TY - JOUR AU - S Barth AU - A Tscholshiew AU - C Menge AU - R Weiss AU - G Baljer AU - R Bauerfeind AB - Fecal Escherichia coli isolates (n = 3,218) from piglets with edema disease or diarrhea were screened forthe genes of Stx2 and Stx2 variants.A total of 283 Ecoli isolates (8.8 %) proved exclusively positive for Stx2e and most of these (85.1 %) harbored genes for F18 fimbria.No recognized adhesins were detectable in 14.5 % of the isolates.Genes for heat-stableor heat-labile E.coli enterotoxins were found in F18+ as well as F18" isolates (51.9 %and 33.3 %, respectively). Five isolates also harbored fyuA and irp2 genes which are indicative of a high pathogenicity island in E.coli. All Stx2e+ isolates lacked genes for intimin, EHEC hemolysin,STEC autoag-glutinating adhesin, subtilase cytotoxin,serine protease Espl.The majority of Stx2e+ isolates belonged to phylogenetic groups A (59.3 %) and D (38.9 %) and only few isolates were classified as B1 and B2 (1.8 %).The results suggest that Stx2e-produ-cing E coli strains are highly prevalent in diseased pigs in Germany. Despite their significant diversity, most strains possess all typical features (Stx2e, F18) of porcine edema disease E.coli. However, a considerable portion of porcine strains resembles published human Stx2e+ strains in that they lackany recognized pig-associated adhesin.Thus,a zoonotic potential cannot be excluded for these strains. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70369,"title":"Virulence and fitness gene patterns of Shiga toxin-encoding Escherichio coli isolated from pigs with edema disease or diarrhea in Germany","teaserText":"DOI 10.2376\/0005-9366-120-307","content":"

Summary<\/span>
Fecal Escherichia coli isolates (n = 3,218) from piglets with edema disease or diarrhea were screened forthe genes of Stx2 and Stx2 variants.A total of 283 Ecoli isolates (8.8 %) proved exclusively positive for Stx2e and most of these (85.1 %) harbored genes for F18 fimbria.No recognized adhesins were detectable in 14.5 % of the isolates.Genes for heat-stableor heat-labile E.coli enterotoxins were found in F18+ as well as F18\" isolates (51.9 %and 33.3 %, respectively). Five isolates also harbored fyuA and irp2 genes which are indicative of a high pathogenicity island in E.coli. All Stx2e+ isolates lacked genes for intimin, EHEC hemolysin,STEC autoag-glutinating adhesin, subtilase cytotoxin,serine protease Espl.The majority of Stx2e+ isolates belonged to phylogenetic groups A (59.3 %) and D (38.9 %) and only few isolates were classified as B1 and B2 (1.8 %).The results suggest that Stx2e-produ-cing E coli strains are highly prevalent in diseased pigs in Germany. Despite their significant diversity, most strains possess all typical features (Stx2e, F18) of porcine edema disease E.coli. However, a considerable portion of porcine strains resembles published human Stx2e+ strains in that they lackany recognized pig-associated adhesin.Thus,a zoonotic potential cannot be excluded for these strains.


Zusammenfassung<\/span>
Escherichia co\/\/-lsolate (n = 3.218) von erkrankten Ferkeln und Mastschweinen wurden auf die Shigatoxin-Gene stx2 und sfx2-Varianten untersucht. Es reagierten 283 Isolate (8,8 %) positiv, wobei alle positiven Isolate ausschlie\u00dflich den Subtyp Stx2e kodierten. Die meisten Isolate besa\u00dfen auch Gene f\u00fcr die schweinespezifischen, adh\u00e4siven F18-Fimbrien (85,1 %). Bei 14,5 % der Isolate lie\u00dfen sich keinerlei bekannte Adh\u00e4sionsfaktoren nachweisen.Gene f\u00fcr hitzestabile und -labile E.coli-Enterotoxine waren sowohl bei F18+- als auch bei F18\"-lsolaten nachweisbar (51,9 % bzw. 33,3 %). F\u00fcnf Isolate besa\u00dfen auch die beiden im\u201ehigh pathogenicity island\" von E.coli lokalisierten Gene fyuA und irp2. Die Gene f\u00fcr Intimin,das EHEC-H\u00e4molysin,das\u201eSTEC autoagglutinating adhesin\", das Subtilase-Zytotoxin und die Serinprotease Espl waren bei keinem Isolat nachweisbar. Die meisten porzinen Stx2e+ Isolate geh\u00f6rten den phylogenetischen Gruppen A und D (59,3 % bzw. 38,9 %) an und nur wenige den Gruppen B1 und B2 (zusammen 1,8 %). Demnach scheiden erkrankte Ferkel und Mastschweine in Deutschland h\u00e4ufig Stx2e-kodie-rende E.coli aus. Diese Isolate besitzen trotz ihrer gro\u00dfen Heterogenit\u00e4t in der Mehrzahl alle typischen Merkmale der \u00d6demkrankheit-f. coli (Stx2e, F18). Einer kleineren Anzahl an St\u00e4mmen fehlen aber bekannte schweinespezifische Adh\u00e4si-ne, weshalb sie den von Menschen isolierten Stx2e-kodierenden St\u00e4mmen \u00e4hneln. Es kann gegenw\u00e4rtig nicht ausgeschlossen werden, dass es sich bei diesen St\u00e4mmen um Zoonoseerreger handelt.<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jul 1, 2007 12:00:00 AM","toDate":"Jan 1, 2030 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/16737413\/150\/3130\/70369"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/16737413\/150\/3130\/70369","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 120: 7-8, 307-316 (2007)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-120-307","doiFirstPage":"307","doiLastPage":"316","doiTransmitted":true,"doiAuthor":"Barth S, Tscholshiew A, Menge C, Wei\u00df R, Baljer G, Bauerfeind R","pdf":{"path":"http:\/\/data\/bmtw_2007_07_0307.pdf","title":"bmtw_2007_07_0307.pdf","description":"Virulence and fitness gene patterns of Shiga toxin-encoding Escherichio coli isolated from pigs with edema disease or diarrhea in Germany

"},"authors":[{"firstName":"S","middleName":"","lastName":"Barth"},{"firstName":"A","middleName":"","lastName":"Tscholshiew"},{"firstName":"C","middleName":"","lastName":"Menge"},{"firstName":"R","middleName":"","lastName":"Wei\u00df"},{"firstName":"G","middleName":"","lastName":"Baljer"},{"firstName":"R","middleName":"","lastName":"Bauerfeind"}],"contentOptimised":"

Summary<\/strong>
Fecal Escherichia coli isolates (n = 3,218) from piglets with edema disease or diarrhea were screened forthe genes of Stx2 and Stx2 variants.A total of 283 Ecoli isolates (8.8 %) proved exclusively positive for Stx2e and most of these (85.1 %) harbored genes for F18 fimbria.No recognized adhesins were detectable in 14.5 % of the isolates.Genes for heat-stableor heat-labile E.coli enterotoxins were found in F18+ as well as F18\" isolates (51.9 %and 33.3 %, respectively). Five isolates also harbored fyuA and irp2 genes which are indicative of a high pathogenicity island in E.coli. All Stx2e+ isolates lacked genes for intimin, EHEC hemolysin,STEC autoag-glutinating adhesin, subtilase cytotoxin,serine protease Espl.The majority of Stx2e+ isolates belonged to phylogenetic groups A (59.3 %) and D (38.9 %) and only few isolates were classified as B1 and B2 (1.8 %).The results suggest that Stx2e-produ-cing E coli strains are highly prevalent in diseased pigs in Germany. Despite their significant diversity, most strains possess all typical features (Stx2e, F18) of porcine edema disease E.coli. However, a considerable portion of porcine strains resembles published human Stx2e+ strains in that they lackany recognized pig-associated adhesin.Thus,a zoonotic potential cannot be excluded for these strains.


Zusammenfassung<\/strong>
Escherichia co\/\/-lsolate (n = 3.218) von erkrankten Ferkeln und Mastschweinen wurden auf die Shigatoxin-Gene stx2 und sfx2-Varianten untersucht. Es reagierten 283 Isolate (8,8 %) positiv, wobei alle positiven Isolate ausschlie\u00dflich den Subtyp Stx2e kodierten. Die meisten Isolate besa\u00dfen auch Gene f\u00fcr die schweinespezifischen, adh\u00e4siven F18-Fimbrien (85,1 %). Bei 14,5 % der Isolate lie\u00dfen sich keinerlei bekannte Adh\u00e4sionsfaktoren nachweisen.Gene f\u00fcr hitzestabile und -labile E.coli-Enterotoxine waren sowohl bei F18+- als auch bei F18\"-lsolaten nachweisbar (51,9 % bzw. 33,3 %). F\u00fcnf Isolate besa\u00dfen auch die beiden im\u201ehigh pathogenicity island\" von E.coli lokalisierten Gene fyuA und irp2. Die Gene f\u00fcr Intimin,das EHEC-H\u00e4molysin,das\u201eSTEC autoagglutinating adhesin\", das Subtilase-Zytotoxin und die Serinprotease Espl waren bei keinem Isolat nachweisbar. Die meisten porzinen Stx2e+ Isolate geh\u00f6rten den phylogenetischen Gruppen A und D (59,3 % bzw. 38,9 %) an und nur wenige den Gruppen B1 und B2 (zusammen 1,8 %). Demnach scheiden erkrankte Ferkel und Mastschweine in Deutschland h\u00e4ufig Stx2e-kodie-rende E.coli aus. Diese Isolate besitzen trotz ihrer gro\u00dfen Heterogenit\u00e4t in der Mehrzahl alle typischen Merkmale der \u00d6demkrankheit-f. coli (Stx2e, F18). Einer kleineren Anzahl an St\u00e4mmen fehlen aber bekannte schweinespezifische Adh\u00e4si-ne, weshalb sie den von Menschen isolierten Stx2e-kodierenden St\u00e4mmen \u00e4hneln. Es kann gegenw\u00e4rtig nicht ausgeschlossen werden, dass es sich bei diesen St\u00e4mmen um Zoonoseerreger handelt.<\/p>","primaryLanguage":"englisch","summary":"Fecal Escherichia coli isolates (n = 3,218) from piglets with edema disease or diarrhea were screened forthe genes of Stx2 and Stx2 variants.A total of 283 Ecoli isolates (8.8 %) proved exclusively positive for Stx2e and most of these (85.1 %) harbored genes for F18 fimbria.No recognized adhesins were detectable in 14.5 % of the isolates.Genes for heat-stableor heat-labile E.coli enterotoxins were found in F18+ as well as F18\" isolates (51.9 %and 33.3 %, respectively). Five isolates also harbored fyuA and irp2 genes which are indicative of a high pathogenicity island in E.coli. All Stx2e+ isolates lacked genes for intimin, EHEC hemolysin,STEC autoag-glutinating adhesin, subtilase cytotoxin,serine protease Espl.The majority of Stx2e+ isolates belonged to phylogenetic groups A (59.3 %) and D (38.9 %) and only few isolates were classified as B1 and B2 (1.8 %).The results suggest that Stx2e-produ-cing E coli strains are highly prevalent in diseased pigs in Germany. Despite their significant diversity, most strains possess all typical features (Stx2e, F18) of porcine edema disease E.coli. However, a considerable portion of porcine strains resembles published human Stx2e+ strains in that they lackany recognized pig-associated adhesin.Thus,a zoonotic potential cannot be excluded for these strains.","zusammenfassung":"Escherichia co\/\/-lsolate (n = 3.218) von erkrankten Ferkeln und Mastschweinen wurden auf die Shigatoxin-Gene stx2 und sfx2-Varianten untersucht. Es reagierten 283 Isolate (8,8 %) positiv, wobei alle positiven Isolate ausschlie\u00dflich den Subtyp Stx2e kodierten. Die meisten Isolate besa\u00dfen auch Gene f\u00fcr die schweinespezifischen, adh\u00e4siven F18-Fimbrien (85,1 %). Bei 14,5 % der Isolate lie\u00dfen sich keinerlei bekannte Adh\u00e4sionsfaktoren nachweisen.Gene f\u00fcr hitzestabile und -labile E.coli-Enterotoxine waren sowohl bei F18+- als auch bei F18\"-lsolaten nachweisbar (51,9 % bzw. 33,3 %). F\u00fcnf Isolate besa\u00dfen auch die beiden im\u201ehigh pathogenicity island\" von E.coli lokalisierten Gene fyuA und irp2. Die Gene f\u00fcr Intimin,das EHEC-H\u00e4molysin,das\u201eSTEC autoagglutinating adhesin\", das Subtilase-Zytotoxin und die Serinprotease Espl waren bei keinem Isolat nachweisbar. Die meisten porzinen Stx2e+ Isolate geh\u00f6rten den phylogenetischen Gruppen A und D (59,3 % bzw. 38,9 %) an und nur wenige den Gruppen B1 und B2 (zusammen 1,8 %). Demnach scheiden erkrankte Ferkel und Mastschweine in Deutschland h\u00e4ufig Stx2e-kodie-rende E.coli aus. Diese Isolate besitzen trotz ihrer gro\u00dfen Heterogenit\u00e4t in der Mehrzahl alle typischen Merkmale der \u00d6demkrankheit-f. coli (Stx2e, F18). Einer kleineren Anzahl an St\u00e4mmen fehlen aber bekannte schweinespezifische Adh\u00e4si-ne, weshalb sie den von Menschen isolierten Stx2e-kodierenden St\u00e4mmen \u00e4hneln. Es kann gegenw\u00e4rtig nicht ausgeschlossen werden, dass es sich bei diesen St\u00e4mmen um Zoonoseerreger handelt.<\/p>","translatedTitle":"DOI 10.2376\/0005-9366-120-307","abstractE":"Fecal Escherichia coli isolates (n = 3,218) from piglets with edema disease or diarrhea were screened forthe genes of Stx2 and Stx2 variants.A total of 283 Ecoli isolates (8.8 %) proved exclusively positive for Stx2e and most of these (85.1 %) harbored genes for F18 fimbria.No recognized adhesins were detectable in 14.5 % of the isolates.Genes for heat-stableor heat-labile E.coli enterotoxins were found in F18+ as well as F18\" isolates (51.9 %and 33.3 %, respectively). Five isolates also harbored fyuA and irp2 genes which are indicative of a high pathogenicity island in E.coli. All Stx2e+ isolates lacked genes for intimin, EHEC hemolysin,STEC autoag-glutinating adhesin, subtilase cytotoxin,serine protease Espl.The majority of Stx2e+ isolates belonged to phylogenetic groups A (59.3 %) and D (38.9 %) and only few isolates were classified as B1 and B2 (1.8 %).The results suggest that Stx2e-produ-cing E coli strains are highly prevalent in diseased pigs in Germany. Despite their significant diversity, most strains possess all typical features (Stx2e, F18) of porcine edema disease E.coli. However, a considerable portion of porcine strains resembles published human Stx2e+ strains in that they lackany recognized pig-associated adhesin.Thus,a zoonotic potential cannot be excluded for these strains.","date":{"year":2007,"date":"07\/2007","accepted":"2007-07-01"},"volume":"120","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"307-316","redirects":["16737413\/150\/3130\/70369"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 07/2007 DO - 10.2376/0005-9366-120-307 LA - English N2 - Fecal Escherichia coli isolates (n = 3,218) from piglets with edema disease or diarrhea were screened forthe genes of Stx2 and Stx2 variants.A total of 283 Ecoli isolates (8.8 %) proved exclusively positive for Stx2e and most of these (85.1 %) harbored genes for F18 fimbria.No recognized adhesins were detectable in 14.5 % of the isolates.Genes for heat-stableor heat-labile E.coli enterotoxins were found in F18+ as well as F18" isolates (51.9 %and 33.3 %, respectively). Five isolates also harbored fyuA and irp2 genes which are indicative of a high pathogenicity island in E.coli. All Stx2e+ isolates lacked genes for intimin, EHEC hemolysin,STEC autoag-glutinating adhesin, subtilase cytotoxin,serine protease Espl.The majority of Stx2e+ isolates belonged to phylogenetic groups A (59.3 %) and D (38.9 %) and only few isolates were classified as B1 and B2 (1.8 %).The results suggest that Stx2e-produ-cing E coli strains are highly prevalent in diseased pigs in Germany. Despite their significant diversity, most strains possess all typical features (Stx2e, F18) of porcine edema disease E.coli. However, a considerable portion of porcine strains resembles published human Stx2e+ strains in that they lackany recognized pig-associated adhesin.Thus,a zoonotic potential cannot be excluded for these strains. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2007 SP - 307 EP - 316 T1 - Virulence and fitness gene patterns of Shiga toxin-encoding Escherichio coli isolated from pigs with edema disease or diarrhea in Germany T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Virulence and fitness gene patterns of Shiga toxin-encoding Escherichio coli isolated from pigs with edema disease or diarrhea in Germany TT - DOI 10.2376/0005-9366-120-307 VL - 120 SN - 0005-9366 ER -