TY - JOUR KW - Saccharomycetales KW - psittacines KW - passerines KW - avian gastric yeast AU - R Püstow AU - K Cramer AU - M-E Krautwald-Junghanns AU - V Schmidt AB - Macrorhabdus (M.) ornithogaster is a common cause of fungal gastric disease in passerines and psittacines held in captivity as well as in free-range birds with a worldwide distribution. However, despite culturing of M. ornithogaster is standardized since nearly 10 years now, only three nucleotide sequences isolated from two bird species are currently available in GenBank (http://www.ncbi.nlm. nih.gov) referencing ribosomal DNA fragments. Therefore, the aim of this study was to gain knowledge on the occurrence of different genotypes within specific bird species as well as to optimize molecular biological characterization of this common avian pathogenic yeast. Isolates (n = 15) were obtained from nine individuals belonging to five bird species. Samples of the proventricular isthmus as well as isolates gained after culturing of content from proventricular isthmus have been amplified and sequenced. Successful amplification and sequencing of M. ornithogaster-specific small subunit ribosomal DNA (SSU), ITS-1 gene and the first part of the large ribosomal DNA (D1/D2) was possible in almost all isolates and samples. However, use of cultured isolates is preferable in order to avoid or at least to identify contamination. According to the present gene sequence analysis, M. ornithogaster represents genomic variability inside the ribosomal genome. Further phylogenetic studies are needed to clarify the epidemiological impact of this variability. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":102968,"title":"Ribosomal DNA fragments analysis of avian pathogenic Macrorhabdus ornithogaster","topline":"","teaserText":"Analyse von ribosomalen DNA-Fragmenten von vogelpathogenen Macrorhabdus ornithogaster","content":"

Summary<\/span>
Macrorhabdus (M.) ornithogaster is a common cause of fungal gastric disease in passerines and psittacines held in captivity as well as in free-range birds with a worldwide distribution. However, despite culturing of M. ornithogaster is standardized since nearly 10 years now, only three nucleotide sequences isolated from two bird species are currently available in GenBank (http:\/\/www.ncbi.nlm. nih.gov) referencing ribosomal DNA fragments. Therefore, the aim of this study was to gain knowledge on the occurrence of different genotypes within specific bird species as well as to optimize molecular biological characterization of this common avian pathogenic yeast. Isolates (n = 15) were obtained from nine individuals belonging to five bird species. Samples of the proventricular isthmus as well as isolates gained after culturing of content from proventricular isthmus have been amplified and sequenced. Successful amplification and sequencing of M. ornithogaster-specific small subunit ribosomal DNA (SSU), ITS-1 gene and the first part of the large ribosomal DNA (D1\/D2) was possible in almost all isolates and samples. However, use of cultured isolates is preferable in order to avoid or at least to identify contamination. According to the present gene sequence analysis, M. ornithogaster represents genomic variability inside the ribosomal genome. Further phylogenetic studies are needed to clarify the epidemiological impact of this variability. <\/p>

Keywords<\/span>
Saccharomycetales, psittacines, passerines, avian gastric yeast<\/p>

Zusammenfassung<\/span>
Macrorhabdus (M.) ornithogaster ist eine h\u00e4ufige Ursache von Mykosen des Dr\u00fcsenmagens bei Sperlingsv\u00f6geln und Papageienv\u00f6geln in Gefangenschaft sowie bei freilebenden V\u00f6geln mit einem weltweitem Vorkommen. Obwohl die Kultivierung von M. ornithogaster seit fast 10 Jahren standardisiert ist, sind nur drei Nukleotid-Sequenzen ribosomaler DNA-Fragmente, welche von zwei Vogelarten isoliert wurden, derzeit in GenBank (http:\/\/www.ncbi.nlm.nih.gov) als Referenzen vorhanden. Daher war das Ziel dieser Studie Kenntnis \u00fcber das Auftreten verschiedener Genotypen innerhalb bestimmter Vogelarten zu gewinnen, sowie die molekularbiologische Charakterisierung dieses vogelpathogenen Hefepilzes zu optimieren. Die Isolate (n = 15) wurden aus neun V\u00f6geln, welche zu f\u00fcnf Vogelarten geh\u00f6rten, isoliert. Proben aus dem Isthmus des Dr\u00fcsenmagens sowie Isolate, welche nach Kultivierung des Inhaltes vom Dr\u00fcsenmagenisthmus gewonnen wurden, wurden amplifiziert und sequenziert. Erfolgreiche Amplifikation und Sequenzierung von M. ornithogaster spezifischer small subunit ribosomal Dann (SSU), des ITS-1-Gens und dem erstem Teil der gro\u00dfen ribosomalen DNA (D1\/ D2) konnte in nahezu allen Isolaten und Proben erreicht werden. Die Verwendung von kultivierten Isolaten ist jedoch zu bevorzugen, um Kontaminationen zu verhindern bzw. diese zu identifizieren. Nach der Analyse der vorliegenden Sequenzen, ist festzustellen, dass M. ornithogaster eine genetische Variabilit\u00e4t im ribosomalem Genom aufweist. Weitere phylogenetische Studien sind notwendig, um die epidemiologische Bedeutung dieser Variabilit\u00e4t zu kl\u00e4ren. <\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Saccharomycetales, Psittaciformes, Passeriformes, Avian Gastric Yeast<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jun 21, 2017 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/ribosomal-dna-fragments-analysis-of-avian-pathogenic-macrorhabdus-ornithogaster\/150\/3130\/102968"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/ribosomal-dna-fragments-analysis-of-avian-pathogenic-macrorhabdus-ornithogaster\/150\/3130\/102968\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 2017, aop","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16081","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"P\u00fcstow R, Cramer K, Krautwald-Junghanns M, Schmidt V","pdf":{"path":"http:\/\/data\/BUM_AOP_16081.pdf","title":"BUM_AOP_16081.pdf","description":"Ribosomal DNA fragments analysis of avian pathogenic Macrorhabdus ornithogaster"},"authors":[{"firstName":"R","middleName":"","lastName":"P\u00fcstow"},{"firstName":"K","middleName":"","lastName":"Cramer"},{"firstName":"M","middleName":"","lastName":"Krautwald-Junghanns"},{"firstName":"V","middleName":"","lastName":"Schmidt"}],"contentOptimised":"

Summary<\/strong>
Macrorhabdus (M.) ornithogaster is a common cause of fungal gastric disease in passerines and psittacines held in captivity as well as in free-range birds with a worldwide distribution. However, despite culturing of M. ornithogaster is standardized since nearly 10 years now, only three nucleotide sequences isolated from two bird species are currently available in GenBank (http:\/\/www.ncbi.nlm. nih.gov) referencing ribosomal DNA fragments. Therefore, the aim of this study was to gain knowledge on the occurrence of different genotypes within specific bird species as well as to optimize molecular biological characterization of this common avian pathogenic yeast. Isolates (n = 15) were obtained from nine individuals belonging to five bird species. Samples of the proventricular isthmus as well as isolates gained after culturing of content from proventricular isthmus have been amplified and sequenced. Successful amplification and sequencing of M. ornithogaster-specific small subunit ribosomal DNA (SSU), ITS-1 gene and the first part of the large ribosomal DNA (D1\/D2) was possible in almost all isolates and samples. However, use of cultured isolates is preferable in order to avoid or at least to identify contamination. According to the present gene sequence analysis, M. ornithogaster represents genomic variability inside the ribosomal genome. Further phylogenetic studies are needed to clarify the epidemiological impact of this variability. <\/p>

Keywords:<\/strong>
Saccharomycetales, psittacines, passerines, avian gastric yeast<\/p>

Zusammenfassung<\/strong>
Macrorhabdus (M.) ornithogaster ist eine h\u00e4ufige Ursache von Mykosen des Dr\u00fcsenmagens bei Sperlingsv\u00f6geln und Papageienv\u00f6geln in Gefangenschaft sowie bei freilebenden V\u00f6geln mit einem weltweitem Vorkommen. Obwohl die Kultivierung von M. ornithogaster seit fast 10 Jahren standardisiert ist, sind nur drei Nukleotid-Sequenzen ribosomaler DNA-Fragmente, welche von zwei Vogelarten isoliert wurden, derzeit in GenBank (http:\/\/www.ncbi.nlm.nih.gov) als Referenzen vorhanden. Daher war das Ziel dieser Studie Kenntnis \u00fcber das Auftreten verschiedener Genotypen innerhalb bestimmter Vogelarten zu gewinnen, sowie die molekularbiologische Charakterisierung dieses vogelpathogenen Hefepilzes zu optimieren. Die Isolate (n = 15) wurden aus neun V\u00f6geln, welche zu f\u00fcnf Vogelarten geh\u00f6rten, isoliert. Proben aus dem Isthmus des Dr\u00fcsenmagens sowie Isolate, welche nach Kultivierung des Inhaltes vom Dr\u00fcsenmagenisthmus gewonnen wurden, wurden amplifiziert und sequenziert. Erfolgreiche Amplifikation und Sequenzierung von M. ornithogaster spezifischer small subunit ribosomal Dann (SSU), des ITS-1-Gens und dem erstem Teil der gro\u00dfen ribosomalen DNA (D1\/ D2) konnte in nahezu allen Isolaten und Proben erreicht werden. Die Verwendung von kultivierten Isolaten ist jedoch zu bevorzugen, um Kontaminationen zu verhindern bzw. diese zu identifizieren. Nach der Analyse der vorliegenden Sequenzen, ist festzustellen, dass M. ornithogaster eine genetische Variabilit\u00e4t im ribosomalem Genom aufweist. Weitere phylogenetische Studien sind notwendig, um die epidemiologische Bedeutung dieser Variabilit\u00e4t zu kl\u00e4ren. <\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Saccharomycetales, Psittaciformes, Passeriformes, Avian Gastric Yeast<\/p>","primaryLanguage":"englisch","summary":"Macrorhabdus (M.) ornithogaster is a common cause of fungal gastric disease in passerines and psittacines held in captivity as well as in free-range birds with a worldwide distribution. However, despite culturing of M. ornithogaster is standardized since nearly 10 years now, only three nucleotide sequences isolated from two bird species are currently available in GenBank (http:\/\/www.ncbi.nlm. nih.gov) referencing ribosomal DNA fragments. Therefore, the aim of this study was to gain knowledge on the occurrence of different genotypes within specific bird species as well as to optimize molecular biological characterization of this common avian pathogenic yeast. Isolates (n = 15) were obtained from nine individuals belonging to five bird species. Samples of the proventricular isthmus as well as isolates gained after culturing of content from proventricular isthmus have been amplified and sequenced. Successful amplification and sequencing of M. ornithogaster-specific small subunit ribosomal DNA (SSU), ITS-1 gene and the first part of the large ribosomal DNA (D1\/D2) was possible in almost all isolates and samples. However, use of cultured isolates is preferable in order to avoid or at least to identify contamination. According to the present gene sequence analysis, M. ornithogaster represents genomic variability inside the ribosomal genome. Further phylogenetic studies are needed to clarify the epidemiological impact of this variability. <\/p>

","keywords":["Saccharomycetales","psittacines","passerines","avian gastric yeast"],"zusammenfassung":"Macrorhabdus (M.) ornithogaster ist eine h\u00e4ufige Ursache von Mykosen des Dr\u00fcsenmagens bei Sperlingsv\u00f6geln und Papageienv\u00f6geln in Gefangenschaft sowie bei freilebenden V\u00f6geln mit einem weltweitem Vorkommen. Obwohl die Kultivierung von M. ornithogaster seit fast 10 Jahren standardisiert ist, sind nur drei Nukleotid-Sequenzen ribosomaler DNA-Fragmente, welche von zwei Vogelarten isoliert wurden, derzeit in GenBank (http:\/\/www.ncbi.nlm.nih.gov) als Referenzen vorhanden. Daher war das Ziel dieser Studie Kenntnis \u00fcber das Auftreten verschiedener Genotypen innerhalb bestimmter Vogelarten zu gewinnen, sowie die molekularbiologische Charakterisierung dieses vogelpathogenen Hefepilzes zu optimieren. Die Isolate (n = 15) wurden aus neun V\u00f6geln, welche zu f\u00fcnf Vogelarten geh\u00f6rten, isoliert. Proben aus dem Isthmus des Dr\u00fcsenmagens sowie Isolate, welche nach Kultivierung des Inhaltes vom Dr\u00fcsenmagenisthmus gewonnen wurden, wurden amplifiziert und sequenziert. Erfolgreiche Amplifikation und Sequenzierung von M. ornithogaster spezifischer small subunit ribosomal Dann (SSU), des ITS-1-Gens und dem erstem Teil der gro\u00dfen ribosomalen DNA (D1\/ D2) konnte in nahezu allen Isolaten und Proben erreicht werden. Die Verwendung von kultivierten Isolaten ist jedoch zu bevorzugen, um Kontaminationen zu verhindern bzw. diese zu identifizieren. Nach der Analyse der vorliegenden Sequenzen, ist festzustellen, dass M. ornithogaster eine genetische Variabilit\u00e4t im ribosomalem Genom aufweist. Weitere phylogenetische Studien sind notwendig, um die epidemiologische Bedeutung dieser Variabilit\u00e4t zu kl\u00e4ren. <\/p>

","schluesselwoerter":["Saccharomycetales","Psittaciformes","Passeriformes","Avian Gastric Yeast"],"translatedTitle":"Analyse von ribosomalen DNA-Fragmenten von vogelpathogenen Macrorhabdus ornithogaster","abstractE":"Macrorhabdus (M.) ornithogaster is a common cause of fungal gastric disease in passerines and psittacines held in captivity as well as in free-range birds with a worldwide distribution. However, despite culturing of M. ornithogaster is standardized since nearly 10 years now, only three nucleotide sequences isolated from two bird species are currently available in GenBank (http:\/\/www.ncbi.nlm. nih.gov) referencing ribosomal DNA fragments. Therefore, the aim of this study was to gain knowledge on the occurrence of different genotypes within specific bird species as well as to optimize molecular biological characterization of this common avian pathogenic yeast. Isolates (n = 15) were obtained from nine individuals belonging to five bird species. Samples of the proventricular isthmus as well as isolates gained after culturing of content from proventricular isthmus have been amplified and sequenced. Successful amplification and sequencing of M. ornithogaster-specific small subunit ribosomal DNA (SSU), ITS-1 gene and the first part of the large ribosomal DNA (D1\/D2) was possible in almost all isolates and samples. However, use of cultured isolates is preferable in order to avoid or at least to identify contamination. According to the present gene sequence analysis, M. ornithogaster represents genomic variability inside the ribosomal genome. Further phylogenetic studies are needed to clarify the epidemiological impact of this variability. ","date":{"year":2017,"date":"06\/2017","accepted":"2017-06-21"},"volume":"2017","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["ribosomal-dna-fragments-analysis-of-avian-pathogenic-macrorhabdus-ornithogaster\/150\/3130\/102968"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 06/2017 DO - 10.2376/0005-9366-16081 LA - English N2 - Macrorhabdus (M.) ornithogaster is a common cause of fungal gastric disease in passerines and psittacines held in captivity as well as in free-range birds with a worldwide distribution. However, despite culturing of M. ornithogaster is standardized since nearly 10 years now, only three nucleotide sequences isolated from two bird species are currently available in GenBank (http://www.ncbi.nlm. nih.gov) referencing ribosomal DNA fragments. Therefore, the aim of this study was to gain knowledge on the occurrence of different genotypes within specific bird species as well as to optimize molecular biological characterization of this common avian pathogenic yeast. Isolates (n = 15) were obtained from nine individuals belonging to five bird species. Samples of the proventricular isthmus as well as isolates gained after culturing of content from proventricular isthmus have been amplified and sequenced. Successful amplification and sequencing of M. ornithogaster-specific small subunit ribosomal DNA (SSU), ITS-1 gene and the first part of the large ribosomal DNA (D1/D2) was possible in almost all isolates and samples. However, use of cultured isolates is preferable in order to avoid or at least to identify contamination. According to the present gene sequence analysis, M. ornithogaster represents genomic variability inside the ribosomal genome. Further phylogenetic studies are needed to clarify the epidemiological impact of this variability. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 T1 - Ribosomal DNA fragments analysis of avian pathogenic Macrorhabdus ornithogaster T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Ribosomal DNA fragments analysis of avian pathogenic Macrorhabdus ornithogaster TT - Analyse von ribosomalen DNA-Fragmenten von vogelpathogenen Macrorhabdus ornithogaster VL - 130 SN - 0005-9366 ER -