TY - JOUR KW - Black soldier larvae KW - microbial characterization KW - microbial inactivation KW - HHP AU - C Marin AU - P Urbina AU - P Salvatierra AU - D Rodrigo AU - P Fernandez AU - A Martinez AB - A microbiological characterization of black soldier fly (Hermetia illucens) larvae as well as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis var. Typhimurium in these larvae by using High Hydrostatic Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu/g and an Enterobacteriaceae load of 4.29 log cfu/g. The presence of pathogenic microorganism varied: no Listeria spp. were found, but Salmonella spp and Escherichia (E.) coli were detected in the larvae extract at a contamination level of 4.04 log cfu/g and 3.84 log cfu/g, respectively. The total anaerobic bacteria load was 5.63 log cfu/g, but no sulfitereducing Clostridium were encountered. The mold and yeast level was 5.19 log cfu/g. HHP was effective against naturally contaminating yeasts and molds producing reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15 min). The inactivation level of Salmonella inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":110619,"title":"Effect of High Hydrostatic Pressure on natural contaminating microorganisms and inoculated Salmonella in Hermetia illucens larvae","topline":"","teaserText":"Auswirkungen des hydrostatischen Hochdrucks auf nat\u00fcrlich kontaminierende Mikroorganismen und inokulierte Salmonella in Hermetia illucens-Larven","content":"

Summary<\/span>
A microbiological characterization of black soldier fly (Hermetia illucens<\/span>) larvae as well as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis<\/span> var. Typhimurium in these larvae by using High Hydrostatic Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu\/g and an Enterobacteriaceae load of 4.29 log cfu\/g. The presence of pathogenic microorganism varied: no Listeria<\/span> spp. were found, but Salmonella<\/span> spp and Escherichia (E.) coli<\/span> were detected in the larvae extract at a contamination level of 4.04 log cfu\/g and 3.84 log cfu\/g, respectively. The total anaerobic bacteria load was 5.63 log cfu\/g, but no sulfitereducing Clostridium<\/span> were encountered. The mold and yeast level was 5.19 log cfu\/g. HHP was effective against naturally contaminating yeasts and molds producing reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15 min). The inactivation level of Salmonella<\/span> inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model.<\/p>

Keywords<\/span>
Black soldier larvae, microbial characterization, microbial inactivation, HHP<\/p>

Zusammenfassung<\/span>
Es wurden eine mikrobielle Charakterisierung von Larven der Schwarzen Soldatenfliege (Hermetia illucens<\/span>) sowie eine Inaktivierungsstudie nat\u00fcrlich kontaminierter Mikroorganismen und inokulierter Salmonella enteriditis<\/span> var. Typhimurium durch hydrostatischen Hochdruck (HHP; 300\u2013350 MPa, 1,5\u201315 min) dieser Larven durchgef\u00fchrt. Die aerobe mesophile Gesamtkeimzahl (AMB) belief sich auf 5.28 log KBE\/g, die Zahl der Enterobacteriaceae auf 4.29 log KBE\/g. Bez\u00fcglich pathogener Mikroorganismen wurden keine Listeria<\/span> spp. nachgewiesen, w\u00e4hrend Salmonella<\/span> und E. coli<\/span> mit 4.04 log KBE\/g bzw. 3.84 log\/g nachgewiesen werden konnten. Die Koloniezahl anaerober Bakterien lag bei 5.63 log KBE\/g, jedoch wurden keine Sulfit-reduzierenden Clostridien gefunden. Die Untersuchung auf Schimmelpilze und Hefen wies eine Zahl von 5.19 log KBE\/g auf. Die Behandlung mit HHP war wirksam gegen Schimmelpilze und Hefen und konnte deren Koloniezahl bei 350 MPa w\u00e4hrend 7 min um 3 log-Stufen verringern, w\u00e4hrend lediglich eine geringe Reduktion aerober Keime, selbst unter Anwendung des h\u00f6chsten Druckniveaus (350 MPa, 15 min), erreicht wurde. Die Inaktivierung der inokulierten Salmonella<\/span> variierte. Bei 350 MPa w\u00e4hrend 7 min konnte eine Reduktion um mehr als 3 log-Stufen erreicht werden. Die Inaktivierungskurven wurden verschiedenen mathematischen Modellen angepasst. Von den drei angewandten Inaktivierungsmodellen beschrieb das biphasische Modell den Inaktivierungsverlauf der Zellen am besten.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Schwarze Soldatenfliegenlarven, mikrobiologische Charakterisierung, mikrobielle Inaktivierung, HHP<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Feb 13, 2019 4:28:27 PM","oldUrls":["http:\/\/vetline.de\/effect-of-high-hydrostatic-pressure-on-natural-contaminating-microorganisms-and-inoculated-isalmonellai-in-ihermetia-illucensi-larvae\/150\/3130\/110619"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiDocumentUri":"https:\/\/vetline.de\/files\/smfiledata\/7\/4\/9\/0\/8\/6\/BMTW_AOP_18058_Martinez.pdf","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschc","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-18058","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"Marin C, Urbina P, Salvatierra P, Rodrigo D, Fernandez P, Martinez A","pdf":{"path":"http:\/\/data\/BMTW_AOP_18058_Martinez.pdf","title":"BMTW_AOP_18058_Martinez","description":"Effect of High Hydrostatic Pressure on natural contaminating microorganisms and inoculated Salmonella<\/i> in Hermetia illucens<\/i> larvae"},"authors":[{"firstName":"C","middleName":"","lastName":"Marin"},{"firstName":"P","middleName":"","lastName":"Urbina"},{"firstName":"P","middleName":"","lastName":"Salvatierra"},{"firstName":"D","middleName":"","lastName":"Rodrigo"},{"firstName":"P","middleName":"","lastName":"Fernandez"},{"firstName":"A","middleName":"","lastName":"Martinez"}],"contentOptimised":"

Summary<\/strong>
A microbiological characterization of black soldier fly (Hermetia illucens<\/em>) larvae as well as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis<\/em> var. Typhimurium in these larvae by using High Hydrostatic Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu\/g and an Enterobacteriaceae load of 4.29 log cfu\/g. The presence of pathogenic microorganism varied: no Listeria<\/em> spp. were found, but Salmonella<\/em> spp and Escherichia (E.) coli<\/em> were detected in the larvae extract at a contamination level of 4.04 log cfu\/g and 3.84 log cfu\/g, respectively. The total anaerobic bacteria load was 5.63 log cfu\/g, but no sulfitereducing Clostridium<\/em> were encountered. The mold and yeast level was 5.19 log cfu\/g. HHP was effective against naturally contaminating yeasts and molds producing reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15 min). The inactivation level of Salmonella<\/em> inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model.<\/p>

Keywords:<\/strong>
Black soldier larvae, microbial characterization, microbial inactivation, HHP<\/p>

Zusammenfassung<\/strong>
Es wurden eine mikrobielle Charakterisierung von Larven der Schwarzen Soldatenfliege (Hermetia illucens<\/em>) sowie eine Inaktivierungsstudie nat\u00fcrlich kontaminierter Mikroorganismen und inokulierter Salmonella enteriditis<\/em> var. Typhimurium durch hydrostatischen Hochdruck (HHP; 300\u2013350 MPa, 1,5\u201315 min) dieser Larven durchgef\u00fchrt. Die aerobe mesophile Gesamtkeimzahl (AMB) belief sich auf 5.28 log KBE\/g, die Zahl der Enterobacteriaceae auf 4.29 log KBE\/g. Bez\u00fcglich pathogener Mikroorganismen wurden keine Listeria<\/em> spp. nachgewiesen, w\u00e4hrend Salmonella<\/em> und E. coli<\/em> mit 4.04 log KBE\/g bzw. 3.84 log\/g nachgewiesen werden konnten. Die Koloniezahl anaerober Bakterien lag bei 5.63 log KBE\/g, jedoch wurden keine Sulfit-reduzierenden Clostridien gefunden. Die Untersuchung auf Schimmelpilze und Hefen wies eine Zahl von 5.19 log KBE\/g auf. Die Behandlung mit HHP war wirksam gegen Schimmelpilze und Hefen und konnte deren Koloniezahl bei 350 MPa w\u00e4hrend 7 min um 3 log-Stufen verringern, w\u00e4hrend lediglich eine geringe Reduktion aerober Keime, selbst unter Anwendung des h\u00f6chsten Druckniveaus (350 MPa, 15 min), erreicht wurde. Die Inaktivierung der inokulierten Salmonella<\/em> variierte. Bei 350 MPa w\u00e4hrend 7 min konnte eine Reduktion um mehr als 3 log-Stufen erreicht werden. Die Inaktivierungskurven wurden verschiedenen mathematischen Modellen angepasst. Von den drei angewandten Inaktivierungsmodellen beschrieb das biphasische Modell den Inaktivierungsverlauf der Zellen am besten.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Schwarze Soldatenfliegenlarven, mikrobiologische Charakterisierung, mikrobielle Inaktivierung, HHP<\/p>","primaryLanguage":"englisch","summary":"A microbiological characterization of black soldier fly (Hermetia illucens<\/em>) larvae as well as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis<\/em> var. Typhimurium in these larvae by using High Hydrostatic Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu\/g and an Enterobacteriaceae load of 4.29 log cfu\/g. The presence of pathogenic microorganism varied: no Listeria<\/em> spp. were found, but Salmonella<\/em> spp and Escherichia (E.) coli<\/em> were detected in the larvae extract at a contamination level of 4.04 log cfu\/g and 3.84 log cfu\/g, respectively. The total anaerobic bacteria load was 5.63 log cfu\/g, but no sulfitereducing Clostridium<\/em> were encountered. The mold and yeast level was 5.19 log cfu\/g. HHP was effective against naturally contaminating yeasts and molds producing reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15 min). The inactivation level of Salmonella<\/em> inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model.<\/p>

","keywords":["Black soldier larvae","microbial characterization","microbial inactivation","HHP"],"zusammenfassung":"Es wurden eine mikrobielle Charakterisierung von Larven der Schwarzen Soldatenfliege (Hermetia illucens<\/em>) sowie eine Inaktivierungsstudie nat\u00fcrlich kontaminierter Mikroorganismen und inokulierter Salmonella enteriditis<\/em> var. Typhimurium durch hydrostatischen Hochdruck (HHP; 300\u2013350 MPa, 1,5\u201315 min) dieser Larven durchgef\u00fchrt. Die aerobe mesophile Gesamtkeimzahl (AMB) belief sich auf 5.28 log KBE\/g, die Zahl der Enterobacteriaceae auf 4.29 log KBE\/g. Bez\u00fcglich pathogener Mikroorganismen wurden keine Listeria<\/em> spp. nachgewiesen, w\u00e4hrend Salmonella<\/em> und E. coli<\/em> mit 4.04 log KBE\/g bzw. 3.84 log\/g nachgewiesen werden konnten. Die Koloniezahl anaerober Bakterien lag bei 5.63 log KBE\/g, jedoch wurden keine Sulfit-reduzierenden Clostridien gefunden. Die Untersuchung auf Schimmelpilze und Hefen wies eine Zahl von 5.19 log KBE\/g auf. Die Behandlung mit HHP war wirksam gegen Schimmelpilze und Hefen und konnte deren Koloniezahl bei 350 MPa w\u00e4hrend 7 min um 3 log-Stufen verringern, w\u00e4hrend lediglich eine geringe Reduktion aerober Keime, selbst unter Anwendung des h\u00f6chsten Druckniveaus (350 MPa, 15 min), erreicht wurde. Die Inaktivierung der inokulierten Salmonella<\/em> variierte. Bei 350 MPa w\u00e4hrend 7 min konnte eine Reduktion um mehr als 3 log-Stufen erreicht werden. Die Inaktivierungskurven wurden verschiedenen mathematischen Modellen angepasst. Von den drei angewandten Inaktivierungsmodellen beschrieb das biphasische Modell den Inaktivierungsverlauf der Zellen am besten.<\/p>

","schluesselwoerter":["Schwarze Soldatenfliegenlarven","mikrobiologische Charakterisierung","mikrobielle Inaktivierung","HHP"],"translatedTitle":"Auswirkungen des hydrostatischen Hochdrucks auf nat\u00fcrlich kontaminierende Mikroorganismen und inokulierte Salmonella in Hermetia illucens-Larven","abstractE":"A microbiological characterization of black soldier fly (Hermetia illucens) larvae as well as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis var. Typhimurium in these larvae by using High Hydrostatic Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu\/g and an Enterobacteriaceae load of 4.29 log cfu\/g. The presence of pathogenic microorganism varied: no Listeria spp. were found, but Salmonella spp and Escherichia (E.) coli were detected in the larvae extract at a contamination level of 4.04 log cfu\/g and 3.84 log cfu\/g, respectively. The total anaerobic bacteria load was 5.63 log cfu\/g, but no sulfitereducing Clostridium were encountered. The mold and yeast level was 5.19 log cfu\/g. HHP was effective against naturally contaminating yeasts and molds producing reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15 min). The inactivation level of Salmonella inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model.","date":{"year":2019,"date":"02\/2019","accepted":"2019-02-13"},"volume":132,"openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["effect-of-high-hydrostatic-pressure-on-natural-contaminating-microorganisms-and-inoculated-isalmonellai-in-ihermetia-illucensi-larvae\/150\/3130\/110619"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 02/2019 DO - 10.2376/0005-9366-18058 LA - English N2 - A microbiological characterization of black soldier fly (Hermetia illucens) larvae as well as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis var. Typhimurium in these larvae by using High Hydrostatic Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu/g and an Enterobacteriaceae load of 4.29 log cfu/g. The presence of pathogenic microorganism varied: no Listeria spp. were found, but Salmonella spp and Escherichia (E.) coli were detected in the larvae extract at a contamination level of 4.04 log cfu/g and 3.84 log cfu/g, respectively. The total anaerobic bacteria load was 5.63 log cfu/g, but no sulfitereducing Clostridium were encountered. The mold and yeast level was 5.19 log cfu/g. HHP was effective against naturally contaminating yeasts and molds producing reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15 min). The inactivation level of Salmonella inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2019 T1 - Effect of High Hydrostatic Pressure on natural contaminating microorganisms and inoculated Salmonella in Hermetia illucens larvae T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Effect of High Hydrostatic Pressure on natural contaminating microorganisms and inoculated Salmonella in Hermetia illucens larvae TT - Auswirkungen des hydrostatischen Hochdrucks auf natürlich kontaminierende Mikroorganismen und inokulierte Salmonella in Hermetia illucens-Larven VL - 132 SN - 0005-9366 ER -