TY - JOUR AU - Z Ilhan AU - H Solmaz AU - A Aksakal AU - T Gülhan AU - I Ekin AU - B Boynukara AB - The aim of this study was to evaluate the polymerase chain reaction (PCR) assayfor detection of Brucella melitensis in stomach content samples of aborted sheepfetuses and to compare its performance with bacteriological culture method.It was also aimed to determine the agreement between PCR and Rose Bengalplate test (RBPT). Materials were collected from aborted sheep from 109 differentsheep flocks in the region of Van during the lambing seasons of 2004–2005 and2005–2006. Stomach contents from 135 aborted sheep fetuses were examinedby bacteriological culture and PCR, and 135 sera from these aborting ewes weretested by RBPT. Identification and typing of Brucella strains were performed usingstandard classification test. B. melitensis biovar 3 was isolated from 26 (19.2 %) offoetal stomach contents. B. melitensis was detected by PCR in 29 (21.4 %) stomachcontent samples. Twenty five sera (18.5 %) from aborting ewes tested positiveby RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 103 cfu(colony forming units) /ml in spiked stomach contents. Diagnostic sensitivity andspecificity of the PCR were detected as 100 % and 97.2 %, respectively. The agreementbetween PCR and RBPT was found to be 97 %. In conclusion, PCR assaywould have an advantage over conventional bacteriological culture method, butin particular for its ability to meet the specificity requirements for the detection ofB. melitensis in stomach content samples of aborted sheep fetuses. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70540,"title":"Comparison of PCR assay and bacteriological culture method for the detection of Brucella melitensis in stomach content samples of aborted sheep fetuses","teaserText":"Brucella melitensis, sheep, PCR, bacteriological culture, RBPT, Brucella melitensis, Schafe, PCR, bakteriologische Kultur, RBPT","content":"

Summary<\/span>
The aim of this study was to evaluate the polymerase chain reaction (PCR) assayfor detection of Brucella melitensis in stomach content samples of aborted sheepfetuses and to compare its performance with bacteriological culture method.It was also aimed to determine the agreement between PCR and Rose Bengalplate test (RBPT). Materials were collected from aborted sheep from 109 differentsheep flocks in the region of Van during the lambing seasons of 2004\u20132005 and2005\u20132006. Stomach contents from 135 aborted sheep fetuses were examinedby bacteriological culture and PCR, and 135 sera from these aborting ewes weretested by RBPT. Identification and typing of Brucella strains were performed usingstandard classification test. B. melitensis biovar 3 was isolated from 26 (19.2 %) offoetal stomach contents. B. melitensis was detected by PCR in 29 (21.4 %) stomachcontent samples. Twenty five sera (18.5 %) from aborting ewes tested positiveby RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 103 cfu(colony forming units) \/ml in spiked stomach contents. Diagnostic sensitivity andspecificity of the PCR were detected as 100 % and 97.2 %, respectively. The agreementbetween PCR and RBPT was found to be 97 %. In conclusion, PCR assaywould have an advantage over conventional bacteriological culture method, butin particular for its ability to meet the specificity requirements for the detection ofB. melitensis in stomach content samples of aborted sheep fetuses.


Zusammenfassung<\/span>
Das Ziel dieser Studie war, Ergebnisse der Polymerase Kettenreaktion (PCR) aufBrucella melitensis im Mageninhalt abortierter Schaffeten auszuwerten und mitden Ergebnissen der bakteriologischen Kulturmethode zu vergleichen bzw. die\u00dcbereinstimmung zwischen PCR und Rose Bengal Test (RBPT) zu \u00fcberpr\u00fcfen.Abortierte Feten aus der Region Van aus 109 unterschiedlichen Schaf-Herdengelangten w\u00e4hrend der Lammzeiten von 2004\u20132005 und von 2005\u20132006 zurUntersuchung. Der Mageninhalt von 135 abortierten Schaffeten wurden kulturellsowie mittels PCR untersucht. Von 135 M\u00fcttern mit Aborten wurden Blutprobenmittels RBPT serologisch untersucht. Die kulturelle Untersuchung und Typisierungenwurden mit Standardklassifikationtests durchgef\u00fchrt und B. melitensisbiovar 3 wurde im Mageninhalt von 26 (19.2 %) der Proben nachgewiesen.B. melitensis wurde mittels PCR in 29 (21.4 %) Proben des Mageninhalts ermittelt.25 Seren (18.5 %) reagierten im RBPT positiv. Die Nachweisgrenze des aufB. melitensis 16 M Stammes mittels PCR war 1.7 x 103 Kolonie bildende Einheiten(KBE)\/ml im gespikten Mageninhalt. Sensitivit\u00e4t und Spezifit\u00e4t der PCR wurdenmit 100 % bzw. 97.2 % ermittelt. Die \u00dcbereinstimmung zwischen PCR und RBPT lagbei 97 %. Die PCR ist aufgrund dieser Ergebnisse der kulturellen Untersuchung\u00fcberlegen, insbesondere aufgrund der hohen Spezifit\u00e4t beim Nachweis vonB. melitensis im Mageninhalt abortierter Feten.<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Dec 1, 2007 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/brucella-melitensis-sheep-pcr-bacteriological-culture-rbpt\/150\/3130\/70540"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"M. & H. Schaper GmbH","doiSerialWorkTitle":"Dtsch.tier\u00e4rztl.Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/brucella-melitensis-sheep-pcr-bacteriological-culture-rbpt\/150\/3130\/70540","doiSource":"Dtsch.tier\u00e4rztl.Wschr. 114: 12, 460-464 (2007)","doiissn":"0341-6593","doiNr":"10.2377\/0341-6593-114-460","doiFirstPage":"460","doiLastPage":"464","doiTransmitted":true,"doiAuthor":"\u00ddLHAN Z, SOLMAZ H, AKSAKAL A, G\u00dcLHAN T, EK\u00ddN \u00dd H, BOYNUKARA B","pdf":{"path":"http:\/\/data\/dtw_2007_12_0460.pdf","title":"dtw_2007_12_0460.pdf","description":"Comparison of PCR assay and bacteriological culture method for the detection of Brucella melitensis in stomach content samples of aborted sheep fetuses

"},"authors":[{"firstName":"\u00dd","middleName":"LHANZ","lastName":""},{"firstName":"S","middleName":"OLMAZH","lastName":""},{"firstName":"A","middleName":"KSAKALA","lastName":""},{"firstName":"G","middleName":"\u00dcLHANT","lastName":""},{"firstName":"E","middleName":"K\u00ddN\u00ddH","lastName":""},{"firstName":"B","middleName":"OYNUKARAB","lastName":""}],"contentOptimised":"

Summary<\/strong>
The aim of this study was to evaluate the polymerase chain reaction (PCR) assayfor detection of Brucella melitensis in stomach content samples of aborted sheepfetuses and to compare its performance with bacteriological culture method.It was also aimed to determine the agreement between PCR and Rose Bengalplate test (RBPT). Materials were collected from aborted sheep from 109 differentsheep flocks in the region of Van during the lambing seasons of 2004\u20132005 and2005\u20132006. Stomach contents from 135 aborted sheep fetuses were examinedby bacteriological culture and PCR, and 135 sera from these aborting ewes weretested by RBPT. Identification and typing of Brucella strains were performed usingstandard classification test. B. melitensis biovar 3 was isolated from 26 (19.2 %) offoetal stomach contents. B. melitensis was detected by PCR in 29 (21.4 %) stomachcontent samples. Twenty five sera (18.5 %) from aborting ewes tested positiveby RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 103 cfu(colony forming units) \/ml in spiked stomach contents. Diagnostic sensitivity andspecificity of the PCR were detected as 100 % and 97.2 %, respectively. The agreementbetween PCR and RBPT was found to be 97 %. In conclusion, PCR assaywould have an advantage over conventional bacteriological culture method, butin particular for its ability to meet the specificity requirements for the detection ofB. melitensis in stomach content samples of aborted sheep fetuses.


Zusammenfassung<\/strong>
Das Ziel dieser Studie war, Ergebnisse der Polymerase Kettenreaktion (PCR) aufBrucella melitensis im Mageninhalt abortierter Schaffeten auszuwerten und mitden Ergebnissen der bakteriologischen Kulturmethode zu vergleichen bzw. die\u00dcbereinstimmung zwischen PCR und Rose Bengal Test (RBPT) zu \u00fcberpr\u00fcfen.Abortierte Feten aus der Region Van aus 109 unterschiedlichen Schaf-Herdengelangten w\u00e4hrend der Lammzeiten von 2004\u20132005 und von 2005\u20132006 zurUntersuchung. Der Mageninhalt von 135 abortierten Schaffeten wurden kulturellsowie mittels PCR untersucht. Von 135 M\u00fcttern mit Aborten wurden Blutprobenmittels RBPT serologisch untersucht. Die kulturelle Untersuchung und Typisierungenwurden mit Standardklassifikationtests durchgef\u00fchrt und B. melitensisbiovar 3 wurde im Mageninhalt von 26 (19.2 %) der Proben nachgewiesen.B. melitensis wurde mittels PCR in 29 (21.4 %) Proben des Mageninhalts ermittelt.25 Seren (18.5 %) reagierten im RBPT positiv. Die Nachweisgrenze des aufB. melitensis 16 M Stammes mittels PCR war 1.7 x 103 Kolonie bildende Einheiten(KBE)\/ml im gespikten Mageninhalt. Sensitivit\u00e4t und Spezifit\u00e4t der PCR wurdenmit 100 % bzw. 97.2 % ermittelt. Die \u00dcbereinstimmung zwischen PCR und RBPT lagbei 97 %. Die PCR ist aufgrund dieser Ergebnisse der kulturellen Untersuchung\u00fcberlegen, insbesondere aufgrund der hohen Spezifit\u00e4t beim Nachweis vonB. melitensis im Mageninhalt abortierter Feten.<\/p>","primaryLanguage":"englisch","summary":"The aim of this study was to evaluate the polymerase chain reaction (PCR) assayfor detection of Brucella melitensis in stomach content samples of aborted sheepfetuses and to compare its performance with bacteriological culture method.It was also aimed to determine the agreement between PCR and Rose Bengalplate test (RBPT). Materials were collected from aborted sheep from 109 differentsheep flocks in the region of Van during the lambing seasons of 2004\u20132005 and2005\u20132006. Stomach contents from 135 aborted sheep fetuses were examinedby bacteriological culture and PCR, and 135 sera from these aborting ewes weretested by RBPT. Identification and typing of Brucella strains were performed usingstandard classification test. B. melitensis biovar 3 was isolated from 26 (19.2 %) offoetal stomach contents. B. melitensis was detected by PCR in 29 (21.4 %) stomachcontent samples. Twenty five sera (18.5 %) from aborting ewes tested positiveby RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 103 cfu(colony forming units) \/ml in spiked stomach contents. Diagnostic sensitivity andspecificity of the PCR were detected as 100 % and 97.2 %, respectively. The agreementbetween PCR and RBPT was found to be 97 %. In conclusion, PCR assaywould have an advantage over conventional bacteriological culture method, butin particular for its ability to meet the specificity requirements for the detection ofB. melitensis in stomach content samples of aborted sheep fetuses.","zusammenfassung":"Das Ziel dieser Studie war, Ergebnisse der Polymerase Kettenreaktion (PCR) aufBrucella melitensis im Mageninhalt abortierter Schaffeten auszuwerten und mitden Ergebnissen der bakteriologischen Kulturmethode zu vergleichen bzw. die\u00dcbereinstimmung zwischen PCR und Rose Bengal Test (RBPT) zu \u00fcberpr\u00fcfen.Abortierte Feten aus der Region Van aus 109 unterschiedlichen Schaf-Herdengelangten w\u00e4hrend der Lammzeiten von 2004\u20132005 und von 2005\u20132006 zurUntersuchung. Der Mageninhalt von 135 abortierten Schaffeten wurden kulturellsowie mittels PCR untersucht. Von 135 M\u00fcttern mit Aborten wurden Blutprobenmittels RBPT serologisch untersucht. Die kulturelle Untersuchung und Typisierungenwurden mit Standardklassifikationtests durchgef\u00fchrt und B. melitensisbiovar 3 wurde im Mageninhalt von 26 (19.2 %) der Proben nachgewiesen.B. melitensis wurde mittels PCR in 29 (21.4 %) Proben des Mageninhalts ermittelt.25 Seren (18.5 %) reagierten im RBPT positiv. Die Nachweisgrenze des aufB. melitensis 16 M Stammes mittels PCR war 1.7 x 103 Kolonie bildende Einheiten(KBE)\/ml im gespikten Mageninhalt. Sensitivit\u00e4t und Spezifit\u00e4t der PCR wurdenmit 100 % bzw. 97.2 % ermittelt. Die \u00dcbereinstimmung zwischen PCR und RBPT lagbei 97 %. Die PCR ist aufgrund dieser Ergebnisse der kulturellen Untersuchung\u00fcberlegen, insbesondere aufgrund der hohen Spezifit\u00e4t beim Nachweis vonB. melitensis im Mageninhalt abortierter Feten.<\/p>","translatedTitle":"Brucella melitensis, sheep, PCR, bacteriological culture, RBPT, Brucella melitensis, Schafe, PCR, bakteriologische Kultur, RBPT","abstractE":"The aim of this study was to evaluate the polymerase chain reaction (PCR) assayfor detection of Brucella melitensis in stomach content samples of aborted sheepfetuses and to compare its performance with bacteriological culture method.It was also aimed to determine the agreement between PCR and Rose Bengalplate test (RBPT). Materials were collected from aborted sheep from 109 differentsheep flocks in the region of Van during the lambing seasons of 2004\u20132005 and2005\u20132006. Stomach contents from 135 aborted sheep fetuses were examinedby bacteriological culture and PCR, and 135 sera from these aborting ewes weretested by RBPT. Identification and typing of Brucella strains were performed usingstandard classification test. B. melitensis biovar 3 was isolated from 26 (19.2 %) offoetal stomach contents. B. melitensis was detected by PCR in 29 (21.4 %) stomachcontent samples. Twenty five sera (18.5 %) from aborting ewes tested positiveby RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 103 cfu(colony forming units) \/ml in spiked stomach contents. Diagnostic sensitivity andspecificity of the PCR were detected as 100 % and 97.2 %, respectively. The agreementbetween PCR and RBPT was found to be 97 %. In conclusion, PCR assaywould have an advantage over conventional bacteriological culture method, butin particular for its ability to meet the specificity requirements for the detection ofB. melitensis in stomach content samples of aborted sheep fetuses.","date":{"year":2007,"date":"12\/2007","accepted":"2007-12-01"},"volume":"114","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"460-464","redirects":["brucella-melitensis-sheep-pcr-bacteriological-culture-rbpt\/150\/3130\/70540"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 12/2007 DO - 10.2377/0341-6593-114-460 LA - English N2 - The aim of this study was to evaluate the polymerase chain reaction (PCR) assayfor detection of Brucella melitensis in stomach content samples of aborted sheepfetuses and to compare its performance with bacteriological culture method.It was also aimed to determine the agreement between PCR and Rose Bengalplate test (RBPT). Materials were collected from aborted sheep from 109 differentsheep flocks in the region of Van during the lambing seasons of 2004–2005 and2005–2006. Stomach contents from 135 aborted sheep fetuses were examinedby bacteriological culture and PCR, and 135 sera from these aborting ewes weretested by RBPT. Identification and typing of Brucella strains were performed usingstandard classification test. B. melitensis biovar 3 was isolated from 26 (19.2 %) offoetal stomach contents. B. melitensis was detected by PCR in 29 (21.4 %) stomachcontent samples. Twenty five sera (18.5 %) from aborting ewes tested positiveby RBPT. The detection limit of B. melitensis 16 M strain by PCR was 1.7 x 103 cfu(colony forming units) /ml in spiked stomach contents. Diagnostic sensitivity andspecificity of the PCR were detected as 100 % and 97.2 %, respectively. The agreementbetween PCR and RBPT was found to be 97 %. In conclusion, PCR assaywould have an advantage over conventional bacteriological culture method, butin particular for its ability to meet the specificity requirements for the detection ofB. melitensis in stomach content samples of aborted sheep fetuses. PB - M. & H. Schaper GmbH PP - Hannover PY - 2007 SP - 460 EP - 464 T1 - Comparison of PCR assay and bacteriological culture method for the detection of Brucella melitensis in stomach content samples of aborted sheep fetuses T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Comparison of PCR assay and bacteriological culture method for the detection of Brucella melitensis in stomach content samples of aborted sheep fetuses TT - Brucella melitensis, sheep, PCR, bacteriological culture, RBPT, Brucella melitensis, Schafe, PCR, bakteriologische Kultur, RBPT VL - 114 SN - 0341-6593 ER -