TY - JOUR KW - methicillin-resistant Staphylococcus pseudintermedius AU - S Vincze AU - A Paasch AU - B Walther AU - C Ruscher AU - A Lübke-Becker AU - L Wieler AU - B Kohn AB - A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round. Comparative typing of all MRSP-isolates by pulsed-field gel electrophoresis (PFGE), SCCmec typing, multilocus sequence typing (MLST), spa typing, PCRdetection of the leukotoxin encoding operon (LukI) and the Staphylococcus intermedius-exfoliative toxin (SIET) as well as antimicrobial resistance profiling by broth microdilution revealed that all five MRSP isolates from the dog and the single isolate from the owner were indistinguishable by any of the applied methods. All isolates were assigned to a certain strain, a multidrug-resistant MRSP belonging to sequence type (ST) 71, spa type (t)05, harbouring SCCmecIII as well as the genes encoding LukI and SIET. In this case, a number of reasons might have contributed to therapy failure and re-infection, respectively (e. g. contact to other MRSP-colonized dogs, contact to MRSP-colonized humans, refusal to clip the dog`s fur). In addition, MRSP-contaminated objects or surfaces in the household, which were difficult to disinfect or simply not considered as a potential source of MRSP, might have served as a source of re-infection. These results envision the possibility of a dog-to-human transmission of MRSP and the relevance of this aspect as a potential source of re-infection in cases of bacterial-supported long-term skin disorders in canine patients. First cases of MRSP infections in humans have been described only recently. However, the general pathogenic potential of multidrug resistant MRSP in humans is unknown so far and needs further investigation. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70513,"title":"Multidrug- and methicillin resistant Staphylococcus pseudintermedius as a cause of canine pyoderma: a case report","topline":"","teaserText":"","content":"

Summary<\/span>
A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/span> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/span> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/span> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.
Comparative typing of all MRSP-isolates by pulsed-field gel electrophoresis (PFGE), SCCmec<\/span> typing, multilocus sequence typing (MLST), spa<\/span> typing, PCRdetection of the leukotoxin encoding operon (LukI) and the Staphylococcus intermedius<\/span>-exfoliative toxin (SIET) as well as antimicrobial resistance profiling by broth microdilution revealed that all five MRSP isolates from the dog and the single isolate from the owner were indistinguishable by any of the applied methods. All isolates were assigned to a certain strain, a multidrug-resistant MRSP belonging to sequence type (ST) 71, spa type (t)05, harbouring SCCmec<\/span>III as well as the genes encoding LukI and SIET.
In this case, a number of reasons might have contributed to therapy failure and re-infection, respectively (e. g. contact to other MRSP-colonized dogs, contact to MRSP-colonized humans, refusal to clip the dog`s fur). In addition, MRSP-contaminated objects or surfaces in the household, which were difficult to disinfect or simply not considered as a potential source of MRSP, might have served as a source of re-infection.
These results envision the possibility of a dog-to-human transmission of MRSP and the relevance of this aspect as a potential source of re-infection in cases of bacterial-supported long-term skin disorders in canine patients. First cases of MRSP infections in humans have been described only recently. However, the general pathogenic potential of multidrug resistant MRSP in humans is unknown so far and needs further investigation.

Keywords:<\/span>
methicillin-resistant Staphylococcus pseudintermedius<\/span>, MRSP, pyoderma, dog-to-human transmission


Zusammenfassung<\/span>
Es wird der Fall eines Hundes mit chronischer Hautentz\u00fcndung beschrieben, die durch eine Infektion mit Methicillin-resistenten Staphylococcus pseudintermedius<\/span> (MRSP) hervorgerufen wurde. Nach dem ersten mikrobiologischen Nachweis von MRSP in einer Hauttupferprobe wurde noch zweimal (nach vier und sieben Wochen) sowohl die Haut als auch die Hundenase mittels Abstrich untersucht. Von der Haut konnte MRSP konstant isoliert werden, w\u00e4hrend von den beiden Nasentupfern nur einer positiv war. Da unter anderem auch kolonisierte Besitzer eine m\u00f6gliche Quelle f\u00fcr erneute Infektionen sein k\u00f6nnten, wurden beide Besitzer insgesamt dreimal im Laufe der Therapie ihres Tieres nasal auf MRSP untersucht. In der ersten Tupferprobe des m\u00e4nnlichen Besitzers konnte MRSP nachgewiesen werden.
Die MRSP-Isolate wurden durch Pulsfeld-Gelelektrophorese, SCCmec<\/span>-Typisierung, Multilokus Sequenztypisierung, spa-Typisierung sowie PCR zum Nachweis der Gene f\u00fcr Leukotoxin (LukI) und das Staphylococcus intermedius<\/span>-exfoliative Toxin (SIET) charakterisiert. Des Weiteren wurde f\u00fcr die untersuchten Isolate ein Resistenzprofil f\u00fcr nicht-\u00df-Lactam-Antiinfektiva erstellt. Mit den vorgenannten Typisierungsmethoden konnten identische Ergebnisse f\u00fcr alle f\u00fcnf caninen sowie f\u00fcr das eine humane MRSP-Isolat ermittelt werden, sodass alle Isolate dem gleichen Stamm zuzuordnen waren: ein multiresistenter MRSP vom Sequenztyp (ST) 71, SCCmec<\/span>III, spa Typ (t)05), der sich zudem in der PCR positiv f\u00fcr die LukI- und SIETkodierenden Gene zeigte.
Grunds\u00e4tzlich kommen f\u00fcr ein Therapieversagen bzw. f\u00fcr eine Reinfektion zahlreiche Ursachen in Betracht, z. B. der Kontakt zu asymptomatisch besiedelten anderen Hunden oder Menschen, unzureichend desinfizierten oder nur schwer desinfizierbaren Gegenst\u00e4nden oder Fl\u00e4chen im h\u00e4uslichen Bereich sowie die nicht durchgef\u00fchrte Schur des Hundes.
Dennoch deuten diese Ergebnisse auf eine grunds\u00e4tzlich m\u00f6gliche \u00dcbertragbarkeit von MRSP zwischen Hund und Mensch hin und lassen Raum f\u00fcr eine Diskussion \u00fcber die Relevanz von Besitzern als potentielle Reinfektionsquelle f\u00fcr Tiere mit bakteriell bedingten chronischen Hautentz\u00fcndungen. Dar\u00fcber hinaus wurden erst k\u00fcrzlich MRSP-Infektionen beim Menschen beschrieben, jedoch ist das pathogene Potential von multiresistenten MRSP f\u00fcr Menschen bislang unbekannt.

Schl\u00fcsselw\u00f6rter:<\/span>
Methicillin-resistente Staphylococcus pseudintermedius<\/span>, MRSP, Pyodermie, Hund-zu-Mensch-\u00dcbertragung <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Aug 20, 2010 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/open-access-methicillin-resistant-staphylococcus-pseudintermedius-mrsp-pyoderma-dog-human-transmission\/150\/3216\/70513","http:\/\/vetline.de\/open-access-methicillin-resistant-staphylococcus-pseudintermedius-mrsp-pyoderma-dog-human-transmission\/150\/3130\/70513"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/open-access-methicillin-resistant-staphylococcus-pseudintermedius-mrsp-pyoderma-dog-human-transmission\/150\/3130\/70513","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 123: 9-10, 353-358 (2010)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-123-353","doiFirstPage":"353","doiLastPage":"358","doiTransmitted":true,"doiAuthor":"Vincze S, Paasch A, Walther B, Ruscher C, L\u00fcbke-Becker A, Wieler LH, Kohn B","pdf":{"path":"http:\/\/data\/bmtw_2010_09_0353.pdf","title":"bmtw - Multidrug- and methicillin resistant Staphylococcus pseudintermedius as a cause of canine pyoderma: a case report ","description":""},"authors":[{"firstName":"S","middleName":"","lastName":"Vincze"},{"firstName":"A","middleName":"","lastName":"Paasch"},{"firstName":"B","middleName":"","lastName":"Walther"},{"firstName":"C","middleName":"","lastName":"Ruscher"},{"firstName":"A","middleName":"","lastName":"L\u00fcbke-Becker"},{"firstName":"L","middleName":"H","lastName":"Wieler"},{"firstName":"B","middleName":"","lastName":"Kohn"}],"contentOptimised":"

Summary<\/strong>
A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/em> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/em> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/em> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.
Comparative typing of all MRSP-isolates by pulsed-field gel electrophoresis (PFGE), SCCmec<\/em> typing, multilocus sequence typing (MLST), spa<\/em> typing, PCRdetection of the leukotoxin encoding operon (LukI) and the Staphylococcus intermedius<\/em>-exfoliative toxin (SIET) as well as antimicrobial resistance profiling by broth microdilution revealed that all five MRSP isolates from the dog and the single isolate from the owner were indistinguishable by any of the applied methods. All isolates were assigned to a certain strain, a multidrug-resistant MRSP belonging to sequence type (ST) 71, spa type (t)05, harbouring SCCmec<\/em>III as well as the genes encoding LukI and SIET.
In this case, a number of reasons might have contributed to therapy failure and re-infection, respectively (e. g. contact to other MRSP-colonized dogs, contact to MRSP-colonized humans, refusal to clip the dog`s fur). In addition, MRSP-contaminated objects or surfaces in the household, which were difficult to disinfect or simply not considered as a potential source of MRSP, might have served as a source of re-infection.
These results envision the possibility of a dog-to-human transmission of MRSP and the relevance of this aspect as a potential source of re-infection in cases of bacterial-supported long-term skin disorders in canine patients. First cases of MRSP infections in humans have been described only recently. However, the general pathogenic potential of multidrug resistant MRSP in humans is unknown so far and needs further investigation.

Keywords:<\/strong>
methicillin-resistant Staphylococcus pseudintermedius<\/em>, MRSP, pyoderma, dog-to-human transmission


Zusammenfassung<\/strong>
Es wird der Fall eines Hundes mit chronischer Hautentz\u00fcndung beschrieben, die durch eine Infektion mit Methicillin-resistenten Staphylococcus pseudintermedius<\/em> (MRSP) hervorgerufen wurde. Nach dem ersten mikrobiologischen Nachweis von MRSP in einer Hauttupferprobe wurde noch zweimal (nach vier und sieben Wochen) sowohl die Haut als auch die Hundenase mittels Abstrich untersucht. Von der Haut konnte MRSP konstant isoliert werden, w\u00e4hrend von den beiden Nasentupfern nur einer positiv war. Da unter anderem auch kolonisierte Besitzer eine m\u00f6gliche Quelle f\u00fcr erneute Infektionen sein k\u00f6nnten, wurden beide Besitzer insgesamt dreimal im Laufe der Therapie ihres Tieres nasal auf MRSP untersucht. In der ersten Tupferprobe des m\u00e4nnlichen Besitzers konnte MRSP nachgewiesen werden.
Die MRSP-Isolate wurden durch Pulsfeld-Gelelektrophorese, SCCmec<\/em>-Typisierung, Multilokus Sequenztypisierung, spa-Typisierung sowie PCR zum Nachweis der Gene f\u00fcr Leukotoxin (LukI) und das Staphylococcus intermedius<\/em>-exfoliative Toxin (SIET) charakterisiert. Des Weiteren wurde f\u00fcr die untersuchten Isolate ein Resistenzprofil f\u00fcr nicht-\u00df-Lactam-Antiinfektiva erstellt. Mit den vorgenannten Typisierungsmethoden konnten identische Ergebnisse f\u00fcr alle f\u00fcnf caninen sowie f\u00fcr das eine humane MRSP-Isolat ermittelt werden, sodass alle Isolate dem gleichen Stamm zuzuordnen waren: ein multiresistenter MRSP vom Sequenztyp (ST) 71, SCCmec<\/em>III, spa Typ (t)05), der sich zudem in der PCR positiv f\u00fcr die LukI- und SIETkodierenden Gene zeigte.
Grunds\u00e4tzlich kommen f\u00fcr ein Therapieversagen bzw. f\u00fcr eine Reinfektion zahlreiche Ursachen in Betracht, z. B. der Kontakt zu asymptomatisch besiedelten anderen Hunden oder Menschen, unzureichend desinfizierten oder nur schwer desinfizierbaren Gegenst\u00e4nden oder Fl\u00e4chen im h\u00e4uslichen Bereich sowie die nicht durchgef\u00fchrte Schur des Hundes.
Dennoch deuten diese Ergebnisse auf eine grunds\u00e4tzlich m\u00f6gliche \u00dcbertragbarkeit von MRSP zwischen Hund und Mensch hin und lassen Raum f\u00fcr eine Diskussion \u00fcber die Relevanz von Besitzern als potentielle Reinfektionsquelle f\u00fcr Tiere mit bakteriell bedingten chronischen Hautentz\u00fcndungen. Dar\u00fcber hinaus wurden erst k\u00fcrzlich MRSP-Infektionen beim Menschen beschrieben, jedoch ist das pathogene Potential von multiresistenten MRSP f\u00fcr Menschen bislang unbekannt.

Schl\u00fcsselw\u00f6rter:<\/strong>
Methicillin-resistente Staphylococcus pseudintermedius<\/em>, MRSP, Pyodermie, Hund-zu-Mensch-\u00dcbertragung <\/p>","primaryLanguage":"englisch","summary":"A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/em> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/em> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius<\/em> (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.
Comparative typing of all MRSP-isolates by pulsed-field gel electrophoresis (PFGE), SCCmec<\/em> typing, multilocus sequence typing (MLST), spa<\/em> typing, PCRdetection of the leukotoxin encoding operon (LukI) and the Staphylococcus intermedius<\/em>-exfoliative toxin (SIET) as well as antimicrobial resistance profiling by broth microdilution revealed that all five MRSP isolates from the dog and the single isolate from the owner were indistinguishable by any of the applied methods. All isolates were assigned to a certain strain, a multidrug-resistant MRSP belonging to sequence type (ST) 71, spa type (t)05, harbouring SCCmec<\/em>III as well as the genes encoding LukI and SIET.
In this case, a number of reasons might have contributed to therapy failure and re-infection, respectively (e. g. contact to other MRSP-colonized dogs, contact to MRSP-colonized humans, refusal to clip the dog`s fur). In addition, MRSP-contaminated objects or surfaces in the household, which were difficult to disinfect or simply not considered as a potential source of MRSP, might have served as a source of re-infection.
These results envision the possibility of a dog-to-human transmission of MRSP and the relevance of this aspect as a potential source of re-infection in cases of bacterial-supported long-term skin disorders in canine patients. First cases of MRSP infections in humans have been described only recently. However, the general pathogenic potential of multidrug resistant MRSP in humans is unknown so far and needs further investigation.","keywords":["methicillin-resistant Staphylococcus pseudintermedius"],"zusammenfassung":"Es wird der Fall eines Hundes mit chronischer Hautentz\u00fcndung beschrieben, die durch eine Infektion mit Methicillin-resistenten Staphylococcus pseudintermedius<\/em> (MRSP) hervorgerufen wurde. Nach dem ersten mikrobiologischen Nachweis von MRSP in einer Hauttupferprobe wurde noch zweimal (nach vier und sieben Wochen) sowohl die Haut als auch die Hundenase mittels Abstrich untersucht. Von der Haut konnte MRSP konstant isoliert werden, w\u00e4hrend von den beiden Nasentupfern nur einer positiv war. Da unter anderem auch kolonisierte Besitzer eine m\u00f6gliche Quelle f\u00fcr erneute Infektionen sein k\u00f6nnten, wurden beide Besitzer insgesamt dreimal im Laufe der Therapie ihres Tieres nasal auf MRSP untersucht. In der ersten Tupferprobe des m\u00e4nnlichen Besitzers konnte MRSP nachgewiesen werden.
Die MRSP-Isolate wurden durch Pulsfeld-Gelelektrophorese, SCCmec<\/em>-Typisierung, Multilokus Sequenztypisierung, spa-Typisierung sowie PCR zum Nachweis der Gene f\u00fcr Leukotoxin (LukI) und das Staphylococcus intermedius<\/em>-exfoliative Toxin (SIET) charakterisiert. Des Weiteren wurde f\u00fcr die untersuchten Isolate ein Resistenzprofil f\u00fcr nicht-\u00df-Lactam-Antiinfektiva erstellt. Mit den vorgenannten Typisierungsmethoden konnten identische Ergebnisse f\u00fcr alle f\u00fcnf caninen sowie f\u00fcr das eine humane MRSP-Isolat ermittelt werden, sodass alle Isolate dem gleichen Stamm zuzuordnen waren: ein multiresistenter MRSP vom Sequenztyp (ST) 71, SCCmec<\/em>III, spa Typ (t)05), der sich zudem in der PCR positiv f\u00fcr die LukI- und SIETkodierenden Gene zeigte.
Grunds\u00e4tzlich kommen f\u00fcr ein Therapieversagen bzw. f\u00fcr eine Reinfektion zahlreiche Ursachen in Betracht, z. B. der Kontakt zu asymptomatisch besiedelten anderen Hunden oder Menschen, unzureichend desinfizierten oder nur schwer desinfizierbaren Gegenst\u00e4nden oder Fl\u00e4chen im h\u00e4uslichen Bereich sowie die nicht durchgef\u00fchrte Schur des Hundes.
Dennoch deuten diese Ergebnisse auf eine grunds\u00e4tzlich m\u00f6gliche \u00dcbertragbarkeit von MRSP zwischen Hund und Mensch hin und lassen Raum f\u00fcr eine Diskussion \u00fcber die Relevanz von Besitzern als potentielle Reinfektionsquelle f\u00fcr Tiere mit bakteriell bedingten chronischen Hautentz\u00fcndungen. Dar\u00fcber hinaus wurden erst k\u00fcrzlich MRSP-Infektionen beim Menschen beschrieben, jedoch ist das pathogene Potential von multiresistenten MRSP f\u00fcr Menschen bislang unbekannt.","schluesselwoerter":["Methicillin-resistente Staphylococcus pseudintermedius"],"translatedTitle":"","abstractE":"A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round. Comparative typing of all MRSP-isolates by pulsed-field gel electrophoresis (PFGE), SCCmec typing, multilocus sequence typing (MLST), spa typing, PCRdetection of the leukotoxin encoding operon (LukI) and the Staphylococcus intermedius-exfoliative toxin (SIET) as well as antimicrobial resistance profiling by broth microdilution revealed that all five MRSP isolates from the dog and the single isolate from the owner were indistinguishable by any of the applied methods. All isolates were assigned to a certain strain, a multidrug-resistant MRSP belonging to sequence type (ST) 71, spa type (t)05, harbouring SCCmecIII as well as the genes encoding LukI and SIET. In this case, a number of reasons might have contributed to therapy failure and re-infection, respectively (e. g. contact to other MRSP-colonized dogs, contact to MRSP-colonized humans, refusal to clip the dog`s fur). In addition, MRSP-contaminated objects or surfaces in the household, which were difficult to disinfect or simply not considered as a potential source of MRSP, might have served as a source of re-infection. These results envision the possibility of a dog-to-human transmission of MRSP and the relevance of this aspect as a potential source of re-infection in cases of bacterial-supported long-term skin disorders in canine patients. First cases of MRSP infections in humans have been described only recently. However, the general pathogenic potential of multidrug resistant MRSP in humans is unknown so far and needs further investigation.","date":{"year":2010,"date":"08\/2010","accepted":"2010-08-20"},"volume":"123","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"353-358","redirects":["open-access-methicillin-resistant-staphylococcus-pseudintermedius-mrsp-pyoderma-dog-human-transmission\/150\/3216\/70513","open-access-methicillin-resistant-staphylococcus-pseudintermedius-mrsp-pyoderma-dog-human-transmission\/150\/3130\/70513"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 08/2010 DO - 10.2376/0005-9366-123-353 LA - English N2 - A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round.A case of a dog with a long-term inflammatory skin disorder due to infection with methicillin-resistant Staphylococcus pseudintermedius (MRSP) is described. After initial diagnostics of MRSP, follow-up swabs of the dog (nose, skin) were taken twice after four and seven weeks. MRSP was constantly isolated from the skin and once from the nose. Since infected humans might be a source of reinfection, the owners of the dog were screened (nasal) three times during their pet #146;s therapy. Thereby, the male owner was found to be colonized with MRSP once in the first sampling round. Comparative typing of all MRSP-isolates by pulsed-field gel electrophoresis (PFGE), SCCmec typing, multilocus sequence typing (MLST), spa typing, PCRdetection of the leukotoxin encoding operon (LukI) and the Staphylococcus intermedius-exfoliative toxin (SIET) as well as antimicrobial resistance profiling by broth microdilution revealed that all five MRSP isolates from the dog and the single isolate from the owner were indistinguishable by any of the applied methods. All isolates were assigned to a certain strain, a multidrug-resistant MRSP belonging to sequence type (ST) 71, spa type (t)05, harbouring SCCmecIII as well as the genes encoding LukI and SIET. In this case, a number of reasons might have contributed to therapy failure and re-infection, respectively (e. g. contact to other MRSP-colonized dogs, contact to MRSP-colonized humans, refusal to clip the dog`s fur). In addition, MRSP-contaminated objects or surfaces in the household, which were difficult to disinfect or simply not considered as a potential source of MRSP, might have served as a source of re-infection. These results envision the possibility of a dog-to-human transmission of MRSP and the relevance of this aspect as a potential source of re-infection in cases of bacterial-supported long-term skin disorders in canine patients. First cases of MRSP infections in humans have been described only recently. However, the general pathogenic potential of multidrug resistant MRSP in humans is unknown so far and needs further investigation. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2010 SP - 353 EP - 358 T1 - Multidrug- and methicillin resistant Staphylococcus pseudintermedius as a cause of canine pyoderma: a case report T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Multidrug- and methicillin resistant Staphylococcus pseudintermedius as a cause of canine pyoderma: a case report VL - 123 SN - 0005-9366 ER -