TY - JOUR KW - Salmonella AU - U Roesler AU - I Szabo AU - C Matthies AU - K Albrecht AU - M Leffler AU - K Scherer AU - K Nöckler AU - J Lehmann AU - U Methner AU - A Hensel AU - U Truyen AB - The objective of this study was the comparative evaluation of four indirect Salmonella ELISA tests at study time approved in Germany to detect Salmonella infection in pigs. Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella-specific IgM-, IgA-, and IgG- antibodies. In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs. Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derbyinfected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70636,"title":"Comparing validation of four ELISAsystems for detection of Salmonella Derby- and S almonella Infantis-infected pigs","topline":"","teaserText":"","content":"

Summary<\/span>
The objective of this study was the comparative evaluation of four indirect Salmonella<\/span> ELISA tests at study time approved in Germany to detect Salmonella<\/span> infection in pigs. Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella<\/span>-specific IgM-, IgA-, and IgG- antibodies.

In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs.

Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella<\/span> Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella<\/span>-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derbyinfected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates.

Keywords:<\/span>
Salmonella<\/span>, Derby, Infantis, pig, ELISA, validation


Zusammenfassung<\/span>
Gegenstand dieser Studie war die vergleichende Evaluierung von vier zum Studienzeitpunkt in Deutschland f\u00fcr die serologische Diagnostik von Salmonella<\/span>- Infektionen des Schweins zugelassenen ELISA-Testsystemen.

Zum Einsatz kamen drei LPS-basierte ELISA-Systeme zur Detektion von spezifischen IgG-Antik\u00f6rpern sowie ein auf einem S.-Typhimurium-Vollzelllysat-Antigen- basierender ELISA, welcher zwischen Salmonella<\/span>-spezifischen IgM-, IgA-, und IgG-Antik\u00f6rpern unterscheiden kann.

Im Rahmen einer Langzeitstudie wurden zwei Gruppen von jeweils sechs Wochen alten Hybridschweinen mit Bakterien der Salmonella<\/span>-Serovare S. Infantis und S. Derby oral infiziert. Nach der Infektion wurden \u00fcber einen Zeitraum von 130 Tagen w\u00f6chentlich sowohl klinische als auch bakteriologische Parameter erfasst. Parallel wurden Blutproben mit den jeweiligen ELISA-Testsystemen untersucht. Es zeigte sich, dass mit den in dieser Studie verwendeten LPS-basierten ELISA-Systemen keine mit S. Infantis infizierten Schweine erkannt werden konnten, obwohl die Tiere den Erreger bis zum Tag 81 p. i. in gro\u00dfen Mengen ausschieden. Dagegen war der S.-Typhimurium-Vollzelllysat-basierte ELISA zehn Tage p. i. in der Lage, die meisten dieser Tiere Serovar-unabh\u00e4ngig als Salmonellen-infiziert zu identifizieren und wies zudem die h\u00f6chste Sensitivit\u00e4t bez\u00fcglich des Nachweises von S.-Infantis-infzierten Tieren auf.

Weiterhin erwies sich der in Deutschland angewandte Surveillance cut-off-Wert von 40 OD% als nicht geeignet f\u00fcr die Detektion von mit S. Infantis und S. Derby infizierten Schweinen. Im Gegensatz dazu f\u00fchrte die Anwendung der von den Hersteller-Firmen vorgeschlagenen cut-off-Werte der ELISA-Testsysteme zu einer deutlich h\u00f6heren Test-Sensitivit\u00e4t.

Schl\u00fcsselw\u00f6rter:<\/span>
Salmonella<\/span>, Derby, Infantis, Schwein, ELISA, Validierung <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Jun 21, 2011 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/open-access-salmonella-derby-infantis-pig-elisa-validation\/150\/3216\/70636","http:\/\/vetline.de\/open-access-salmonella-derby-infantis-pig-elisa-validation\/150\/3130\/70636"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/open-access-salmonella-derby-infantis-pig-elisa-validation\/150\/3130\/70636","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 124: 7-8, 265-271 (2011)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-124-265","doiFirstPage":"265","doiLastPage":"271","doiTransmitted":true,"doiAuthor":"Roesler U, Szabo1 I, Matthies C, Albrecht K, Leffler M, Scherer K, N\u00f6ckler K, Lehmann J, Methner U, Hensel A, Truyen U","pdf":{"path":"http:\/\/data\/bmtw_2011_07_0265.pdf","title":"bmtw - Comparing validation of four ELISAsystems for detection of Salmonella Derby- and S almonella Infantis-infected pigs ","description":""},"authors":[{"firstName":"U","middleName":"","lastName":"Roesler"},{"firstName":"I","middleName":"","lastName":"Szabo1"},{"firstName":"C","middleName":"","lastName":"Matthies"},{"firstName":"K","middleName":"","lastName":"Albrecht"},{"firstName":"M","middleName":"","lastName":"Leffler"},{"firstName":"K","middleName":"","lastName":"Scherer"},{"firstName":"K","middleName":"","lastName":"N\u00f6ckler"},{"firstName":"J","middleName":"","lastName":"Lehmann"},{"firstName":"U","middleName":"","lastName":"Methner"},{"firstName":"A","middleName":"","lastName":"Hensel"},{"firstName":"U","middleName":"","lastName":"Truyen"}],"contentOptimised":"

Summary<\/strong>
The objective of this study was the comparative evaluation of four indirect Salmonella<\/em> ELISA tests at study time approved in Germany to detect Salmonella<\/em> infection in pigs. Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella<\/em>-specific IgM-, IgA-, and IgG- antibodies.

In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs.

Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella<\/em> Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella<\/em>-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derbyinfected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates.

Keywords:<\/strong>
Salmonella<\/em>, Derby, Infantis, pig, ELISA, validation


Zusammenfassung<\/strong>
Gegenstand dieser Studie war die vergleichende Evaluierung von vier zum Studienzeitpunkt in Deutschland f\u00fcr die serologische Diagnostik von Salmonella<\/em>- Infektionen des Schweins zugelassenen ELISA-Testsystemen.

Zum Einsatz kamen drei LPS-basierte ELISA-Systeme zur Detektion von spezifischen IgG-Antik\u00f6rpern sowie ein auf einem S.-Typhimurium-Vollzelllysat-Antigen- basierender ELISA, welcher zwischen Salmonella<\/em>-spezifischen IgM-, IgA-, und IgG-Antik\u00f6rpern unterscheiden kann.

Im Rahmen einer Langzeitstudie wurden zwei Gruppen von jeweils sechs Wochen alten Hybridschweinen mit Bakterien der Salmonella<\/em>-Serovare S. Infantis und S. Derby oral infiziert. Nach der Infektion wurden \u00fcber einen Zeitraum von 130 Tagen w\u00f6chentlich sowohl klinische als auch bakteriologische Parameter erfasst. Parallel wurden Blutproben mit den jeweiligen ELISA-Testsystemen untersucht. Es zeigte sich, dass mit den in dieser Studie verwendeten LPS-basierten ELISA-Systemen keine mit S. Infantis infizierten Schweine erkannt werden konnten, obwohl die Tiere den Erreger bis zum Tag 81 p. i. in gro\u00dfen Mengen ausschieden. Dagegen war der S.-Typhimurium-Vollzelllysat-basierte ELISA zehn Tage p. i. in der Lage, die meisten dieser Tiere Serovar-unabh\u00e4ngig als Salmonellen-infiziert zu identifizieren und wies zudem die h\u00f6chste Sensitivit\u00e4t bez\u00fcglich des Nachweises von S.-Infantis-infzierten Tieren auf.

Weiterhin erwies sich der in Deutschland angewandte Surveillance cut-off-Wert von 40 OD% als nicht geeignet f\u00fcr die Detektion von mit S. Infantis und S. Derby infizierten Schweinen. Im Gegensatz dazu f\u00fchrte die Anwendung der von den Hersteller-Firmen vorgeschlagenen cut-off-Werte der ELISA-Testsysteme zu einer deutlich h\u00f6heren Test-Sensitivit\u00e4t.

Schl\u00fcsselw\u00f6rter:<\/strong>
Salmonella<\/em>, Derby, Infantis, Schwein, ELISA, Validierung <\/p>","primaryLanguage":"englisch","summary":"The objective of this study was the comparative evaluation of four indirect Salmonella<\/em> ELISA tests at study time approved in Germany to detect Salmonella<\/em> infection in pigs. Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella<\/em>-specific IgM-, IgA-, and IgG- antibodies.

In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs.

Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella<\/em> Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella<\/em>-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derbyinfected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates.","keywords":["Salmonella"],"zusammenfassung":"Gegenstand dieser Studie war die vergleichende Evaluierung von vier zum Studienzeitpunkt in Deutschland f\u00fcr die serologische Diagnostik von Salmonella<\/em>- Infektionen des Schweins zugelassenen ELISA-Testsystemen.

Zum Einsatz kamen drei LPS-basierte ELISA-Systeme zur Detektion von spezifischen IgG-Antik\u00f6rpern sowie ein auf einem S.-Typhimurium-Vollzelllysat-Antigen- basierender ELISA, welcher zwischen Salmonella<\/em>-spezifischen IgM-, IgA-, und IgG-Antik\u00f6rpern unterscheiden kann.

Im Rahmen einer Langzeitstudie wurden zwei Gruppen von jeweils sechs Wochen alten Hybridschweinen mit Bakterien der Salmonella<\/em>-Serovare S. Infantis und S. Derby oral infiziert. Nach der Infektion wurden \u00fcber einen Zeitraum von 130 Tagen w\u00f6chentlich sowohl klinische als auch bakteriologische Parameter erfasst. Parallel wurden Blutproben mit den jeweiligen ELISA-Testsystemen untersucht. Es zeigte sich, dass mit den in dieser Studie verwendeten LPS-basierten ELISA-Systemen keine mit S. Infantis infizierten Schweine erkannt werden konnten, obwohl die Tiere den Erreger bis zum Tag 81 p. i. in gro\u00dfen Mengen ausschieden. Dagegen war der S.-Typhimurium-Vollzelllysat-basierte ELISA zehn Tage p. i. in der Lage, die meisten dieser Tiere Serovar-unabh\u00e4ngig als Salmonellen-infiziert zu identifizieren und wies zudem die h\u00f6chste Sensitivit\u00e4t bez\u00fcglich des Nachweises von S.-Infantis-infzierten Tieren auf.

Weiterhin erwies sich der in Deutschland angewandte Surveillance cut-off-Wert von 40 OD% als nicht geeignet f\u00fcr die Detektion von mit S. Infantis und S. Derby infizierten Schweinen. Im Gegensatz dazu f\u00fchrte die Anwendung der von den Hersteller-Firmen vorgeschlagenen cut-off-Werte der ELISA-Testsysteme zu einer deutlich h\u00f6heren Test-Sensitivit\u00e4t.","schluesselwoerter":["Salmonella"],"translatedTitle":"","abstractE":"The objective of this study was the comparative evaluation of four indirect Salmonella ELISA tests at study time approved in Germany to detect Salmonella infection in pigs. Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella-specific IgM-, IgA-, and IgG- antibodies. In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs. Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derbyinfected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates.","date":{"year":2011,"date":"06\/2011","accepted":"2011-06-21"},"volume":"124","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"265-271","redirects":["open-access-salmonella-derby-infantis-pig-elisa-validation\/150\/3216\/70636","open-access-salmonella-derby-infantis-pig-elisa-validation\/150\/3130\/70636"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 06/2011 DO - 10.2376/0005-9366-124-265 LA - English N2 - The objective of this study was the comparative evaluation of four indirect Salmonella ELISA tests at study time approved in Germany to detect Salmonella infection in pigs. Three tests are based on a LPS-antigen mix and directed against specific IgG antibodies. The fourth test is based on a purified S. Typhimurium whole-cell lysate antigen and discriminates between Salmonella-specific IgM-, IgA-, and IgG- antibodies. In a longitudinal study, two groups of six weeks old hybrid piglets were orally infected with a porcine S. Infantis or S. Derby strain. Clinical and bacteriological parameters were monitored weekly during an observation period of 130 days after infection and serum samples were investigated in parallel with the respective ELISAs. Apparently, the LPS-based ELISA systems used in this study failed to recognize S. Infantis-infected pigs although those animals shed the pathogen in high amounts throughout the study until day 81 post infection (p. i.). In contrast, the isotype-specific Salmonella Typhimurium whole-cell-lysate based ELISA was capable of detecting Salmonella-infected pigs from day ten p. i. at all tested serotypes and revealed the highest sensitivity in detection of S. Infantis-infected pigs. Furthermore, it became apparent that the often used surveillance cut-off value of 40 OD% is not appropriate for intra-vitam detection of S. Infantis- and S. Derbyinfected pigs. In contrast, the cut-off values of the ELISAs given by the suppliers result in considerable higher detection rates. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2011 SP - 265 EP - 271 T1 - Comparing validation of four ELISAsystems for detection of Salmonella Derby- and S almonella Infantis-infected pigs T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Comparing validation of four ELISAsystems for detection of Salmonella Derby- and S almonella Infantis-infected pigs VL - 124 SN - 0005-9366 ER -