TY - JOUR KW - mammals KW - faecal samples KW - steroids KW - cortisol metabolites KW - enzyme immunoassay AU - M Malikova AU - E Voslarova AU - V Vecerek AB - Non-invasive monitoring of faecal metabolites of glucocorticoid hormones over the last two decades has become a frequently used method of assessing stress responses in wild animals. The number of studies focusing on this area of research is constantly growing, and also growing is the number of animals for which these methods have been described and validated in a variety of potentially stressful situations. The following text summarizes the advantages of non-invasive methods, particularly analysis of faecal samples, over invasive methods and their application in assessing stress in wild animals. Reference to species in which these methods have been successfully applied is given. Metabolism and excretion of glucocorticoid hormones and their elimination in the form of metabolites in faeces are described. Finally, the paper summarizes the principles of proper sample collection and their storage, and also discusses possible methods of analysis of those samples, which consists of extraction and immunoassay. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":102969,"title":"Non-invasive faecal monitoring of stress in captive and free-ranging wild animals","topline":"","teaserText":"Nichtinvasives Stressmonitoring anhand von Kotproben bei Wildtieren in Zoos und im Freiland","content":"

Summary<\/span>
Non-invasive monitoring of faecal metabolites of glucocorticoid hormones over the last two decades has become a frequently used method of assessing stress responses in wild animals. The number of studies focusing on this area of research is constantly growing, and also growing is the number of animals for which these methods have been described and validated in a variety of potentially stressful situations. The following text summarizes the advantages of non-invasive methods, particularly analysis of faecal samples, over invasive methods and their application in assessing stress in wild animals. Reference to species in which these methods have been successfully applied is given. Metabolism and excretion of glucocorticoid hormones and their elimination in the form of metabolites in faeces are described. Finally, the paper summarizes the principles of proper sample collection and their storage, and also discusses possible methods of analysis of those samples, which consists of extraction and immunoassay.<\/p>

Keywords<\/span>
mammals, faecal samples, steroids, cortisol metabolites, enzyme immunoassay<\/p>

Zusammenfassung<\/span>
Das nichtinvasive Monitoring von Glucocorticoid-Metaboliten im Kot frei lebender Tiere wird in den zwei letzten Dekaden immer h\u00e4ufiger zum Beurteilen der Reaktion der Tiere auf Stress eingesetzt. Dieser Forschungsbereich ist deshalb im Wachstum begriffen, und auch die Anzahl an Tierarten nimmt zu, f\u00fcr die diese Methoden beschrieben und validiert wurden. Im nachfolgenden Text werden die Vorteile nichtinvasiver Methoden, insbesondere die Analyse von Kotproben, im Vergleich zu invasiven zusammengefasst sowie deren Anwendung beim Beurteilen von Stress bei frei lebenden Tieren erl\u00e4utert. Der Verweis auf Tierarten, bei denen diese Methoden erfolgreich angewandt wurden, wird angef\u00fchrt. In weiteren Passagen werden Metabolismus und Exkretion von Glucocorticoiden sowie deren Ausscheidung in Form von Metaboliten in den Kot beschrieben. Ferner werden die Grunds\u00e4tze f\u00fcr richtiges Sammeln und Lagern der Proben zusammengefasst und m\u00f6gliche Methoden f\u00fcr deren Analyse beschrieben, bestehend aus Extraktion und eigentlicher Immunanalyse.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
S\u00e4ugetiere, Kotproben, Steroidhormone, Kortisolmetaboliten, Enzymimmunoassay<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jun 21, 2017 10:00:00 PM","oldUrls":["http:\/\/vetline.de\/non-invasive-faecal-monitoring-of-stress-in-captive-and-free-ranging-wild-animals\/150\/3130\/102969"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/non-invasive-faecal-monitoring-of-stress-in-captive-and-free-ranging-wild-animals\/150\/3130\/102969\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 2017, aop","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16083","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"Malikova M, Voslarova E, Vecerek V","pdf":{"path":"http:\/\/data\/BUM_AOP_16083.pdf","title":"BUM_AOP_16083.pdf","description":"Non-invasive faecal monitoring of stress in captive and free-ranging wild animals"},"authors":[{"firstName":"M","middleName":"","lastName":"Malikova"},{"firstName":"E","middleName":"","lastName":"Voslarova"},{"firstName":"V","middleName":"","lastName":"Vecerek"}],"contentOptimised":"

Summary<\/strong>
Non-invasive monitoring of faecal metabolites of glucocorticoid hormones over the last two decades has become a frequently used method of assessing stress responses in wild animals. The number of studies focusing on this area of research is constantly growing, and also growing is the number of animals for which these methods have been described and validated in a variety of potentially stressful situations. The following text summarizes the advantages of non-invasive methods, particularly analysis of faecal samples, over invasive methods and their application in assessing stress in wild animals. Reference to species in which these methods have been successfully applied is given. Metabolism and excretion of glucocorticoid hormones and their elimination in the form of metabolites in faeces are described. Finally, the paper summarizes the principles of proper sample collection and their storage, and also discusses possible methods of analysis of those samples, which consists of extraction and immunoassay.<\/p>

Keywords:<\/strong>
mammals, faecal samples, steroids, cortisol metabolites, enzyme immunoassay<\/p>

Zusammenfassung<\/strong>
Das nichtinvasive Monitoring von Glucocorticoid-Metaboliten im Kot frei lebender Tiere wird in den zwei letzten Dekaden immer h\u00e4ufiger zum Beurteilen der Reaktion der Tiere auf Stress eingesetzt. Dieser Forschungsbereich ist deshalb im Wachstum begriffen, und auch die Anzahl an Tierarten nimmt zu, f\u00fcr die diese Methoden beschrieben und validiert wurden. Im nachfolgenden Text werden die Vorteile nichtinvasiver Methoden, insbesondere die Analyse von Kotproben, im Vergleich zu invasiven zusammengefasst sowie deren Anwendung beim Beurteilen von Stress bei frei lebenden Tieren erl\u00e4utert. Der Verweis auf Tierarten, bei denen diese Methoden erfolgreich angewandt wurden, wird angef\u00fchrt. In weiteren Passagen werden Metabolismus und Exkretion von Glucocorticoiden sowie deren Ausscheidung in Form von Metaboliten in den Kot beschrieben. Ferner werden die Grunds\u00e4tze f\u00fcr richtiges Sammeln und Lagern der Proben zusammengefasst und m\u00f6gliche Methoden f\u00fcr deren Analyse beschrieben, bestehend aus Extraktion und eigentlicher Immunanalyse.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
S\u00e4ugetiere, Kotproben, Steroidhormone, Kortisolmetaboliten, Enzymimmunoassay<\/p>","primaryLanguage":"englisch","summary":"Non-invasive monitoring of faecal metabolites of glucocorticoid hormones over the last two decades has become a frequently used method of assessing stress responses in wild animals. The number of studies focusing on this area of research is constantly growing, and also growing is the number of animals for which these methods have been described and validated in a variety of potentially stressful situations. The following text summarizes the advantages of non-invasive methods, particularly analysis of faecal samples, over invasive methods and their application in assessing stress in wild animals. Reference to species in which these methods have been successfully applied is given. Metabolism and excretion of glucocorticoid hormones and their elimination in the form of metabolites in faeces are described. Finally, the paper summarizes the principles of proper sample collection and their storage, and also discusses possible methods of analysis of those samples, which consists of extraction and immunoassay.<\/p>

","keywords":["mammals","faecal samples","steroids","cortisol metabolites","enzyme immunoassay"],"zusammenfassung":"Das nichtinvasive Monitoring von Glucocorticoid-Metaboliten im Kot frei lebender Tiere wird in den zwei letzten Dekaden immer h\u00e4ufiger zum Beurteilen der Reaktion der Tiere auf Stress eingesetzt. Dieser Forschungsbereich ist deshalb im Wachstum begriffen, und auch die Anzahl an Tierarten nimmt zu, f\u00fcr die diese Methoden beschrieben und validiert wurden. Im nachfolgenden Text werden die Vorteile nichtinvasiver Methoden, insbesondere die Analyse von Kotproben, im Vergleich zu invasiven zusammengefasst sowie deren Anwendung beim Beurteilen von Stress bei frei lebenden Tieren erl\u00e4utert. Der Verweis auf Tierarten, bei denen diese Methoden erfolgreich angewandt wurden, wird angef\u00fchrt. In weiteren Passagen werden Metabolismus und Exkretion von Glucocorticoiden sowie deren Ausscheidung in Form von Metaboliten in den Kot beschrieben. Ferner werden die Grunds\u00e4tze f\u00fcr richtiges Sammeln und Lagern der Proben zusammengefasst und m\u00f6gliche Methoden f\u00fcr deren Analyse beschrieben, bestehend aus Extraktion und eigentlicher Immunanalyse.<\/p>

","schluesselwoerter":["S\u00e4ugetiere","Kotproben","Steroidhormone","Kortisolmetaboliten","Enzymimmunoassay"],"translatedTitle":"Nichtinvasives Stressmonitoring anhand von Kotproben bei Wildtieren in Zoos und im Freiland","abstractE":"Non-invasive monitoring of faecal metabolites of glucocorticoid hormones over the last two decades has become a frequently used method of assessing stress responses in wild animals. The number of studies focusing on this area of research is constantly growing, and also growing is the number of animals for which these methods have been described and validated in a variety of potentially stressful situations. The following text summarizes the advantages of non-invasive methods, particularly analysis of faecal samples, over invasive methods and their application in assessing stress in wild animals. Reference to species in which these methods have been successfully applied is given. Metabolism and excretion of glucocorticoid hormones and their elimination in the form of metabolites in faeces are described. Finally, the paper summarizes the principles of proper sample collection and their storage, and also discusses possible methods of analysis of those samples, which consists of extraction and immunoassay.","date":{"year":2017,"date":"06\/2017","accepted":"2017-06-21"},"volume":"2017","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["non-invasive-faecal-monitoring-of-stress-in-captive-and-free-ranging-wild-animals\/150\/3130\/102969"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 06/2017 DO - 10.2376/0005-9366-16083 LA - English N2 - Non-invasive monitoring of faecal metabolites of glucocorticoid hormones over the last two decades has become a frequently used method of assessing stress responses in wild animals. The number of studies focusing on this area of research is constantly growing, and also growing is the number of animals for which these methods have been described and validated in a variety of potentially stressful situations. The following text summarizes the advantages of non-invasive methods, particularly analysis of faecal samples, over invasive methods and their application in assessing stress in wild animals. Reference to species in which these methods have been successfully applied is given. Metabolism and excretion of glucocorticoid hormones and their elimination in the form of metabolites in faeces are described. Finally, the paper summarizes the principles of proper sample collection and their storage, and also discusses possible methods of analysis of those samples, which consists of extraction and immunoassay. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 T1 - Non-invasive faecal monitoring of stress in captive and free-ranging wild animals T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Non-invasive faecal monitoring of stress in captive and free-ranging wild animals TT - Nichtinvasives Stressmonitoring anhand von Kotproben bei Wildtieren in Zoos und im Freiland VL - 130 SN - 0005-9366 ER -