TY - JOUR AU - B Makoschey AU - M Beer AB - It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":71153,"title":"A live Bovine Herpesvirus-1 marker vaccine is not shed after intramuscular vaccination","teaserText":"DOI 10.2376\/0005-9366-120-480","content":"

Summary<\/span>
It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and\/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted.


Zusammenfassung<\/span>
Mit Hilfe der hier vorgestellten Untersuchung sollte festgestellt werden, ob Rinder nach intramuskul\u00e4rer (IM) Impfung mit einer attenuierten Bovinen Herpesvirus Typ 1 (BHV-1)-Vakzine eine Vir\u00e4mie zeigen und\/oder Impfvirus mit dem Nasensekret ausscheiden. F\u00fcnf BHV-1-seronegative Rinder wurden hierf\u00fcr IM mit einer \u00dcberdosierung des Impfstoffes (BovilisR IBR marker live) immunisiert. Nasentupfer und Blutproben wurden in festgelegten Intervallen entnommen und im BHV-1-Virusnachweis getestet. Zus\u00e4tzlich wurde eine sensitive, f\u00fcr BHV-1-DNA spezifische, Polymerasekettenreaktion (PCR) mit Blutproben der inokulierten Tiere durchgef\u00fchrt. Die zun\u00e4chst BHV-1-negativen Tiere reagierten alle mit neutralisierenden Antik\u00f6rpern auf die Impfung. Dennoch konnte weder aus den Nasentupfern noch aus den Blutproben BHV-1-Impfvirus isoliert werden. Zudem konnte in den Blutproben keine BHV-1-spezifische DNA nachgewiesen werden. Aus diesen Ergebnissen l\u00e4sst sich schlussfolgern, dass das verwendete BHV-1-Impfvirus nach intramuskul\u00e4rer Applikation nicht mit dem Nasensekret ausgeschieden wurde und es nicht zur nachweisbaren Vir\u00e4mie in den Impflingen gekommen ist. Um die Ausscheidung von attenuiertem BHV-1-Impfvirus zu vermeiden, sollte die genannte attenuierte BHV-1 Markervakzine intramuskul\u00e4r verabreicht werden.<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Nov 1, 2007 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/21593703\/150\/3130\/71153"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/21593703\/150\/3130\/71153","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 120: 11-12, 480-482 (2007)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-120-480","doiFirstPage":"480","doiLastPage":"482","doiTransmitted":true,"doiAuthor":"Makoschey B, Beer M","pdf":{"path":"http:\/\/data\/bmtw_2007_11_0480.pdf","title":"bmtw_2007_11_0480.pdf","description":"A live Bovine Herpesvirus-1 marker vaccine is not shed after intramuscular vaccination

"},"authors":[{"firstName":"B","middleName":"","lastName":"Makoschey"},{"firstName":"M","middleName":"","lastName":"Beer"}],"contentOptimised":"

Summary<\/strong>
It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and\/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted.


Zusammenfassung<\/strong>
Mit Hilfe der hier vorgestellten Untersuchung sollte festgestellt werden, ob Rinder nach intramuskul\u00e4rer (IM) Impfung mit einer attenuierten Bovinen Herpesvirus Typ 1 (BHV-1)-Vakzine eine Vir\u00e4mie zeigen und\/oder Impfvirus mit dem Nasensekret ausscheiden. F\u00fcnf BHV-1-seronegative Rinder wurden hierf\u00fcr IM mit einer \u00dcberdosierung des Impfstoffes (BovilisR IBR marker live) immunisiert. Nasentupfer und Blutproben wurden in festgelegten Intervallen entnommen und im BHV-1-Virusnachweis getestet. Zus\u00e4tzlich wurde eine sensitive, f\u00fcr BHV-1-DNA spezifische, Polymerasekettenreaktion (PCR) mit Blutproben der inokulierten Tiere durchgef\u00fchrt. Die zun\u00e4chst BHV-1-negativen Tiere reagierten alle mit neutralisierenden Antik\u00f6rpern auf die Impfung. Dennoch konnte weder aus den Nasentupfern noch aus den Blutproben BHV-1-Impfvirus isoliert werden. Zudem konnte in den Blutproben keine BHV-1-spezifische DNA nachgewiesen werden. Aus diesen Ergebnissen l\u00e4sst sich schlussfolgern, dass das verwendete BHV-1-Impfvirus nach intramuskul\u00e4rer Applikation nicht mit dem Nasensekret ausgeschieden wurde und es nicht zur nachweisbaren Vir\u00e4mie in den Impflingen gekommen ist. Um die Ausscheidung von attenuiertem BHV-1-Impfvirus zu vermeiden, sollte die genannte attenuierte BHV-1 Markervakzine intramuskul\u00e4r verabreicht werden.<\/p>","primaryLanguage":"englisch","summary":"It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and\/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted.","zusammenfassung":"Mit Hilfe der hier vorgestellten Untersuchung sollte festgestellt werden, ob Rinder nach intramuskul\u00e4rer (IM) Impfung mit einer attenuierten Bovinen Herpesvirus Typ 1 (BHV-1)-Vakzine eine Vir\u00e4mie zeigen und\/oder Impfvirus mit dem Nasensekret ausscheiden. F\u00fcnf BHV-1-seronegative Rinder wurden hierf\u00fcr IM mit einer \u00dcberdosierung des Impfstoffes (BovilisR IBR marker live) immunisiert. Nasentupfer und Blutproben wurden in festgelegten Intervallen entnommen und im BHV-1-Virusnachweis getestet. Zus\u00e4tzlich wurde eine sensitive, f\u00fcr BHV-1-DNA spezifische, Polymerasekettenreaktion (PCR) mit Blutproben der inokulierten Tiere durchgef\u00fchrt. Die zun\u00e4chst BHV-1-negativen Tiere reagierten alle mit neutralisierenden Antik\u00f6rpern auf die Impfung. Dennoch konnte weder aus den Nasentupfern noch aus den Blutproben BHV-1-Impfvirus isoliert werden. Zudem konnte in den Blutproben keine BHV-1-spezifische DNA nachgewiesen werden. Aus diesen Ergebnissen l\u00e4sst sich schlussfolgern, dass das verwendete BHV-1-Impfvirus nach intramuskul\u00e4rer Applikation nicht mit dem Nasensekret ausgeschieden wurde und es nicht zur nachweisbaren Vir\u00e4mie in den Impflingen gekommen ist. Um die Ausscheidung von attenuiertem BHV-1-Impfvirus zu vermeiden, sollte die genannte attenuierte BHV-1 Markervakzine intramuskul\u00e4r verabreicht werden.<\/p>","translatedTitle":"DOI 10.2376\/0005-9366-120-480","abstractE":"It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and\/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted.","date":{"year":2007,"date":"11\/2007","accepted":"2007-11-01"},"volume":"120","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"480-482","redirects":["21593703\/150\/3130\/71153"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 11/2007 DO - 10.2376/0005-9366-120-480 LA - English N2 - It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2007 SP - 480 EP - 482 T1 - A live Bovine Herpesvirus-1 marker vaccine is not shed after intramuscular vaccination T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - A live Bovine Herpesvirus-1 marker vaccine is not shed after intramuscular vaccination TT - DOI 10.2376/0005-9366-120-480 VL - 120 SN - 0005-9366 ER -