TY - JOUR KW - Arcanobacterium abortisuis KW - Trueperella KW - 16S-23S rDNA intergenic spacer region KW - MALDI-TOF mass spectrometry AU - M Hijazin AU - H Ülbegi-Mohyla AU - J Alber AU - C Lämmler AU - A Hassan AU - M Timke AU - M Kostrzewa AU - E Prenger-Berninghoff AU - R Weiss AU - M Zschöck AB - In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":70067,"title":"Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS)","topline":"","teaserText":"In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic ...","content":"

Summary<\/span>
In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.

Keywords:<\/span>
Arcanobacterium abortisuis, Trueperella, 16S-23S rDNA intergenic spacer region, MALDI-TOF mass spectrometry


Zusammenfassung<\/span>
In den vorliegenden Untersuchungen konnten A. (T.) abortisuis-Kulturen, isoliert von Schwein und Rind, durch ph\u00e4notypische Eigenschaften, durch Polymerasekettenreaktion unter Vewendung von A. (T.) abortisuis 16S-23S rDNA intergenic spacer region-spezifischen Oligonukleotidprimern und durch Matrix-Assisted Laser Desorption Ionization-Time Of Flight Massenspektrometrie (MALDI-TOF MS) zuverl\u00e4ssig identifiziert werden. Letzteres k\u00f6nnte eine vielverprechende Methode zur schnellen und kosteng\u00fcnstigen Identifizierung dieser Spezies sein und dazu beitragen die Bedeutung von A. (T.) abortisuis bei Infektionen von Schwein und Rind, m\u00f6glicherweise auch bei anderen Tierarten und beim Mensch, zu verstehen.

Schl\u00fcsselw\u00f6rter:<\/span>
Arcanobacterium abortisuis, Trueperella, 16S-23S rDNA intergenic spacer region, MALDI-TOF Massenspektrometrie <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jan 10, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/arcanobacterium-abortisuis-trueperella-16s-23s-rdna-intergenic-spacer-region-maldi-tof-mass-spectrometry\/150\/3130\/70067"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/arcanobacterium-abortisuis-trueperella-16s-23s-rdna-intergenic-spacer-region-maldi-tof-mass-spectrometry\/150\/3130\/70067","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 1-2, 32-37 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-32","doiFirstPage":"32","doiLastPage":"37","doiTransmitted":true,"doiAuthor":"Hijazin M, \u00dclbegi-Mohyla H, Alber J, L\u00e4mmler C, Hassan AA, Timke M, Kostrzewa M, Prenger-Berninghoff E, Weiss R, Zsch\u00f6ck M","pdf":{"path":"http:\/\/data\/bmtw_2012_01_0032.pdf","title":"bmtw_2012_01_0032.pdf","description":"Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS)

"},"authors":[{"firstName":"M","middleName":"","lastName":"Hijazin"},{"firstName":"H","middleName":"","lastName":"\u00dclbegi-Mohyla"},{"firstName":"J","middleName":"","lastName":"Alber"},{"firstName":"C","middleName":"","lastName":"L\u00e4mmler"},{"firstName":"A","middleName":"A","lastName":"Hassan"},{"firstName":"M","middleName":"","lastName":"Timke"},{"firstName":"M","middleName":"","lastName":"Kostrzewa"},{"firstName":"E","middleName":"","lastName":"Prenger-Berninghoff"},{"firstName":"R","middleName":"","lastName":"Weiss"},{"firstName":"M","middleName":"","lastName":"Zsch\u00f6ck"}],"contentOptimised":"

Summary<\/strong>
In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.

Keywords:<\/strong>
Arcanobacterium abortisuis, Trueperella, 16S-23S rDNA intergenic spacer region, MALDI-TOF mass spectrometry


Zusammenfassung<\/strong>
In den vorliegenden Untersuchungen konnten A. (T.) abortisuis-Kulturen, isoliert von Schwein und Rind, durch ph\u00e4notypische Eigenschaften, durch Polymerasekettenreaktion unter Vewendung von A. (T.) abortisuis 16S-23S rDNA intergenic spacer region-spezifischen Oligonukleotidprimern und durch Matrix-Assisted Laser Desorption Ionization-Time Of Flight Massenspektrometrie (MALDI-TOF MS) zuverl\u00e4ssig identifiziert werden. Letzteres k\u00f6nnte eine vielverprechende Methode zur schnellen und kosteng\u00fcnstigen Identifizierung dieser Spezies sein und dazu beitragen die Bedeutung von A. (T.) abortisuis bei Infektionen von Schwein und Rind, m\u00f6glicherweise auch bei anderen Tierarten und beim Mensch, zu verstehen.

Schl\u00fcsselw\u00f6rter:<\/strong>
Arcanobacterium abortisuis, Trueperella, 16S-23S rDNA intergenic spacer region, MALDI-TOF Massenspektrometrie <\/p>","primaryLanguage":"englisch","summary":"In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.","keywords":["Arcanobacterium abortisuis","Trueperella","16S-23S rDNA intergenic spacer region","MALDI-TOF mass spectrometry"],"zusammenfassung":"In den vorliegenden Untersuchungen konnten A. (T.) abortisuis-Kulturen, isoliert von Schwein und Rind, durch ph\u00e4notypische Eigenschaften, durch Polymerasekettenreaktion unter Vewendung von A. (T.) abortisuis 16S-23S rDNA intergenic spacer region-spezifischen Oligonukleotidprimern und durch Matrix-Assisted Laser Desorption Ionization-Time Of Flight Massenspektrometrie (MALDI-TOF MS) zuverl\u00e4ssig identifiziert werden. Letzteres k\u00f6nnte eine vielverprechende Methode zur schnellen und kosteng\u00fcnstigen Identifizierung dieser Spezies sein und dazu beitragen die Bedeutung von A. (T.) abortisuis bei Infektionen von Schwein und Rind, m\u00f6glicherweise auch bei anderen Tierarten und beim Mensch, zu verstehen.","schluesselwoerter":["Arcanobacterium abortisuis","Trueperella","16S-23S rDNA intergenic spacer region","MALDI-TOF Massenspektrometrie"],"translatedTitle":"In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic ...","abstractE":"In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans.","date":{"year":2012,"date":"01\/2012","accepted":"2012-01-10"},"volume":"125","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"32-37","redirects":["arcanobacterium-abortisuis-trueperella-16s-23s-rdna-intergenic-spacer-region-maldi-tof-mass-spectrometry\/150\/3130\/70067"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 01/2012 DO - 10.2376/0005-9366-125-32 LA - English N2 - In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic spacer region specific oligonucleotide primer and by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS). The latter appeared to be a promising tool for fast and cost effective identification of this species and might help to elucidate the role A. (T.) abortisuis plays in infections of pigs, bovines, possibly other animals or humans. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2012 SP - 32 EP - 37 T1 - Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS) T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Identification of Arcanobacterium (Trueperella) abortisuis, a novel species of veterinary importance, by Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS) TT - In the present study A. (T.) abortisuis isolated from pigs and bovines could be reliably identified by determination of phenotypic properties, genotypically by polymerase chain reaction with the help of A. (T.) abortisuis 16S-23S rDNA intergenic ... VL - 125 SN - 0005-9366 ER -