TY - JOUR KW - resistant microorganisms KW - MRSA KW - ESBL KW - livestock KW - environmental health KW - slurry AU - A Friese AU - J Schulz AU - H Laube AU - C v. Salviati AU - J Hartung AU - U Roesler AB - The occurrence of laMRSA (livestock-associated methicillin-resistant Staphylococcus aureus) and extended-spectrum amp;#946;-lactamase (ESBL) and/or plasmidmediated AmpC β-lactamase-producing (AmpC) Enterobacteriaceae in healthy livestock herds is known for some time. The spread of these bacteria in the environment is discussed critically. The object of this study was to determine the presence of these microorganisms in faeces of livestock as well as the discussion about a potential faecal emission. Therefore, faeces samples from 37 different MRSA positive livestock holdings were tested for MRSA. Furthermore, faeces samples from 50 farms with an unknown status regarding ESBL/AmpC-producing E. coli were screened for those resistant bacteria. LaMRSA was detected in samples of turkey (2/5, 40%) and broiler fattening farms (1/4, 25%) as well as in pig farms with higher detection frequencies in fattening farms (11/15, 73.3%) than in breeding farms (4/12, 33.3%). ESBL/AmpC-producing E. coli was found in all investigated eight broiler farms (100%), in nine out of 16 (56.3%) breeding pig as well as in six out of 10 (60%) dairy cattle herds and in seven of 16 (43.8%) fattening pig holdings. This presents the first detection of ESBL/AmpC-producing E. coli originating from healthy pigs, turkeys and broilers in Germany. In addition, samples of fertilized field surfaces were studied exemplarily for the presence of MRSA (n = 4) as well as ESBL/AmpC-producing E. coli (n = 2). Furthermore, slurry samples from four broiler and five pig farms were analysed for the latter. Both MRSA and ESBL/ AmpC-producing E. coli could be detected on the field surfaces, the last also in slurry samples. Faecal emissions from animal husbandry seem to be one possible route for the spread of these resistant microorganisms in the environment. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":69610,"title":"Faecal occurrence and emissions of livestock-associated methicillin-resistant Staphylococcus aureus (laMRSA) and ESBL\/AmpC-producing E. coli from animal farms in Germany","topline":"","teaserText":"","content":"

Summary<\/span>
The occurrence of laMRSA (livestock-associated methicillin-resistant Staphylococcus aureus<\/span>) and extended-spectrum amp;#946;-lactamase (ESBL) and\/or plasmidmediated AmpC \u03b2-lactamase-producing (AmpC) Enterobacteriaceae<\/span> in healthy livestock herds is known for some time. The spread of these bacteria in the environment is discussed critically. The object of this study was to determine the presence of these microorganisms in faeces of livestock as well as the discussion about a potential faecal emission. Therefore, faeces samples from 37 different MRSA positive livestock holdings were tested for MRSA. Furthermore, faeces samples from 50 farms with an unknown status regarding ESBL\/AmpC-producing E. coli<\/span> were screened for those resistant bacteria. LaMRSA was detected in samples of turkey (2\/5, 40%) and broiler fattening farms (1\/4, 25%) as well as in pig farms with higher detection frequencies in fattening farms (11\/15, 73.3%) than in breeding farms (4\/12, 33.3%). ESBL\/AmpC-producing E. coli<\/span> was found in all investigated eight broiler farms (100%), in nine out of 16 (56.3%) breeding pig as well as in six out of 10 (60%) dairy cattle herds and in seven of 16 (43.8%) fattening pig holdings. This presents the first detection of ESBL\/AmpC-producing E. coli<\/span> originating from healthy pigs, turkeys and broilers in Germany. In addition, samples of fertilized field surfaces were studied exemplarily for the presence of MRSA (n = 4) as well as ESBL\/AmpC-producing E. coli<\/span> (n = 2). Furthermore, slurry samples from four broiler and five pig farms were analysed for the latter. Both MRSA and ESBL\/ AmpC-producing E. coli <\/span>could be detected on the field surfaces, the last also in slurry samples. Faecal emissions from animal husbandry seem to be one possible route for the spread of these resistant microorganisms in the environment.

Keywords:<\/span>
resistant microorganisms, MRSA, ESBL, livestock, environmental health, slurry


Zusammenfassung<\/span>
Das Vorkommen von laMRSA (livestock-assoziierte Methicillin-resistente Staphylococcus aureus<\/span>) und ESBL (Extended Spectrum amp;#946;-Lactamase) und\/oder plasmidvermittelten AmpC-\u03b2-Lactamase-produzierenden (AmpC) Enterobacteriaceae in gesunden Nutztierbest\u00e4nden ist seit einiger Zeit bekannt. Eine m\u00f6gliche Ausbreitung dieser Keime in die Stallumgebung wird zunehmend kritisch in der \u00d6ffentlichkeit betrachtet. Ziel dieser Studie ist die Erfassung des Vorkommens dieser Mikroorganismen im Kot verschiedener Nutztiere sowie die Diskussion einer m\u00f6glichen Verbreitung der resistenten Bakterien \u00fcber f\u00e4kale Emissionen. Dazu wurden Sammelkotproben von 37 verschiedenen MRSA-positiven Nutztierbest\u00e4nden auf das Vorkommen von MRSA untersucht. Weiterhin wurden Kotproben aus 50 Best\u00e4nden mit unbekanntem Status bez\u00fcglich ESBL\/AmpC-produzierenden E. coli<\/span> auf diese Keime untersucht. MRSA konnten sowohl in einigen Proben der Putenbest\u00e4nde (2\/5, 40 %) und Broilermastbest\u00e4nde (1\/4, 25 %) als auch in denen der Schweinebest\u00e4nde, dabei mit h\u00f6heren Nachweisraten in Mast- (11\/15, 73,3 %) als in Zuchtbetrieben (4\/12, 33,3 %), gefunden werden. ESBL\/ AmpC-produzierende E. coli<\/span> wurden in allen untersuchten Broilermastbest\u00e4nden (8\/8, 100 %), in 9\/16 (56,3 %) der Schweinezucht- und 6\/10 (60 %) der Milchrinderbest\u00e4nde sowie in 7\/16 (43,8 %) der Schweinemastbest\u00e4nde detektiert. Dies stellt gleichzeitig den ersten Nachweis von ESBL\/AmpC-produzierenden E. col<\/span>i<\/span> bei gesunden Schweinen, Puten und Broilern in Deutschland dar. Zus\u00e4tzlich wurden exemplarisch Proben der Bodenoberfl\u00e4che von beg\u00fcllten Feldern ausgew\u00e4hlter Best\u00e4nde auf MRSA (n = 4) sowie ESBL\/AmpC-produzierende E. coli<\/span> (n = 2) untersucht. Weiterhin wurden G\u00fclleproben von vier Gefl\u00fcgel- und f\u00fcnf Schweinebest\u00e4nden hinsichtlich ESBL\/AmpC-produzierender E. coli<\/span> analysiert. Sowohl MRSA als auch ESBL\/AmpC-produzierende E. coli<\/span> konnten auf den ged\u00fcngten Fl\u00e4chen nachgewiesen werden, Letztere auch in den G\u00fclleproben. Das Ausbringen von F\u00e4kalien zur D\u00fcngung k\u00f6nnte somit einen m\u00f6glichen Weg des Austrags der resistenten Keime aus den Nutztierhaltungen darstellen.

Schl\u00fcsselw\u00f6rter:<\/span>
Resistente Mikroorganismen, MRSA, ESBL, Nutztierhaltung, Umwelthygiene, G\u00fclle <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Mar 7, 2013 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/resistant-microorganisms-mrsa-esbl-livestock-environmental-health-slurry\/150\/3130\/69610","http:\/\/vetline.de\/resistant-microorganisms-mrsa-esbl-livestock-environmental-health-slurry\/150\/3216\/69610"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/resistant-microorganisms-mrsa-esbl-livestock-environmental-health-slurry\/150\/3130\/69610","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 126: 03, 175 (2013)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-126-175","doiFirstPage":"175","doiLastPage":"180","doiTransmitted":true,"doiAuthor":"Friese A, Schulz J, Laube H, von Salviati C, Hartung J, Roesler U","pdf":{"path":"http:\/\/data\/bmtw_2013_03_0175.pdf","title":"bmtw - Faecal occurrence and emissions of livestock-associated methicillin-resistant Staphylococcus aureus","description":""},"authors":[{"firstName":"A","middleName":"","lastName":"Friese"},{"firstName":"J","middleName":"","lastName":"Schulz"},{"firstName":"H","middleName":"","lastName":"Laube"},{"firstName":"C","middleName":"","lastName":"von Salviati"},{"firstName":"J","middleName":"","lastName":"Hartung"},{"firstName":"U","middleName":"","lastName":"Roesler"}],"contentOptimised":"

Summary<\/strong>
The occurrence of laMRSA (livestock-associated methicillin-resistant Staphylococcus aureus<\/em>) and extended-spectrum amp;#946;-lactamase (ESBL) and\/or plasmidmediated AmpC \u03b2-lactamase-producing (AmpC) Enterobacteriaceae<\/em> in healthy livestock herds is known for some time. The spread of these bacteria in the environment is discussed critically. The object of this study was to determine the presence of these microorganisms in faeces of livestock as well as the discussion about a potential faecal emission. Therefore, faeces samples from 37 different MRSA positive livestock holdings were tested for MRSA. Furthermore, faeces samples from 50 farms with an unknown status regarding ESBL\/AmpC-producing E. coli<\/em> were screened for those resistant bacteria. LaMRSA was detected in samples of turkey (2\/5, 40%) and broiler fattening farms (1\/4, 25%) as well as in pig farms with higher detection frequencies in fattening farms (11\/15, 73.3%) than in breeding farms (4\/12, 33.3%). ESBL\/AmpC-producing E. coli<\/em> was found in all investigated eight broiler farms (100%), in nine out of 16 (56.3%) breeding pig as well as in six out of 10 (60%) dairy cattle herds and in seven of 16 (43.8%) fattening pig holdings. This presents the first detection of ESBL\/AmpC-producing E. coli<\/em> originating from healthy pigs, turkeys and broilers in Germany. In addition, samples of fertilized field surfaces were studied exemplarily for the presence of MRSA (n = 4) as well as ESBL\/AmpC-producing E. coli<\/em> (n = 2). Furthermore, slurry samples from four broiler and five pig farms were analysed for the latter. Both MRSA and ESBL\/ AmpC-producing E. coli <\/em>could be detected on the field surfaces, the last also in slurry samples. Faecal emissions from animal husbandry seem to be one possible route for the spread of these resistant microorganisms in the environment.

Keywords:<\/strong>
resistant microorganisms, MRSA, ESBL, livestock, environmental health, slurry


Zusammenfassung<\/strong>
Das Vorkommen von laMRSA (livestock-assoziierte Methicillin-resistente Staphylococcus aureus<\/em>) und ESBL (Extended Spectrum amp;#946;-Lactamase) und\/oder plasmidvermittelten AmpC-\u03b2-Lactamase-produzierenden (AmpC) Enterobacteriaceae in gesunden Nutztierbest\u00e4nden ist seit einiger Zeit bekannt. Eine m\u00f6gliche Ausbreitung dieser Keime in die Stallumgebung wird zunehmend kritisch in der \u00d6ffentlichkeit betrachtet. Ziel dieser Studie ist die Erfassung des Vorkommens dieser Mikroorganismen im Kot verschiedener Nutztiere sowie die Diskussion einer m\u00f6glichen Verbreitung der resistenten Bakterien \u00fcber f\u00e4kale Emissionen. Dazu wurden Sammelkotproben von 37 verschiedenen MRSA-positiven Nutztierbest\u00e4nden auf das Vorkommen von MRSA untersucht. Weiterhin wurden Kotproben aus 50 Best\u00e4nden mit unbekanntem Status bez\u00fcglich ESBL\/AmpC-produzierenden E. coli<\/em> auf diese Keime untersucht. MRSA konnten sowohl in einigen Proben der Putenbest\u00e4nde (2\/5, 40 %) und Broilermastbest\u00e4nde (1\/4, 25 %) als auch in denen der Schweinebest\u00e4nde, dabei mit h\u00f6heren Nachweisraten in Mast- (11\/15, 73,3 %) als in Zuchtbetrieben (4\/12, 33,3 %), gefunden werden. ESBL\/ AmpC-produzierende E. coli<\/em> wurden in allen untersuchten Broilermastbest\u00e4nden (8\/8, 100 %), in 9\/16 (56,3 %) der Schweinezucht- und 6\/10 (60 %) der Milchrinderbest\u00e4nde sowie in 7\/16 (43,8 %) der Schweinemastbest\u00e4nde detektiert. Dies stellt gleichzeitig den ersten Nachweis von ESBL\/AmpC-produzierenden E. col<\/em>i<\/em> bei gesunden Schweinen, Puten und Broilern in Deutschland dar. Zus\u00e4tzlich wurden exemplarisch Proben der Bodenoberfl\u00e4che von beg\u00fcllten Feldern ausgew\u00e4hlter Best\u00e4nde auf MRSA (n = 4) sowie ESBL\/AmpC-produzierende E. coli<\/em> (n = 2) untersucht. Weiterhin wurden G\u00fclleproben von vier Gefl\u00fcgel- und f\u00fcnf Schweinebest\u00e4nden hinsichtlich ESBL\/AmpC-produzierender E. coli<\/em> analysiert. Sowohl MRSA als auch ESBL\/AmpC-produzierende E. coli<\/em> konnten auf den ged\u00fcngten Fl\u00e4chen nachgewiesen werden, Letztere auch in den G\u00fclleproben. Das Ausbringen von F\u00e4kalien zur D\u00fcngung k\u00f6nnte somit einen m\u00f6glichen Weg des Austrags der resistenten Keime aus den Nutztierhaltungen darstellen.

Schl\u00fcsselw\u00f6rter:<\/strong>
Resistente Mikroorganismen, MRSA, ESBL, Nutztierhaltung, Umwelthygiene, G\u00fclle <\/p>","primaryLanguage":"englisch","summary":"The occurrence of laMRSA (livestock-associated methicillin-resistant Staphylococcus aureus<\/em>) and extended-spectrum amp;#946;-lactamase (ESBL) and\/or plasmidmediated AmpC \u03b2-lactamase-producing (AmpC) Enterobacteriaceae<\/em> in healthy livestock herds is known for some time. The spread of these bacteria in the environment is discussed critically. The object of this study was to determine the presence of these microorganisms in faeces of livestock as well as the discussion about a potential faecal emission. Therefore, faeces samples from 37 different MRSA positive livestock holdings were tested for MRSA. Furthermore, faeces samples from 50 farms with an unknown status regarding ESBL\/AmpC-producing E. coli<\/em> were screened for those resistant bacteria. LaMRSA was detected in samples of turkey (2\/5, 40%) and broiler fattening farms (1\/4, 25%) as well as in pig farms with higher detection frequencies in fattening farms (11\/15, 73.3%) than in breeding farms (4\/12, 33.3%). ESBL\/AmpC-producing E. coli<\/em> was found in all investigated eight broiler farms (100%), in nine out of 16 (56.3%) breeding pig as well as in six out of 10 (60%) dairy cattle herds and in seven of 16 (43.8%) fattening pig holdings. This presents the first detection of ESBL\/AmpC-producing E. coli<\/em> originating from healthy pigs, turkeys and broilers in Germany. In addition, samples of fertilized field surfaces were studied exemplarily for the presence of MRSA (n = 4) as well as ESBL\/AmpC-producing E. coli<\/em> (n = 2). Furthermore, slurry samples from four broiler and five pig farms were analysed for the latter. Both MRSA and ESBL\/ AmpC-producing E. coli <\/em>could be detected on the field surfaces, the last also in slurry samples. Faecal emissions from animal husbandry seem to be one possible route for the spread of these resistant microorganisms in the environment.","keywords":["resistant microorganisms","MRSA","ESBL","livestock","environmental health","slurry"],"zusammenfassung":"Das Vorkommen von laMRSA (livestock-assoziierte Methicillin-resistente Staphylococcus aureus<\/em>) und ESBL (Extended Spectrum amp;#946;-Lactamase) und\/oder plasmidvermittelten AmpC-\u03b2-Lactamase-produzierenden (AmpC) Enterobacteriaceae in gesunden Nutztierbest\u00e4nden ist seit einiger Zeit bekannt. Eine m\u00f6gliche Ausbreitung dieser Keime in die Stallumgebung wird zunehmend kritisch in der \u00d6ffentlichkeit betrachtet. Ziel dieser Studie ist die Erfassung des Vorkommens dieser Mikroorganismen im Kot verschiedener Nutztiere sowie die Diskussion einer m\u00f6glichen Verbreitung der resistenten Bakterien \u00fcber f\u00e4kale Emissionen. Dazu wurden Sammelkotproben von 37 verschiedenen MRSA-positiven Nutztierbest\u00e4nden auf das Vorkommen von MRSA untersucht. Weiterhin wurden Kotproben aus 50 Best\u00e4nden mit unbekanntem Status bez\u00fcglich ESBL\/AmpC-produzierenden E. coli<\/em> auf diese Keime untersucht. MRSA konnten sowohl in einigen Proben der Putenbest\u00e4nde (2\/5, 40 %) und Broilermastbest\u00e4nde (1\/4, 25 %) als auch in denen der Schweinebest\u00e4nde, dabei mit h\u00f6heren Nachweisraten in Mast- (11\/15, 73,3 %) als in Zuchtbetrieben (4\/12, 33,3 %), gefunden werden. ESBL\/ AmpC-produzierende E. coli<\/em> wurden in allen untersuchten Broilermastbest\u00e4nden (8\/8, 100 %), in 9\/16 (56,3 %) der Schweinezucht- und 6\/10 (60 %) der Milchrinderbest\u00e4nde sowie in 7\/16 (43,8 %) der Schweinemastbest\u00e4nde detektiert. Dies stellt gleichzeitig den ersten Nachweis von ESBL\/AmpC-produzierenden E. col<\/em>i<\/em> bei gesunden Schweinen, Puten und Broilern in Deutschland dar. Zus\u00e4tzlich wurden exemplarisch Proben der Bodenoberfl\u00e4che von beg\u00fcllten Feldern ausgew\u00e4hlter Best\u00e4nde auf MRSA (n = 4) sowie ESBL\/AmpC-produzierende E. coli<\/em> (n = 2) untersucht. Weiterhin wurden G\u00fclleproben von vier Gefl\u00fcgel- und f\u00fcnf Schweinebest\u00e4nden hinsichtlich ESBL\/AmpC-produzierender E. coli<\/em> analysiert. Sowohl MRSA als auch ESBL\/AmpC-produzierende E. coli<\/em> konnten auf den ged\u00fcngten Fl\u00e4chen nachgewiesen werden, Letztere auch in den G\u00fclleproben. Das Ausbringen von F\u00e4kalien zur D\u00fcngung k\u00f6nnte somit einen m\u00f6glichen Weg des Austrags der resistenten Keime aus den Nutztierhaltungen darstellen.","schluesselwoerter":["Resistente Mikroorganismen","MRSA","ESBL","Nutztierhaltung","Umwelthygiene","G\u00fclle"],"translatedTitle":"","abstractE":"The occurrence of laMRSA (livestock-associated methicillin-resistant Staphylococcus aureus) and extended-spectrum amp;#946;-lactamase (ESBL) and\/or plasmidmediated AmpC \u03b2-lactamase-producing (AmpC) Enterobacteriaceae in healthy livestock herds is known for some time. The spread of these bacteria in the environment is discussed critically. The object of this study was to determine the presence of these microorganisms in faeces of livestock as well as the discussion about a potential faecal emission. Therefore, faeces samples from 37 different MRSA positive livestock holdings were tested for MRSA. Furthermore, faeces samples from 50 farms with an unknown status regarding ESBL\/AmpC-producing E. coli were screened for those resistant bacteria. LaMRSA was detected in samples of turkey (2\/5, 40%) and broiler fattening farms (1\/4, 25%) as well as in pig farms with higher detection frequencies in fattening farms (11\/15, 73.3%) than in breeding farms (4\/12, 33.3%). ESBL\/AmpC-producing E. coli was found in all investigated eight broiler farms (100%), in nine out of 16 (56.3%) breeding pig as well as in six out of 10 (60%) dairy cattle herds and in seven of 16 (43.8%) fattening pig holdings. This presents the first detection of ESBL\/AmpC-producing E. coli originating from healthy pigs, turkeys and broilers in Germany. In addition, samples of fertilized field surfaces were studied exemplarily for the presence of MRSA (n = 4) as well as ESBL\/AmpC-producing E. coli (n = 2). Furthermore, slurry samples from four broiler and five pig farms were analysed for the latter. Both MRSA and ESBL\/ AmpC-producing E. coli could be detected on the field surfaces, the last also in slurry samples. Faecal emissions from animal husbandry seem to be one possible route for the spread of these resistant microorganisms in the environment.","date":{"year":2013,"date":"03\/2013","accepted":"2013-03-07"},"volume":"126","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"175-180","redirects":["resistant-microorganisms-mrsa-esbl-livestock-environmental-health-slurry\/150\/3130\/69610","resistant-microorganisms-mrsa-esbl-livestock-environmental-health-slurry\/150\/3216\/69610"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 03/2013 DO - 10.2376/0005-9366-126-175 LA - English N2 - The occurrence of laMRSA (livestock-associated methicillin-resistant Staphylococcus aureus) and extended-spectrum amp;#946;-lactamase (ESBL) and/or plasmidmediated AmpC β-lactamase-producing (AmpC) Enterobacteriaceae in healthy livestock herds is known for some time. The spread of these bacteria in the environment is discussed critically. The object of this study was to determine the presence of these microorganisms in faeces of livestock as well as the discussion about a potential faecal emission. Therefore, faeces samples from 37 different MRSA positive livestock holdings were tested for MRSA. Furthermore, faeces samples from 50 farms with an unknown status regarding ESBL/AmpC-producing E. coli were screened for those resistant bacteria. LaMRSA was detected in samples of turkey (2/5, 40%) and broiler fattening farms (1/4, 25%) as well as in pig farms with higher detection frequencies in fattening farms (11/15, 73.3%) than in breeding farms (4/12, 33.3%). ESBL/AmpC-producing E. coli was found in all investigated eight broiler farms (100%), in nine out of 16 (56.3%) breeding pig as well as in six out of 10 (60%) dairy cattle herds and in seven of 16 (43.8%) fattening pig holdings. This presents the first detection of ESBL/AmpC-producing E. coli originating from healthy pigs, turkeys and broilers in Germany. In addition, samples of fertilized field surfaces were studied exemplarily for the presence of MRSA (n = 4) as well as ESBL/AmpC-producing E. coli (n = 2). Furthermore, slurry samples from four broiler and five pig farms were analysed for the latter. Both MRSA and ESBL/ AmpC-producing E. coli could be detected on the field surfaces, the last also in slurry samples. Faecal emissions from animal husbandry seem to be one possible route for the spread of these resistant microorganisms in the environment. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2013 SP - 175 EP - 180 T1 - Faecal occurrence and emissions of livestock-associated methicillin-resistant Staphylococcus aureus (laMRSA) and ESBL/AmpC-producing E. coli from animal farms in Germany T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Faecal occurrence and emissions of livestock-associated methicillin-resistant Staphylococcus aureus (laMRSA) and ESBL/AmpC-producing E. coli from animal farms in Germany VL - 126 SN - 0005-9366 ER -