TY - JOUR
KW - Campylobacter jejuni
KW - Autoinducer-2
KW - internalisation
AU - L Adler
AU - T Alter
AU - S Sharbati
AU - G Gölz
AB -  Bacteria recognise and constantly adjust to changing situations by sensing environmental  and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule,  found in many bacterial species and thus proposed to enable interspecies communication.  Two classes of AI-2 receptors have been identified so far. One class  of AI-2 receptors sense AI-2 by a two component signalling system. The other  class, the LsrB family, internalises and phosphorylates AI-2. Campylobacter (C.)  jejuni is known to produce AI-2, but no AI-2 receptor in C. jejuni has been found  yet. Therefore, all research on AI-2 dependent phenotypes has been conducted  with AI-2 synthase (luxS) mutants. This mutation also leads to a disruption of the  activated methyl cycle. Most studies lack sufficient complementation resulting  in not knowing whether phenotypes of luxS mutants depend on disrupted  metabolism or lack of AI-2. All this contributes to an intensive discussion about  the exact role of AI-2 in C. jejuni. Our previous study showed altering phenotypes  (growth and motility) of the C. jejuni NCTC 11168ΔluxS mutant in contrast to  wild type, which could be complemented with synthetic AI-2. To assess how AI-2  induces the altering phenotypes during complementation assays, we performed  an AI-2 uptake assay. Our data show, that AI-2 is not internalised by C. jejuni. These  data suggest, that yet unknown AI-2 receptors are present on the cell surface of  C. jejuni. Therefore further search of AI-2 receptors in C. jejuni should focus on two  component signalling systems and not on transporter systems.
BT - Berliner und Münchener Tierärztliche Wochenschrift
C1 - {"oldId":84328,"title":"The signalling molecule Autoinducer-2 is not internalised in Campylobacter jejuni","topline":"Open Access | CARO-Themenheft","teaserText":"Das Signalmolek\u00fcl Autoinducer-2 wird in Campylobacter jejuni nicht internalisiert","content":"<p> <span class=\"bold\">Summary<\/span><\/p><p> Bacteria recognise and constantly adjust to changing situations by sensing environmental  and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule,  found in many bacterial species and thus proposed to enable interspecies communication.  Two classes of AI-2 receptors have been identified so far. One class  of AI-2 receptors sense AI-2 by a two component signalling system. The other  class, the LsrB family, internalises and phosphorylates AI-2. <span class=\"italic\">Campylobacter (C.)  jejuni<\/span> is known to produce AI-2, but no AI-2 receptor in <span class=\"italic\">C. jejuni<\/span> has been found  yet. Therefore, all research on AI-2 dependent phenotypes has been conducted  with AI-2 synthase (<span class=\"italic\">luxS<\/span>) mutants. This mutation also leads to a disruption of the  activated methyl cycle. Most studies lack sufficient complementation resulting  in not knowing whether phenotypes of <span class=\"italic\">luxS<\/span> mutants depend on disrupted  metabolism or lack of AI-2. All this contributes to an intensive discussion about  the exact role of AI-2 in <span class=\"italic\">C. jejuni<\/span>. Our previous study showed altering phenotypes  (growth and motility) of the <span class=\"italic\">C. jejuni<\/span> NCTC 11168\u0394luxS mutant in contrast to  wild type, which could be complemented with synthetic AI-2. To assess how AI-2  induces the altering phenotypes during complementation assays, we performed  an AI-2 uptake assay. Our data show, that AI-2 is not internalised by <span class=\"italic\">C. jejuni.<\/span> These  data suggest, that yet unknown AI-2 receptors are present on the cell surface of  <span class=\"italic\">C. jejuni.<\/span> Therefore further search of AI-2 receptors in <span class=\"italic\">C. jejuni <\/span>should focus on two  component signalling systems and not on transporter systems.<\/p><p> <span class=\"bold\">Keywords<\/span><\/p><p><span class=\"italic\">Campylobacter jejuni<\/span>, Autoinducer-2, internalisation<\/p><p><span class=\"bold\">Zusammenfassung<\/span><\/p><p> Bakterien sind in der Lage, ver\u00e4ndernde Umweltbedingungen zu erkennen und  sich diesen anzupassen, indem sie \u00e4u\u00dfere Umwelteinfl\u00fcsse und selbst produzierte  Signale wahrnehmen. Autoinducer-2 (AI-2) ist ein Signalmolek\u00fcl, das von  vielen Bakterienspezies synthetisiert wird. Daher wird vermutet, dass AI-2 der  interspezies-spezifischen Kommunikation dient. Bisher sind zwei Klassen von AI-2  Rezeptoren eindeutig identifiziert. Eine Klasse von AI-2 Rezeptoren bindet AI-2  \u00fcber ein Zwei-Komponenten-Signalsystem. Die andere Klasse (LsrB) internalisiert  und phosphoryliert AI-2.<span class=\"italic\"> Campylobacter (C.) jejuni<\/span> synthetisiert AI-2, allerdings  konnte bislang noch kein Rezeptor in <span class=\"italic\">C. jejuni<\/span> identifiziert werden. Daher wurden  s\u00e4mtliche Studien von AI-2-abh\u00e4ngigen Ph\u00e4notypen mit AI-2-Synthase (<span class=\"italic\">luxS<\/span>)-Mutanten durchgef\u00fchrt. Allerdings spielt LuxS auch eine zentrale Rolle bei der  Regeneration von Homocystein im Methioninzyklus. In vielen Studien fehlt dabei  eine ausreichende Komplementation, um zu zeigen, ob auftretende Ph\u00e4notypen  ein Resultat der St\u00f6rung der metabolischen Funktion von LuxS sind oder sich  diese als Konsequenz der Unterbrechung der AI-2 vermittelten Zellkommunikation  zeigen. Eine fr\u00fchere Studie zeigte, dass unterschiedliche Ph\u00e4notypen  von <span class=\"italic\">C. jejuni <\/span>NCTC 11168\u0394<span class=\"italic\">luxS<\/span> Mutanten (Wachstum und Schw\u00e4rmverhalten)  im Vergleich zum Wildtyp auftreten. Diese Unterschiede konnten teilweise mit  synthetischem AI-2 komplementiert werden. Um zu untersuchen, wie AI-2 diese ver\u00e4nderten Ph\u00e4notypen w\u00e4hrend der Komplementation induziert, wurde hier  ein AI-2 uptake assay durchgef\u00fchrt. Dabei zeigte sich, dass AI-2 nicht aktiv in  <span class=\"italic\">C. jejuni <\/span>internalisiert wird. Unsere Daten lassen vermuten, dass noch unbekannte  AI-2 Rezeptoren in <span class=\"italic\">C. jejuni<\/span> vorhanden sind. Somit sollte sich die weitere Suche  nach AI-2 Rezeptoren in <span class=\"italic\">C. jejuni<\/span> auf Zwei-Komponenten-Systeme konzentrieren  und nicht auf Transportersysteme.<\/p><p> <span class=\"bold\">Schl\u00fcsselw\u00f6rter<\/span><\/p><p> <span class=\"italic\">Campylobacter jejuni<\/span>, Autoinducer 2, Internalisierung<\/p>","categories":["Open Access","Abostufe frei"],"fromDate":"Dec 19, 2014 8:29:35 AM","oldUrls":["http:\/\/vetline.de\/the-signalling-molecule-autoinducer-2-is-not-internalised-in-campylobacter-jejuni\/150\/3216\/84328"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr","doiDocumentUri":"http:\/\/cf01.vetline.schluetersche.de\/files\/smfiledata\/4\/3\/2\/7\/3\/0\/BMW_2015_Caro_14093_Adler_onl300.pdf","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 128, 111\u2013116","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-111","doiFirstPage":"111","doiLastPage":"116","doiTransmitted":true,"doiAuthor":"Adler L, Alter T, Sharbati S, G\u00f6lz G","pdf":{"path":"http:\/\/data\/BMW_2015_0111.pdf","title":"BMW_2015_0111.pdf","description":"The signalling molecule Autoinducer-2 is not internalised in Campylobacter jejuni"},"authors":[{"firstName":"L","middleName":"","lastName":"Adler"},{"firstName":"T","middleName":"","lastName":"Alter"},{"firstName":"S","middleName":"","lastName":"Sharbati"},{"firstName":"G","middleName":"","lastName":"G\u00f6lz"}],"contentOptimised":"<p> <strong>Summary<\/strong><\/p><p> Bacteria recognise and constantly adjust to changing situations by sensing environmental  and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule,  found in many bacterial species and thus proposed to enable interspecies communication.  Two classes of AI-2 receptors have been identified so far. One class  of AI-2 receptors sense AI-2 by a two component signalling system. The other  class, the LsrB family, internalises and phosphorylates AI-2. <em>Campylobacter (C.)  jejuni<\/em> is known to produce AI-2, but no AI-2 receptor in <em>C. jejuni<\/em> has been found  yet. Therefore, all research on AI-2 dependent phenotypes has been conducted  with AI-2 synthase (<em>luxS<\/em>) mutants. This mutation also leads to a disruption of the  activated methyl cycle. Most studies lack sufficient complementation resulting  in not knowing whether phenotypes of <em>luxS<\/em> mutants depend on disrupted  metabolism or lack of AI-2. All this contributes to an intensive discussion about  the exact role of AI-2 in <em>C. jejuni<\/em>. Our previous study showed altering phenotypes  (growth and motility) of the <em>C. jejuni<\/em> NCTC 11168\u0394luxS mutant in contrast to  wild type, which could be complemented with synthetic AI-2. To assess how AI-2  induces the altering phenotypes during complementation assays, we performed  an AI-2 uptake assay. Our data show, that AI-2 is not internalised by <em>C. jejuni.<\/em> These  data suggest, that yet unknown AI-2 receptors are present on the cell surface of  <em>C. jejuni.<\/em> Therefore further search of AI-2 receptors in <em>C. jejuni <\/em>should focus on two  component signalling systems and not on transporter systems.<\/p><p> <strong>Keywords:<\/strong><\/p><p><em>Campylobacter jejuni<\/em>, Autoinducer-2, internalisation<\/p><p><strong>Zusammenfassung<\/strong><\/p><p> Bakterien sind in der Lage, ver\u00e4ndernde Umweltbedingungen zu erkennen und  sich diesen anzupassen, indem sie \u00e4u\u00dfere Umwelteinfl\u00fcsse und selbst produzierte  Signale wahrnehmen. Autoinducer-2 (AI-2) ist ein Signalmolek\u00fcl, das von  vielen Bakterienspezies synthetisiert wird. Daher wird vermutet, dass AI-2 der  interspezies-spezifischen Kommunikation dient. Bisher sind zwei Klassen von AI-2  Rezeptoren eindeutig identifiziert. Eine Klasse von AI-2 Rezeptoren bindet AI-2  \u00fcber ein Zwei-Komponenten-Signalsystem. Die andere Klasse (LsrB) internalisiert  und phosphoryliert AI-2.<em> Campylobacter (C.) jejuni<\/em> synthetisiert AI-2, allerdings  konnte bislang noch kein Rezeptor in <em>C. jejuni<\/em> identifiziert werden. Daher wurden  s\u00e4mtliche Studien von AI-2-abh\u00e4ngigen Ph\u00e4notypen mit AI-2-Synthase (<em>luxS<\/em>)-Mutanten durchgef\u00fchrt. Allerdings spielt LuxS auch eine zentrale Rolle bei der  Regeneration von Homocystein im Methioninzyklus. In vielen Studien fehlt dabei  eine ausreichende Komplementation, um zu zeigen, ob auftretende Ph\u00e4notypen  ein Resultat der St\u00f6rung der metabolischen Funktion von LuxS sind oder sich  diese als Konsequenz der Unterbrechung der AI-2 vermittelten Zellkommunikation  zeigen. Eine fr\u00fchere Studie zeigte, dass unterschiedliche Ph\u00e4notypen  von <em>C. jejuni <\/em>NCTC 11168\u0394<em>luxS<\/em> Mutanten (Wachstum und Schw\u00e4rmverhalten)  im Vergleich zum Wildtyp auftreten. Diese Unterschiede konnten teilweise mit  synthetischem AI-2 komplementiert werden. Um zu untersuchen, wie AI-2 diese ver\u00e4nderten Ph\u00e4notypen w\u00e4hrend der Komplementation induziert, wurde hier  ein AI-2 uptake assay durchgef\u00fchrt. Dabei zeigte sich, dass AI-2 nicht aktiv in  <em>C. jejuni <\/em>internalisiert wird. Unsere Daten lassen vermuten, dass noch unbekannte  AI-2 Rezeptoren in <em>C. jejuni<\/em> vorhanden sind. Somit sollte sich die weitere Suche  nach AI-2 Rezeptoren in <em>C. jejuni<\/em> auf Zwei-Komponenten-Systeme konzentrieren  und nicht auf Transportersysteme.<\/p><p> <strong>Schl\u00fcsselw\u00f6rter:<\/strong><\/p><p> <em>Campylobacter jejuni<\/em>, Autoinducer 2, Internalisierung<\/p>","primaryLanguage":"englisch","summary":"<\/p><p> Bacteria recognise and constantly adjust to changing situations by sensing environmental  and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule,  found in many bacterial species and thus proposed to enable interspecies communication.  Two classes of AI-2 receptors have been identified so far. One class  of AI-2 receptors sense AI-2 by a two component signalling system. The other  class, the LsrB family, internalises and phosphorylates AI-2. <em>Campylobacter (C.)  jejuni<\/em> is known to produce AI-2, but no AI-2 receptor in <em>C. jejuni<\/em> has been found  yet. Therefore, all research on AI-2 dependent phenotypes has been conducted  with AI-2 synthase (<em>luxS<\/em>) mutants. This mutation also leads to a disruption of the  activated methyl cycle. Most studies lack sufficient complementation resulting  in not knowing whether phenotypes of <em>luxS<\/em> mutants depend on disrupted  metabolism or lack of AI-2. All this contributes to an intensive discussion about  the exact role of AI-2 in <em>C. jejuni<\/em>. Our previous study showed altering phenotypes  (growth and motility) of the <em>C. jejuni<\/em> NCTC 11168\u0394luxS mutant in contrast to  wild type, which could be complemented with synthetic AI-2. To assess how AI-2  induces the altering phenotypes during complementation assays, we performed  an AI-2 uptake assay. Our data show, that AI-2 is not internalised by <em>C. jejuni.<\/em> These  data suggest, that yet unknown AI-2 receptors are present on the cell surface of  <em>C. jejuni.<\/em> Therefore further search of AI-2 receptors in <em>C. jejuni <\/em>should focus on two  component signalling systems and not on transporter systems.<\/p><p>","keywords":["Campylobacter jejuni","Autoinducer-2","internalisation"],"zusammenfassung":"<\/p><p> Bakterien sind in der Lage, ver\u00e4ndernde Umweltbedingungen zu erkennen und  sich diesen anzupassen, indem sie \u00e4u\u00dfere Umwelteinfl\u00fcsse und selbst produzierte  Signale wahrnehmen. Autoinducer-2 (AI-2) ist ein Signalmolek\u00fcl, das von  vielen Bakterienspezies synthetisiert wird. Daher wird vermutet, dass AI-2 der  interspezies-spezifischen Kommunikation dient. Bisher sind zwei Klassen von AI-2  Rezeptoren eindeutig identifiziert. Eine Klasse von AI-2 Rezeptoren bindet AI-2  \u00fcber ein Zwei-Komponenten-Signalsystem. Die andere Klasse (LsrB) internalisiert  und phosphoryliert AI-2.<em> Campylobacter (C.) jejuni<\/em> synthetisiert AI-2, allerdings  konnte bislang noch kein Rezeptor in <em>C. jejuni<\/em> identifiziert werden. Daher wurden  s\u00e4mtliche Studien von AI-2-abh\u00e4ngigen Ph\u00e4notypen mit AI-2-Synthase (<em>luxS<\/em>)-Mutanten durchgef\u00fchrt. Allerdings spielt LuxS auch eine zentrale Rolle bei der  Regeneration von Homocystein im Methioninzyklus. In vielen Studien fehlt dabei  eine ausreichende Komplementation, um zu zeigen, ob auftretende Ph\u00e4notypen  ein Resultat der St\u00f6rung der metabolischen Funktion von LuxS sind oder sich  diese als Konsequenz der Unterbrechung der AI-2 vermittelten Zellkommunikation  zeigen. Eine fr\u00fchere Studie zeigte, dass unterschiedliche Ph\u00e4notypen  von <em>C. jejuni <\/em>NCTC 11168\u0394<em>luxS<\/em> Mutanten (Wachstum und Schw\u00e4rmverhalten)  im Vergleich zum Wildtyp auftreten. Diese Unterschiede konnten teilweise mit  synthetischem AI-2 komplementiert werden. Um zu untersuchen, wie AI-2 diese ver\u00e4nderten Ph\u00e4notypen w\u00e4hrend der Komplementation induziert, wurde hier  ein AI-2 uptake assay durchgef\u00fchrt. Dabei zeigte sich, dass AI-2 nicht aktiv in  <em>C. jejuni <\/em>internalisiert wird. Unsere Daten lassen vermuten, dass noch unbekannte  AI-2 Rezeptoren in <em>C. jejuni<\/em> vorhanden sind. Somit sollte sich die weitere Suche  nach AI-2 Rezeptoren in <em>C. jejuni<\/em> auf Zwei-Komponenten-Systeme konzentrieren  und nicht auf Transportersysteme.<\/p><p>","schluesselwoerter":["Campylobacter jejuni","Autoinducer 2","Internalisierung"],"translatedTitle":"Das Signalmolek\u00fcl Autoinducer-2 wird in Campylobacter jejuni nicht internalisiert","abstractE":" Bacteria recognise and constantly adjust to changing situations by sensing environmental  and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule,  found in many bacterial species and thus proposed to enable interspecies communication.  Two classes of AI-2 receptors have been identified so far. One class  of AI-2 receptors sense AI-2 by a two component signalling system. The other  class, the LsrB family, internalises and phosphorylates AI-2. Campylobacter (C.)  jejuni is known to produce AI-2, but no AI-2 receptor in C. jejuni has been found  yet. Therefore, all research on AI-2 dependent phenotypes has been conducted  with AI-2 synthase (luxS) mutants. This mutation also leads to a disruption of the  activated methyl cycle. Most studies lack sufficient complementation resulting  in not knowing whether phenotypes of luxS mutants depend on disrupted  metabolism or lack of AI-2. All this contributes to an intensive discussion about  the exact role of AI-2 in C. jejuni. Our previous study showed altering phenotypes  (growth and motility) of the C. jejuni NCTC 11168\u0394luxS mutant in contrast to  wild type, which could be complemented with synthetic AI-2. To assess how AI-2  induces the altering phenotypes during complementation assays, we performed  an AI-2 uptake assay. Our data show, that AI-2 is not internalised by C. jejuni. These  data suggest, that yet unknown AI-2 receptors are present on the cell surface of  C. jejuni. Therefore further search of AI-2 receptors in C. jejuni should focus on two  component signalling systems and not on transporter systems.","date":{"year":2014,"date":"12\/2014","accepted":"2014-12-19"},"volume":"128","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"111-116","redirects":["the-signalling-molecule-autoinducer-2-is-not-internalised-in-campylobacter-jejuni\/150\/3216\/84328"],"tierartCategories":[],"artikelartCategories":["Open Access","Abostufe frei"]}
CY - Hannover
DA - 12/2014
DO - 10.2376/0005-9366-128-111
LA - English
N2 -  Bacteria recognise and constantly adjust to changing situations by sensing environmental  and self-produced signals. Autoinducer-2 (AI-2) is a signal molecule,  found in many bacterial species and thus proposed to enable interspecies communication.  Two classes of AI-2 receptors have been identified so far. One class  of AI-2 receptors sense AI-2 by a two component signalling system. The other  class, the LsrB family, internalises and phosphorylates AI-2. Campylobacter (C.)  jejuni is known to produce AI-2, but no AI-2 receptor in C. jejuni has been found  yet. Therefore, all research on AI-2 dependent phenotypes has been conducted  with AI-2 synthase (luxS) mutants. This mutation also leads to a disruption of the  activated methyl cycle. Most studies lack sufficient complementation resulting  in not knowing whether phenotypes of luxS mutants depend on disrupted  metabolism or lack of AI-2. All this contributes to an intensive discussion about  the exact role of AI-2 in C. jejuni. Our previous study showed altering phenotypes  (growth and motility) of the C. jejuni NCTC 11168ΔluxS mutant in contrast to  wild type, which could be complemented with synthetic AI-2. To assess how AI-2  induces the altering phenotypes during complementation assays, we performed  an AI-2 uptake assay. Our data show, that AI-2 is not internalised by C. jejuni. These  data suggest, that yet unknown AI-2 receptors are present on the cell surface of  C. jejuni. Therefore further search of AI-2 receptors in C. jejuni should focus on two  component signalling systems and not on transporter systems.
PB - Schlütersche Verlagsgesellschaft mbH & Co. KG
PP - Hannover
PY - 2014
SP - 111
EP - 116
T1 - The signalling molecule Autoinducer-2 is not internalised in Campylobacter jejuni
T2 - Berliner und Münchener Tierärztliche Wochenschrift
TI - The signalling molecule Autoinducer-2 is not internalised in Campylobacter jejuni
TT - Das Signalmolekül Autoinducer-2 wird in Campylobacter jejuni nicht internalisiert
VL - 128
SN - 0005-9366
ER -