TY - JOUR AU - C Freuling AU - B Hoffmann AU - T Selhorst AU - F Conraths AU - J Kliemt AU - J Schatz AU - T Müller AB - Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":69555,"title":"New insights into the genetics of EBLV-1 from Germany","topline":"","teaserText":"","content":"

Summary<\/span>
Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before.

Keywords:<\/span>



Zusammenfassung<\/span>
Vorherige epidemiologische Studien \u00fcber EBLVs deuteten auf eine geografische Verteilung von EBLV-1 in Deutschland. Im Rahmen dieser Studie wurden 48 Fledermaustollwutvirusisolate aus Deutschland repr\u00e4sentativ ausgew\u00e4hlt und durch die direkte Sequenzierung des N-Gens sowie der nicht-translatierte Bereiche zwischen dem N- und P-Gen (NP-NTR) bzw. dem G- und L-Gen (GL-NTR) vergleichend charakterisiert. Die Ergebnisse best\u00e4tigten vorangegangene Studien an EBLV-1a-Isolaten, wonach die Sequenzen eine hohe Identit\u00e4t aufweisen. Es konnte jedoch gezeigt werden, dass die genetische Distanz mit der r\u00e4umlichen und zeitlichen korreliert ist. Zudem deuten die Ergebnisse darauf hin, dass die GL-NTR bei EBLV-1 nicht f\u00fcr vergleichende phylogenetische Studien geeignet zu sein scheint.
Interessanterweise konnten im NP-NTR bei zwei Isolaten eine 6 nt-Insertion bzw. bei einem Isolat eine 2 nt-Insertion nachgewiesen werden. Zudem wurde bei einem Isolat eine Deletion von 35 nt im GL-NTR festgestellt. Die Folgen dieser Mutationen auf virale Eigenschaften sind Gegenstand weiterer Untersuchungen, da diese bei Lyssaviren zuvor noch nicht beschrieben wurden.

Schl\u00fcsselw\u00f6rter:<\/span>
Europ\u00e4ische Fledermaustollwut, EBLV-1, Deutschland, Insertion, Deletion <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"May 4, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/eblv-1-phylogeny-bat-rabies-germany-insertion-deletion\/150\/3216\/69555","http:\/\/vetline.de\/eblv-1-phylogeny-bat-rabies-germany-insertion-deletion\/150\/3130\/69555"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/eblv-1-phylogeny-bat-rabies-germany-insertion-deletion\/150\/3130\/69555","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 5-6, 259-263 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-259","doiFirstPage":"259","doiLastPage":"263","doiTransmitted":true,"doiAuthor":"Freuling CM, Hoffmann B, Selhorst T, Conraths1 FJ, Kliemt J, Schatz J, M\u00fcller T","pdf":{"path":"http:\/\/data\/bmtw_2012_05_0259.pdf","title":"bmtw - New insights into the genetics of EBLV-1 from Germany ","description":""},"authors":[{"firstName":"C","middleName":"M","lastName":"Freuling"},{"firstName":"B","middleName":"","lastName":"Hoffmann"},{"firstName":"T","middleName":"","lastName":"Selhorst"},{"firstName":"F","middleName":"J","lastName":"Conraths1"},{"firstName":"J","middleName":"","lastName":"Kliemt"},{"firstName":"J","middleName":"","lastName":"Schatz"},{"firstName":"T","middleName":"","lastName":"M\u00fcller"}],"contentOptimised":"

Summary<\/strong>
Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before.

Keywords:<\/strong>



Zusammenfassung<\/strong>
Vorherige epidemiologische Studien \u00fcber EBLVs deuteten auf eine geografische Verteilung von EBLV-1 in Deutschland. Im Rahmen dieser Studie wurden 48 Fledermaustollwutvirusisolate aus Deutschland repr\u00e4sentativ ausgew\u00e4hlt und durch die direkte Sequenzierung des N-Gens sowie der nicht-translatierte Bereiche zwischen dem N- und P-Gen (NP-NTR) bzw. dem G- und L-Gen (GL-NTR) vergleichend charakterisiert. Die Ergebnisse best\u00e4tigten vorangegangene Studien an EBLV-1a-Isolaten, wonach die Sequenzen eine hohe Identit\u00e4t aufweisen. Es konnte jedoch gezeigt werden, dass die genetische Distanz mit der r\u00e4umlichen und zeitlichen korreliert ist. Zudem deuten die Ergebnisse darauf hin, dass die GL-NTR bei EBLV-1 nicht f\u00fcr vergleichende phylogenetische Studien geeignet zu sein scheint.
Interessanterweise konnten im NP-NTR bei zwei Isolaten eine 6 nt-Insertion bzw. bei einem Isolat eine 2 nt-Insertion nachgewiesen werden. Zudem wurde bei einem Isolat eine Deletion von 35 nt im GL-NTR festgestellt. Die Folgen dieser Mutationen auf virale Eigenschaften sind Gegenstand weiterer Untersuchungen, da diese bei Lyssaviren zuvor noch nicht beschrieben wurden.

Schl\u00fcsselw\u00f6rter:<\/strong>
Europ\u00e4ische Fledermaustollwut, EBLV-1, Deutschland, Insertion, Deletion <\/p>","primaryLanguage":"englisch","summary":"Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before.","keywords":"","zusammenfassung":"Vorherige epidemiologische Studien \u00fcber EBLVs deuteten auf eine geografische Verteilung von EBLV-1 in Deutschland. Im Rahmen dieser Studie wurden 48 Fledermaustollwutvirusisolate aus Deutschland repr\u00e4sentativ ausgew\u00e4hlt und durch die direkte Sequenzierung des N-Gens sowie der nicht-translatierte Bereiche zwischen dem N- und P-Gen (NP-NTR) bzw. dem G- und L-Gen (GL-NTR) vergleichend charakterisiert. Die Ergebnisse best\u00e4tigten vorangegangene Studien an EBLV-1a-Isolaten, wonach die Sequenzen eine hohe Identit\u00e4t aufweisen. Es konnte jedoch gezeigt werden, dass die genetische Distanz mit der r\u00e4umlichen und zeitlichen korreliert ist. Zudem deuten die Ergebnisse darauf hin, dass die GL-NTR bei EBLV-1 nicht f\u00fcr vergleichende phylogenetische Studien geeignet zu sein scheint.
Interessanterweise konnten im NP-NTR bei zwei Isolaten eine 6 nt-Insertion bzw. bei einem Isolat eine 2 nt-Insertion nachgewiesen werden. Zudem wurde bei einem Isolat eine Deletion von 35 nt im GL-NTR festgestellt. Die Folgen dieser Mutationen auf virale Eigenschaften sind Gegenstand weiterer Untersuchungen, da diese bei Lyssaviren zuvor noch nicht beschrieben wurden.","schluesselwoerter":["Europ\u00e4ische Fledermaustollwut","EBLV-1","Deutschland","Insertion","Deletion"],"translatedTitle":"","abstractE":"Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before.","date":{"year":2012,"date":"05\/2012","accepted":"2012-05-04"},"volume":"125","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"259-263","redirects":["eblv-1-phylogeny-bat-rabies-germany-insertion-deletion\/150\/3216\/69555","eblv-1-phylogeny-bat-rabies-germany-insertion-deletion\/150\/3130\/69555"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 05/2012 DO - 10.2376/0005-9366-125-259 LA - English N2 - Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2012 SP - 259 EP - 263 T1 - New insights into the genetics of EBLV-1 from Germany T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - New insights into the genetics of EBLV-1 from Germany VL - 125 SN - 0005-9366 ER -