TY - JOUR KW - susceptibility testing KW - resistance pattern KW - husbandry system KW - antibiotic treatment KW - genotyping AU - V Buntenkoetter AU - T Blaha AU - R Tegeler AU - A Fetsch AU - M Hartmann AU - L Kreienbrock AU - D Meemken AB - The purposes of this study were to identify differences in the phenotypic resistance to antimicrobials and in the spa-types between 273 methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from conventional (n = 21) versus organic pig farms (n = 10) located in Germany. The susceptibility of the isolates against 19 antimicrobial agents was tested and then compared between the two different husbandry systems. A statistically significant difference was observed between the MRSA strains isolated on conventional and on organic pig farms for the antimicrobials tilmicosin (61.8% vs. 40.0%; OR: 2.42), clindamycin (63.5% vs. 45.7%; OR: 2.06), gentamicin (14.7% vs. 34.3%; OR: 0.33), apramycin (3.8% vs. 22.9%; OR: 0.13) and enrofloxacin (13.9% vs. 34.3%; OR: 0.31). Finally, the results of the susceptibility testing were analysed in order to determine the resistance pattern per isolate. Among the tested isolates a kind of “basic resistance pattern of MRSA” to penicillin, ampicillin and tetracycline was identified. The predominant spa-types in both groups were t011 and t034. Less frequently detected spa-types were t1430, t1197, t2510, t779, t1451 and t1250. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":78736,"title":"Comparison of the phenotypic antimicrobial resistances and spa-types of methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from pigs in conventional and in organic husbandry systems","topline":"","teaserText":"Vergleich der ph\u00e4notypischen Antibiotikaresistenz von Methicillin- resistenten Staphylococcus aureus(MRSA)-St\u00e4mmen, isoliert von Schweinen aus konventionellen und \u00f6kologischen Haltungssystemen","content":"

Summary<\/span>
The purposes of this study were to identify differences in the phenotypic resistance to antimicrobials and in the spa<\/span>-types between 273 methicillin-resistant Staphylococcus aureus <\/span>(MRSA) isolates derived from conventional (n = 21) versus organic pig farms (n = 10) located in Germany. The susceptibility of the isolates against 19 antimicrobial agents was tested and then compared between the two different husbandry systems. A statistically significant difference was observed between the MRSA strains isolated on conventional and on organic pig farms for the antimicrobials tilmicosin (61.8% vs. 40.0%; OR: 2.42), clindamycin (63.5% vs. 45.7%; OR: 2.06), gentamicin (14.7% vs. 34.3%; OR: 0.33), apramycin (3.8% vs. 22.9%; OR: 0.13) and enrofloxacin (13.9% vs. 34.3%; OR: 0.31). Finally, the results of the susceptibility testing were analysed in order to determine the resistance pattern per isolate. Among the tested isolates a kind of \u201cbasic resistance pattern of MRSA\u201d to penicillin, ampicillin and tetracycline was identified. The predominant spa<\/span>-types in both groups were t011 and t034. Less frequently detected spa<\/span>-types were t1430, t1197, t2510, t779, t1451 and t1250. <\/p>

Keywords<\/span>
susceptibility testing, resistance pattern, husbandry system, antibiotic treatment, genotyping <\/p>

Zusammenfassung<\/span>
Ziel der Studie ist die Identifizierung von Unterschieden in der ph\u00e4notypischen, antimikrobiellen Resistenz und in den Genotypen von 273 Methicillin-resistenten Staphylococcus aureus<\/span>(MRSA)-St\u00e4mmen. Die St\u00e4mme wurden in konventionell wirtschaftenden (n = 21) und \u00f6kologisch wirtschaftenden (n = 10) Schweinebetrieben in Deutschland isoliert. Das Resistenzverhalten der Isolate aus den beiden Haltungssystemen wurde gegen\u00fcber 19 Antibiotika getestet und verglichen. Hierbei konnten statistisch signifikante Unterschiede f\u00fcr Tilmicosin (61,8 % vs. 40,0 %; OR: 2.42), Clindamycin (63,5 % vs. 45,7 %; OR: 2,06), Gentamycin (14,7 % vs. 34,3 %; OR: 0,33), Apramycin (3,8 % vs. 22,9 %; OR: 0,13) und Enrofloxacin (13,9 % vs. 34,3 %; OR: 0,31) festgestellt werden. Au\u00dferdem sind die Ergebnisse der Resistenzteste zus\u00e4tzlich als Resistenzmuster pro Isolat analysiert worden. So konnte bei den untersuchten St\u00e4mmen eine Art \u201eMinimum-Resistenzmuster von MRSA\u201c gegen Penicillin, Ampicillin und Tetracyclin festgestellt werden. Die am h\u00e4ufigsten vorkommenden spa<\/span>-Typen in beiden Gruppen waren t011 und t034. Au\u00dferdem wurden noch Isolate den spa<\/span>-Typen t1430, t1197, t2510, t779, t1451 und t1250 zugeordnet. <\/p>

Schl\u00fcsselworter<\/span>
Resistenztestung, Resistenzmuster, Haltungssystem, antibiotische Behandlung, Genotypisierung<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"May 9, 2014 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/comparison-of-the-phenotypic-antimicrobial-resistances-and-spa-types-of-methicillin-resistant-staphylococcus-aureus-mrsa-isolates-derived-from-pigs-in-conventional-and-in-organic-husbandry-systems\/150\/3130\/78736"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/vetline.de\/index.cfm?documents.id=78736&cid=3130","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 127, 135\u2013143 (2014)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-127-135","doiFirstPage":"135","doiLastPage":"143","doiTransmitted":true,"doiAuthor":"Buntenkoetter V, Blaha T, Tegeler R, Fetsch A, Hartmann M, Kreienbrock L, Meemken D","pdf":{"path":"http:\/\/data\/BMTW_2014_03_0135.pdf.pdf","title":"BMTW_2014_03_0135.pdf","description":"Comparison of the phenotypic antimicrobial resistances and spa-types of methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from pigs in conventional and in organic husbandry systems"},"authors":[{"firstName":"V","middleName":"","lastName":"Buntenkoetter"},{"firstName":"T","middleName":"","lastName":"Blaha"},{"firstName":"R","middleName":"","lastName":"Tegeler"},{"firstName":"A","middleName":"","lastName":"Fetsch"},{"firstName":"M","middleName":"","lastName":"Hartmann"},{"firstName":"L","middleName":"","lastName":"Kreienbrock"},{"firstName":"D","middleName":"","lastName":"Meemken"}],"contentOptimised":"

Summary<\/strong>
The purposes of this study were to identify differences in the phenotypic resistance to antimicrobials and in the spa<\/em>-types between 273 methicillin-resistant Staphylococcus aureus <\/em>(MRSA) isolates derived from conventional (n = 21) versus organic pig farms (n = 10) located in Germany. The susceptibility of the isolates against 19 antimicrobial agents was tested and then compared between the two different husbandry systems. A statistically significant difference was observed between the MRSA strains isolated on conventional and on organic pig farms for the antimicrobials tilmicosin (61.8% vs. 40.0%; OR: 2.42), clindamycin (63.5% vs. 45.7%; OR: 2.06), gentamicin (14.7% vs. 34.3%; OR: 0.33), apramycin (3.8% vs. 22.9%; OR: 0.13) and enrofloxacin (13.9% vs. 34.3%; OR: 0.31). Finally, the results of the susceptibility testing were analysed in order to determine the resistance pattern per isolate. Among the tested isolates a kind of \u201cbasic resistance pattern of MRSA\u201d to penicillin, ampicillin and tetracycline was identified. The predominant spa<\/em>-types in both groups were t011 and t034. Less frequently detected spa<\/em>-types were t1430, t1197, t2510, t779, t1451 and t1250. <\/p>

Keywords:<\/strong>
susceptibility testing, resistance pattern, husbandry system, antibiotic treatment, genotyping <\/p>

Zusammenfassung<\/strong>
Ziel der Studie ist die Identifizierung von Unterschieden in der ph\u00e4notypischen, antimikrobiellen Resistenz und in den Genotypen von 273 Methicillin-resistenten Staphylococcus aureus<\/em>(MRSA)-St\u00e4mmen. Die St\u00e4mme wurden in konventionell wirtschaftenden (n = 21) und \u00f6kologisch wirtschaftenden (n = 10) Schweinebetrieben in Deutschland isoliert. Das Resistenzverhalten der Isolate aus den beiden Haltungssystemen wurde gegen\u00fcber 19 Antibiotika getestet und verglichen. Hierbei konnten statistisch signifikante Unterschiede f\u00fcr Tilmicosin (61,8 % vs. 40,0 %; OR: 2.42), Clindamycin (63,5 % vs. 45,7 %; OR: 2,06), Gentamycin (14,7 % vs. 34,3 %; OR: 0,33), Apramycin (3,8 % vs. 22,9 %; OR: 0,13) und Enrofloxacin (13,9 % vs. 34,3 %; OR: 0,31) festgestellt werden. Au\u00dferdem sind die Ergebnisse der Resistenzteste zus\u00e4tzlich als Resistenzmuster pro Isolat analysiert worden. So konnte bei den untersuchten St\u00e4mmen eine Art \u201eMinimum-Resistenzmuster von MRSA\u201c gegen Penicillin, Ampicillin und Tetracyclin festgestellt werden. Die am h\u00e4ufigsten vorkommenden spa<\/em>-Typen in beiden Gruppen waren t011 und t034. Au\u00dferdem wurden noch Isolate den spa<\/em>-Typen t1430, t1197, t2510, t779, t1451 und t1250 zugeordnet. <\/p>

Schl\u00fcsselworter<\/strong>
Resistenztestung, Resistenzmuster, Haltungssystem, antibiotische Behandlung, Genotypisierung<\/p>","primaryLanguage":"englisch","summary":"The purposes of this study were to identify differences in the phenotypic resistance to antimicrobials and in the spa<\/em>-types between 273 methicillin-resistant Staphylococcus aureus <\/em>(MRSA) isolates derived from conventional (n = 21) versus organic pig farms (n = 10) located in Germany. The susceptibility of the isolates against 19 antimicrobial agents was tested and then compared between the two different husbandry systems. A statistically significant difference was observed between the MRSA strains isolated on conventional and on organic pig farms for the antimicrobials tilmicosin (61.8% vs. 40.0%; OR: 2.42), clindamycin (63.5% vs. 45.7%; OR: 2.06), gentamicin (14.7% vs. 34.3%; OR: 0.33), apramycin (3.8% vs. 22.9%; OR: 0.13) and enrofloxacin (13.9% vs. 34.3%; OR: 0.31). Finally, the results of the susceptibility testing were analysed in order to determine the resistance pattern per isolate. Among the tested isolates a kind of \u201cbasic resistance pattern of MRSA\u201d to penicillin, ampicillin and tetracycline was identified. The predominant spa<\/em>-types in both groups were t011 and t034. Less frequently detected spa<\/em>-types were t1430, t1197, t2510, t779, t1451 and t1250. <\/p>

","keywords":["susceptibility testing","resistance pattern","husbandry system","antibiotic treatment","genotyping"],"zusammenfassung":"Ziel der Studie ist die Identifizierung von Unterschieden in der ph\u00e4notypischen, antimikrobiellen Resistenz und in den Genotypen von 273 Methicillin-resistenten Staphylococcus aureus<\/em>(MRSA)-St\u00e4mmen. Die St\u00e4mme wurden in konventionell wirtschaftenden (n = 21) und \u00f6kologisch wirtschaftenden (n = 10) Schweinebetrieben in Deutschland isoliert. Das Resistenzverhalten der Isolate aus den beiden Haltungssystemen wurde gegen\u00fcber 19 Antibiotika getestet und verglichen. Hierbei konnten statistisch signifikante Unterschiede f\u00fcr Tilmicosin (61,8 % vs. 40,0 %; OR: 2.42), Clindamycin (63,5 % vs. 45,7 %; OR: 2,06), Gentamycin (14,7 % vs. 34,3 %; OR: 0,33), Apramycin (3,8 % vs. 22,9 %; OR: 0,13) und Enrofloxacin (13,9 % vs. 34,3 %; OR: 0,31) festgestellt werden. Au\u00dferdem sind die Ergebnisse der Resistenzteste zus\u00e4tzlich als Resistenzmuster pro Isolat analysiert worden. So konnte bei den untersuchten St\u00e4mmen eine Art \u201eMinimum-Resistenzmuster von MRSA\u201c gegen Penicillin, Ampicillin und Tetracyclin festgestellt werden. Die am h\u00e4ufigsten vorkommenden spa<\/em>-Typen in beiden Gruppen waren t011 und t034. Au\u00dferdem wurden noch Isolate den spa<\/em>-Typen t1430, t1197, t2510, t779, t1451 und t1250 zugeordnet. <\/p>

Schl\u00fcsselworter<\/strong>
Resistenztestung, Resistenzmuster, Haltungssystem, antibiotische Behandlung, Genotypisierung<\/p>","translatedTitle":"Vergleich der ph\u00e4notypischen Antibiotikaresistenz von Methicillin- resistenten Staphylococcus aureus(MRSA)-St\u00e4mmen, isoliert von Schweinen aus konventionellen und \u00f6kologischen Haltungssystemen","abstractE":"The purposes of this study were to identify differences in the phenotypic resistance to antimicrobials and in the spa-types between 273 methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from conventional (n = 21) versus organic pig farms (n = 10) located in Germany. The susceptibility of the isolates against 19 antimicrobial agents was tested and then compared between the two different husbandry systems. A statistically significant difference was observed between the MRSA strains isolated on conventional and on organic pig farms for the antimicrobials tilmicosin (61.8% vs. 40.0%; OR: 2.42), clindamycin (63.5% vs. 45.7%; OR: 2.06), gentamicin (14.7% vs. 34.3%; OR: 0.33), apramycin (3.8% vs. 22.9%; OR: 0.13) and enrofloxacin (13.9% vs. 34.3%; OR: 0.31). Finally, the results of the susceptibility testing were analysed in order to determine the resistance pattern per isolate. Among the tested isolates a kind of \u201cbasic resistance pattern of MRSA\u201d to penicillin, ampicillin and tetracycline was identified. The predominant spa-types in both groups were t011 and t034. Less frequently detected spa-types were t1430, t1197, t2510, t779, t1451 and t1250. ","date":{"year":2014,"date":"05\/2014","accepted":"2014-05-09"},"volume":"127","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"135-143","redirects":["comparison-of-the-phenotypic-antimicrobial-resistances-and-spa-types-of-methicillin-resistant-staphylococcus-aureus-mrsa-isolates-derived-from-pigs-in-conventional-and-in-organic-husbandry-systems\/150\/3130\/78736"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 05/2014 DO - 10.2376/0005-9366-127-135 LA - English N2 - The purposes of this study were to identify differences in the phenotypic resistance to antimicrobials and in the spa-types between 273 methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from conventional (n = 21) versus organic pig farms (n = 10) located in Germany. The susceptibility of the isolates against 19 antimicrobial agents was tested and then compared between the two different husbandry systems. A statistically significant difference was observed between the MRSA strains isolated on conventional and on organic pig farms for the antimicrobials tilmicosin (61.8% vs. 40.0%; OR: 2.42), clindamycin (63.5% vs. 45.7%; OR: 2.06), gentamicin (14.7% vs. 34.3%; OR: 0.33), apramycin (3.8% vs. 22.9%; OR: 0.13) and enrofloxacin (13.9% vs. 34.3%; OR: 0.31). Finally, the results of the susceptibility testing were analysed in order to determine the resistance pattern per isolate. Among the tested isolates a kind of “basic resistance pattern of MRSA” to penicillin, ampicillin and tetracycline was identified. The predominant spa-types in both groups were t011 and t034. Less frequently detected spa-types were t1430, t1197, t2510, t779, t1451 and t1250. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2014 SP - 135 EP - 143 T1 - Comparison of the phenotypic antimicrobial resistances and spa-types of methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from pigs in conventional and in organic husbandry systems T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Comparison of the phenotypic antimicrobial resistances and spa-types of methicillin-resistant Staphylococcus aureus (MRSA) isolates derived from pigs in conventional and in organic husbandry systems TT - Vergleich der phänotypischen Antibiotikaresistenz von Methicillin- resistenten Staphylococcus aureus(MRSA)-Stämmen, isoliert von Schweinen aus konventionellen und ökologischen Haltungssystemen VL - 127 SN - 0005-9366 ER -