TY - JOUR KW - Campylobacter jejuni KW - microbial community KW - PCR-SSCP KW - sequencing KW - chicken AU - D Sofka AU - A Pfeifer AU - B Gleiß AU - P Paulsen AU - F Hilbert AB - In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter’s interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of C. jejuni and C. coli, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci, Staphylococci, Enterobacteriaceae, E. coli, and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by C. jejuni. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter colonization and disease. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":84717,"title":"Changes within the intestinal flora of broilers by colonisation with Campylobacter jejuni","topline":"Open Access | CARO-Themenheft","teaserText":"Ver\u00e4nderungen der Darmflora des Gefl\u00fcgels durch Besiedelung mit Campylobacter jejuni","content":"

Summary<\/span><\/p>

In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter<\/span> colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter\u2019s interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of C. jejuni<\/span> and C. coli<\/span>, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci,<\/span> Staphylococci<\/span>, Enterobacteriaceae<\/span>, E. coli,<\/span> and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter <\/span>spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by C. jejuni<\/span>. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni<\/span>, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter<\/span> colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni<\/span> is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter<\/span> colonization and disease. <\/p>

Keywords<\/span><\/p>

Campylobacter jejuni<\/span>, microbial community, PCR-SSCP, sequencing, chicken<\/p>

Zusammenfassung Die Campylobacteriose stellt europaweit die h\u00e4ufigste lebensmittelbedingte bakterielle Infektionskrankheit dar. Als Hauptursache dieser Infektion gilt nach wie vor Gefl\u00fcgelfleisch. Da alle bisherigen Bem\u00fchungen, eine Besiedelung von Gefl\u00fcgelherden mit Campylobacter<\/span> zu verhindern oder humane Infektionsraten zu senken, fehlschlugen, gilt es neue Erkenntnisse zu gewinnen. In der vorliegenden Arbeit wurden f\u00e4kale und z\u00e4kale Proben von \u00f6sterreichischem Mastgefl\u00fcgel auf das Vorhandensein von C. jejuni<\/span> und C. coli <\/span>untersucht und die intestinale Mikrobiota mit genetischen Methoden (PCR-SSCP) und kulturellen Nachweisen von Laktobazillen, Enterokokken, Staphylokokken, Enterobacteriaceae<\/span>, E. coli<\/span>, ebenso wie die Ermittlung der aeroben Gesamtkeimzahl, analysiert. Weiters sind zehn z\u00e4kale Proben, die w\u00e4hrend einer stattfindenden Herdendurchseuchung entnommen wurden, mittels einer \u201ehigh throughput sequencing\u201d Analyse untersucht worden. Mit allen drei Methoden konnte eine \u00c4nderung der mikrobiellen Flora durch eine Besiedelung mit Campylobacter<\/span> nachgewiesen werden. In Campylobacter<\/span> positiven Proben wurden signifikant weniger Mikroorganismen detektiert. Dies ist m\u00f6glicherweise durch einen h\u00f6heren Wassergehalt in den f\u00e4kalen Proben zu erkl\u00e4ren, was auf die Vorstufe einer Diarrh\u00f6 hinweisen k\u00f6nnte. Mittels PCR-SSCP Analyse wurde eine Ver\u00e4nderung in der Zusammensetzung der Mikrobiota nachgewiesen, was auch mittels dem \u201ehigh throughput sequencing\u201d erkennbar war. Dieser Unterschied war nur als Tendenz erkennbar und nicht statistisch signifikant. Zusammenfassend konnte mit dieser Studie best\u00e4tigt werden, dass C. jejuni <\/span> mit der intestinalen Mikroflora interagiert. Diese Erkenntnis sollte in die Planung neuer Bek\u00e4mpfungsstrategien miteinbezogen werden.<\/p>

Schl\u00fcsselw\u00f6rter<\/span><\/p>

Campylobacter jejuni<\/span>, mikrobielle Flora, PCR-SSCP, Sequenzierung, Huhn<\/p>","categories":["Open Access","Abostufe frei"],"fromDate":"Jan 16, 2015 9:43:21 AM","oldUrls":["http:\/\/vetline.de\/changes-within-the-intestinal-flora-of-broilers-by-colonisation-with-campylobacter-jejuni\/150\/3216\/84717"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr","doiDocumentUri":"http:\/\/vetline.de\/changes-within-the-intestinal-flora-of-broilers-by-colonisation-with-campylobacter-jejuni\/150\/3216\/84717\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 104\u2013110","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-104","doiFirstPage":"104","doiLastPage":"110","doiTransmitted":true,"doiAuthor":"Sofka D, Pfeifer A, Glei\u00df B, Paulsen P, Hilbert F","pdf":{"path":"http:\/\/data\/BMW_2015_0104.pdf","title":"BMW_2015_0104.pdf","description":"Changes within the intestinal flora of broilers by colonisation with Campylobacter jejuni"},"authors":[{"firstName":"D","middleName":"","lastName":"Sofka"},{"firstName":"A","middleName":"","lastName":"Pfeifer"},{"firstName":"B","middleName":"","lastName":"Glei\u00df"},{"firstName":"P","middleName":"","lastName":"Paulsen"},{"firstName":"F","middleName":"","lastName":"Hilbert"}],"contentOptimised":"

Summary<\/strong><\/p>

In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter<\/em> colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter\u2019s interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of C. jejuni<\/em> and C. coli<\/em>, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci,<\/em> Staphylococci<\/em>, Enterobacteriaceae<\/em>, E. coli,<\/em> and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter <\/em>spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by C. jejuni<\/em>. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni<\/em>, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter<\/em> colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni<\/em> is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter<\/em> colonization and disease. <\/p>

Keywords:<\/strong><\/p>

Campylobacter jejuni<\/em>, microbial community, PCR-SSCP, sequencing, chicken<\/p>

Zusammenfassung Die Campylobacteriose stellt europaweit die h\u00e4ufigste lebensmittelbedingte bakterielle Infektionskrankheit dar. Als Hauptursache dieser Infektion gilt nach wie vor Gefl\u00fcgelfleisch. Da alle bisherigen Bem\u00fchungen, eine Besiedelung von Gefl\u00fcgelherden mit Campylobacter<\/em> zu verhindern oder humane Infektionsraten zu senken, fehlschlugen, gilt es neue Erkenntnisse zu gewinnen. In der vorliegenden Arbeit wurden f\u00e4kale und z\u00e4kale Proben von \u00f6sterreichischem Mastgefl\u00fcgel auf das Vorhandensein von C. jejuni<\/em> und C. coli <\/em>untersucht und die intestinale Mikrobiota mit genetischen Methoden (PCR-SSCP) und kulturellen Nachweisen von Laktobazillen, Enterokokken, Staphylokokken, Enterobacteriaceae<\/em>, E. coli<\/em>, ebenso wie die Ermittlung der aeroben Gesamtkeimzahl, analysiert. Weiters sind zehn z\u00e4kale Proben, die w\u00e4hrend einer stattfindenden Herdendurchseuchung entnommen wurden, mittels einer \u201ehigh throughput sequencing\u201d Analyse untersucht worden. Mit allen drei Methoden konnte eine \u00c4nderung der mikrobiellen Flora durch eine Besiedelung mit Campylobacter<\/em> nachgewiesen werden. In Campylobacter<\/em> positiven Proben wurden signifikant weniger Mikroorganismen detektiert. Dies ist m\u00f6glicherweise durch einen h\u00f6heren Wassergehalt in den f\u00e4kalen Proben zu erkl\u00e4ren, was auf die Vorstufe einer Diarrh\u00f6 hinweisen k\u00f6nnte. Mittels PCR-SSCP Analyse wurde eine Ver\u00e4nderung in der Zusammensetzung der Mikrobiota nachgewiesen, was auch mittels dem \u201ehigh throughput sequencing\u201d erkennbar war. Dieser Unterschied war nur als Tendenz erkennbar und nicht statistisch signifikant. Zusammenfassend konnte mit dieser Studie best\u00e4tigt werden, dass C. jejuni <\/em> mit der intestinalen Mikroflora interagiert. Diese Erkenntnis sollte in die Planung neuer Bek\u00e4mpfungsstrategien miteinbezogen werden.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong><\/p>

Campylobacter jejuni<\/em>, mikrobielle Flora, PCR-SSCP, Sequenzierung, Huhn<\/p>","primaryLanguage":"englisch","zusammenfassung":"

Die Campylobacteriose stellt europaweit die h\u00e4ufigste lebensmittelbedingte bakterielle Infektionskrankheit dar. Als Hauptursache dieser Infektion gilt nach wie vor Gefl\u00fcgelfleisch. Da alle bisherigen Bem\u00fchungen, eine Besiedelung von Gefl\u00fcgelherden mit Campylobacter<\/em> zu verhindern oder humane Infektionsraten zu senken, fehlschlugen, gilt es neue Erkenntnisse zu gewinnen. In der vorliegenden Arbeit wurden f\u00e4kale und z\u00e4kale Proben von \u00f6sterreichischem Mastgefl\u00fcgel auf das Vorhandensein von C. jejuni<\/em> und C. coli <\/em>untersucht und die intestinale Mikrobiota mit genetischen Methoden (PCR-SSCP) und kulturellen Nachweisen von Laktobazillen, Enterokokken, Staphylokokken, Enterobacteriaceae<\/em>, E. coli<\/em>, ebenso wie die Ermittlung der aeroben Gesamtkeimzahl, analysiert. Weiters sind zehn z\u00e4kale Proben, die w\u00e4hrend einer stattfindenden Herdendurchseuchung entnommen wurden, mittels einer \u201ehigh throughput sequencing\u201d Analyse untersucht worden. Mit allen drei Methoden konnte eine \u00c4nderung der mikrobiellen Flora durch eine Besiedelung mit Campylobacter<\/em> nachgewiesen werden. In Campylobacter<\/em> positiven Proben wurden signifikant weniger Mikroorganismen detektiert. Dies ist m\u00f6glicherweise durch einen h\u00f6heren Wassergehalt in den f\u00e4kalen Proben zu erkl\u00e4ren, was auf die Vorstufe einer Diarrh\u00f6 hinweisen k\u00f6nnte. Mittels PCR-SSCP Analyse wurde eine Ver\u00e4nderung in der Zusammensetzung der Mikrobiota nachgewiesen, was auch mittels dem \u201ehigh throughput sequencing\u201d erkennbar war. Dieser Unterschied war nur als Tendenz erkennbar und nicht statistisch signifikant. Zusammenfassend konnte mit dieser Studie best\u00e4tigt werden, dass C. jejuni <\/em> mit der intestinalen Mikroflora interagiert. Diese Erkenntnis sollte in die Planung neuer Bek\u00e4mpfungsstrategien miteinbezogen werden.<\/p>","summary":"<\/p>

In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter<\/em> colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter\u2019s interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of C. jejuni<\/em> and C. coli<\/em>, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci,<\/em> Staphylococci<\/em>, Enterobacteriaceae<\/em>, E. coli,<\/em> and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter <\/em>spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by C. jejuni<\/em>. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni<\/em>, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter<\/em> colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni<\/em> is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter<\/em> colonization and disease. <\/p>

","keywords":["Campylobacter jejuni","microbial community","PCR-SSCP","sequencing","chicken"],"schluesselwoerter":["Campylobacter jejuni","mikrobielle Flora","PCR-SSCP","Sequenzierung","Huhn"],"translatedTitle":"Ver\u00e4nderungen der Darmflora des Gefl\u00fcgels durch Besiedelung mit Campylobacter jejuni","abstractE":" In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter\u2019s interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of C. jejuni and C. coli, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci, Staphylococci, Enterobacteriaceae, E. coli, and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by C. jejuni. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter colonization and disease. ","date":{"year":2015,"date":"01\/2015","accepted":"2015-01-16"},"volume":"128","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"104-110","redirects":["changes-within-the-intestinal-flora-of-broilers-by-colonisation-with-campylobacter-jejuni\/150\/3216\/84717"],"tierartCategories":[],"artikelartCategories":["Open Access","Abostufe frei"]} CY - Hannover DA - 01/2015 DO - 10.2376/0005-9366-128-104 LA - English N2 - In most European countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. Chicken meat is considered the main source of infection. Since most strategies assessed so far, in reducing Campylobacter colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding Campylobacter’s interaction with the host is needed. In this study we analysed fecal and cecal samples of five chicken flocks of different Austrian farms for the occurrence of C. jejuni and C. coli, and analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid bacteria, Enterococci, Staphylococci, Enterobacteriaceae, E. coli, and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock during colonization with Campylobacter spp. was analysed by high throughput sequencing. With all three methods used we could detect a change within the microbiota caused by C. jejuni. Enumeration of different bacteria was significantly lower in fecal samples positive for C. jejuni, pointing out that a higher water content and thus, a preliminary stage of diarrhea might appear during Campylobacter colonization. By PCR-SSCP analysis the microbiota composition differed between colonized and non-colonized chicken fecal samples. This could also be detected in community analysis by high throughput sequencing, but this difference was only a tendency and not statistically significant. It can be concluded that C. jejuni is interacting with the intestinal microflora in their respective hosts and hence, this has to be taken into account when providing new strategies to combat Campylobacter colonization and disease. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2015 SP - 104 EP - 110 T1 - Changes within the intestinal flora of broilers by colonisation with Campylobacter jejuni T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Changes within the intestinal flora of broilers by colonisation with Campylobacter jejuni TT - Veränderungen der Darmflora des Geflügels durch Besiedelung mit Campylobacter jejuni VL - 128 SN - 0005-9366 ER -