TY - JOUR KW - PRRSV KW - wild boar KW - ORF7 KW - genetic typing AU - S Vilček AU - L Molnar AU - M Vlasakova AU - A Jacková AB - PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence similarity to Lelystad reference strain and Porcilis® PRRS vaccine strain, respectively. The origin of PRRSV in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":84499,"title":"The first detection of PRRSV in wild boars in Slovakia","topline":"","teaserText":"Der erste Nachweis von PRRSV bei Wildschweinen in der Slowakischen Republik","content":"

Summary<\/span>
PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence similarity to Lelystad reference strain and Porcilis\u00ae PRRS vaccine strain, respectively. The origin of PRRSV in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars.<\/p>

Keywords<\/span>
PRRSV, wild boar, ORF7, genetic typing<\/p>

Zusammenfassung<\/span>
Bei zwei von 129 Wildschweinen aus dem Osten der Slowakischen Republik wurde PRRSV mittels nested RT-PCR nachgewiesen. Die Virusisolate wurden genetisch typisiert und zeigten 99,7 % (Probe 14WB) bzw. 100 % (Probe 10WB) Identit\u00e4t in der Nukleotidsequenz der ORF 7 zum Lelystad Referenzstamm und zum Porcilis\u00ae PRRS Impfstamm. Die Herkunft von PRRSV bei diesen Wildschweinen ist nicht gekl\u00e4rt, aber es ist sehr wahrscheinlich, dass das Virus von geimpften Hausschweinen auf die Wildschweine \u00fcbertragen wurde.<\/p>

Schl\u00fcsselworte<\/span>
PRRSV, Wildschwein, ORF 7, Genotypisierung<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jan 8, 2015 11:00:00 PM","oldUrls":["http:\/\/vetline.de\/the-first-detection-of-prrsv-in-wild-boars-in-slovakia\/150\/3130\/84499"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/the-first-detection-of-prrsv-in-wild-boars-in-slovakia\/150\/3130\/84499\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 1\u2013 (2015) ","doiissn":"0005-966","doiNr":"10.23276\/0005-966-128-31","doiFirstPage":"31","doiLastPage":"33","doiTransmitted":false,"doiAuthor":"Vilcek S, Molnar L, Vlasakova M, Jackova A","pdf":{"path":"http:\/\/data\/BMW_2015_01_02_0031.pdf","title":"BMW_2015_01_02_0031.pdf","description":"The first detection of PRRSV in wild boars in Slovakia"},"authors":[{"firstName":"S","middleName":"","lastName":"Vilcek"},{"firstName":"L","middleName":"","lastName":"Molnar"},{"firstName":"M","middleName":"","lastName":"Vlasakova"},{"firstName":"A","middleName":"","lastName":"Jackova"}],"contentOptimised":"

Summary<\/strong>
PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence similarity to Lelystad reference strain and Porcilis\u00ae PRRS vaccine strain, respectively. The origin of PRRSV in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars.<\/p>

Keywords:<\/strong>
PRRSV, wild boar, ORF7, genetic typing<\/p>

Zusammenfassung<\/strong>
Bei zwei von 129 Wildschweinen aus dem Osten der Slowakischen Republik wurde PRRSV mittels nested RT-PCR nachgewiesen. Die Virusisolate wurden genetisch typisiert und zeigten 99,7 % (Probe 14WB) bzw. 100 % (Probe 10WB) Identit\u00e4t in der Nukleotidsequenz der ORF 7 zum Lelystad Referenzstamm und zum Porcilis\u00ae PRRS Impfstamm. Die Herkunft von PRRSV bei diesen Wildschweinen ist nicht gekl\u00e4rt, aber es ist sehr wahrscheinlich, dass das Virus von geimpften Hausschweinen auf die Wildschweine \u00fcbertragen wurde.<\/p>

Schl\u00fcsselworte<\/strong>
PRRSV, Wildschwein, ORF 7, Genotypisierung<\/p>","primaryLanguage":"englisch","summary":"PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence similarity to Lelystad reference strain and Porcilis\u00ae PRRS vaccine strain, respectively. The origin of PRRSV in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars.<\/p>

","keywords":["PRRSV","wild boar","ORF7","genetic typing"],"zusammenfassung":"Bei zwei von 129 Wildschweinen aus dem Osten der Slowakischen Republik wurde PRRSV mittels nested RT-PCR nachgewiesen. Die Virusisolate wurden genetisch typisiert und zeigten 99,7 % (Probe 14WB) bzw. 100 % (Probe 10WB) Identit\u00e4t in der Nukleotidsequenz der ORF 7 zum Lelystad Referenzstamm und zum Porcilis\u00ae PRRS Impfstamm. Die Herkunft von PRRSV bei diesen Wildschweinen ist nicht gekl\u00e4rt, aber es ist sehr wahrscheinlich, dass das Virus von geimpften Hausschweinen auf die Wildschweine \u00fcbertragen wurde.<\/p>

Schl\u00fcsselworte<\/strong>
PRRSV, Wildschwein, ORF 7, Genotypisierung<\/p>","translatedTitle":"Der erste Nachweis von PRRSV bei Wildschweinen in der Slowakischen Republik","abstractE":"PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence similarity to Lelystad reference strain and Porcilis\u00ae PRRS vaccine strain, respectively. The origin of PRRSV in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars.","date":{"year":2015,"date":"01\/2015","accepted":"2015-01-08"},"volume":"128","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"31-33","redirects":["the-first-detection-of-prrsv-in-wild-boars-in-slovakia\/150\/3130\/84499"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 01/2015 DO - 10.2376/0005-966-128-31 ET - 1/2 LA - English N2 - PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence similarity to Lelystad reference strain and Porcilis® PRRS vaccine strain, respectively. The origin of PRRSV in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2015 SP - 31 EP - 33 T1 - The first detection of PRRSV in wild boars in Slovakia T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - The first detection of PRRSV in wild boars in Slovakia TT - Der erste Nachweis von PRRSV bei Wildschweinen in der Slowakischen Republik VL - 128 SN - 0005-966 ER -