TY - JOUR KW - pig KW - heat shock proteins KW - transport KW - stress KW - organ failure AU - E Bao AU - K Sultan AU - B Nowak AU - J Hartung AB - The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps ... BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70394,"title":"Expression of heat shock proteins in tissues from young pigs exposed to transport stress","teaserText":"The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps ...","content":"

Summary<\/span>
The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps expressed in skeletal muscle, heart, liver and kidney of transported youngpigs were investigated. The study comprised 13 German Landrace line pigs (meanweight, approx. 35 \u00b1 1 kg). Eight animals (n = 8) were transported for a period of6 h, whereas the control group (n = 5) was kept under normal housing conditions.Identification of Hsps was performed by immunoblot using porcine specificantibodies. Although the 5 Hsps were regularly detected in all porcine tissues,no clear up-regulation could be observed due to transportation. Densitometricalanalysis of the immunoblots revealed an unexpected result. Every tissue had asignificant reduction of at least 2 Hsp members: Kidney (Hsp90 and Hsp70), M.longissimus dorsi (Hsp90 and Hsp72) and M. gluteus maximus superficialis (Hsp90and Hsp86), liver (Hsp90, Hsp86 and Hsp27). The heart was most affected, all Hspswere significantly reduced by 26% to 41% after 6 h of transportation. The regularreduction of the large Hsp90 and Hsp86 in nearly all tissues examined point toa new and critical role these Hsps might have in counteracting short term stressreactions. The fast reduction of Hsps in fatal organs such as heart and kidney mayhave a relation to organ failure. Hsp90 level may therefore serve as a potentialmarker for the stress pigs are suffering during transportation.

Keywords:<\/span>
pig, heat shock proteins, transport, stress, organ failure


Zusammenfassung<\/span>
Das Ziel der vorliegenden Arbeit war es, die Rolle von Heat Shock Proteinen(Hsps) als m\u00f6gliche Stressmarker bei transportierten Schweinen zu untersuchen.Dazu wurden die konstitutiv (Hsp90, Hsp70 and Hsp27) und induzierbar (Hsp72und Hsp86) exprimierten Hsps im Skelettmuskel, Herzen, Leber und Niere von 13Deutschen Landschweinen (Durchschnittsgewicht ca. 35 \u00b1 1 kg) analysiert. AchtTiere (n = 8) wurden einem 6-h Transport unterzogen, w\u00e4hrend die Kontrolltiere(n = 5) in ihrem gewohnten Stall verblieben. Die Hsps wurden mittels Immunoblotmit schweinespezifischen Antik\u00f6rpern detektiert. Obwohl 5 Hsps regelm\u00e4\u00dfignachgewiesen werden konnten, war eine deutliche Hochregulation aufgrund desTransportes nicht erkennbar. Die densitometrische Analyse der Immunoblots erbrachtejedoch ein unerwartetes Ergebnis. Jedes der untersuchten Organgewebezeigte eine Reduktion von mindestens 2 Hsps: Niere (Hsp90 and Hsp70), M. longissimusdorsi (Hsp90 and Hsp72) und M. gluteus maximus superficialis (Hsp90and Hsp86), Leber (Hsp90, Hsp86 and Hsp27). Diese Hsp-Reduzierung war nachdem 6 h Transport am deutlichsten im Herzen zu verzeichnen, hier waren alleHsps signifikant um 26 % bis 41 % reduziert. Die regelm\u00e4\u00dfig auftretende Reduktionder gro\u00dfen Hsp90 und Hsp86 in fast allen untersuchten Geweben weist aufeine neue und kritische Rolle dieser Hsps bei der m\u00f6glichen Gegenregulationw\u00e4hrend einer Kurzzeit-Stressreaktion hin. Diese rasche Reduktion von Hsps inlebenswichtigen Organen wie Herz und Niere k\u00f6nnte mit dem Versagen dieserOrgane korrelieren. Besonders der Hsp90 Gehalt bietet sich als potentieller Markerf\u00fcr die Transport-induzierte Stresseinwirkung bei Schweinen an.

Schl\u00fcsselw\u00f6rter:<\/span>
Schwein, Hitze-Schock-Proteine, Tiertransport, Stress,Organversagen<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Sep 3, 2009 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/pig-heat-shock-proteins-transport-stress-organ-failure\/150\/3130\/70394"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"M. & H. Schaper GmbH","doiSerialWorkTitle":"Dtsch.tier\u00e4rztl.Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/pig-heat-shock-proteins-transport-stress-organ-failure\/150\/3130\/70394","doiSource":"Dtsch.tier\u00e4rztl.Wschr. 116: 9, 321-325 (2009)","doiissn":"0341-6593","doiNr":"10.2376\/0341-6593-116-321","doiFirstPage":"321","doiLastPage":"325","doiTransmitted":true,"doiAuthor":"Bao E, Sultan KR, Nowak B, Hartung J","pdf":{"path":"http:\/\/data\/dtw_2009_09_0321.pdf","title":"dtw_2009_09_0321.pdf","description":"Expression of heat shock proteins in tissues from young pigs exposed to transport stress

"},"authors":[{"firstName":"E","middleName":"","lastName":"Bao"},{"firstName":"K","middleName":"R","lastName":"Sultan"},{"firstName":"B","middleName":"","lastName":"Nowak"},{"firstName":"J","middleName":"","lastName":"Hartung"}],"contentOptimised":"

Summary<\/strong>
The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps expressed in skeletal muscle, heart, liver and kidney of transported youngpigs were investigated. The study comprised 13 German Landrace line pigs (meanweight, approx. 35 \u00b1 1 kg). Eight animals (n = 8) were transported for a period of6 h, whereas the control group (n = 5) was kept under normal housing conditions.Identification of Hsps was performed by immunoblot using porcine specificantibodies. Although the 5 Hsps were regularly detected in all porcine tissues,no clear up-regulation could be observed due to transportation. Densitometricalanalysis of the immunoblots revealed an unexpected result. Every tissue had asignificant reduction of at least 2 Hsp members: Kidney (Hsp90 and Hsp70), M.longissimus dorsi (Hsp90 and Hsp72) and M. gluteus maximus superficialis (Hsp90and Hsp86), liver (Hsp90, Hsp86 and Hsp27). The heart was most affected, all Hspswere significantly reduced by 26% to 41% after 6 h of transportation. The regularreduction of the large Hsp90 and Hsp86 in nearly all tissues examined point toa new and critical role these Hsps might have in counteracting short term stressreactions. The fast reduction of Hsps in fatal organs such as heart and kidney mayhave a relation to organ failure. Hsp90 level may therefore serve as a potentialmarker for the stress pigs are suffering during transportation.

Keywords:<\/strong>
pig, heat shock proteins, transport, stress, organ failure


Zusammenfassung<\/strong>
Das Ziel der vorliegenden Arbeit war es, die Rolle von Heat Shock Proteinen(Hsps) als m\u00f6gliche Stressmarker bei transportierten Schweinen zu untersuchen.Dazu wurden die konstitutiv (Hsp90, Hsp70 and Hsp27) und induzierbar (Hsp72und Hsp86) exprimierten Hsps im Skelettmuskel, Herzen, Leber und Niere von 13Deutschen Landschweinen (Durchschnittsgewicht ca. 35 \u00b1 1 kg) analysiert. AchtTiere (n = 8) wurden einem 6-h Transport unterzogen, w\u00e4hrend die Kontrolltiere(n = 5) in ihrem gewohnten Stall verblieben. Die Hsps wurden mittels Immunoblotmit schweinespezifischen Antik\u00f6rpern detektiert. Obwohl 5 Hsps regelm\u00e4\u00dfignachgewiesen werden konnten, war eine deutliche Hochregulation aufgrund desTransportes nicht erkennbar. Die densitometrische Analyse der Immunoblots erbrachtejedoch ein unerwartetes Ergebnis. Jedes der untersuchten Organgewebezeigte eine Reduktion von mindestens 2 Hsps: Niere (Hsp90 and Hsp70), M. longissimusdorsi (Hsp90 and Hsp72) und M. gluteus maximus superficialis (Hsp90and Hsp86), Leber (Hsp90, Hsp86 and Hsp27). Diese Hsp-Reduzierung war nachdem 6 h Transport am deutlichsten im Herzen zu verzeichnen, hier waren alleHsps signifikant um 26 % bis 41 % reduziert. Die regelm\u00e4\u00dfig auftretende Reduktionder gro\u00dfen Hsp90 und Hsp86 in fast allen untersuchten Geweben weist aufeine neue und kritische Rolle dieser Hsps bei der m\u00f6glichen Gegenregulationw\u00e4hrend einer Kurzzeit-Stressreaktion hin. Diese rasche Reduktion von Hsps inlebenswichtigen Organen wie Herz und Niere k\u00f6nnte mit dem Versagen dieserOrgane korrelieren. Besonders der Hsp90 Gehalt bietet sich als potentieller Markerf\u00fcr die Transport-induzierte Stresseinwirkung bei Schweinen an.

Schl\u00fcsselw\u00f6rter:<\/strong>
Schwein, Hitze-Schock-Proteine, Tiertransport, Stress,Organversagen<\/p>","primaryLanguage":"englisch","summary":"The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps expressed in skeletal muscle, heart, liver and kidney of transported youngpigs were investigated. The study comprised 13 German Landrace line pigs (meanweight, approx. 35 \u00b1 1 kg). Eight animals (n = 8) were transported for a period of6 h, whereas the control group (n = 5) was kept under normal housing conditions.Identification of Hsps was performed by immunoblot using porcine specificantibodies. Although the 5 Hsps were regularly detected in all porcine tissues,no clear up-regulation could be observed due to transportation. Densitometricalanalysis of the immunoblots revealed an unexpected result. Every tissue had asignificant reduction of at least 2 Hsp members: Kidney (Hsp90 and Hsp70), M.longissimus dorsi (Hsp90 and Hsp72) and M. gluteus maximus superficialis (Hsp90and Hsp86), liver (Hsp90, Hsp86 and Hsp27). The heart was most affected, all Hspswere significantly reduced by 26% to 41% after 6 h of transportation. The regularreduction of the large Hsp90 and Hsp86 in nearly all tissues examined point toa new and critical role these Hsps might have in counteracting short term stressreactions. The fast reduction of Hsps in fatal organs such as heart and kidney mayhave a relation to organ failure. Hsp90 level may therefore serve as a potentialmarker for the stress pigs are suffering during transportation.","keywords":["pig","heat shock proteins","transport","stress","organ failure"],"zusammenfassung":"Das Ziel der vorliegenden Arbeit war es, die Rolle von Heat Shock Proteinen(Hsps) als m\u00f6gliche Stressmarker bei transportierten Schweinen zu untersuchen.Dazu wurden die konstitutiv (Hsp90, Hsp70 and Hsp27) und induzierbar (Hsp72und Hsp86) exprimierten Hsps im Skelettmuskel, Herzen, Leber und Niere von 13Deutschen Landschweinen (Durchschnittsgewicht ca. 35 \u00b1 1 kg) analysiert. AchtTiere (n = 8) wurden einem 6-h Transport unterzogen, w\u00e4hrend die Kontrolltiere(n = 5) in ihrem gewohnten Stall verblieben. Die Hsps wurden mittels Immunoblotmit schweinespezifischen Antik\u00f6rpern detektiert. Obwohl 5 Hsps regelm\u00e4\u00dfignachgewiesen werden konnten, war eine deutliche Hochregulation aufgrund desTransportes nicht erkennbar. Die densitometrische Analyse der Immunoblots erbrachtejedoch ein unerwartetes Ergebnis. Jedes der untersuchten Organgewebezeigte eine Reduktion von mindestens 2 Hsps: Niere (Hsp90 and Hsp70), M. longissimusdorsi (Hsp90 and Hsp72) und M. gluteus maximus superficialis (Hsp90and Hsp86), Leber (Hsp90, Hsp86 and Hsp27). Diese Hsp-Reduzierung war nachdem 6 h Transport am deutlichsten im Herzen zu verzeichnen, hier waren alleHsps signifikant um 26 % bis 41 % reduziert. Die regelm\u00e4\u00dfig auftretende Reduktionder gro\u00dfen Hsp90 und Hsp86 in fast allen untersuchten Geweben weist aufeine neue und kritische Rolle dieser Hsps bei der m\u00f6glichen Gegenregulationw\u00e4hrend einer Kurzzeit-Stressreaktion hin. Diese rasche Reduktion von Hsps inlebenswichtigen Organen wie Herz und Niere k\u00f6nnte mit dem Versagen dieserOrgane korrelieren. Besonders der Hsp90 Gehalt bietet sich als potentieller Markerf\u00fcr die Transport-induzierte Stresseinwirkung bei Schweinen an.","schluesselwoerter":["Schwein","Hitze-Schock-Proteine","Tiertransport","Stress","Organversagen"],"translatedTitle":"","abstractE":"The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps ...","date":{"year":2009,"date":"09\/2009","accepted":"2009-09-03"},"volume":"116","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"321-325","redirects":["pig-heat-shock-proteins-transport-stress-organ-failure\/150\/3130\/70394"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 09/2009 DO - 10.2376/0341-6593-116-321 LA - English N2 - The objective of the present study was to investigate the role of heat shock proteins(Hsps) as potential stress response marker in several organs of transportedpigs. Constitutive (Hsp90, Hsp70 and Hsp27) and inducible (Hsp72 and Hsp86)Hsps ... PB - M. & H. Schaper GmbH PP - Hannover PY - 2009 SP - 321 EP - 325 T1 - Expression of heat shock proteins in tissues from young pigs exposed to transport stress T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Expression of heat shock proteins in tissues from young pigs exposed to transport stress VL - 116 SN - 0341-6593 ER -