TY - JOUR KW - next generation sequencing KW - deep sequencing KW - rabies KW - defective interfering particle AU - D Höper AU - S Finke AU - C Freuling AU - B Hoffmann AU - M Beer AB - The main task of the individual project number four #147;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; within the network #147;Lyssaviruses #150; a potential re-emerging public health threat #148; is to provide high quality complete genome sequences from lyssaviruses. These sequences are analysed in-depth with regard to the diversity of the viral populations as to both quasi-species and so-called defective interfering RNAs. Moreover, the sequence data will facilitate further epidemiological analyses, will provide insight into the evolution of lyssaviruses and will be the basis for the design of novel nucleic acid based diagnostics. The first results presented here indicate that not only high quality full-length lyssavirus genome sequences can be generated, but indeed efficient analysis of the viral population gets feasible. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70644,"title":"What can we learn about lyssavirus","topline":"","teaserText":"","content":"

Summary<\/span>
The main task of the individual project number four #147;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; within the network #147;Lyssaviruses #150; a potential re-emerging public health threat #148; is to provide high quality complete genome sequences from lyssaviruses. These sequences are analysed in-depth with regard to the diversity of the viral populations as to both quasi-species and so-called defective interfering RNAs. Moreover, the sequence data will facilitate further epidemiological analyses, will provide insight into the evolution of lyssaviruses and will be the basis for the design of novel nucleic acid based diagnostics. The first results presented here indicate that not only high quality full-length lyssavirus genome sequences can be generated, but indeed efficient analysis of the viral population gets feasible.

Keywords:<\/span>
next generation sequencing, deep sequencing, rabies, defective interfering particle


Zusammenfassung<\/span>
Hauptziel des Teilprojektes 4 mit dem Titel #132;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; innerhalb des Forschungsverbundes #132;Lyssaviruses #150; a potential re-emerging public health threat #147; ist es, vollst\u00e4ndige Lyssavirus-Genomsequenzen mit hoher Qualit\u00e4t zu erstellen. Diese Sequenzen werden im Hinblick auf die Zusammensetzung der Virenpopulation hinsichtlich Quasispezies und des Vorkommens sogenannter #132;defective intefering RNAs #147; detailliert untersucht. Dar\u00fcber hinaus werden die gewonnen Sequenzinformationen f\u00fcr epidemiologische Analysen herangezogen und Aussagen \u00fcber die Evolution der Lyssaviren erlauben. Weiterhin dienen die Genomsequenzen als Basis f\u00fcr die Entwicklung neuer Nukleins\u00e4ure-basierter Diagnostikmethoden. Die ersten hier dargestellten Ergebnisse des Projektes zeigen bereits, dass nicht nur die Gewinnung qualitativ hochwertiger Genomsequenzen, sondern auch die Analyse der Diversit\u00e4t der Viruspopulation m\u00f6glich ist.

Schl\u00fcsselw\u00f6rter:<\/span>
next generation sequencing, deep sequencing, Tollwut, defective interfering particle <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"May 4, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/next-generation-sequencing-deep-sequencing-rabies-defective-interfering-particle\/150\/3130\/70644","http:\/\/vetline.de\/next-generation-sequencing-deep-sequencing-rabies-defective-interfering-particle\/150\/3216\/70644"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/next-generation-sequencing-deep-sequencing-rabies-defective-interfering-particle\/150\/3130\/70644","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 5-6, 242-248 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-242","doiFirstPage":"242","doiLastPage":"248","doiTransmitted":true,"doiAuthor":"H\u00f6per D, Finke S, Freuling CM, Hoffmann B, Beer M","pdf":{"path":"http:\/\/data\/bmtw_2012_05_0242.pdf","title":"bmtw - What can we learn about lyssavirus ","description":""},"authors":[{"firstName":"D","middleName":"","lastName":"H\u00f6per"},{"firstName":"S","middleName":"","lastName":"Finke"},{"firstName":"C","middleName":"M","lastName":"Freuling"},{"firstName":"B","middleName":"","lastName":"Hoffmann"},{"firstName":"M","middleName":"","lastName":"Beer"}],"contentOptimised":"

Summary<\/strong>
The main task of the individual project number four #147;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; within the network #147;Lyssaviruses #150; a potential re-emerging public health threat #148; is to provide high quality complete genome sequences from lyssaviruses. These sequences are analysed in-depth with regard to the diversity of the viral populations as to both quasi-species and so-called defective interfering RNAs. Moreover, the sequence data will facilitate further epidemiological analyses, will provide insight into the evolution of lyssaviruses and will be the basis for the design of novel nucleic acid based diagnostics. The first results presented here indicate that not only high quality full-length lyssavirus genome sequences can be generated, but indeed efficient analysis of the viral population gets feasible.

Keywords:<\/strong>
next generation sequencing, deep sequencing, rabies, defective interfering particle


Zusammenfassung<\/strong>
Hauptziel des Teilprojektes 4 mit dem Titel #132;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; innerhalb des Forschungsverbundes #132;Lyssaviruses #150; a potential re-emerging public health threat #147; ist es, vollst\u00e4ndige Lyssavirus-Genomsequenzen mit hoher Qualit\u00e4t zu erstellen. Diese Sequenzen werden im Hinblick auf die Zusammensetzung der Virenpopulation hinsichtlich Quasispezies und des Vorkommens sogenannter #132;defective intefering RNAs #147; detailliert untersucht. Dar\u00fcber hinaus werden die gewonnen Sequenzinformationen f\u00fcr epidemiologische Analysen herangezogen und Aussagen \u00fcber die Evolution der Lyssaviren erlauben. Weiterhin dienen die Genomsequenzen als Basis f\u00fcr die Entwicklung neuer Nukleins\u00e4ure-basierter Diagnostikmethoden. Die ersten hier dargestellten Ergebnisse des Projektes zeigen bereits, dass nicht nur die Gewinnung qualitativ hochwertiger Genomsequenzen, sondern auch die Analyse der Diversit\u00e4t der Viruspopulation m\u00f6glich ist.

Schl\u00fcsselw\u00f6rter:<\/strong>
next generation sequencing, deep sequencing, Tollwut, defective interfering particle <\/p>","primaryLanguage":"englisch","summary":"The main task of the individual project number four #147;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; within the network #147;Lyssaviruses #150; a potential re-emerging public health threat #148; is to provide high quality complete genome sequences from lyssaviruses. These sequences are analysed in-depth with regard to the diversity of the viral populations as to both quasi-species and so-called defective interfering RNAs. Moreover, the sequence data will facilitate further epidemiological analyses, will provide insight into the evolution of lyssaviruses and will be the basis for the design of novel nucleic acid based diagnostics. The first results presented here indicate that not only high quality full-length lyssavirus genome sequences can be generated, but indeed efficient analysis of the viral population gets feasible.","keywords":["next generation sequencing","deep sequencing","rabies","defective interfering particle"],"zusammenfassung":"Hauptziel des Teilprojektes 4 mit dem Titel #132;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; innerhalb des Forschungsverbundes #132;Lyssaviruses #150; a potential re-emerging public health threat #147; ist es, vollst\u00e4ndige Lyssavirus-Genomsequenzen mit hoher Qualit\u00e4t zu erstellen. Diese Sequenzen werden im Hinblick auf die Zusammensetzung der Virenpopulation hinsichtlich Quasispezies und des Vorkommens sogenannter #132;defective intefering RNAs #147; detailliert untersucht. Dar\u00fcber hinaus werden die gewonnen Sequenzinformationen f\u00fcr epidemiologische Analysen herangezogen und Aussagen \u00fcber die Evolution der Lyssaviren erlauben. Weiterhin dienen die Genomsequenzen als Basis f\u00fcr die Entwicklung neuer Nukleins\u00e4ure-basierter Diagnostikmethoden. Die ersten hier dargestellten Ergebnisse des Projektes zeigen bereits, dass nicht nur die Gewinnung qualitativ hochwertiger Genomsequenzen, sondern auch die Analyse der Diversit\u00e4t der Viruspopulation m\u00f6glich ist.","schluesselwoerter":["next generation sequencing","deep sequencing","Tollwut","defective interfering particle"],"translatedTitle":"","abstractE":"The main task of the individual project number four #147;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; within the network #147;Lyssaviruses #150; a potential re-emerging public health threat #148; is to provide high quality complete genome sequences from lyssaviruses. These sequences are analysed in-depth with regard to the diversity of the viral populations as to both quasi-species and so-called defective interfering RNAs. Moreover, the sequence data will facilitate further epidemiological analyses, will provide insight into the evolution of lyssaviruses and will be the basis for the design of novel nucleic acid based diagnostics. The first results presented here indicate that not only high quality full-length lyssavirus genome sequences can be generated, but indeed efficient analysis of the viral population gets feasible.","date":{"year":2012,"date":"05\/2012","accepted":"2012-05-04"},"volume":"125","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"242-248","redirects":["next-generation-sequencing-deep-sequencing-rabies-defective-interfering-particle\/150\/3130\/70644","next-generation-sequencing-deep-sequencing-rabies-defective-interfering-particle\/150\/3216\/70644"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 05/2012 DO - 10.2376/0005-9366-125-242 LA - English N2 - The main task of the individual project number four #147;Whole genome sequencing, virus-host adaptation, and molecular epidemiological analyses of lyssaviruses #147; within the network #147;Lyssaviruses #150; a potential re-emerging public health threat #148; is to provide high quality complete genome sequences from lyssaviruses. These sequences are analysed in-depth with regard to the diversity of the viral populations as to both quasi-species and so-called defective interfering RNAs. Moreover, the sequence data will facilitate further epidemiological analyses, will provide insight into the evolution of lyssaviruses and will be the basis for the design of novel nucleic acid based diagnostics. The first results presented here indicate that not only high quality full-length lyssavirus genome sequences can be generated, but indeed efficient analysis of the viral population gets feasible. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2012 SP - 242 EP - 248 T1 - What can we learn about lyssavirus T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - What can we learn about lyssavirus VL - 125 SN - 0005-9366 ER -