TY - JOUR KW - Campylobacter spp. KW - C. coli KW - probiotics KW - Enterococcus faecium KW - pig AU - K Bratz AU - G Gölz AU - P Janczyk AU - K Nöckler AU - T Alter AB - Campylobacter (C.) spp. are well recognised as the leading cause of bacterial foodborne diarrheal disease worldwide, with C. jejuni and C. coli as the most important species. C. coli is highly abundant in pigs and pork meat has often been implicated as a source for human infection. Intestinal colonisation of C. coli in pigs plays a role in carcass contamination during slaughter. Different pre-harvest intervention measures are proposed to reduce the C. coli burden in the porcine intestine. Among others, the use of probiotics to prevent or reduce the colonisation of intestinal pathogens is discussed. One aim of this study was to screen a variety of probiotics to evaluate their inhibitory activity against Campylobacter spp. in vitro. Therefore, cell-free culture supernatants of Lactobacillus spp., Bifidobacterium spp., Enterococcus (E.) faecium NCIMB 10415, and Escherichia coli Nissle 1917 were tested against C. jejuni and C. coli by a well-diffusion agar assay. Seven out of eleven Lactobacillus strains showed an inhibitory activity against at least one of the three tested Campylobacter strains. This antagonistic activity against Campylobacter spp. was caused by the production of organic acids that lowered the pH. Application with pH neutralised cell-free culture supernatants abolished this inhibitory effect. Other tested strains with probiotic properties showed no inhibitory activity against any Campylobacter spp. strain. The strain E. faecium NCIMB 10415 was chosen to test its inhibitory activity against C. coli in vivo. Twenty weaned piglets were allocated into two groups, a probiotic group and a control group. The diet of the probiotic group was supplemented with E. faecium NCIMB 10415 (109 cfu/kg feed, Cylactin) since weaning, whereas the control group received no probiotic treatment. All piglets were naturally colonised with C. coli. The excretion load of C. coli was monitored for 28 days. The results indicate that dietary supplementation of E. faecium NCIMB 10415 did not significantly affect C. coli excretion levels in pigs. In this study, E. faecium NCIMB 10415 showed no antagonistic activity against C. coli in vitro and in vivo and had no impact on the growth performance of weaned piglets. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":84333,"title":"Analysis of in vitro and in vivo effects of probiotics against Campylobacter spp.","topline":"Open Access | CARO-Themenheft","teaserText":"Analyse der In-vitro- und In-vivo-Effekte von Probiotika gegen Campylobacter spp.","content":"

Summary<\/span><\/p>

Campylobacter (C.)<\/span> spp. are well recognised as the leading cause of bacterial foodborne diarrheal disease worldwide, with C. jejuni <\/span>and C. coli <\/span>as the most important species. C. col<\/span>i is highly abundant in pigs and pork meat has often been implicated as a source for human infection. Intestinal colonisation of C. coli<\/span> in pigs plays a role in carcass contamination during slaughter. Different pre-harvest intervention measures are proposed to reduce the C. coli<\/span> burden in the porcine intestine. Among others, the use of probiotics to prevent or reduce the colonisation of intestinal pathogens is discussed. One aim of this study was to screen a variety of probiotics to evaluate their inhibitory activity against Campylobacter <\/span>spp. in vitro. Therefore, cell-free culture supernatants of Lactobacillus<\/span> spp., Bifidobacterium<\/span> spp., Enterococcus (E.) faecium<\/span> NCIMB 10415, and Escherichia coli<\/span> Nissle 1917 were tested against C<\/span>. jejuni <\/span>and C. coli<\/span> by a well-diffusion agar assay. Seven out of eleven Lactobacillus <\/span>strains showed an inhibitory activity against at least one of the three tested Campylobacter <\/span>strains. This antagonistic activity against Campylobacter<\/span> spp. was caused by the production of organic acids that lowered the pH. Application with pH neutralised cell-free culture supernatants abolished this inhibitory effect. Other tested strains with probiotic properties showed no inhibitory activity against any Campylobacter <\/span>spp. strain. The strain E. faecium <\/span>NCIMB 10415 was chosen to test its inhibitory activity against C. coli <\/span>in vivo. Twenty weaned piglets were allocated into two groups, a probiotic group and a control group. The diet of the probiotic group was supplemented with E. faecium<\/span> NCIMB 10415 (109 cfu\/kg feed, Cylactin) since weaning, whereas the control group received no probiotic treatment. All piglets were naturally colonised with C. coli.<\/span> The excretion load of C. coli <\/span>was monitored for 28 days. The results indicate that dietary supplementation of E. faecium <\/span>NCIMB 10415 did not significantly affect C. coli<\/span> excretion levels in pigs. In this study, E. faecium<\/span> NCIMB 10415 showed no antagonistic activity against C. coli <\/span> in vitro and in vivo and had no impact on the growth performance of weaned piglets.<\/p>

Keywords<\/span><\/p>

Campylobacter<\/span> spp., C. coli<\/span>, probiotics, Enterococcus faecium<\/span>, pig<\/p>

Zusammenfassung<\/span><\/p>

Campylobacter (C.) <\/span>spp. sind eine der h\u00e4ufigsten Ursachen f\u00fcr bakterielle lebensmittelassoziierte Infektionen weltweit. Hierbei stellen die Spezies C. jejuni<\/span> und C. coli<\/span> die wichtigsten Vertreter dar. C. coli <\/span>dominiert im Schwein, daher wird Schweinefleisch oft als Hauptquelle f\u00fcr C. coli-<\/span>Infektionen beim Menschen angesehen. Die Besiedlung des Darms mit C. coli <\/span>beim Schwein spielt bei der Kontamination des Schweinefleisches w\u00e4hrend der Schlachtung eine entscheidende Rolle. Der Einsatz verschiedener sogenannter Pre-harvest-Interventionsma\u00dfnahmen zur Reduktion der C. coli-<\/span>Belastung im Schwein wird diskutiert. Unter anderem wird die Anwendung probiotischer Bakterien zur Vermeidung oder Reduktion der Besiedlung intestinaler Pathogene im Schwein derzeit in Betracht gezogen. Ein Ziel dieser Studie war es, verschiedene Probiotika-St\u00e4mme auf ihre hemmende Wirkung gegen\u00fcber Campylobacter<\/span> spp. in vitro zu testen. Daf\u00fcr wurde die inhibierende Wirkung zellfreier Kultur-\u00dcberst\u00e4nde von Lactobacillus<\/span> spp. Bifidobacterium <\/span> spp., Enterococcus (E.) faecium <\/span>NCIMB 10415 sowie Escherichia coli<\/span> Nissle 1917 gegen\u00fcber C.<\/span> jejuni<\/span> und C. coli<\/span> mittels Agar-Diffusionstest \u00fcberpr\u00fcft. Bei sieben von elf Lactobacillus<\/span>-St\u00e4mmen konnte eine inhibierende Wirkung gegen mindestens einen der drei getesteten Campylobacter<\/span>-St\u00e4mme nachgewiesen werden. Dieser antagonistische Effekt kann auf die Produktion von organischen S\u00e4uren und dem damit verminderten pH-Wert zur\u00fcckgef\u00fchrt werden. Der Einsatz eines pH neutralisierten zellfreien Kultur-\u00dcberstandes hob diesen inhibierenden Effekt auf. Andere getestete St\u00e4mme mit probiotischen Eigenschaften zeigten hingegen keine inhibierende Wirkung gegen\u00fcber Campylobacter<\/span> spp. E. faecium<\/span> NCIMB 10415 wurde zur Testung seiner inhibierenden Wirkung gegen\u00fcber C. coli <\/span>f\u00fcr den Einsatz im Tierversuch ausgew\u00e4hlt. Daf\u00fcr wurden 20 abgesetzte Ferkel auf zwei Gruppen aufgeteilt, einer probiotischen Gruppe und einer Kontrollgruppe. Der probiotischen Gruppe wurde bereits w\u00e4hrend der Saugphase das Probiotikum E. faecium<\/span> NCIMB 10415 (109 cfu\/kg Futter, Cylactin) im Futter angeboten, wohingegen bei der Kontrollgruppe keine probiotische Zugabe erfolgte. Alle Ferkel waren auf nat\u00fcrliche Weise mit C. coli<\/span> besiedelt. Die Ausscheidung von C. coli<\/span> wurde f\u00fcr 28 Tage kontrolliert. Die Ergebnisse zeigen, dass die Verf\u00fctterung von E. faecium<\/span> NCIMB 10415 keinen signifikanten Einfluss auf die ausgeschiedene C. coli-<\/span>Zellzahl der Ferkel hat. In dieser Studie konnte keine antagonistische Wirkung von E. faecium<\/span> NCIMB 10415 gegen\u00fcber C. coli<\/span> in vitro und in vivo gezeigt werden. Die Wachstumsleistung entw\u00f6hnter Ferkel konnte durch das Probiotikum nicht gesteigert werden.<\/p>

Schl\u00fcsselw\u00f6rter<\/span><\/p>

Campylobacter <\/span>spp., Campylobacter coli<\/span>, Probiotikum, Enterococcus faecium<\/span>, Schwein<\/p>","categories":["Open Access","Abostufe frei"],"fromDate":"Dec 19, 2014 10:15:32 AM","oldUrls":["http:\/\/vetline.de\/analysis-of-in-vitro-and-in-vivo-effects-of-probiotics-against-campylobacter-spp\/150\/3216\/84333"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr","doiDocumentUri":"http:\/\/vetline.de\/analysis-of-in-vitro-and-in-vivo-effects-of-probiotics-against-campylobacter-spp\/150\/3216\/84333\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 155\u2013162","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-155","doiFirstPage":"155","doiLastPage":"162","doiTransmitted":true,"doiAuthor":"Bratz K,G\u00f6lz G, Janczyk P, N\u00f6ckler K, Alter T","pdf":{"path":"http:\/\/data\/BMW_2015_0155.pdf","title":"BMW_2015_0155.pdf","description":"Analysis of in vitro and in vivo effects of probiotics against Campylobacter spp."},"authors":[{"firstName":"K","middleName":"","lastName":"Bratz"},{"firstName":"G","middleName":"","lastName":"G\u00f6lz"},{"firstName":"P","middleName":"","lastName":"Janczyk"},{"firstName":"K","middleName":"","lastName":"N\u00f6ckler"},{"firstName":"T","middleName":"","lastName":"Alter"}],"contentOptimised":"

Summary<\/strong><\/p>

Campylobacter (C.)<\/em> spp. are well recognised as the leading cause of bacterial foodborne diarrheal disease worldwide, with C. jejuni <\/em>and C. coli <\/em>as the most important species. C. col<\/em>i is highly abundant in pigs and pork meat has often been implicated as a source for human infection. Intestinal colonisation of C. coli<\/em> in pigs plays a role in carcass contamination during slaughter. Different pre-harvest intervention measures are proposed to reduce the C. coli<\/em> burden in the porcine intestine. Among others, the use of probiotics to prevent or reduce the colonisation of intestinal pathogens is discussed. One aim of this study was to screen a variety of probiotics to evaluate their inhibitory activity against Campylobacter <\/em>spp. in vitro. Therefore, cell-free culture supernatants of Lactobacillus<\/em> spp., Bifidobacterium<\/em> spp., Enterococcus (E.) faecium<\/em> NCIMB 10415, and Escherichia coli<\/em> Nissle 1917 were tested against C<\/em>. jejuni <\/em>and C. coli<\/em> by a well-diffusion agar assay. Seven out of eleven Lactobacillus <\/em>strains showed an inhibitory activity against at least one of the three tested Campylobacter <\/em>strains. This antagonistic activity against Campylobacter<\/em> spp. was caused by the production of organic acids that lowered the pH. Application with pH neutralised cell-free culture supernatants abolished this inhibitory effect. Other tested strains with probiotic properties showed no inhibitory activity against any Campylobacter <\/em>spp. strain. The strain E. faecium <\/em>NCIMB 10415 was chosen to test its inhibitory activity against C. coli <\/em>in vivo. Twenty weaned piglets were allocated into two groups, a probiotic group and a control group. The diet of the probiotic group was supplemented with E. faecium<\/em> NCIMB 10415 (109 cfu\/kg feed, Cylactin) since weaning, whereas the control group received no probiotic treatment. All piglets were naturally colonised with C. coli.<\/em> The excretion load of C. coli <\/em>was monitored for 28 days. The results indicate that dietary supplementation of E. faecium <\/em>NCIMB 10415 did not significantly affect C. coli<\/em> excretion levels in pigs. In this study, E. faecium<\/em> NCIMB 10415 showed no antagonistic activity against C. coli <\/em> in vitro and in vivo and had no impact on the growth performance of weaned piglets.<\/p>

Keywords:<\/strong><\/p>

Campylobacter<\/em> spp., C. coli<\/em>, probiotics, Enterococcus faecium<\/em>, pig<\/p>

Zusammenfassung<\/strong><\/p>

Campylobacter (C.) <\/em>spp. sind eine der h\u00e4ufigsten Ursachen f\u00fcr bakterielle lebensmittelassoziierte Infektionen weltweit. Hierbei stellen die Spezies C. jejuni<\/em> und C. coli<\/em> die wichtigsten Vertreter dar. C. coli <\/em>dominiert im Schwein, daher wird Schweinefleisch oft als Hauptquelle f\u00fcr C. coli-<\/em>Infektionen beim Menschen angesehen. Die Besiedlung des Darms mit C. coli <\/em>beim Schwein spielt bei der Kontamination des Schweinefleisches w\u00e4hrend der Schlachtung eine entscheidende Rolle. Der Einsatz verschiedener sogenannter Pre-harvest-Interventionsma\u00dfnahmen zur Reduktion der C. coli-<\/em>Belastung im Schwein wird diskutiert. Unter anderem wird die Anwendung probiotischer Bakterien zur Vermeidung oder Reduktion der Besiedlung intestinaler Pathogene im Schwein derzeit in Betracht gezogen. Ein Ziel dieser Studie war es, verschiedene Probiotika-St\u00e4mme auf ihre hemmende Wirkung gegen\u00fcber Campylobacter<\/em> spp. in vitro zu testen. Daf\u00fcr wurde die inhibierende Wirkung zellfreier Kultur-\u00dcberst\u00e4nde von Lactobacillus<\/em> spp. Bifidobacterium <\/em> spp., Enterococcus (E.) faecium <\/em>NCIMB 10415 sowie Escherichia coli<\/em> Nissle 1917 gegen\u00fcber C.<\/em> jejuni<\/em> und C. coli<\/em> mittels Agar-Diffusionstest \u00fcberpr\u00fcft. Bei sieben von elf Lactobacillus<\/em>-St\u00e4mmen konnte eine inhibierende Wirkung gegen mindestens einen der drei getesteten Campylobacter<\/em>-St\u00e4mme nachgewiesen werden. Dieser antagonistische Effekt kann auf die Produktion von organischen S\u00e4uren und dem damit verminderten pH-Wert zur\u00fcckgef\u00fchrt werden. Der Einsatz eines pH neutralisierten zellfreien Kultur-\u00dcberstandes hob diesen inhibierenden Effekt auf. Andere getestete St\u00e4mme mit probiotischen Eigenschaften zeigten hingegen keine inhibierende Wirkung gegen\u00fcber Campylobacter<\/em> spp. E. faecium<\/em> NCIMB 10415 wurde zur Testung seiner inhibierenden Wirkung gegen\u00fcber C. coli <\/em>f\u00fcr den Einsatz im Tierversuch ausgew\u00e4hlt. Daf\u00fcr wurden 20 abgesetzte Ferkel auf zwei Gruppen aufgeteilt, einer probiotischen Gruppe und einer Kontrollgruppe. Der probiotischen Gruppe wurde bereits w\u00e4hrend der Saugphase das Probiotikum E. faecium<\/em> NCIMB 10415 (109 cfu\/kg Futter, Cylactin) im Futter angeboten, wohingegen bei der Kontrollgruppe keine probiotische Zugabe erfolgte. Alle Ferkel waren auf nat\u00fcrliche Weise mit C. coli<\/em> besiedelt. Die Ausscheidung von C. coli<\/em> wurde f\u00fcr 28 Tage kontrolliert. Die Ergebnisse zeigen, dass die Verf\u00fctterung von E. faecium<\/em> NCIMB 10415 keinen signifikanten Einfluss auf die ausgeschiedene C. coli-<\/em>Zellzahl der Ferkel hat. In dieser Studie konnte keine antagonistische Wirkung von E. faecium<\/em> NCIMB 10415 gegen\u00fcber C. coli<\/em> in vitro und in vivo gezeigt werden. Die Wachstumsleistung entw\u00f6hnter Ferkel konnte durch das Probiotikum nicht gesteigert werden.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong><\/p>

Campylobacter <\/em>spp., Campylobacter coli<\/em>, Probiotikum, Enterococcus faecium<\/em>, Schwein<\/p>","primaryLanguage":"englisch","summary":"<\/p>

","keywords":["Campylobacter spp.","C. coli","probiotics","Enterococcus faecium","pig"],"zusammenfassung":"<\/p>

","schluesselwoerter":["Campylobacter spp.","Campylobacter coli","Probiotikum","Enterococcus faecium","Schwein"],"translatedTitle":"Analyse der In-vitro- und In-vivo-Effekte von Probiotika gegen Campylobacter spp.","abstractE":" Campylobacter (C.) spp. are well recognised as the leading cause of bacterial foodborne diarrheal disease worldwide, with C. jejuni and C. coli as the most important species. C. coli is highly abundant in pigs and pork meat has often been implicated as a source for human infection. Intestinal colonisation of C. coli in pigs plays a role in carcass contamination during slaughter. Different pre-harvest intervention measures are proposed to reduce the C. coli burden in the porcine intestine. Among others, the use of probiotics to prevent or reduce the colonisation of intestinal pathogens is discussed. One aim of this study was to screen a variety of probiotics to evaluate their inhibitory activity against Campylobacter spp. in vitro. Therefore, cell-free culture supernatants of Lactobacillus spp., Bifidobacterium spp., Enterococcus (E.) faecium NCIMB 10415, and Escherichia coli Nissle 1917 were tested against C. jejuni and C. coli by a well-diffusion agar assay. Seven out of eleven Lactobacillus strains showed an inhibitory activity against at least one of the three tested Campylobacter strains. This antagonistic activity against Campylobacter spp. was caused by the production of organic acids that lowered the pH. Application with pH neutralised cell-free culture supernatants abolished this inhibitory effect. Other tested strains with probiotic properties showed no inhibitory activity against any Campylobacter spp. strain. The strain E. faecium NCIMB 10415 was chosen to test its inhibitory activity against C. coli in vivo. Twenty weaned piglets were allocated into two groups, a probiotic group and a control group. The diet of the probiotic group was supplemented with E. faecium NCIMB 10415 (109 cfu\/kg feed, Cylactin) since weaning, whereas the control group received no probiotic treatment. All piglets were naturally colonised with C. coli. The excretion load of C. coli was monitored for 28 days. The results indicate that dietary supplementation of E. faecium NCIMB 10415 did not significantly affect C. coli excretion levels in pigs. In this study, E. faecium NCIMB 10415 showed no antagonistic activity against C. coli in vitro and in vivo and had no impact on the growth performance of weaned piglets.","date":{"year":2014,"date":"12\/2014","accepted":"2014-12-19"},"volume":"128","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"155-162","redirects":["analysis-of-in-vitro-and-in-vivo-effects-of-probiotics-against-campylobacter-spp\/150\/3216\/84333"],"tierartCategories":[],"artikelartCategories":["Open Access","Abostufe frei"]} CY - Hannover DA - 12/2014 DO - 10.2376/0005-9366-128-155 LA - English N2 - Campylobacter (C.) spp. are well recognised as the leading cause of bacterial foodborne diarrheal disease worldwide, with C. jejuni and C. coli as the most important species. C. coli is highly abundant in pigs and pork meat has often been implicated as a source for human infection. Intestinal colonisation of C. coli in pigs plays a role in carcass contamination during slaughter. Different pre-harvest intervention measures are proposed to reduce the C. coli burden in the porcine intestine. Among others, the use of probiotics to prevent or reduce the colonisation of intestinal pathogens is discussed. One aim of this study was to screen a variety of probiotics to evaluate their inhibitory activity against Campylobacter spp. in vitro. Therefore, cell-free culture supernatants of Lactobacillus spp., Bifidobacterium spp., Enterococcus (E.) faecium NCIMB 10415, and Escherichia coli Nissle 1917 were tested against C. jejuni and C. coli by a well-diffusion agar assay. Seven out of eleven Lactobacillus strains showed an inhibitory activity against at least one of the three tested Campylobacter strains. This antagonistic activity against Campylobacter spp. was caused by the production of organic acids that lowered the pH. Application with pH neutralised cell-free culture supernatants abolished this inhibitory effect. Other tested strains with probiotic properties showed no inhibitory activity against any Campylobacter spp. strain. The strain E. faecium NCIMB 10415 was chosen to test its inhibitory activity against C. coli in vivo. Twenty weaned piglets were allocated into two groups, a probiotic group and a control group. The diet of the probiotic group was supplemented with E. faecium NCIMB 10415 (109 cfu/kg feed, Cylactin) since weaning, whereas the control group received no probiotic treatment. All piglets were naturally colonised with C. coli. The excretion load of C. coli was monitored for 28 days. The results indicate that dietary supplementation of E. faecium NCIMB 10415 did not significantly affect C. coli excretion levels in pigs. In this study, E. faecium NCIMB 10415 showed no antagonistic activity against C. coli in vitro and in vivo and had no impact on the growth performance of weaned piglets. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2014 SP - 155 EP - 162 T1 - Analysis of in vitro and in vivo effects of probiotics against Campylobacter spp. T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Analysis of in vitro and in vivo effects of probiotics against Campylobacter spp. TT - Analyse der In-vitro- und In-vivo-Effekte von Probiotika gegen Campylobacter spp. VL - 128 SN - 0005-9366 ER -