TY - JOUR KW - Arcanobacterium haemolyticum KW - donkey KW - MALDI-TOF MS KW - 16S rDNA KW - gap KW - virulence genes AU - O Sammra AU - A Balbutskaya AU - S Nagib AU - J Alber AU - C Lämmler AU - A Abdulmawjood AU - M Timke AU - M Kostrzewa AU - E Prenger-Berninghoff AB - The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus β-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":76066,"title":"Properties of an Arcanobacterium haemolyticum strain isolated from a donkey","topline":"","teaserText":"Eigenschaften eines von einem Esel isolierten Arcanobacterium haemolyticum-Stammes","content":"

Summary <\/span>
The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus \u03b2-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey.<\/p>

Keywords<\/span>
Arcanobacterium haemolyticum, donkey, MALDI-TOF MS, 16S rDNA, gap, virulence genes<\/p>

Zusammenfassung <\/span>
In der vorliegenden Arbeit konnte der Arcanobacterium haemolyticum-Stamm A. haemolyticum P646, isoliert aus dem eitrigen Nasenausfluss eines Esels, ph\u00e4notypisch und genotypisch charakterisiert werden. A. haemolyticum P646 zeigte auf Kaninchenblutagarplatten, im Vergleich zu Schafblutagarplatten, eine verst\u00e4rkte H\u00e4molyse, eine synergistische, CAMP-\u00e4hnliche Reaktion mit Streptococcus agalactiae und Rhodococcus equi als Indikatorst\u00e4mmen, eine umgekehrte CAMP-Reaktion in der Zone des Staphylococcus aureus-\u03b2-H\u00e4molysins und die typischen biochemischen Eigenschaften dieser Spezies. Die Spezieszuordnung erfolgte im Weiteren durch MALDI-TOF-Massenspektrometrie, durch Sequenzierung der 16S rDNA und des Glyceraldehyd-3-Phosphat-Dehydrogenase kodierenden Gens gap und durch Amplifizierung eines A. haemolyticum-spezifischen Teils der 16S-23S-rDNA-Intergenic-Spacer-Region und der 23S rDNA. A. haemolyticum P646 und der Referenzstamm A. haemolyticum DSM 20595 wurden im Weiteren charakterisiert durch Amplifizierung mutma\u00dflicher Virulenzgene. Die Virulenzgene kodieren Arcanolysin, Phospholipase D, H\u00e4molysin A, ein CAMP-Factor-Family-Protein, ein Kollagenbindendes Protein und zwei Neuraminidasen, die f\u00fcr A. haemolyticum DSM 20595 nachgewiesen wurden. A. haemolyticum P646 zeigte ein vergleichbares Genspektrum, war aber negativ f\u00fcr die Gene, die das Kollagen-bindende Protein und die Neuraminidase H kodieren. Nach vorliegendem Wissen ist dies die erste ph\u00e4notypische und genotypische Charakterisierung eines von einem Esel isolierten A. haemolyticum-Stammes.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Arcanobacterium haemolyticum, Esel, MALDI-TOF-MS, 16S rDNA, gap, Virulenzgene<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Feb 28, 2014 11:00:00 PM","oldUrls":["http:\/\/vetline.de\/properties-of-an-arcanobacterium-haemolyticum-strain-isolated-from-a-donkey\/150\/3130\/76066"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/vetline.de\/properties-of-an-arcanobacterium-haemolyticum-strain-isolated-from-a-donkey\/150\/3130\/76066\/","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr 127: 56-60","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-127-56","doiFirstPage":"56","doiLastPage":"60","doiTransmitted":true,"doiAuthor":"Sammra O, Balbutskaya A, Nagib S, Alber J, L\u00e4mmler C, Abdulmawjood A, Timke M, Kostrzewa M, Prenger-Berninghoff E","pdf":{"path":"http:\/\/data\/BMTW_2014_01_02_0056_ges72.pdf","title":"BMTW_2014_01_0056.pdf","description":"Properties of an Arcanobacterium haemolyticum strain isolated from a donkey"},"authors":[{"firstName":"O","middleName":"","lastName":"Sammra"},{"firstName":"A","middleName":"","lastName":"Balbutskaya"},{"firstName":"S","middleName":"","lastName":"Nagib"},{"firstName":"J","middleName":"","lastName":"Alber"},{"firstName":"C","middleName":"","lastName":"L\u00e4mmler"},{"firstName":"A","middleName":"","lastName":"Abdulmawjood"},{"firstName":"M","middleName":"","lastName":"Timke"},{"firstName":"M","middleName":"","lastName":"Kostrzewa"},{"firstName":"E","middleName":"","lastName":"Prenger-Berninghoff"}],"contentOptimised":"

Summary<\/strong>
The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus \u03b2-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey.<\/p>

Keywords:<\/strong>
Arcanobacterium haemolyticum, donkey, MALDI-TOF MS, 16S rDNA, gap, virulence genes<\/p>

Zusammenfassung<\/strong>
In der vorliegenden Arbeit konnte der Arcanobacterium haemolyticum-Stamm A. haemolyticum P646, isoliert aus dem eitrigen Nasenausfluss eines Esels, ph\u00e4notypisch und genotypisch charakterisiert werden. A. haemolyticum P646 zeigte auf Kaninchenblutagarplatten, im Vergleich zu Schafblutagarplatten, eine verst\u00e4rkte H\u00e4molyse, eine synergistische, CAMP-\u00e4hnliche Reaktion mit Streptococcus agalactiae und Rhodococcus equi als Indikatorst\u00e4mmen, eine umgekehrte CAMP-Reaktion in der Zone des Staphylococcus aureus-\u03b2-H\u00e4molysins und die typischen biochemischen Eigenschaften dieser Spezies. Die Spezieszuordnung erfolgte im Weiteren durch MALDI-TOF-Massenspektrometrie, durch Sequenzierung der 16S rDNA und des Glyceraldehyd-3-Phosphat-Dehydrogenase kodierenden Gens gap und durch Amplifizierung eines A. haemolyticum-spezifischen Teils der 16S-23S-rDNA-Intergenic-Spacer-Region und der 23S rDNA. A. haemolyticum P646 und der Referenzstamm A. haemolyticum DSM 20595 wurden im Weiteren charakterisiert durch Amplifizierung mutma\u00dflicher Virulenzgene. Die Virulenzgene kodieren Arcanolysin, Phospholipase D, H\u00e4molysin A, ein CAMP-Factor-Family-Protein, ein Kollagenbindendes Protein und zwei Neuraminidasen, die f\u00fcr A. haemolyticum DSM 20595 nachgewiesen wurden. A. haemolyticum P646 zeigte ein vergleichbares Genspektrum, war aber negativ f\u00fcr die Gene, die das Kollagen-bindende Protein und die Neuraminidase H kodieren. Nach vorliegendem Wissen ist dies die erste ph\u00e4notypische und genotypische Charakterisierung eines von einem Esel isolierten A. haemolyticum-Stammes.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Arcanobacterium haemolyticum, Esel, MALDI-TOF-MS, 16S rDNA, gap, Virulenzgene<\/p>","primaryLanguage":"englisch","summary":"The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus \u03b2-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey.<\/p>

","keywords":["Arcanobacterium haemolyticum","donkey","MALDI-TOF MS","16S rDNA","gap","virulence genes"],"zusammenfassung":"In der vorliegenden Arbeit konnte der Arcanobacterium haemolyticum-Stamm A. haemolyticum P646, isoliert aus dem eitrigen Nasenausfluss eines Esels, ph\u00e4notypisch und genotypisch charakterisiert werden. A. haemolyticum P646 zeigte auf Kaninchenblutagarplatten, im Vergleich zu Schafblutagarplatten, eine verst\u00e4rkte H\u00e4molyse, eine synergistische, CAMP-\u00e4hnliche Reaktion mit Streptococcus agalactiae und Rhodococcus equi als Indikatorst\u00e4mmen, eine umgekehrte CAMP-Reaktion in der Zone des Staphylococcus aureus-\u03b2-H\u00e4molysins und die typischen biochemischen Eigenschaften dieser Spezies. Die Spezieszuordnung erfolgte im Weiteren durch MALDI-TOF-Massenspektrometrie, durch Sequenzierung der 16S rDNA und des Glyceraldehyd-3-Phosphat-Dehydrogenase kodierenden Gens gap und durch Amplifizierung eines A. haemolyticum-spezifischen Teils der 16S-23S-rDNA-Intergenic-Spacer-Region und der 23S rDNA. A. haemolyticum P646 und der Referenzstamm A. haemolyticum DSM 20595 wurden im Weiteren charakterisiert durch Amplifizierung mutma\u00dflicher Virulenzgene. Die Virulenzgene kodieren Arcanolysin, Phospholipase D, H\u00e4molysin A, ein CAMP-Factor-Family-Protein, ein Kollagenbindendes Protein und zwei Neuraminidasen, die f\u00fcr A. haemolyticum DSM 20595 nachgewiesen wurden. A. haemolyticum P646 zeigte ein vergleichbares Genspektrum, war aber negativ f\u00fcr die Gene, die das Kollagen-bindende Protein und die Neuraminidase H kodieren. Nach vorliegendem Wissen ist dies die erste ph\u00e4notypische und genotypische Charakterisierung eines von einem Esel isolierten A. haemolyticum-Stammes.<\/p>

","schluesselwoerter":["Arcanobacterium haemolyticum","Esel","MALDI-TOF-MS","16S rDNA","gap","Virulenzgene"],"translatedTitle":"Eigenschaften eines von einem Esel isolierten Arcanobacterium haemolyticum-Stammes","abstractE":"The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus \u03b2-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey.","date":{"year":2014,"date":"02\/2014","accepted":"2014-02-28"},"volume":"127","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"56-60","redirects":["properties-of-an-arcanobacterium-haemolyticum-strain-isolated-from-a-donkey\/150\/3130\/76066"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 02/2014 DO - 10.2376/0005-9366-127-56 LA - English N2 - The present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus β-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2014 SP - 56 EP - 60 T1 - Properties of an Arcanobacterium haemolyticum strain isolated from a donkey T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Properties of an Arcanobacterium haemolyticum strain isolated from a donkey TT - Eigenschaften eines von einem Esel isolierten Arcanobacterium haemolyticum-Stammes VL - 127 SN - 0005-9366 ER -