TY - JOUR
KW - lymph node abscess
KW - goat
KW - Staphylococcus aureus
AU - O Szalus-Jordanow
AU - T Kanbar
AU - I Soedarmanto
AU - N Eissa
AU - J Alber
AU - C Lämmler
AU - M Zschöck
AU - R Weiss
AU - J Kaba
AU - T Frymus
AB - In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as  Staphylococcus aureus  subsp.  anaerobius . All 20 strains grew under microaerobic conditions, were negative ...
BT - Berliner und Münchener Tierärztliche Wochenschrift
C1 - {"oldId":68782,"title":"Phenotypic and genotypic properties of  Staphylococcus aureus  subsp.  anaerobius  isolated from lymph node abscesses of goats","teaserText":"In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as  Staphylococcus aureus  subsp.  anaerobius . All 20 strains grew under microaerobic conditions, were negative ...","content":"<p><span class=\"bold\">Summary<\/span><br \/>In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as <span class=\"bold\">Staphylococcus aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span>. All 20 strains grew under microaerobic conditions, were negative in the catalase test, showed the typical phenotypic properties of S. aureus and could genotypically be identified by a positive sa442, 23S rDNA, <span class=\"bold\">nuc, coa<\/span> and <span class=\"bold\">spa <\/span>PCR reaction. The variable regions of the <span class=\"bold\">coa<\/span> and <span class=\"bold\">spa<\/span> gene of the 20 strains appeared with uniform amplicon sizes, respectively. All 20 strains were negative for 12 additionally investigated enterotoxin encoding genes, tst and <span class=\"bold\">ssl7<\/span> and positive for the gene <span class=\"bold\">cap8<\/span>. Identical properties could be observed for S. aureus subsp. <span class=\"bold\">anaerobius<\/span> DSM 20714. Amplification and sequencing of kat gene of a single <span class=\"bold\">Staphylococcus aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span> strain of the present study and S. aureus subsp. <span class=\"bold\">anaerobius<\/span> DSM 20714 revealed a complete identity of the kat sequences of both strains and a <br \/>katB sequence obtained from GenBank (AJ000471). The bacteria were additionally investigated for relatedness by macrorestriction analysis of chromosomal DNA with subsequent pulsed-field gel electrophoresis (PFGE), yielding, corresponding to the above mentioned PCR results, identical PFGE patterns for all 20 <span class=\"bold\">Staphylococcus aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span> strains isolated in Western Poland and the <span class=\"bold\">S. aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span> reference strain DSM 20714. This indicates the clonal identity of the strains isolated in Western Poland and the S. aureus subsp. <span class=\"bold\">anaerobius<\/span> reference strain. The route of infection of the two herds in Western Poland with a bacterial clone originally isolated in Spain remains unclear. <br \/><br \/><span class=\"bold\">Keywords:<\/span><br \/>lymph node abscess, goat, <span class=\"bold\">Staphylococcus aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span>, pulsed-field gel electrophoresis <br \/><br \/><br \/><span class=\"bold\">Zusammenfassung<\/span><br \/>In den vorliegenden Untersuchungen wurden 20 Staphylokokken, isoliert von Lymphknotenabszessen von 19 Ziegen aus zwei Herden in West-Polen, als <span class=\"bold\">Staphylococcus aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span> identifiziert. Die 20 St\u00e4mme wuchsen unter mikroaeroben Bedingungen, waren Katalase-negativ, zeigten die typischen ph\u00e4notypischen Eigenschaften von S. aureus und konnten genotypisch durch eine positive sa442-, 23S rDNA-, <span class=\"bold\">nuc-, coa- <\/span>und <span class=\"bold\">spa<\/span>-PCR-Reaktion identifiziert werden. Die variable Region der Gene <span class=\"bold\">coa<\/span> und <span class=\"bold\">spa<\/span> zeigten dabei f\u00fcr alle 20 St\u00e4mme eine einheitliche Gr\u00f6\u00dfe. Die 20 St\u00e4mme waren negativ f\u00fcr 12 zus\u00e4tzlich untersuchte Enterotoxin-kodierende Gene, f\u00fcr tst und <span class=\"bold\">ssl7<\/span>, dagegen positiv f\u00fcr das Gen <span class=\"bold\">cap8<\/span>. Der Referenzstamm S. aureus subsp. <span class=\"bold\">anaerobius<\/span> DSM 20714 zeigte identische Eigenschaften. Die Amplifizierung und Sequenzierung des Katalase-kodierenden Gens kat von einem Stamm der vorliegenden Studie und von S. aureus subsp. <span class=\"bold\">anaerobius<\/span> DSM 20714 ergab eine vollst\u00e4ndige Identit\u00e4t der kat-Sequenzen beider St\u00e4mme und der <span class=\"bold\">katB<\/span>-Sequenz der GenBank. Die Bakterien wurden zus\u00e4tzlich durch Makrorestriktionsanalyse der chromosomalen DNA mittels Pulsfeldgelelektrophorese (PFGE) hinsichtlich ihres Verwandtschaftsgrades untersucht. Dies ergab, vergleichbar mit den bereits oben genannten PCR-Ergebnissen, vollst\u00e4ndig identische PFGE-Muster aller 20 St\u00e4mme, isoliert in West-Polen und dem Referenzstamm <span class=\"bold\">S. aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span> DSM 20714. Dies weist auf eine klonale Identit\u00e4t der St\u00e4mme aus West-Polen und des S. aureus subsp. <span class=\"bold\">anaerobius<\/span>- Referenzstammes hin, wobei \u00fcber den Infektionsweg von den St\u00e4mmen aus West-Polen und dem urspr\u00fcnglich in Spanien isolierten Referenzstamm keine Aussage getroffen werden kann. <br \/><br \/><span class=\"bold\">Schl\u00fcsselw\u00f6rter:<\/span><br \/>Lymphknotenabszess, Ziege, <span class=\"bold\">Staphylococcus aureus<\/span> subsp. <span class=\"bold\">anaerobius<\/span>, Pulsfeldgelelektrophorese <\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Mar 10, 2011 12:00:00 AM","toDate":"Dec 31, 2050 12:00:00 AM","oldUrls":["http:\/\/vetline.de\/lymph-node-abscess-goat-staphylococcus-aureus-subsp-anaerobius-pulsed-field-gel-electrophoresis\/150\/3130\/68782"],"doiLanguage":"englisch","doiProductFormat":"Online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr.","doiDocumentUri":"http:\/\/www.vetline.de\/lymph-node-abscess-goat-staphylococcus-aureus-subsp-anaerobius-pulsed-field-gel-electrophoresis\/150\/3130\/68782","doiSource":"Berl. M\u00fcnch. Tier\u00e4rztl. Wschr. 124: 3-4, 123-127 (2011)","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-124-123","doiFirstPage":"123","doiLastPage":"127","doiTransmitted":true,"doiAuthor":"Szalus-Jordanow O, Kanbar T, Soedarmanto I, Eissa N, Alber J, L\u00e4mmler C, Zsch\u00f6ck M, Weiss R, Kaba1 J, Frymus T","pdf":{"path":"http:\/\/data\/bmtw_2011_03_0123.pdf","title":"bmtw_2011_03_0123.pdf","description":"Phenotypic and genotypic properties of <i>Staphylococcus aureus<\/i> subsp. <i>anaerobius<\/i> isolated from lymph node abscesses of goats<br \/><br \/>"},"authors":[{"firstName":"O","middleName":"","lastName":"Szalus-Jordanow"},{"firstName":"T","middleName":"","lastName":"Kanbar"},{"firstName":"I","middleName":"","lastName":"Soedarmanto"},{"firstName":"N","middleName":"","lastName":"Eissa"},{"firstName":"J","middleName":"","lastName":"Alber"},{"firstName":"C","middleName":"","lastName":"L\u00e4mmler"},{"firstName":"M","middleName":"","lastName":"Zsch\u00f6ck"},{"firstName":"R","middleName":"","lastName":"Weiss"},{"firstName":"J","middleName":"","lastName":"Kaba1"},{"firstName":"T","middleName":"","lastName":"Frymus"}],"contentOptimised":"<p><strong>Summary<\/strong><br>In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong>. All 20 strains grew under microaerobic conditions, were negative in the catalase test, showed the typical phenotypic properties of S. aureus and could genotypically be identified by a positive sa442, 23S rDNA, <strong>nuc, coa<\/strong> and <strong>spa <\/strong>PCR reaction. The variable regions of the <strong>coa<\/strong> and <strong>spa<\/strong> gene of the 20 strains appeared with uniform amplicon sizes, respectively. All 20 strains were negative for 12 additionally investigated enterotoxin encoding genes, tst and <strong>ssl7<\/strong> and positive for the gene <strong>cap8<\/strong>. Identical properties could be observed for S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714. Amplification and sequencing of kat gene of a single <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong> strain of the present study and S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714 revealed a complete identity of the kat sequences of both strains and a <br>katB sequence obtained from GenBank (AJ000471). The bacteria were additionally investigated for relatedness by macrorestriction analysis of chromosomal DNA with subsequent pulsed-field gel electrophoresis (PFGE), yielding, corresponding to the above mentioned PCR results, identical PFGE patterns for all 20 <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong> strains isolated in Western Poland and the <strong>S. aureus<\/strong> subsp. <strong>anaerobius<\/strong> reference strain DSM 20714. This indicates the clonal identity of the strains isolated in Western Poland and the S. aureus subsp. <strong>anaerobius<\/strong> reference strain. The route of infection of the two herds in Western Poland with a bacterial clone originally isolated in Spain remains unclear. <br><br><strong>Keywords:<\/strong><br>lymph node abscess, goat, <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong>, pulsed-field gel electrophoresis <br><br><br><strong>Zusammenfassung<\/strong><br>In den vorliegenden Untersuchungen wurden 20 Staphylokokken, isoliert von Lymphknotenabszessen von 19 Ziegen aus zwei Herden in West-Polen, als <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong> identifiziert. Die 20 St\u00e4mme wuchsen unter mikroaeroben Bedingungen, waren Katalase-negativ, zeigten die typischen ph\u00e4notypischen Eigenschaften von S. aureus und konnten genotypisch durch eine positive sa442-, 23S rDNA-, <strong>nuc-, coa- <\/strong>und <strong>spa<\/strong>-PCR-Reaktion identifiziert werden. Die variable Region der Gene <strong>coa<\/strong> und <strong>spa<\/strong> zeigten dabei f\u00fcr alle 20 St\u00e4mme eine einheitliche Gr\u00f6\u00dfe. Die 20 St\u00e4mme waren negativ f\u00fcr 12 zus\u00e4tzlich untersuchte Enterotoxin-kodierende Gene, f\u00fcr tst und <strong>ssl7<\/strong>, dagegen positiv f\u00fcr das Gen <strong>cap8<\/strong>. Der Referenzstamm S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714 zeigte identische Eigenschaften. Die Amplifizierung und Sequenzierung des Katalase-kodierenden Gens kat von einem Stamm der vorliegenden Studie und von S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714 ergab eine vollst\u00e4ndige Identit\u00e4t der kat-Sequenzen beider St\u00e4mme und der <strong>katB<\/strong>-Sequenz der GenBank. Die Bakterien wurden zus\u00e4tzlich durch Makrorestriktionsanalyse der chromosomalen DNA mittels Pulsfeldgelelektrophorese (PFGE) hinsichtlich ihres Verwandtschaftsgrades untersucht. Dies ergab, vergleichbar mit den bereits oben genannten PCR-Ergebnissen, vollst\u00e4ndig identische PFGE-Muster aller 20 St\u00e4mme, isoliert in West-Polen und dem Referenzstamm <strong>S. aureus<\/strong> subsp. <strong>anaerobius<\/strong> DSM 20714. Dies weist auf eine klonale Identit\u00e4t der St\u00e4mme aus West-Polen und des S. aureus subsp. <strong>anaerobius<\/strong>- Referenzstammes hin, wobei \u00fcber den Infektionsweg von den St\u00e4mmen aus West-Polen und dem urspr\u00fcnglich in Spanien isolierten Referenzstamm keine Aussage getroffen werden kann. <br><br><strong>Schl\u00fcsselw\u00f6rter:<\/strong><br>Lymphknotenabszess, Ziege, <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong>, Pulsfeldgelelektrophorese <\/p>","primaryLanguage":"englisch","summary":"In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong>. All 20 strains grew under microaerobic conditions, were negative in the catalase test, showed the typical phenotypic properties of S. aureus and could genotypically be identified by a positive sa442, 23S rDNA, <strong>nuc, coa<\/strong> and <strong>spa <\/strong>PCR reaction. The variable regions of the <strong>coa<\/strong> and <strong>spa<\/strong> gene of the 20 strains appeared with uniform amplicon sizes, respectively. All 20 strains were negative for 12 additionally investigated enterotoxin encoding genes, tst and <strong>ssl7<\/strong> and positive for the gene <strong>cap8<\/strong>. Identical properties could be observed for S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714. Amplification and sequencing of kat gene of a single <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong> strain of the present study and S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714 revealed a complete identity of the kat sequences of both strains and a <br>katB sequence obtained from GenBank (AJ000471). The bacteria were additionally investigated for relatedness by macrorestriction analysis of chromosomal DNA with subsequent pulsed-field gel electrophoresis (PFGE), yielding, corresponding to the above mentioned PCR results, identical PFGE patterns for all 20 <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong> strains isolated in Western Poland and the <strong>S. aureus<\/strong> subsp. <strong>anaerobius<\/strong> reference strain DSM 20714. This indicates the clonal identity of the strains isolated in Western Poland and the S. aureus subsp. <strong>anaerobius<\/strong> reference strain. The route of infection of the two herds in Western Poland with a bacterial clone originally isolated in Spain remains unclear.","keywords":["lymph node abscess","goat","Staphylococcus aureus"],"zusammenfassung":"In den vorliegenden Untersuchungen wurden 20 Staphylokokken, isoliert von Lymphknotenabszessen von 19 Ziegen aus zwei Herden in West-Polen, als <strong>Staphylococcus aureus<\/strong> subsp. <strong>anaerobius<\/strong> identifiziert. Die 20 St\u00e4mme wuchsen unter mikroaeroben Bedingungen, waren Katalase-negativ, zeigten die typischen ph\u00e4notypischen Eigenschaften von S. aureus und konnten genotypisch durch eine positive sa442-, 23S rDNA-, <strong>nuc-, coa- <\/strong>und <strong>spa<\/strong>-PCR-Reaktion identifiziert werden. Die variable Region der Gene <strong>coa<\/strong> und <strong>spa<\/strong> zeigten dabei f\u00fcr alle 20 St\u00e4mme eine einheitliche Gr\u00f6\u00dfe. Die 20 St\u00e4mme waren negativ f\u00fcr 12 zus\u00e4tzlich untersuchte Enterotoxin-kodierende Gene, f\u00fcr tst und <strong>ssl7<\/strong>, dagegen positiv f\u00fcr das Gen <strong>cap8<\/strong>. Der Referenzstamm S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714 zeigte identische Eigenschaften. Die Amplifizierung und Sequenzierung des Katalase-kodierenden Gens kat von einem Stamm der vorliegenden Studie und von S. aureus subsp. <strong>anaerobius<\/strong> DSM 20714 ergab eine vollst\u00e4ndige Identit\u00e4t der kat-Sequenzen beider St\u00e4mme und der <strong>katB<\/strong>-Sequenz der GenBank. Die Bakterien wurden zus\u00e4tzlich durch Makrorestriktionsanalyse der chromosomalen DNA mittels Pulsfeldgelelektrophorese (PFGE) hinsichtlich ihres Verwandtschaftsgrades untersucht. Dies ergab, vergleichbar mit den bereits oben genannten PCR-Ergebnissen, vollst\u00e4ndig identische PFGE-Muster aller 20 St\u00e4mme, isoliert in West-Polen und dem Referenzstamm <strong>S. aureus<\/strong> subsp. <strong>anaerobius<\/strong> DSM 20714. Dies weist auf eine klonale Identit\u00e4t der St\u00e4mme aus West-Polen und des S. aureus subsp. <strong>anaerobius<\/strong>- Referenzstammes hin, wobei \u00fcber den Infektionsweg von den St\u00e4mmen aus West-Polen und dem urspr\u00fcnglich in Spanien isolierten Referenzstamm keine Aussage getroffen werden kann.","schluesselwoerter":["Lymphknotenabszess","Ziege","Staphylococcus aureus"],"translatedTitle":"","abstractE":"In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as  Staphylococcus aureus  subsp.  anaerobius . All 20 strains grew under microaerobic conditions, were negative ...","date":{"year":2011,"date":"03\/2011","accepted":"2011-03-10"},"volume":"124","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"123-127","redirects":["lymph-node-abscess-goat-staphylococcus-aureus-subsp-anaerobius-pulsed-field-gel-electrophoresis\/150\/3130\/68782"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]}
CY - Hannover
DA - 03/2011
DO - 10.2376/0005-9366-124-123
LA - English
N2 - In the present study 20 staphylococci isolated from lymph node abscesses of 19 goats of two herds in Western Poland could be identified as  Staphylococcus aureus  subsp.  anaerobius . All 20 strains grew under microaerobic conditions, were negative ...
PB - Schlütersche Verlagsgesellschaft mbH & Co. KG
PP - Hannover
PY - 2011
SP - 123
EP - 127
T1 - Phenotypic and genotypic properties of  Staphylococcus aureus  subsp.  anaerobius  isolated from lymph node abscesses of goats
T2 - Berliner und Münchener Tierärztliche Wochenschrift
TI - Phenotypic and genotypic properties of  Staphylococcus aureus  subsp.  anaerobius  isolated from lymph node abscesses of goats
VL - 124
SN - 0005-9366
ER -