TY - JOUR KW - MALDI-TOF MS KW - FT-IR KW - rpoB KW - 16S rRNA KW - diphtheria toxin AU - R Sting AU - S Ketterer-Pintur AU - M Contzen AU - N Mauder AU - A Sing AU - C Süß-Dombrowski AB - Corynebacterium (C.) ulcerans could be isolated from the spleen of a red fox (Vulpes vulpes) that had been found dead in the state of Baden-Württemberg, Germany. Pathohistological examination suggested that the fox had died of distemper, as was confirmed by PCR. The isolate was identified biochemically, by MALDI-TOF MS, FT-IR and by partial 16S rRNA, rpoB and tox gene sequencing. Using the Elek test the C. ulcerans isolate demonstrated diphtheria toxin production. FT-IR and sequencing data obtained from the C. ulcerans isolate from the red fox showed higher similarity to isolates from humans than to those from wild game. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":87702,"title":"Toxigenic Corynebacterium ulcerans isolated from a free-roaming red fox (Vulpes vulpes)","topline":"","teaserText":"Toxigenes Corynebacterium ulcerans-Isolat von einem freilebendem Rotfuchs (Vulpes vulpes)","content":"

Summary<\/span>
Corynebacterium (C.) ulcerans could be isolated from the spleen of a red fox (Vulpes vulpes) that had been found dead in the state of Baden-W\u00fcrttemberg, Germany. Pathohistological examination suggested that the fox had died of distemper, as was confirmed by PCR. The isolate was identified biochemically, by MALDI-TOF MS, FT-IR and by partial 16S rRNA, rpoB and tox gene sequencing. Using the Elek test the C. ulcerans isolate demonstrated diphtheria toxin production. FT-IR and sequencing data obtained from the C. ulcerans isolate from the red fox showed higher similarity to isolates from humans than to those from wild game.<\/p>

Keywords<\/span>
MALDI-TOF MS, FT-IR, rpoB, 16S rRNA, diphtheria toxin<\/p>

Zusammenfassung<\/span>
Corynebacterium (C.) ulcerans konnte aus der Milz eines Rotfuchses (Vulpes vulpes) isoliert werden, der tot in Baden-W\u00fcrttemberg, Deutschland, gefunden worden war. Pathohistologische Untersuchungen ergaben, dass der Fuchs an Staupe verendet war, was mittels PCR-Untersuchungen best\u00e4tigt werden konnte. Das Isolat wurde biochemisch, mittels MALDI-TOF MS- und FT-IR-Analysen sowie durch partielle Sequenzierungen der 16S rRNA-, rpoB- and tox-Gene identifiziert. Im ElekTest konnte die Bildung von Diphtherietoxin durch das C. ulcerans-Isolat nachgewiesen werden. Die Auswertungen der Ergebnisse der FT-IR-Untersuchungen und der DNA-Sequenzierungen ergaben eine gr\u00f6\u00dfere \u00c4hnlichkeit des C. ulcerans-Isolates mit Human- als mit Wildtierisolaten.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
MALDI-TOF MS, FT-IR, rpoB, 16S rRNA, Diphtherietoxin<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"May 21, 2015 9:55:22 AM","oldUrls":["http:\/\/vetline.de\/toxigenic-corynebacterium-ulcerans-isolated-from-a-free-roaming-red-fox-vulpes-vulpes\/150\/3130\/87702"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/toxigenic-corynebacterium-ulcerans-isolated-from-a-free-roaming-red-fox-vulpes-vulpes\/150\/3130\/87702\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 204\u2013208 (2015) ","doiissn":"0005-9366 ","doiNr":"10.2376\/0005-9366-128-204","doiFirstPage":"204","doiLastPage":"208","doiTransmitted":true,"doiAuthor":"Sting R, Ketterer-Pintur S, Contzen M, Mauder N, Sing A, S\u00fc\u00df-Dombrowski C","pdf":{"path":"http:\/\/data\/BMW_2015_05_06_0204.pdf","title":"BMW_2015_05_06_0204.pdf","description":"Toxigenic Corynebacterium ulcerans isolated from a free-roaming red fox (Vulpes vulpes)"},"authors":[{"firstName":"R","middleName":"","lastName":"Sting"},{"firstName":"S","middleName":"","lastName":"Ketterer-Pintur"},{"firstName":"M","middleName":"","lastName":"Contzen"},{"firstName":"N","middleName":"","lastName":"Mauder"},{"firstName":"A","middleName":"","lastName":"Sing"},{"firstName":"C","middleName":"","lastName":"S\u00fc\u00df-Dombrowski"}],"contentOptimised":"

Summary<\/strong>
Corynebacterium (C.) ulcerans could be isolated from the spleen of a red fox (Vulpes vulpes) that had been found dead in the state of Baden-W\u00fcrttemberg, Germany. Pathohistological examination suggested that the fox had died of distemper, as was confirmed by PCR. The isolate was identified biochemically, by MALDI-TOF MS, FT-IR and by partial 16S rRNA, rpoB and tox gene sequencing. Using the Elek test the C. ulcerans isolate demonstrated diphtheria toxin production. FT-IR and sequencing data obtained from the C. ulcerans isolate from the red fox showed higher similarity to isolates from humans than to those from wild game.<\/p>

Keywords:<\/strong>
MALDI-TOF MS, FT-IR, rpoB, 16S rRNA, diphtheria toxin<\/p>

Zusammenfassung<\/strong>
Corynebacterium (C.) ulcerans konnte aus der Milz eines Rotfuchses (Vulpes vulpes) isoliert werden, der tot in Baden-W\u00fcrttemberg, Deutschland, gefunden worden war. Pathohistologische Untersuchungen ergaben, dass der Fuchs an Staupe verendet war, was mittels PCR-Untersuchungen best\u00e4tigt werden konnte. Das Isolat wurde biochemisch, mittels MALDI-TOF MS- und FT-IR-Analysen sowie durch partielle Sequenzierungen der 16S rRNA-, rpoB- and tox-Gene identifiziert. Im ElekTest konnte die Bildung von Diphtherietoxin durch das C. ulcerans-Isolat nachgewiesen werden. Die Auswertungen der Ergebnisse der FT-IR-Untersuchungen und der DNA-Sequenzierungen ergaben eine gr\u00f6\u00dfere \u00c4hnlichkeit des C. ulcerans-Isolates mit Human- als mit Wildtierisolaten.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
MALDI-TOF MS, FT-IR, rpoB, 16S rRNA, Diphtherietoxin<\/p>","primaryLanguage":"englisch","summary":"Corynebacterium (C.) ulcerans could be isolated from the spleen of a red fox (Vulpes vulpes) that had been found dead in the state of Baden-W\u00fcrttemberg, Germany. Pathohistological examination suggested that the fox had died of distemper, as was confirmed by PCR. The isolate was identified biochemically, by MALDI-TOF MS, FT-IR and by partial 16S rRNA, rpoB and tox gene sequencing. Using the Elek test the C. ulcerans isolate demonstrated diphtheria toxin production. FT-IR and sequencing data obtained from the C. ulcerans isolate from the red fox showed higher similarity to isolates from humans than to those from wild game.<\/p>

","keywords":["MALDI-TOF MS","FT-IR","rpoB","16S rRNA","diphtheria toxin"],"zusammenfassung":"Corynebacterium (C.) ulcerans konnte aus der Milz eines Rotfuchses (Vulpes vulpes) isoliert werden, der tot in Baden-W\u00fcrttemberg, Deutschland, gefunden worden war. Pathohistologische Untersuchungen ergaben, dass der Fuchs an Staupe verendet war, was mittels PCR-Untersuchungen best\u00e4tigt werden konnte. Das Isolat wurde biochemisch, mittels MALDI-TOF MS- und FT-IR-Analysen sowie durch partielle Sequenzierungen der 16S rRNA-, rpoB- and tox-Gene identifiziert. Im ElekTest konnte die Bildung von Diphtherietoxin durch das C. ulcerans-Isolat nachgewiesen werden. Die Auswertungen der Ergebnisse der FT-IR-Untersuchungen und der DNA-Sequenzierungen ergaben eine gr\u00f6\u00dfere \u00c4hnlichkeit des C. ulcerans-Isolates mit Human- als mit Wildtierisolaten.<\/p>

","schluesselwoerter":["MALDI-TOF MS","FT-IR","rpoB","16S rRNA","Diphtherietoxin"],"translatedTitle":"Toxigenes Corynebacterium ulcerans-Isolat von einem freilebendem Rotfuchs (Vulpes vulpes)","abstractE":"Corynebacterium (C.) ulcerans could be isolated from the spleen of a red fox (Vulpes vulpes) that had been found dead in the state of Baden-W\u00fcrttemberg, Germany. Pathohistological examination suggested that the fox had died of distemper, as was confirmed by PCR. The isolate was identified biochemically, by MALDI-TOF MS, FT-IR and by partial 16S rRNA, rpoB and tox gene sequencing. Using the Elek test the C. ulcerans isolate demonstrated diphtheria toxin production. FT-IR and sequencing data obtained from the C. ulcerans isolate from the red fox showed higher similarity to isolates from humans than to those from wild game.","date":{"year":2015,"date":"05\/2015","accepted":"2015-05-21"},"volume":"128","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"204-208","redirects":["toxigenic-corynebacterium-ulcerans-isolated-from-a-free-roaming-red-fox-vulpes-vulpes\/150\/3130\/87702"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 05/2015 DO - 10.2376/0005-9366-128-204 ET - 5/6 LA - English N2 - Corynebacterium (C.) ulcerans could be isolated from the spleen of a red fox (Vulpes vulpes) that had been found dead in the state of Baden-Württemberg, Germany. Pathohistological examination suggested that the fox had died of distemper, as was confirmed by PCR. The isolate was identified biochemically, by MALDI-TOF MS, FT-IR and by partial 16S rRNA, rpoB and tox gene sequencing. Using the Elek test the C. ulcerans isolate demonstrated diphtheria toxin production. FT-IR and sequencing data obtained from the C. ulcerans isolate from the red fox showed higher similarity to isolates from humans than to those from wild game. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2015 SP - 204 EP - 208 T1 - Toxigenic Corynebacterium ulcerans isolated from a free-roaming red fox (Vulpes vulpes) T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Toxigenic Corynebacterium ulcerans isolated from a free-roaming red fox (Vulpes vulpes) TT - Toxigenes Corynebacterium ulcerans-Isolat von einem freilebendem Rotfuchs (Vulpes vulpes) VL - 128 SN - 0005-9366 ER -