TY - JOUR KW - Q fever KW - coxiellosis KW - chlamydiosis KW - Chlamydia KW - AU - M Runge AU - A Binder AU - U Schotte AU - M Ganter AB - The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever outbreaks during the last decades. There is only limited knowledge about the prevalence of both pathogens in sheep, although sheep are involved in almost all Q fever outbreaks in Germany. The aim of our study was to investigate the prevalence of both pathogens in flocks located in Lower Saxony, Germany, in correlation to the management form and abortion rate.Serum samples of 1714 sheep from 95 flocks located in Lower Saxony were investigated by ELISA. 2.7% of these samples were positive, 1.3% showed inconclusive results in the C. burnetii-ELISA. Elevated intra-flock seroprevalences were only detected in three migrating flocks. Chlamydia-specific antibodies could be detected in 15.1% serum samples of mainly shepherded and migrating flocks. In one of these flocks with a high intra-flock seroprevalence for C. burnetii (27%) and Chlamydia (44.9%), C. burnetii was detected in 21.6% of the placenta samples of normal births and in 12.5% of the colostrum samples by PCR. Aborted fetuses and the corresponding placentas were negative in C. burnetii-PCR, but in most of them and also in many other placenta samples Chl. abortus could be detected by PCR and DNA microarray.This survey shows a low overall prevalence of C. burnetii in sheep in Lower Saxony in the year 2004. However, three migrating flocks with a high intra-flock prevalence are localized in the southern parts of Lower Saxony. Spreading of C. burnetii could occur, because of the large radius of grazing of all three flocks. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":69509,"title":"Investigations concerning the prevalence of Coxiella burnetii and Chlamydia abortus in sheep in correlation with management systems and abortion rate in Lower Saxony in 2004","topline":"","teaserText":"The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever ...","content":"

Summary<\/span>
The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever outbreaks during the last decades. There is only limited knowledge about the prevalence of both pathogens in sheep, although sheep are involved in almost all Q fever outbreaks in Germany. The aim of our study was to investigate the prevalence of both pathogens in flocks located in Lower Saxony, Germany, in correlation to the management form and abortion rate.
Serum samples of 1714 sheep from 95 flocks located in Lower Saxony were investigated by ELISA. 2.7% of these samples were positive, 1.3% showed inconclusive results in the C. burnetii-ELISA. Elevated intra-flock seroprevalences were only detected in three migrating flocks. Chlamydia-specific antibodies could be detected in 15.1% serum samples of mainly shepherded and migrating flocks. In one of these flocks with a high intra-flock seroprevalence for C. burnetii (27%) and Chlamydia (44.9%), C. burnetii was detected in 21.6% of the placenta samples of normal births and in 12.5% of the colostrum samples by PCR. Aborted fetuses and the corresponding placentas were negative in C. burnetii-PCR, but in most of them and also in many other placenta samples Chl. abortus could be detected by PCR and DNA microarray.
This survey shows a low overall prevalence of C. burnetii in sheep in Lower Saxony in the year 2004. However, three migrating flocks with a high intra-flock prevalence are localized in the southern parts of Lower Saxony. Spreading of C. burnetii could occur, because of the large radius of grazing of all three flocks.

Keywords:<\/span>
Q fever, coxiellosis, chlamydiosis, Chlamydia,<\/span> zoonosis, pneumonia, abortion, DNA microarray


Zusammenfassung<\/span>
Infektionen mit den intrazellul\u00e4ren Bakterien Coxiella (C.) burnetii und Chlamydia (Chl.) abortus verursachen Aborte bei Schafen und Erkrankungen des Menschen. W\u00e4hrend Infektionen des Menschen mit Chl. abortus zu Erkrankungen einzelner Personen f\u00fchren, ist C. burnetii die Ursache zahlreicher Q-Fieber-Ausbr\u00fcche. \u00dcber die Pr\u00e4valenz dieser Bakterien bei Schafen ist wenig bekannt, obwohl Schafe an den meisten Q-Fieber-Ausbr\u00fcchen in Deutschland beteiligt waren. Das Ziel unserer Studie war es, das Vorkommen dieser beiden Bakterienspezies in nieders\u00e4chsischen Schafherden in Abh\u00e4ngigkeit von der Haltungsform und Abortrate zu untersuchen. Es wurden Serumproben von 1714 Schafen aus 95 Herden mittels ELISA untersucht. 2,7 % der Proben waren im C. burnetii-ELISA positiv, 1,3 % waren fraglich. Eine erh\u00f6hte Intra-Herden-Seropr\u00e4valenz wurde lediglich in drei Wanderschafherden nachgewiesen. Chlamydien-spezifische Antik\u00f6rper wiesen 15,1 % der Serumproben vor allem von H\u00fcte- und Wanderschafherden auf. In einer Herde mit einer Intra-Herden-Seropr\u00e4valenz von 27 % f\u00fcr C. burnetti und 44,9 % f\u00fcr Chlamydien wurde C. burnetii mittels PCR in 21,6 % der Plazentaproben der Normalgeburten und in 12,5 % Kolostrumproben, jedoch nicht in abortierten Feten und den entsprechenden Plazentaproben nachgewiesen. Vergleichsweise h\u00e4ufig wurde aber in den abortierten Feten, wie auch in vielen Plazentaproben Chl. abortus mittels PCR und DNA-Microarray nachgewiesen.
Insgesamt wurde eine niedrige Pr\u00e4valenz von <\/span>C. burnetii bei Schafen in Niedersachsen im Jahr 2004 festgestellt. Dennoch wurde bei drei im s\u00fcdlichen Niedersachsen angesiedelten Wanderschafherden eine hohe Intra-Herden-Pr\u00e4valenz nachgewiesen. Durch den gro\u00dfen Wanderradius k\u00f6nnte es zu einer weiteren Verbreitung von C. burnetii<\/span> kommen.

Schl\u00fcsselw\u00f6rter:<\/span>
Q-Fieber, Coxiellose, Chlamydiose, Chlamydien, Zoonose, Pneumonie, Abort, DNA Microarray <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Mar 12, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/q-fever-coxiellosis-chlamydiosis-chlamydia-zoonosis-pneumonia-abortion-dna-microarray\/150\/3130\/69509"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/q-fever-coxiellosis-chlamydiosis-chlamydia-zoonosis-pneumonia-abortion-dna-microarray\/150\/3130\/69509","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 3-4, 138-143 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-138","doiFirstPage":"138","doiLastPage":"143","doiTransmitted":true,"doiAuthor":"Runge M, Binder A, Schotte U, Ganter M","pdf":{"path":"http:\/\/data\/bmtw_2012_03_0138.pdf","title":"bmtw_2012_03_0138.pdf","description":"Investigations concerning the prevalence of Coxiella burnetii<\/i> and Chlamydia abortus<\/i> in sheep in correlation with management systems and abortion rate in Lower Saxony in 2004

"},"authors":[{"firstName":"M","middleName":"","lastName":"Runge"},{"firstName":"A","middleName":"","lastName":"Binder"},{"firstName":"U","middleName":"","lastName":"Schotte"},{"firstName":"M","middleName":"","lastName":"Ganter"}],"contentOptimised":"

Summary<\/strong>
The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever outbreaks during the last decades. There is only limited knowledge about the prevalence of both pathogens in sheep, although sheep are involved in almost all Q fever outbreaks in Germany. The aim of our study was to investigate the prevalence of both pathogens in flocks located in Lower Saxony, Germany, in correlation to the management form and abortion rate.
Serum samples of 1714 sheep from 95 flocks located in Lower Saxony were investigated by ELISA. 2.7% of these samples were positive, 1.3% showed inconclusive results in the C. burnetii-ELISA. Elevated intra-flock seroprevalences were only detected in three migrating flocks. Chlamydia-specific antibodies could be detected in 15.1% serum samples of mainly shepherded and migrating flocks. In one of these flocks with a high intra-flock seroprevalence for C. burnetii (27%) and Chlamydia (44.9%), C. burnetii was detected in 21.6% of the placenta samples of normal births and in 12.5% of the colostrum samples by PCR. Aborted fetuses and the corresponding placentas were negative in C. burnetii-PCR, but in most of them and also in many other placenta samples Chl. abortus could be detected by PCR and DNA microarray.
This survey shows a low overall prevalence of C. burnetii in sheep in Lower Saxony in the year 2004. However, three migrating flocks with a high intra-flock prevalence are localized in the southern parts of Lower Saxony. Spreading of C. burnetii could occur, because of the large radius of grazing of all three flocks.

Keywords:<\/strong>
Q fever, coxiellosis, chlamydiosis, Chlamydia,<\/strong> zoonosis, pneumonia, abortion, DNA microarray


Zusammenfassung<\/strong>
Infektionen mit den intrazellul\u00e4ren Bakterien Coxiella (C.) burnetii und Chlamydia (Chl.) abortus verursachen Aborte bei Schafen und Erkrankungen des Menschen. W\u00e4hrend Infektionen des Menschen mit Chl. abortus zu Erkrankungen einzelner Personen f\u00fchren, ist C. burnetii die Ursache zahlreicher Q-Fieber-Ausbr\u00fcche. \u00dcber die Pr\u00e4valenz dieser Bakterien bei Schafen ist wenig bekannt, obwohl Schafe an den meisten Q-Fieber-Ausbr\u00fcchen in Deutschland beteiligt waren. Das Ziel unserer Studie war es, das Vorkommen dieser beiden Bakterienspezies in nieders\u00e4chsischen Schafherden in Abh\u00e4ngigkeit von der Haltungsform und Abortrate zu untersuchen. Es wurden Serumproben von 1714 Schafen aus 95 Herden mittels ELISA untersucht. 2,7 % der Proben waren im C. burnetii-ELISA positiv, 1,3 % waren fraglich. Eine erh\u00f6hte Intra-Herden-Seropr\u00e4valenz wurde lediglich in drei Wanderschafherden nachgewiesen. Chlamydien-spezifische Antik\u00f6rper wiesen 15,1 % der Serumproben vor allem von H\u00fcte- und Wanderschafherden auf. In einer Herde mit einer Intra-Herden-Seropr\u00e4valenz von 27 % f\u00fcr C. burnetti und 44,9 % f\u00fcr Chlamydien wurde C. burnetii mittels PCR in 21,6 % der Plazentaproben der Normalgeburten und in 12,5 % Kolostrumproben, jedoch nicht in abortierten Feten und den entsprechenden Plazentaproben nachgewiesen. Vergleichsweise h\u00e4ufig wurde aber in den abortierten Feten, wie auch in vielen Plazentaproben Chl. abortus mittels PCR und DNA-Microarray nachgewiesen.
Insgesamt wurde eine niedrige Pr\u00e4valenz von <\/strong>C. burnetii bei Schafen in Niedersachsen im Jahr 2004 festgestellt. Dennoch wurde bei drei im s\u00fcdlichen Niedersachsen angesiedelten Wanderschafherden eine hohe Intra-Herden-Pr\u00e4valenz nachgewiesen. Durch den gro\u00dfen Wanderradius k\u00f6nnte es zu einer weiteren Verbreitung von C. burnetii<\/strong> kommen.

Schl\u00fcsselw\u00f6rter:<\/strong>
Q-Fieber, Coxiellose, Chlamydiose, Chlamydien, Zoonose, Pneumonie, Abort, DNA Microarray <\/p>","primaryLanguage":"englisch","summary":"The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever outbreaks during the last decades. There is only limited knowledge about the prevalence of both pathogens in sheep, although sheep are involved in almost all Q fever outbreaks in Germany. The aim of our study was to investigate the prevalence of both pathogens in flocks located in Lower Saxony, Germany, in correlation to the management form and abortion rate.
Serum samples of 1714 sheep from 95 flocks located in Lower Saxony were investigated by ELISA. 2.7% of these samples were positive, 1.3% showed inconclusive results in the C. burnetii-ELISA. Elevated intra-flock seroprevalences were only detected in three migrating flocks. Chlamydia-specific antibodies could be detected in 15.1% serum samples of mainly shepherded and migrating flocks. In one of these flocks with a high intra-flock seroprevalence for C. burnetii (27%) and Chlamydia (44.9%), C. burnetii was detected in 21.6% of the placenta samples of normal births and in 12.5% of the colostrum samples by PCR. Aborted fetuses and the corresponding placentas were negative in C. burnetii-PCR, but in most of them and also in many other placenta samples Chl. abortus could be detected by PCR and DNA microarray.
This survey shows a low overall prevalence of C. burnetii in sheep in Lower Saxony in the year 2004. However, three migrating flocks with a high intra-flock prevalence are localized in the southern parts of Lower Saxony. Spreading of C. burnetii could occur, because of the large radius of grazing of all three flocks.","keywords":["Q fever","coxiellosis","chlamydiosis","Chlamydia",""],"zusammenfassung":"Infektionen mit den intrazellul\u00e4ren Bakterien Coxiella (C.) burnetii und Chlamydia (Chl.) abortus verursachen Aborte bei Schafen und Erkrankungen des Menschen. W\u00e4hrend Infektionen des Menschen mit Chl. abortus zu Erkrankungen einzelner Personen f\u00fchren, ist C. burnetii die Ursache zahlreicher Q-Fieber-Ausbr\u00fcche. \u00dcber die Pr\u00e4valenz dieser Bakterien bei Schafen ist wenig bekannt, obwohl Schafe an den meisten Q-Fieber-Ausbr\u00fcchen in Deutschland beteiligt waren. Das Ziel unserer Studie war es, das Vorkommen dieser beiden Bakterienspezies in nieders\u00e4chsischen Schafherden in Abh\u00e4ngigkeit von der Haltungsform und Abortrate zu untersuchen. Es wurden Serumproben von 1714 Schafen aus 95 Herden mittels ELISA untersucht. 2,7 % der Proben waren im C. burnetii-ELISA positiv, 1,3 % waren fraglich. Eine erh\u00f6hte Intra-Herden-Seropr\u00e4valenz wurde lediglich in drei Wanderschafherden nachgewiesen. Chlamydien-spezifische Antik\u00f6rper wiesen 15,1 % der Serumproben vor allem von H\u00fcte- und Wanderschafherden auf. In einer Herde mit einer Intra-Herden-Seropr\u00e4valenz von 27 % f\u00fcr C. burnetti und 44,9 % f\u00fcr Chlamydien wurde C. burnetii mittels PCR in 21,6 % der Plazentaproben der Normalgeburten und in 12,5 % Kolostrumproben, jedoch nicht in abortierten Feten und den entsprechenden Plazentaproben nachgewiesen. Vergleichsweise h\u00e4ufig wurde aber in den abortierten Feten, wie auch in vielen Plazentaproben Chl. abortus mittels PCR und DNA-Microarray nachgewiesen.
Insgesamt wurde eine niedrige Pr\u00e4valenz von <\/strong>C. burnetii bei Schafen in Niedersachsen im Jahr 2004 festgestellt. Dennoch wurde bei drei im s\u00fcdlichen Niedersachsen angesiedelten Wanderschafherden eine hohe Intra-Herden-Pr\u00e4valenz nachgewiesen. Durch den gro\u00dfen Wanderradius k\u00f6nnte es zu einer weiteren Verbreitung von C. burnetii<\/strong> kommen.","schluesselwoerter":["Q-Fieber","Coxiellose","Chlamydiose","Chlamydien","Zoonose","Pneumonie","Abort","DNA Microarray"],"translatedTitle":"The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever ...","abstractE":"The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever outbreaks during the last decades. There is only limited knowledge about the prevalence of both pathogens in sheep, although sheep are involved in almost all Q fever outbreaks in Germany. The aim of our study was to investigate the prevalence of both pathogens in flocks located in Lower Saxony, Germany, in correlation to the management form and abortion rate.Serum samples of 1714 sheep from 95 flocks located in Lower Saxony were investigated by ELISA. 2.7% of these samples were positive, 1.3% showed inconclusive results in the C. burnetii-ELISA. Elevated intra-flock seroprevalences were only detected in three migrating flocks. Chlamydia-specific antibodies could be detected in 15.1% serum samples of mainly shepherded and migrating flocks. In one of these flocks with a high intra-flock seroprevalence for C. burnetii (27%) and Chlamydia (44.9%), C. burnetii was detected in 21.6% of the placenta samples of normal births and in 12.5% of the colostrum samples by PCR. Aborted fetuses and the corresponding placentas were negative in C. burnetii-PCR, but in most of them and also in many other placenta samples Chl. abortus could be detected by PCR and DNA microarray.This survey shows a low overall prevalence of C. burnetii in sheep in Lower Saxony in the year 2004. However, three migrating flocks with a high intra-flock prevalence are localized in the southern parts of Lower Saxony. Spreading of C. burnetii could occur, because of the large radius of grazing of all three flocks.","date":{"year":2012,"date":"03\/2012","accepted":"2012-03-12"},"volume":"125","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"138-143","redirects":["q-fever-coxiellosis-chlamydiosis-chlamydia-zoonosis-pneumonia-abortion-dna-microarray\/150\/3130\/69509"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 03/2012 DO - 10.2376/0005-9366-125-138 LA - English N2 - The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever outbreaks during the last decades. There is only limited knowledge about the prevalence of both pathogens in sheep, although sheep are involved in almost all Q fever outbreaks in Germany. The aim of our study was to investigate the prevalence of both pathogens in flocks located in Lower Saxony, Germany, in correlation to the management form and abortion rate.Serum samples of 1714 sheep from 95 flocks located in Lower Saxony were investigated by ELISA. 2.7% of these samples were positive, 1.3% showed inconclusive results in the C. burnetii-ELISA. Elevated intra-flock seroprevalences were only detected in three migrating flocks. Chlamydia-specific antibodies could be detected in 15.1% serum samples of mainly shepherded and migrating flocks. In one of these flocks with a high intra-flock seroprevalence for C. burnetii (27%) and Chlamydia (44.9%), C. burnetii was detected in 21.6% of the placenta samples of normal births and in 12.5% of the colostrum samples by PCR. Aborted fetuses and the corresponding placentas were negative in C. burnetii-PCR, but in most of them and also in many other placenta samples Chl. abortus could be detected by PCR and DNA microarray.This survey shows a low overall prevalence of C. burnetii in sheep in Lower Saxony in the year 2004. However, three migrating flocks with a high intra-flock prevalence are localized in the southern parts of Lower Saxony. Spreading of C. burnetii could occur, because of the large radius of grazing of all three flocks. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2012 SP - 138 EP - 143 T1 - Investigations concerning the prevalence of Coxiella burnetii and Chlamydia abortus in sheep in correlation with management systems and abortion rate in Lower Saxony in 2004 T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Investigations concerning the prevalence of Coxiella burnetii and Chlamydia abortus in sheep in correlation with management systems and abortion rate in Lower Saxony in 2004 TT - The intracellular bacteria Coxiella (C.) burnetii and Chlamydia (Chl.) abortus induce abortion in sheep and also affect humans. While Chl. abortus only infrequently infects humans, C. burnetii is the aetiological agent of numerous Q fever ... VL - 125 SN - 0005-9366 ER -