TY - JOUR KW - Staphylococcus KW - coagulase-positive KW - enterotoxin KW - chicken KW - Turkey KW - Broiler AU - A Marek AU - E Pyzik AU - D Stępień-Pyśniak AU - J Wilczyński AU - Ł Adaszek AB - The aim of the study was to characterize Staphylococcus strains isolated from broiler chickens and turkeys in Poland, with particular emphasis on their ability to produce four classical enterotoxins (SEA-SED). Among many Staphylococcus isolates species, 89 strains were characterized as potentially coagulase-positive species. Of these strains, 80 were identified as Staphylococcus aureus by Matrix Assisted Laser Desorption/Ionization (MALDI-TOF-MS) analysis. The other nine strains were identified as Staphylococcus delphini (4 strains), Staphylococcus hyicus (3 strains) and Staphylococcus schleiferi subsp. schleiferi (2 strains). All strains were tested for their ability to produce free coagulase. The results showed that 82 strains (92.13%) were coagulase-positive. All strains were also tested for toxin production by the reverse passive latex agglutination assay SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA). The results showed that only some of the S. aureus strains were able to produce enterotoxins in vitro ( 21 strains produced toxin D, eight strains toxin A, two toxin C and one toxin B). The presence of enterotoxigenic Staphylococcus aureus in samples taken from broiler chickens and turkeys poses a potential health risk to consumers. Although the literature indicates that other staphylococcal species may also produce enterotoxins, in our study none of the non-aureus strains exhibited this abilityKeywwords Staphylococcus, coagulase-positive, enterotoxin, chicken, turkey, broiler BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":101880,"title":"Phenotypic evaluation of the ability to produce enterotoxin in staphylococci isolated from broiler chickens and turkeys in Poland","topline":"","teaserText":"Ph\u00e4notypische Bewertung der F\u00e4higkeit zur Enterotoxin Produktion von Staphylokokken, die aus Masth\u00fchnern und Puten in Polen isoliert wurden","content":"

Summary<\/span>
The aim of the study was to characterize Staphylococcus strains isolated from broiler chickens and turkeys in Poland, with particular emphasis on their ability to produce four classical enterotoxins (SEA-SED). Among many Staphylococcus isolates species, 89 strains were characterized as potentially coagulase-positive species. Of these strains, 80 were identified as Staphylococcus aureus by Matrix Assisted Laser Desorption\/Ionization (MALDI-TOF-MS) analysis. The other nine strains were identified as Staphylococcus delphini (4 strains), Staphylococcus hyicus (3 strains) and Staphylococcus schleiferi subsp. schleiferi (2 strains). All strains were tested for their ability to produce free coagulase. The results showed that 82 strains (92.13%) were coagulase-positive. All strains were also tested for toxin production by the reverse passive latex agglutination assay SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA). The results showed that only some of the S. aureus strains were able to produce enterotoxins in vitro ( 21 strains produced toxin D, eight strains toxin A, two toxin C and one toxin B). The presence of enterotoxigenic Staphylococcus aureus in samples taken from broiler chickens and turkeys poses a potential health risk to consumers. Although the literature indicates that other staphylococcal species may also produce enterotoxins, in our study none of the non-aureus strains exhibited this ability<\/p>

Keywwords<\/span>
Staphylococcus, coagulase-positive, enterotoxin, chicken, turkey, broiler<\/p>

Zusammenfassung<\/span>
Ziel der Studie war es, Staphylococcus-St\u00e4mme, die aus Masth\u00fchnern und Puten in Polen isoliert wurden, zu charakterisieren, unter spezieller Ber\u00fccksichtigung ihrer F\u00e4higkeit, die vier klassischen Enterotoxine (SEA-SED) zu produzieren. Von den vielen Staphylococcus Isolaten wurden 89 St\u00e4mme als potentiell koagulasepositive Spezies charakterisiert. 80 von diesen St\u00e4mmen wurden mittels matrixunterst\u00fctzter Laser-Desorption\/-Ionisation (MALDI-TOF-MS) als Stapylococcus aureus identifiziert. Die anderen neun St\u00e4mme wurden als Staphylococcus delphini (4 St\u00e4mme), Staphylococcus hyicus (3 St\u00e4mme) und Staphylococcus schleiferi subsp. schleiferi (2 St\u00e4mme) identifiziert. Alle St\u00e4mme wurden auf ihre F\u00e4higkeit zur Produktion freier Koagulase getestet. Die Ergebnisse zeigten, dass 82 Isolate (92,13%) koagulasepositiv waren. Alle Isolate wurden auch auf Toxinproduktion mittels Reverse-Passive-Latex-Agglutinationstests SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA) getestet. Die Ergebnisse zeigten, dass nur einige der Staphylococcus aureus-St\u00e4mme Enterotoxine in vitro produzieren konnten (21 St\u00e4mme produzierten Toxin D, acht St\u00e4mme Toxin A, zwei Toxin C und einer Toxin B). Die Anwesenheit von enterotoxischem Staphylococcus aureus in Proben, die aus Masth\u00fchnern und Puten entnommen wurden, stellt ein potenzielles Gesundheitsrisiko f\u00fcr die Verbraucher dar. Trotz Hinweisen in Publikationen, dass andere Staphylokokken-Arten auch Enterotoxine produzieren k\u00f6nnen, zeigten in unserer Studie au\u00dfer Staphylococcus aureus keine anderen St\u00e4mme diese F\u00e4higkeit.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Staphylococcus, Koagulase-positiv, Enterotoxin, Huhn, Pute, Broiler<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Apr 13, 2017 9:25:17 AM","oldUrls":["http:\/\/vetline.de\/phenotypic-evaluation-of-the-ability-to-produce-enterotoxin-in-staphylococci-isolated-from-broiler-chickens-and-turkeys-in-poland\/150\/3130\/101880"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/phenotypic-evaluation-of-the-ability-to-produce-enterotoxin-in-staphylococci-isolated-from-broiler-chickens-and-turkeys-in-poland\/150\/3130\/101880\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 2017","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16050","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"Marek A, Pyzik E, St\u0119pie\u0144-Py\u015bniak D, Wilczy\u0144ski J, Adaszek \u0141","pdf":{"path":"http:\/\/data\/BMW_AOP_16050.pdf","title":"BMW_AOP_16050.pdf","description":"Phenotypic evaluation of the ability to produce enterotoxin in staphylococci isolated from broiler chickens and turkeys in Poland"},"authors":[{"firstName":"A","middleName":"","lastName":"Marek"},{"firstName":"E","middleName":"","lastName":"Pyzik"},{"firstName":"D","middleName":"","lastName":"St\u0119pie\u0144-Py\u015bniak"},{"firstName":"J","middleName":"","lastName":"Wilczy\u0144ski"},{"firstName":"\u0141","middleName":"","lastName":"Adaszek"}],"contentOptimised":"

Summary<\/strong>
The aim of the study was to characterize Staphylococcus strains isolated from broiler chickens and turkeys in Poland, with particular emphasis on their ability to produce four classical enterotoxins (SEA-SED). Among many Staphylococcus isolates species, 89 strains were characterized as potentially coagulase-positive species. Of these strains, 80 were identified as Staphylococcus aureus by Matrix Assisted Laser Desorption\/Ionization (MALDI-TOF-MS) analysis. The other nine strains were identified as Staphylococcus delphini (4 strains), Staphylococcus hyicus (3 strains) and Staphylococcus schleiferi subsp. schleiferi (2 strains). All strains were tested for their ability to produce free coagulase. The results showed that 82 strains (92.13%) were coagulase-positive. All strains were also tested for toxin production by the reverse passive latex agglutination assay SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA). The results showed that only some of the S. aureus strains were able to produce enterotoxins in vitro ( 21 strains produced toxin D, eight strains toxin A, two toxin C and one toxin B). The presence of enterotoxigenic Staphylococcus aureus in samples taken from broiler chickens and turkeys poses a potential health risk to consumers. Although the literature indicates that other staphylococcal species may also produce enterotoxins, in our study none of the non-aureus strains exhibited this ability<\/p>

Keywwords<\/strong>
Staphylococcus, coagulase-positive, enterotoxin, chicken, turkey, broiler<\/p>

Zusammenfassung<\/strong>
Ziel der Studie war es, Staphylococcus-St\u00e4mme, die aus Masth\u00fchnern und Puten in Polen isoliert wurden, zu charakterisieren, unter spezieller Ber\u00fccksichtigung ihrer F\u00e4higkeit, die vier klassischen Enterotoxine (SEA-SED) zu produzieren. Von den vielen Staphylococcus Isolaten wurden 89 St\u00e4mme als potentiell koagulasepositive Spezies charakterisiert. 80 von diesen St\u00e4mmen wurden mittels matrixunterst\u00fctzter Laser-Desorption\/-Ionisation (MALDI-TOF-MS) als Stapylococcus aureus identifiziert. Die anderen neun St\u00e4mme wurden als Staphylococcus delphini (4 St\u00e4mme), Staphylococcus hyicus (3 St\u00e4mme) und Staphylococcus schleiferi subsp. schleiferi (2 St\u00e4mme) identifiziert. Alle St\u00e4mme wurden auf ihre F\u00e4higkeit zur Produktion freier Koagulase getestet. Die Ergebnisse zeigten, dass 82 Isolate (92,13%) koagulasepositiv waren. Alle Isolate wurden auch auf Toxinproduktion mittels Reverse-Passive-Latex-Agglutinationstests SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA) getestet. Die Ergebnisse zeigten, dass nur einige der Staphylococcus aureus-St\u00e4mme Enterotoxine in vitro produzieren konnten (21 St\u00e4mme produzierten Toxin D, acht St\u00e4mme Toxin A, zwei Toxin C und einer Toxin B). Die Anwesenheit von enterotoxischem Staphylococcus aureus in Proben, die aus Masth\u00fchnern und Puten entnommen wurden, stellt ein potenzielles Gesundheitsrisiko f\u00fcr die Verbraucher dar. Trotz Hinweisen in Publikationen, dass andere Staphylokokken-Arten auch Enterotoxine produzieren k\u00f6nnen, zeigten in unserer Studie au\u00dfer Staphylococcus aureus keine anderen St\u00e4mme diese F\u00e4higkeit.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Staphylococcus, Koagulase-positiv, Enterotoxin, Huhn, Pute, Broiler<\/p>","primaryLanguage":"englisch","summary":"The aim of the study was to characterize Staphylococcus strains isolated from broiler chickens and turkeys in Poland, with particular emphasis on their ability to produce four classical enterotoxins (SEA-SED). Among many Staphylococcus isolates species, 89 strains were characterized as potentially coagulase-positive species. Of these strains, 80 were identified as Staphylococcus aureus by Matrix Assisted Laser Desorption\/Ionization (MALDI-TOF-MS) analysis. The other nine strains were identified as Staphylococcus delphini (4 strains), Staphylococcus hyicus (3 strains) and Staphylococcus schleiferi subsp. schleiferi (2 strains). All strains were tested for their ability to produce free coagulase. The results showed that 82 strains (92.13%) were coagulase-positive. All strains were also tested for toxin production by the reverse passive latex agglutination assay SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA). The results showed that only some of the S. aureus strains were able to produce enterotoxins in vitro ( 21 strains produced toxin D, eight strains toxin A, two toxin C and one toxin B). The presence of enterotoxigenic Staphylococcus aureus in samples taken from broiler chickens and turkeys poses a potential health risk to consumers. Although the literature indicates that other staphylococcal species may also produce enterotoxins, in our study none of the non-aureus strains exhibited this ability<\/p>

Keywwords<\/strong>
Staphylococcus, coagulase-positive, enterotoxin, chicken, turkey, broiler<\/p>

","zusammenfassung":"Ziel der Studie war es, Staphylococcus-St\u00e4mme, die aus Masth\u00fchnern und Puten in Polen isoliert wurden, zu charakterisieren, unter spezieller Ber\u00fccksichtigung ihrer F\u00e4higkeit, die vier klassischen Enterotoxine (SEA-SED) zu produzieren. Von den vielen Staphylococcus Isolaten wurden 89 St\u00e4mme als potentiell koagulasepositive Spezies charakterisiert. 80 von diesen St\u00e4mmen wurden mittels matrixunterst\u00fctzter Laser-Desorption\/-Ionisation (MALDI-TOF-MS) als Stapylococcus aureus identifiziert. Die anderen neun St\u00e4mme wurden als Staphylococcus delphini (4 St\u00e4mme), Staphylococcus hyicus (3 St\u00e4mme) und Staphylococcus schleiferi subsp. schleiferi (2 St\u00e4mme) identifiziert. Alle St\u00e4mme wurden auf ihre F\u00e4higkeit zur Produktion freier Koagulase getestet. Die Ergebnisse zeigten, dass 82 Isolate (92,13%) koagulasepositiv waren. Alle Isolate wurden auch auf Toxinproduktion mittels Reverse-Passive-Latex-Agglutinationstests SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA) getestet. Die Ergebnisse zeigten, dass nur einige der Staphylococcus aureus-St\u00e4mme Enterotoxine in vitro produzieren konnten (21 St\u00e4mme produzierten Toxin D, acht St\u00e4mme Toxin A, zwei Toxin C und einer Toxin B). Die Anwesenheit von enterotoxischem Staphylococcus aureus in Proben, die aus Masth\u00fchnern und Puten entnommen wurden, stellt ein potenzielles Gesundheitsrisiko f\u00fcr die Verbraucher dar. Trotz Hinweisen in Publikationen, dass andere Staphylokokken-Arten auch Enterotoxine produzieren k\u00f6nnen, zeigten in unserer Studie au\u00dfer Staphylococcus aureus keine anderen St\u00e4mme diese F\u00e4higkeit.<\/p>

","schluesselwoerter":["Staphylococcus","Koagulase-positiv","Enterotoxin","Huhn","Pute","Broiler"],"translatedTitle":"Ph\u00e4notypische Bewertung der F\u00e4higkeit zur Enterotoxin Produktion von Staphylokokken, die aus Masth\u00fchnern und Puten in Polen isoliert wurden","abstractE":"The aim of the study was to characterize Staphylococcus strains isolated from broiler chickens and turkeys in Poland, with particular emphasis on their ability to produce four classical enterotoxins (SEA-SED). Among many Staphylococcus isolates species, 89 strains were characterized as potentially coagulase-positive species. Of these strains, 80 were identified as Staphylococcus aureus by Matrix Assisted Laser Desorption\/Ionization (MALDI-TOF-MS) analysis. The other nine strains were identified as Staphylococcus delphini (4 strains), Staphylococcus hyicus (3 strains) and Staphylococcus schleiferi subsp. schleiferi (2 strains). All strains were tested for their ability to produce free coagulase. The results showed that 82 strains (92.13%) were coagulase-positive. All strains were also tested for toxin production by the reverse passive latex agglutination assay SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA). The results showed that only some of the S. aureus strains were able to produce enterotoxins in vitro ( 21 strains produced toxin D, eight strains toxin A, two toxin C and one toxin B). The presence of enterotoxigenic Staphylococcus aureus in samples taken from broiler chickens and turkeys poses a potential health risk to consumers. Although the literature indicates that other staphylococcal species may also produce enterotoxins, in our study none of the non-aureus strains exhibited this abilityKeywwords Staphylococcus, coagulase-positive, enterotoxin, chicken, turkey, broiler","date":{"year":2017,"date":"04\/2017","accepted":"2017-04-13"},"volume":"2017","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["phenotypic-evaluation-of-the-ability-to-produce-enterotoxin-in-staphylococci-isolated-from-broiler-chickens-and-turkeys-in-poland\/150\/3130\/101880"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 04/2017 DO - 10.2376/0005-9366-16050 LA - English N2 - The aim of the study was to characterize Staphylococcus strains isolated from broiler chickens and turkeys in Poland, with particular emphasis on their ability to produce four classical enterotoxins (SEA-SED). Among many Staphylococcus isolates species, 89 strains were characterized as potentially coagulase-positive species. Of these strains, 80 were identified as Staphylococcus aureus by Matrix Assisted Laser Desorption/Ionization (MALDI-TOF-MS) analysis. The other nine strains were identified as Staphylococcus delphini (4 strains), Staphylococcus hyicus (3 strains) and Staphylococcus schleiferi subsp. schleiferi (2 strains). All strains were tested for their ability to produce free coagulase. The results showed that 82 strains (92.13%) were coagulase-positive. All strains were also tested for toxin production by the reverse passive latex agglutination assay SET-RPLA-TD0900, (Thermo ScientificTM, Walthan, USA). The results showed that only some of the S. aureus strains were able to produce enterotoxins in vitro ( 21 strains produced toxin D, eight strains toxin A, two toxin C and one toxin B). The presence of enterotoxigenic Staphylococcus aureus in samples taken from broiler chickens and turkeys poses a potential health risk to consumers. Although the literature indicates that other staphylococcal species may also produce enterotoxins, in our study none of the non-aureus strains exhibited this abilityKeywwords Staphylococcus, coagulase-positive, enterotoxin, chicken, turkey, broiler PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 T1 - Phenotypic evaluation of the ability to produce enterotoxin in staphylococci isolated from broiler chickens and turkeys in Poland T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Phenotypic evaluation of the ability to produce enterotoxin in staphylococci isolated from broiler chickens and turkeys in Poland TT - Phänotypische Bewertung der Fähigkeit zur Enterotoxin-Produktion von Staphylokokken, die aus Masthühnern und Puten in Polen isoliert wurden VL - 130 SN - 0005-9366 ER -