TY - JOUR KW - antibodies KW - neutrophils KW - selectin KW - testis KW - torsion AU - M Celebi AU - AU - A Paul AB - Germ cell specific apoptosis after ischemia-reperfusion (I/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 ± 4 vs. 52 ± 10% Gr #150;1+CD11b+ of total leucocytes; P lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70723,"title":"Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury","teaserText":"antibodies, neutrophils, selectin, testis, torsion, Antik\u00f6rper, Neutrophile, Selectin, Hoden, Torsion","content":"

Summary<\/span>
Germ cell specific apoptosis after ischemia-reperfusion (I\/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I\/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I\/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 \u00b1 4 vs. 52 \u00b1 10% Gr #150;1+CD11b+ of total leucocytes; P lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. <\/p>

Keywords:<\/span> antibodies, neutrophils, selectin, testis, torsion<\/p>

Zusammenfassung<\/span>
Die Keimzell-spezifische Apoptose der Isch\u00e4mie-Reperfusion (I\/R) des Hodens ist von der Rekrutierung der Neutrophilen abh\u00e4ngig. Intravaskul\u00e4re Adh\u00e4sionsmolek\u00fcle wie P- und E-Selectin spielen eine wichtige Rolle in dieser Rekrutierung. Der Zweck dieser Studie war, die Neutrophilenrekrutierung, induziert durch I\/R der Hoden zu hemmen, indem ein funktionsblockierender monoklonaler Anti-Maus-P-Selectin Antik\u00f6rper verwendet wurde. Erwachsene M\u00e4use wurden f\u00fcr die Dauer von 2 Stunden einer Hodentorsion ausgesetzt, gefolgt von einer Detorsion (Reperfusion). 10 Minuten nach Beginn der Reperfusion wurde den M\u00e4usen entweder 100 amp;#956;g eines funktionsblockierenden monoklonalen P-Selectin Antik\u00f6rpers (FBMAB Gruppe) oder ein Isotyp-\u00e4hnlicher Steuerantik\u00f6rper appliziert (IMCA Gruppe). Weitere Gruppen von M\u00e4usen wurden einer vorget\u00e4uschten-OP unterzogen oder erhielten 500 ng TNF-alpha (IF Gruppe), um eine Entz\u00fcndung zu induzieren. Die M\u00e4use wurden 24 Stunden nach der Reperfusion euthanasiert, interstitielle Zellen wurden isoliert und anschliessend auf das Vorhandsein von Neutrophilen mittels Fluss-Zytometrie gepr\u00fcft. Die funktionsblockierenden monoklonalen P-Selectin Antik\u00f6rper haben die IR-induzierte Neutrophilenrekrutierung signifikant verringert.(FBMAB Gruppe verglichen mit der IMCA Gruppe 26 \u00b1 4 gegen. 52 \u00b1 10 % Gr-1+CD11b+ der Gesamtleukozyten; P lt; 0,001). Folglich ist die Blockade des P-Selectins eine vorteilhafte Therapie, um postischaemische Hoden zu sch\u00fctzen.<\/p>

Schl\u00fcsselw\u00f6rter:<\/span> Antik\u00f6rper, Neutrophile, Selectin, Hoden, Torsion<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Dec 1, 2008 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/antibodies-neutrophils-selectin-testis-torsion\/150\/3130\/70723"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"M. & H. Schaper GmbH","doiSerialWorkTitle":"Dtsch.tier\u00e4rztl.Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/antibodies-neutrophils-selectin-testis-torsion\/150\/3130\/70723","doiSource":"Dtsch.tier\u00e4rztl.Wschr. 115: 12, 457-460 (2008)","doiissn":"0341-6593","doiNr":"10.2376\/0341-6593-115-457","doiFirstPage":"457","doiLastPage":"460","doiTransmitted":true,"doiAuthor":"M. Celebi1,2, A. G. A. Paul3","pdf":{"path":"http:\/\/data\/dtw_2008_12_0457.pdf","title":"dtw_2008_12_0457.pdf","description":"Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury

"},"authors":[{"firstName":"M","middleName":"","lastName":"Celebi1"},{"firstName":"2","middleName":"","lastName":""},{"firstName":"A","middleName":"G.A.","lastName":"Paul3"}],"contentOptimised":"

Summary<\/strong>
Germ cell specific apoptosis after ischemia-reperfusion (I\/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I\/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I\/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 \u00b1 4 vs. 52 \u00b1 10% Gr #150;1+CD11b+ of total leucocytes; P lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. <\/p>

Keywords:<\/strong> antibodies, neutrophils, selectin, testis, torsion<\/p>

Zusammenfassung<\/strong>
Die Keimzell-spezifische Apoptose der Isch\u00e4mie-Reperfusion (I\/R) des Hodens ist von der Rekrutierung der Neutrophilen abh\u00e4ngig. Intravaskul\u00e4re Adh\u00e4sionsmolek\u00fcle wie P- und E-Selectin spielen eine wichtige Rolle in dieser Rekrutierung. Der Zweck dieser Studie war, die Neutrophilenrekrutierung, induziert durch I\/R der Hoden zu hemmen, indem ein funktionsblockierender monoklonaler Anti-Maus-P-Selectin Antik\u00f6rper verwendet wurde. Erwachsene M\u00e4use wurden f\u00fcr die Dauer von 2 Stunden einer Hodentorsion ausgesetzt, gefolgt von einer Detorsion (Reperfusion). 10 Minuten nach Beginn der Reperfusion wurde den M\u00e4usen entweder 100 amp;#956;g eines funktionsblockierenden monoklonalen P-Selectin Antik\u00f6rpers (FBMAB Gruppe) oder ein Isotyp-\u00e4hnlicher Steuerantik\u00f6rper appliziert (IMCA Gruppe). Weitere Gruppen von M\u00e4usen wurden einer vorget\u00e4uschten-OP unterzogen oder erhielten 500 ng TNF-alpha (IF Gruppe), um eine Entz\u00fcndung zu induzieren. Die M\u00e4use wurden 24 Stunden nach der Reperfusion euthanasiert, interstitielle Zellen wurden isoliert und anschliessend auf das Vorhandsein von Neutrophilen mittels Fluss-Zytometrie gepr\u00fcft. Die funktionsblockierenden monoklonalen P-Selectin Antik\u00f6rper haben die IR-induzierte Neutrophilenrekrutierung signifikant verringert.(FBMAB Gruppe verglichen mit der IMCA Gruppe 26 \u00b1 4 gegen. 52 \u00b1 10 % Gr-1+CD11b+ der Gesamtleukozyten; P lt; 0,001). Folglich ist die Blockade des P-Selectins eine vorteilhafte Therapie, um postischaemische Hoden zu sch\u00fctzen.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong> Antik\u00f6rper, Neutrophile, Selectin, Hoden, Torsion<\/p>","primaryLanguage":"englisch","summary":"Germ cell specific apoptosis after ischemia-reperfusion (I\/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I\/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I\/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 \u00b1 4 vs. 52 \u00b1 10% Gr #150;1+CD11b+ of total leucocytes; P lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. <\/p>

","keywords":["antibodies","neutrophils","selectin","testis","torsion"],"zusammenfassung":"Die Keimzell-spezifische Apoptose der Isch\u00e4mie-Reperfusion (I\/R) des Hodens ist von der Rekrutierung der Neutrophilen abh\u00e4ngig. Intravaskul\u00e4re Adh\u00e4sionsmolek\u00fcle wie P- und E-Selectin spielen eine wichtige Rolle in dieser Rekrutierung. Der Zweck dieser Studie war, die Neutrophilenrekrutierung, induziert durch I\/R der Hoden zu hemmen, indem ein funktionsblockierender monoklonaler Anti-Maus-P-Selectin Antik\u00f6rper verwendet wurde. Erwachsene M\u00e4use wurden f\u00fcr die Dauer von 2 Stunden einer Hodentorsion ausgesetzt, gefolgt von einer Detorsion (Reperfusion). 10 Minuten nach Beginn der Reperfusion wurde den M\u00e4usen entweder 100 amp;#956;g eines funktionsblockierenden monoklonalen P-Selectin Antik\u00f6rpers (FBMAB Gruppe) oder ein Isotyp-\u00e4hnlicher Steuerantik\u00f6rper appliziert (IMCA Gruppe). Weitere Gruppen von M\u00e4usen wurden einer vorget\u00e4uschten-OP unterzogen oder erhielten 500 ng TNF-alpha (IF Gruppe), um eine Entz\u00fcndung zu induzieren. Die M\u00e4use wurden 24 Stunden nach der Reperfusion euthanasiert, interstitielle Zellen wurden isoliert und anschliessend auf das Vorhandsein von Neutrophilen mittels Fluss-Zytometrie gepr\u00fcft. Die funktionsblockierenden monoklonalen P-Selectin Antik\u00f6rper haben die IR-induzierte Neutrophilenrekrutierung signifikant verringert.(FBMAB Gruppe verglichen mit der IMCA Gruppe 26 \u00b1 4 gegen. 52 \u00b1 10 % Gr-1+CD11b+ der Gesamtleukozyten; P lt; 0,001). Folglich ist die Blockade des P-Selectins eine vorteilhafte Therapie, um postischaemische Hoden zu sch\u00fctzen.<\/p>

","schluesselwoerter":["Antik\u00f6rper","Neutrophile","Selectin","Hoden","Torsion"],"translatedTitle":"antibodies, neutrophils, selectin, testis, torsion, Antik\u00f6rper, Neutrophile, Selectin, Hoden, Torsion","abstractE":"Germ cell specific apoptosis after ischemia-reperfusion (I\/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I\/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I\/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 \u00b1 4 vs. 52 \u00b1 10% Gr #150;1+CD11b+ of total leucocytes; P lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. ","date":{"year":2008,"date":"12\/2008","accepted":"2008-12-01"},"volume":"115","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"457-460","redirects":["antibodies-neutrophils-selectin-testis-torsion\/150\/3130\/70723"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 12/2008 DO - 10.2376/0341-6593-115-457 LA - English N2 - Germ cell specific apoptosis after ischemia-reperfusion (I/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 ± 4 vs. 52 ± 10% Gr #150;1+CD11b+ of total leucocytes; P lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. PB - M. & H. Schaper GmbH PP - Hannover PY - 2008 SP - 457 EP - 460 T1 - Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury TT - antibodies, neutrophils, selectin, testis, torsion, Antikörper, Neutrophile, Selectin, Hoden, Torsion VL - 115 SN - 0341-6593 ER -