TY - JOUR KW - BHV-1 KW - IBR-gE antibody KW - pool milk KW - milk concentration AU - C Schroeder AU - S Horner AU - N Bürger AU - C Engemann AU - U Bange AU - E Knoop AU - J Gabert AB - The low sensitivity of the IBR-gE ELISA compared to other diagnostic ELISA tests for IBR is a major disadvantage of IBR control programmes based on IBR marker vaccination. Therefore the IBR-gE ELISA is not generally recommended for testing pooled or bulk milk samples. The aim of this study was to determine the performance of a commercially available kit for concentrating and purifying antibodies in milk in order to improve the sensitivity of detecting IBR-gE antibody positive cows from pooled and bulk milk samples. A single IBR-gE positive cow is likely to remain undetected in a pool of 49 negative milk samples without concentration. By contrast, the bulk milk concentration procedure improved sensitivity from 5.4% to 75.7% in a positive herd. Milk samples with a high or moderate positive signal are more likely to be detected after pool milk concentration compared to weak positive samples. Whereas a follow up study involving a monthly testing of bulk milk samples from three marker vaccinated IBR-gE negative herds over a period of seven months yielded negative results each month, bulk milk from a herd containing BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":68992,"title":"Improving the sensitivity of the IBR-gE ELISA for testing IBR marker vaccinated cows from bulk milk","topline":"","teaserText":"","content":"

Summary<\/span>
The low sensitivity of the IBR-gE ELISA compared to other diagnostic ELISA tests for IBR is a major disadvantage of IBR control programmes based on IBR marker vaccination. Therefore the IBR-gE ELISA is not generally recommended for testing pooled or bulk milk samples. The aim of this study was to determine the performance of a commercially available kit for concentrating and purifying antibodies in milk in order to improve the sensitivity of detecting IBR-gE antibody positive cows from pooled and bulk milk samples.
A single IBR-gE positive cow is likely to remain undetected in a pool of 49 negative milk samples without concentration. By contrast, the bulk milk concentration procedure improved sensitivity from 5.4% to 75.7% in a positive herd. Milk samples with a high or moderate positive signal are more likely to be detected after pool milk concentration compared to weak positive samples.
Whereas a follow up study involving a monthly testing of bulk milk samples from three marker vaccinated IBR-gE negative herds over a period of seven months yielded negative results each month, bulk milk from a herd containing
Keywords:<\/span>
BHV-1, IBR-gE antibody, pool milk, milk concentration


Zusammenfassung<\/span>
Der IBR-gE-ELISA hat, im Vergleich mit anderen diagnostischen Tests zum Nachweis von IBR, eine geringe Sensitivit\u00e4t. Dies stellt einen deutlichen Nachteil bei IBR-Bek\u00e4mpfungsverfahren dar, die auf Markerimpfung beruhen, weshalb dieser Test im Allgemeinen nicht f\u00fcr die Untersuchung von Tankmilchproben empfohlen wird. Ziel dieser Studie war es, die Eignung eines kommerziell verf\u00fcgbaren Kits zur Anreicherung und Reinigung von Antik\u00f6rpern aus Milchproben zur Verbesserung der Sensitivit\u00e4t beim Nachweis IBR-gE-positiver K\u00fche aus Pool- und Tankmilchproben zu pr\u00fcfen.
Durch die Anreicherung von Poolmilchproben konnte die Sensitivit\u00e4t beim Nachweis einer IBR-gE-positiven Milch in Pools mit 49 negativen Milchproben von 5,4 % ohne Anreicherung auf 75,7 % nach Anreicherung erh\u00f6ht werden. Bei deutlich positiven und mittelgradig positiven Milchproben gelingt der Nachweis im Pool sicherer als bei schwach positiven Proben.
In einer Verlaufsstudie mit monatlicher Untersuchung von Tankmilchproben von drei IBR-gE-negativen, mit einem Marker-Impfstoff vakzinierten Herden sowie einer Herde mit
Schl\u00fcsselw\u00f6rter:<\/span>
BHV-1, IBR-gE-Antik\u00f6rper, Poolmilch, Milchkonzentrierung <\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Feb 24, 2012 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/bhv-1-ibr-ge-antibody-pool-milk-milk-concentration\/150\/3216\/68992","http:\/\/vetline.de\/bhv-1-ibr-ge-antibody-pool-milk-milk-concentration\/150\/3130\/68992"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/bhv-1-ibr-ge-antibody-pool-milk-milk-concentration\/150\/3130\/68992","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 125: 7-8, 290-296 (2012)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-125-290","doiFirstPage":"290","doiLastPage":"296","doiTransmitted":true,"doiAuthor":"Schroeder C, Horner S, B\u00fcrger N, Engemann C, Bange U, Knoop EV, Gabert J","pdf":{"path":"http:\/\/data\/bmtw_2012_07_0290.pdf","title":"bmtw - Improving the sensitivity of the IBR-gE ELISA for testing IBR marker vaccinated cows from bulk milk","description":""},"authors":[{"firstName":"C","middleName":"","lastName":"Schroeder"},{"firstName":"S","middleName":"","lastName":"Horner"},{"firstName":"N","middleName":"","lastName":"B\u00fcrger"},{"firstName":"C","middleName":"","lastName":"Engemann"},{"firstName":"U","middleName":"","lastName":"Bange"},{"firstName":"E","middleName":"V","lastName":"Knoop"},{"firstName":"J","middleName":"","lastName":"Gabert"}],"contentOptimised":"

Summary<\/strong>
The low sensitivity of the IBR-gE ELISA compared to other diagnostic ELISA tests for IBR is a major disadvantage of IBR control programmes based on IBR marker vaccination. Therefore the IBR-gE ELISA is not generally recommended for testing pooled or bulk milk samples. The aim of this study was to determine the performance of a commercially available kit for concentrating and purifying antibodies in milk in order to improve the sensitivity of detecting IBR-gE antibody positive cows from pooled and bulk milk samples.
A single IBR-gE positive cow is likely to remain undetected in a pool of 49 negative milk samples without concentration. By contrast, the bulk milk concentration procedure improved sensitivity from 5.4% to 75.7% in a positive herd. Milk samples with a high or moderate positive signal are more likely to be detected after pool milk concentration compared to weak positive samples.
Whereas a follow up study involving a monthly testing of bulk milk samples from three marker vaccinated IBR-gE negative herds over a period of seven months yielded negative results each month, bulk milk from a herd containing
Keywords:<\/strong>
BHV-1, IBR-gE antibody, pool milk, milk concentration


Zusammenfassung<\/strong>
Der IBR-gE-ELISA hat, im Vergleich mit anderen diagnostischen Tests zum Nachweis von IBR, eine geringe Sensitivit\u00e4t. Dies stellt einen deutlichen Nachteil bei IBR-Bek\u00e4mpfungsverfahren dar, die auf Markerimpfung beruhen, weshalb dieser Test im Allgemeinen nicht f\u00fcr die Untersuchung von Tankmilchproben empfohlen wird. Ziel dieser Studie war es, die Eignung eines kommerziell verf\u00fcgbaren Kits zur Anreicherung und Reinigung von Antik\u00f6rpern aus Milchproben zur Verbesserung der Sensitivit\u00e4t beim Nachweis IBR-gE-positiver K\u00fche aus Pool- und Tankmilchproben zu pr\u00fcfen.
Durch die Anreicherung von Poolmilchproben konnte die Sensitivit\u00e4t beim Nachweis einer IBR-gE-positiven Milch in Pools mit 49 negativen Milchproben von 5,4 % ohne Anreicherung auf 75,7 % nach Anreicherung erh\u00f6ht werden. Bei deutlich positiven und mittelgradig positiven Milchproben gelingt der Nachweis im Pool sicherer als bei schwach positiven Proben.
In einer Verlaufsstudie mit monatlicher Untersuchung von Tankmilchproben von drei IBR-gE-negativen, mit einem Marker-Impfstoff vakzinierten Herden sowie einer Herde mit
Schl\u00fcsselw\u00f6rter:<\/strong>
BHV-1, IBR-gE-Antik\u00f6rper, Poolmilch, Milchkonzentrierung <\/p>","primaryLanguage":"englisch","summary":"The low sensitivity of the IBR-gE ELISA compared to other diagnostic ELISA tests for IBR is a major disadvantage of IBR control programmes based on IBR marker vaccination. Therefore the IBR-gE ELISA is not generally recommended for testing pooled or bulk milk samples. The aim of this study was to determine the performance of a commercially available kit for concentrating and purifying antibodies in milk in order to improve the sensitivity of detecting IBR-gE antibody positive cows from pooled and bulk milk samples.
A single IBR-gE positive cow is likely to remain undetected in a pool of 49 negative milk samples without concentration. By contrast, the bulk milk concentration procedure improved sensitivity from 5.4% to 75.7% in a positive herd. Milk samples with a high or moderate positive signal are more likely to be detected after pool milk concentration compared to weak positive samples.
Whereas a follow up study involving a monthly testing of bulk milk samples from three marker vaccinated IBR-gE negative herds over a period of seven months yielded negative results each month, bulk milk from a herd containing","keywords":["BHV-1","IBR-gE antibody","pool milk","milk concentration"],"zusammenfassung":"Der IBR-gE-ELISA hat, im Vergleich mit anderen diagnostischen Tests zum Nachweis von IBR, eine geringe Sensitivit\u00e4t. Dies stellt einen deutlichen Nachteil bei IBR-Bek\u00e4mpfungsverfahren dar, die auf Markerimpfung beruhen, weshalb dieser Test im Allgemeinen nicht f\u00fcr die Untersuchung von Tankmilchproben empfohlen wird. Ziel dieser Studie war es, die Eignung eines kommerziell verf\u00fcgbaren Kits zur Anreicherung und Reinigung von Antik\u00f6rpern aus Milchproben zur Verbesserung der Sensitivit\u00e4t beim Nachweis IBR-gE-positiver K\u00fche aus Pool- und Tankmilchproben zu pr\u00fcfen.
Durch die Anreicherung von Poolmilchproben konnte die Sensitivit\u00e4t beim Nachweis einer IBR-gE-positiven Milch in Pools mit 49 negativen Milchproben von 5,4 % ohne Anreicherung auf 75,7 % nach Anreicherung erh\u00f6ht werden. Bei deutlich positiven und mittelgradig positiven Milchproben gelingt der Nachweis im Pool sicherer als bei schwach positiven Proben.
In einer Verlaufsstudie mit monatlicher Untersuchung von Tankmilchproben von drei IBR-gE-negativen, mit einem Marker-Impfstoff vakzinierten Herden sowie einer Herde mit","schluesselwoerter":["BHV-1","IBR-gE-Antik\u00f6rper","Poolmilch","Milchkonzentrierung"],"translatedTitle":"","abstractE":"The low sensitivity of the IBR-gE ELISA compared to other diagnostic ELISA tests for IBR is a major disadvantage of IBR control programmes based on IBR marker vaccination. Therefore the IBR-gE ELISA is not generally recommended for testing pooled or bulk milk samples. The aim of this study was to determine the performance of a commercially available kit for concentrating and purifying antibodies in milk in order to improve the sensitivity of detecting IBR-gE antibody positive cows from pooled and bulk milk samples. A single IBR-gE positive cow is likely to remain undetected in a pool of 49 negative milk samples without concentration. By contrast, the bulk milk concentration procedure improved sensitivity from 5.4% to 75.7% in a positive herd. Milk samples with a high or moderate positive signal are more likely to be detected after pool milk concentration compared to weak positive samples. Whereas a follow up study involving a monthly testing of bulk milk samples from three marker vaccinated IBR-gE negative herds over a period of seven months yielded negative results each month, bulk milk from a herd containing","date":{"year":2012,"date":"02\/2012","accepted":"2012-02-24"},"volume":"125","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"290-296","redirects":["bhv-1-ibr-ge-antibody-pool-milk-milk-concentration\/150\/3216\/68992","bhv-1-ibr-ge-antibody-pool-milk-milk-concentration\/150\/3130\/68992"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 02/2012 DO - 10.2376/0005-9366-125-290 LA - English N2 - The low sensitivity of the IBR-gE ELISA compared to other diagnostic ELISA tests for IBR is a major disadvantage of IBR control programmes based on IBR marker vaccination. Therefore the IBR-gE ELISA is not generally recommended for testing pooled or bulk milk samples. The aim of this study was to determine the performance of a commercially available kit for concentrating and purifying antibodies in milk in order to improve the sensitivity of detecting IBR-gE antibody positive cows from pooled and bulk milk samples. A single IBR-gE positive cow is likely to remain undetected in a pool of 49 negative milk samples without concentration. By contrast, the bulk milk concentration procedure improved sensitivity from 5.4% to 75.7% in a positive herd. Milk samples with a high or moderate positive signal are more likely to be detected after pool milk concentration compared to weak positive samples. Whereas a follow up study involving a monthly testing of bulk milk samples from three marker vaccinated IBR-gE negative herds over a period of seven months yielded negative results each month, bulk milk from a herd containing PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2012 SP - 290 EP - 296 T1 - Improving the sensitivity of the IBR-gE ELISA for testing IBR marker vaccinated cows from bulk milk T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Improving the sensitivity of the IBR-gE ELISA for testing IBR marker vaccinated cows from bulk milk VL - 125 SN - 0005-9366 ER -