostrum intake, 7\/12 (58.3%) of these calves seroconverted and stayed seropositive until day 33. Afterwards, the proportion of seropositive calves and the antibody scores decreased gradually. From day 103 after birth on, all calves remained seronegative. In conclusion, we recommend the exclusion of cows for the period of colostrogenesis and calves after colostrum intake from seroepidemiological investigations to avoid bias.<\/p>

Keywords:<\/strong>
Q fever, ELISA, PCR, seroconversion, seronegativization, colostral antibodies<\/p>

Zusammenfassung<\/strong>
Coxiella burnetii (C. burnetii), ein intrazellul\u00e4res zoonotisches Bakterium und Erreger des Q-Fiebers, ist in Rinderherden weit verbreitet. In dieser Studie wurden \u00c4nderungen der Antik\u00f6rperreaktion bei Milchk\u00fchen w\u00e4hrend der Trockenstehphase und der Verlauf maternaler Antik\u00f6rperspiegel bei Milchrindk\u00e4lbern mittels eines kommerziell erh\u00e4ltlichen ELISAs untersucht. Innerhalb einer Gruppe von 35 pluriparen K\u00fchen sanken sowohl der Anteil seropositiver Tiere (65,7 % vs. 42,9 %) (p = 0,039) als auch der mittlere Antik\u00f6rperscore (2,8 \u00b1 2,4 vs. 1,0 \u00b1 1,4) (p lt; 0.001) im Zeitraum vom Trockenstellen bis zur Abkalbung signifikant. In dieser Periode serokonvertierten 2\/12 (16.7%) der urspr\u00fcnglich seronegativen K\u00fche, w\u00e4hrend 10\/23 (43.5 %) der seropositiven Tiere seronegativ wurden. Die DNA von C. burnetii konnte durch eine PCR in 15\/35 (42.8%) der unmittelbar nach der Kalbung gewonnenen Vaginaltupferproben nachgewiesen werden. Alle zw\u00f6lf in die Studie eingeschlossenen K\u00e4lber wurden seronegativ geboren. Auch das Genom von C. burnetii konnte in pr\u00e4kolostralen Vollblutproben mittels PCR nicht detektiert werden. Nach der Kolostrumaufnahme serokonvertierten 7\/12 (58.3 %) der K\u00e4lber und blieben bis zum 33. Lebenstag seropositiv. Anschlie\u00dfend fielen der Anteil seropositiver Tiere und die Antik\u00f6rperscores allm\u00e4hlich ab. Ab dem 103. Lebenstag waren alle K\u00e4lber wieder seronegativ. Schlussfolgernd wird empfohlen, K\u00fche w\u00e4hrend der Phase der Kolostrogenese und K\u00e4lber nach der Kolostrumaufnahme von seroepidemiologischen Studien auszuschlie\u00dfen, um Verzerrungen zu vermeiden. <\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Q-Fieber, ELISA, PCR, Serokonversion, Seronegativierung, kolostrale Antik\u00f6rper<\/p>","primaryLanguage":"englisch","summary":"Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, is widely distributed in cattle herds. In this study, we investigated changes in antibody response during dry period in dairy cows and the course of maternal antibody levels in dairy calves by using a commercial ELISA. In a total of 35 pluriparous cows, the proportion of seropositive animals (65.7% vs. 42.9%) (p = 0.039) and the mean antibody score levels (2.8 \u00b1 2.4 vs. 1.0 \u00b1 1.4) (p lt; 0.001) declined significantly from dry cow therapy to calving. During this period, 2\/12 (16.7%) formerly seronegative cows seroconverted, whereas in 10\/23 (43.5%) of the seropositive animals a seronegativization occurred. C. burnetii DNA was detected by PCR in 15\/35 (42.8%) of the vaginal swabs obtained immediately after calving. All twelve calves enclosed in this study were born seronegative and precolostral whole blood samples of the calves were tested negative by PCR for C. burnetii DNA. After colostrum intake, 7\/12 (58.3%) of these calves seroconverted and stayed seropositive until day 33. Afterwards, the proportion of seropositive calves and the antibody scores decreased gradually. From day 103 after birth on, all calves remained seronegative. In conclusion, we recommend the exclusion of cows for the period of colostrogenesis and calves after colostrum intake from seroepidemiological investigations to avoid bias.<\/p>

","keywords":["Q fever","ELISA","PCR","seroconversion","seronegativization","colostral antibodies"],"zusammenfassung":"Coxiella burnetii (C. burnetii), ein intrazellul\u00e4res zoonotisches Bakterium und Erreger des Q-Fiebers, ist in Rinderherden weit verbreitet. In dieser Studie wurden \u00c4nderungen der Antik\u00f6rperreaktion bei Milchk\u00fchen w\u00e4hrend der Trockenstehphase und der Verlauf maternaler Antik\u00f6rperspiegel bei Milchrindk\u00e4lbern mittels eines kommerziell erh\u00e4ltlichen ELISAs untersucht. Innerhalb einer Gruppe von 35 pluriparen K\u00fchen sanken sowohl der Anteil seropositiver Tiere (65,7 % vs. 42,9 %) (p = 0,039) als auch der mittlere Antik\u00f6rperscore (2,8 \u00b1 2,4 vs. 1,0 \u00b1 1,4) (p lt; 0.001) im Zeitraum vom Trockenstellen bis zur Abkalbung signifikant. In dieser Periode serokonvertierten 2\/12 (16.7%) der urspr\u00fcnglich seronegativen K\u00fche, w\u00e4hrend 10\/23 (43.5 %) der seropositiven Tiere seronegativ wurden. Die DNA von C. burnetii konnte durch eine PCR in 15\/35 (42.8%) der unmittelbar nach der Kalbung gewonnenen Vaginaltupferproben nachgewiesen werden. Alle zw\u00f6lf in die Studie eingeschlossenen K\u00e4lber wurden seronegativ geboren. Auch das Genom von C. burnetii konnte in pr\u00e4kolostralen Vollblutproben mittels PCR nicht detektiert werden. Nach der Kolostrumaufnahme serokonvertierten 7\/12 (58.3 %) der K\u00e4lber und blieben bis zum 33. Lebenstag seropositiv. Anschlie\u00dfend fielen der Anteil seropositiver Tiere und die Antik\u00f6rperscores allm\u00e4hlich ab. Ab dem 103. Lebenstag waren alle K\u00e4lber wieder seronegativ. Schlussfolgernd wird empfohlen, K\u00fche w\u00e4hrend der Phase der Kolostrogenese und K\u00e4lber nach der Kolostrumaufnahme von seroepidemiologischen Studien auszuschlie\u00dfen, um Verzerrungen zu vermeiden. <\/p>

","schluesselwoerter":["Q-Fieber","ELISA","PCR","Serokonversion","Seronegativierung","kolostrale Antik\u00f6rper"],"translatedTitle":"Coxiella burnetii: Absinken der Antik\u00f6rperreaktion bei pluriparen Milchk\u00fchen w\u00e4hrend der Trockenstehphase und maternale Antik\u00f6rperspiegel bei Milchrindk\u00e4lbern in einer endemisch infizierten Herde","abstractE":"Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, is widely distributed in cattle herds. In this study, we investigated changes in antibody response during dry period in dairy cows and the course of maternal antibody levels in dairy calves by using a commercial ELISA. In a total of 35 pluriparous cows, the proportion of seropositive animals (65.7% vs. 42.9%) (p = 0.039) and the mean antibody score levels (2.8 \u00b1 2.4 vs. 1.0 \u00b1 1.4) (p lt; 0.001) declined significantly from dry cow therapy to calving. During this period, 2\/12 (16.7%) formerly seronegative cows seroconverted, whereas in 10\/23 (43.5%) of the seropositive animals a seronegativization occurred. C. burnetii DNA was detected by PCR in 15\/35 (42.8%) of the vaginal swabs obtained immediately after calving. All twelve calves enclosed in this study were born seronegative and precolostral whole blood samples of the calves were tested negative by PCR for C. burnetii DNA. After colostrum intake, 7\/12 (58.3%) of these calves seroconverted and stayed seropositive until day 33. Afterwards, the proportion of seropositive calves and the antibody scores decreased gradually. From day 103 after birth on, all calves remained seronegative. In conclusion, we recommend the exclusion of cows for the period of colostrogenesis and calves after colostrum intake from seroepidemiological investigations to avoid bias.","date":{"year":2017,"date":"09\/2017","accepted":"2017-09-14"},"volume":"130","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"388-394","redirects":["coxiella-burnetii-decline-of-antibody-response-in-pluriparous-dairy-cows-during-dry-period-and-maternal-antibody-levels-in-dairy-calves-in-an-endemically-infected-herd\/150\/3130\/104353"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 09/2017 DO - 10.2376/0005-9366-16037 ET - 9/10 LA - English N2 - Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, is widely distributed in cattle herds. In this study, we investigated changes in antibody response during dry period in dairy cows and the course of maternal antibody levels in dairy calves by using a commercial ELISA. In a total of 35 pluriparous cows, the proportion of seropositive animals (65.7% vs. 42.9%) (p = 0.039) and the mean antibody score levels (2.8 ± 2.4 vs. 1.0 ± 1.4) (p lt; 0.001) declined significantly from dry cow therapy to calving. During this period, 2/12 (16.7%) formerly seronegative cows seroconverted, whereas in 10/23 (43.5%) of the seropositive animals a seronegativization occurred. C. burnetii DNA was detected by PCR in 15/35 (42.8%) of the vaginal swabs obtained immediately after calving. All twelve calves enclosed in this study were born seronegative and precolostral whole blood samples of the calves were tested negative by PCR for C. burnetii DNA. After colostrum intake, 7/12 (58.3%) of these calves seroconverted and stayed seropositive until day 33. Afterwards, the proportion of seropositive calves and the antibody scores decreased gradually. From day 103 after birth on, all calves remained seronegative. In conclusion, we recommend the exclusion of cows for the period of colostrogenesis and calves after colostrum intake from seroepidemiological investigations to avoid bias. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 SP - 388 EP - 394 T1 - Coxiella burnetii: Decline of antibody response in pluriparous dairy cows during dry period and maternal antibody levels in dairy calves in an endemically infected herd T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Coxiella burnetii: Decline of antibody response in pluriparous dairy cows during dry period and maternal antibody levels in dairy calves in an endemically infected herd TT - Coxiella burnetii: Absinken der Antikörperreaktion bei pluriparen Milchkühen während der Trockenstehphase und maternale Antikörperspiegel bei Milchrindkälbern in einer endemisch infizierten Herde VL - 130 SN - 0005-9366 ER -