TY - JOUR KW - Johne’s disease KW - Mycobacterium avium subsp. paratuberculosis KW - bacteriological culture KW - PCR KW - environmental sampling AU - J Köchler AU - S Gschaider AU - J Spergser AU - A Tichy AU - C Mader AU - M Vill AU - P Ortner AU - J Kössler AU - J Khol AB - Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis or Johne’s disease (JD) in cattle, leading to significant economic losses in the effected herds. Recently, boot swab samples have been used in cattle farms in order to establish the MAP-herd status. The aim of the present study was to evaluate the reproducibility of MAP-negative boot swab samples on cattle premises. Therefore, boot swab samples were collected from 158 farms with an average size of 16 cows, previously tested MAP-negative in boot swabs by the local veterinarians. Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). Boot swabs were tested for MAP by solid culture and PCR, leading to positive results in eight herds (5.1%). Culture and PCR showed a high correlation, as seven of the herds were positive both in culture and PCR, and one herd was positive in PCR only. Based on these results the reproducibility of a negative boot swab result was estimated as 24.2% (25% CI: 20%, 28%). No significant differences in the results between veterinarians with and without positive samples in the first survey were found, indicating no or little influence of the person collecting the boot swab samples. From the results of the study it is concluded that negative boot swab sample results have a high reproducibility for the detection of MAP in cattle herds in small structured agricultural systems. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":100034,"title":"Reproducibility of negative boot swab samples for paratuberculosis in cattle herds in Tyrol (Austria)","topline":"","teaserText":"Reproduzierbarkeit negativer Sockentupferergebnisse zur Bestimmung des Paratuberkulosestatus in Rinderbetrieben in Tirol (\u00d6sterreich)","content":"

Summary<\/span>
Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis or Johne\u2019s disease (JD) in cattle, leading to significant economic losses in the effected herds. Recently, boot swab samples have been used in cattle farms in order to establish the MAP-herd status. The aim of the present study was to evaluate the reproducibility of MAP-negative boot swab samples on cattle premises. Therefore, boot swab samples were collected from 158 farms with an average size of 16 cows, previously tested MAP-negative in boot swabs by the local veterinarians. Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). Boot swabs were tested for MAP by solid culture and PCR, leading to positive results in eight herds (5.1%). Culture and PCR showed a high correlation, as seven of the herds were positive both in culture and PCR, and one herd was positive in PCR only. Based on these results the reproducibility of a negative boot swab result was estimated as 24.2% (25% CI: 20%, 28%). No significant differences in the results between veterinarians with and without positive samples in the first survey were found, indicating no or little influence of the person collecting the boot swab samples. From the results of the study it is concluded that negative boot swab sample results have a high reproducibility for the detection of MAP in cattle herds in small structured agricultural systems.<\/p>

Keywords<\/span>
Johne\u2019s disease, Mycobacterium avium subsp. paratuberculosis, bacteriological culture, PCR, environmental sampling<\/p>

Zusammenfassung<\/span>
Die Paratuberkulose oder Johne\u00b4sche Krankheit wird durch Mycobacterium avium subsp. paratuberculosis (MAP) hervorgerufen und kann in betroffenen Rinderherden zu erheblichen wirtschaftlichen Verlusten f\u00fchren. Zur Erhebung des MAPHerdenstatus im Zuge von Kontroll- und \u00dcberwachungsprogrammen werden in Rinderbetrieben seit einiger Zeit Sockentupfer eingesetzt. Das Ziel dieser Untersuchung war es, die Reproduzierbarkeit negativer MAP-Sockentupferergebnisse in Rinderbetrieben zu evaluieren. Dazu wurden 158 Rinderbetriebe mit einer durchschnittlichen Herdengr\u00f6\u00dfe von 16 K\u00fchen, die bereits zuvor von den Betreuungstier\u00e4rzten mittels Sockentupfern negativ auf MAP getestet wurden, nachuntersucht. Die Herden wurden anhand von Risikofaktoren f\u00fcr das Vorliegen einer MAP-Infektion (74 Herden), den Ergebnissen der vorangegangenen Untersuchung von Tier\u00e4rzten, die ausschlie\u00dflich negative Ergebnisse aufwiesen (75 Herden), und nachgewiesenen klinischen Paratuberkulosef\u00e4llen (neun Herden) ausgew\u00e4hlt. Die Stiefeltupfer wurden mittels bakteriologischer Kultur und PCR untersucht, wobei acht Betriebe (5,1 %) ein positives Ergebnis aufwiesen. Die bakteriologische Kultur und die PCR zeigten eine hohe \u00dcbereinstimmung, sieben der Betriebe waren in beiden Tests positiv und lediglich in einem Betrieb lag nur in der PCR ein positives Ergebnis vor. Basierend auf diesen Ergebnissen konnte f\u00fcr die Stiefeltupfer ein negativer pr\u00e4diktiver Wert von 24,2 % (25%-CI: 20 %, 28 %) errechnet werden. Zwischen den Ergebnissen von Tier\u00e4rzten mit und ohne positiven Befund in der ersten Untersuchung lagen keine signifikanten Unterschiede vor, was auf einen zu vernachl\u00e4ssigenden Einfluss der durchf\u00fchrenden Person auf das Ergebnis der Sockentupfer hinweist. Die Ergebnisse der vorliegenden Untersuchung zeigen, dass negative Sockentupfer auch in kleinb\u00e4uerlichen Strukturen eine hohe Reproduzierbarkeit aufweisen. <\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Johne\u2018sche Krankheit, Mycobacterium avium subsp. paratuberculosis, bakteriologische Kultur, PCR, Umweltkotprobe<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jan 14, 2017 11:00:00 PM","oldUrls":["http:\/\/vetline.de\/reproducibility-of-negative-boot-swab-samples-for-paratuberculosis-in-cattle-herds-in-tyrol-austria\/150\/3130\/100034"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/reproducibility-of-negative-boot-swab-samples-for-paratuberculosis-in-cattle-herds-in-tyrol-austria\/150\/3130\/100034\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 130, Heft 1\/2 (2017), Seiten 29\u201333","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16027","doiFirstPage":"29","doiLastPage":"33","doiTransmitted":true,"doiAuthor":"K\u00f6chler J, Gschaider S, Spergser J, Tichy A, Mader C, Vill M, Ortner P, K\u00f6ssler J, Lorenz Khol J","pdf":{"path":"http:\/\/data\/BMW_2017_01_02_0029.pdf","title":"BMW_2017_01_02_0029.pdf","description":"Reproducibility of negative boot swab samples for paratuberculosis in cattle herds in Tyrol (Austria) "},"authors":[{"firstName":"J","middleName":"","lastName":"K\u00f6chler"},{"firstName":"S","middleName":"","lastName":"Gschaider"},{"firstName":"J","middleName":"","lastName":"Spergser"},{"firstName":"A","middleName":"","lastName":"Tichy"},{"firstName":"C","middleName":"","lastName":"Mader"},{"firstName":"M","middleName":"","lastName":"Vill"},{"firstName":"P","middleName":"","lastName":"Ortner"},{"firstName":"J","middleName":"","lastName":"K\u00f6ssler"},{"firstName":"J","middleName":"","lastName":"Lorenz Khol"}],"contentOptimised":"

Summary<\/strong>
Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis or Johne\u2019s disease (JD) in cattle, leading to significant economic losses in the effected herds. Recently, boot swab samples have been used in cattle farms in order to establish the MAP-herd status. The aim of the present study was to evaluate the reproducibility of MAP-negative boot swab samples on cattle premises. Therefore, boot swab samples were collected from 158 farms with an average size of 16 cows, previously tested MAP-negative in boot swabs by the local veterinarians. Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). Boot swabs were tested for MAP by solid culture and PCR, leading to positive results in eight herds (5.1%). Culture and PCR showed a high correlation, as seven of the herds were positive both in culture and PCR, and one herd was positive in PCR only. Based on these results the reproducibility of a negative boot swab result was estimated as 24.2% (25% CI: 20%, 28%). No significant differences in the results between veterinarians with and without positive samples in the first survey were found, indicating no or little influence of the person collecting the boot swab samples. From the results of the study it is concluded that negative boot swab sample results have a high reproducibility for the detection of MAP in cattle herds in small structured agricultural systems.<\/p>

Keywords:<\/strong>
Johne\u2019s disease, Mycobacterium avium subsp. paratuberculosis, bacteriological culture, PCR, environmental sampling<\/p>

Zusammenfassung<\/strong>
Die Paratuberkulose oder Johne\u00b4sche Krankheit wird durch Mycobacterium avium subsp. paratuberculosis (MAP) hervorgerufen und kann in betroffenen Rinderherden zu erheblichen wirtschaftlichen Verlusten f\u00fchren. Zur Erhebung des MAPHerdenstatus im Zuge von Kontroll- und \u00dcberwachungsprogrammen werden in Rinderbetrieben seit einiger Zeit Sockentupfer eingesetzt. Das Ziel dieser Untersuchung war es, die Reproduzierbarkeit negativer MAP-Sockentupferergebnisse in Rinderbetrieben zu evaluieren. Dazu wurden 158 Rinderbetriebe mit einer durchschnittlichen Herdengr\u00f6\u00dfe von 16 K\u00fchen, die bereits zuvor von den Betreuungstier\u00e4rzten mittels Sockentupfern negativ auf MAP getestet wurden, nachuntersucht. Die Herden wurden anhand von Risikofaktoren f\u00fcr das Vorliegen einer MAP-Infektion (74 Herden), den Ergebnissen der vorangegangenen Untersuchung von Tier\u00e4rzten, die ausschlie\u00dflich negative Ergebnisse aufwiesen (75 Herden), und nachgewiesenen klinischen Paratuberkulosef\u00e4llen (neun Herden) ausgew\u00e4hlt. Die Stiefeltupfer wurden mittels bakteriologischer Kultur und PCR untersucht, wobei acht Betriebe (5,1 %) ein positives Ergebnis aufwiesen. Die bakteriologische Kultur und die PCR zeigten eine hohe \u00dcbereinstimmung, sieben der Betriebe waren in beiden Tests positiv und lediglich in einem Betrieb lag nur in der PCR ein positives Ergebnis vor. Basierend auf diesen Ergebnissen konnte f\u00fcr die Stiefeltupfer ein negativer pr\u00e4diktiver Wert von 24,2 % (25%-CI: 20 %, 28 %) errechnet werden. Zwischen den Ergebnissen von Tier\u00e4rzten mit und ohne positiven Befund in der ersten Untersuchung lagen keine signifikanten Unterschiede vor, was auf einen zu vernachl\u00e4ssigenden Einfluss der durchf\u00fchrenden Person auf das Ergebnis der Sockentupfer hinweist. Die Ergebnisse der vorliegenden Untersuchung zeigen, dass negative Sockentupfer auch in kleinb\u00e4uerlichen Strukturen eine hohe Reproduzierbarkeit aufweisen. <\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Johne\u2018sche Krankheit, Mycobacterium avium subsp. paratuberculosis, bakteriologische Kultur, PCR, Umweltkotprobe<\/p>","primaryLanguage":"englisch","summary":"Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis or Johne\u2019s disease (JD) in cattle, leading to significant economic losses in the effected herds. Recently, boot swab samples have been used in cattle farms in order to establish the MAP-herd status. The aim of the present study was to evaluate the reproducibility of MAP-negative boot swab samples on cattle premises. Therefore, boot swab samples were collected from 158 farms with an average size of 16 cows, previously tested MAP-negative in boot swabs by the local veterinarians. Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). Boot swabs were tested for MAP by solid culture and PCR, leading to positive results in eight herds (5.1%). Culture and PCR showed a high correlation, as seven of the herds were positive both in culture and PCR, and one herd was positive in PCR only. Based on these results the reproducibility of a negative boot swab result was estimated as 24.2% (25% CI: 20%, 28%). No significant differences in the results between veterinarians with and without positive samples in the first survey were found, indicating no or little influence of the person collecting the boot swab samples. From the results of the study it is concluded that negative boot swab sample results have a high reproducibility for the detection of MAP in cattle herds in small structured agricultural systems.<\/p>

","keywords":["Johne\u2019s disease","Mycobacterium avium subsp. paratuberculosis","bacteriological culture","PCR","environmental sampling"],"zusammenfassung":"Die Paratuberkulose oder Johne\u00b4sche Krankheit wird durch Mycobacterium avium subsp. paratuberculosis (MAP) hervorgerufen und kann in betroffenen Rinderherden zu erheblichen wirtschaftlichen Verlusten f\u00fchren. Zur Erhebung des MAPHerdenstatus im Zuge von Kontroll- und \u00dcberwachungsprogrammen werden in Rinderbetrieben seit einiger Zeit Sockentupfer eingesetzt. Das Ziel dieser Untersuchung war es, die Reproduzierbarkeit negativer MAP-Sockentupferergebnisse in Rinderbetrieben zu evaluieren. Dazu wurden 158 Rinderbetriebe mit einer durchschnittlichen Herdengr\u00f6\u00dfe von 16 K\u00fchen, die bereits zuvor von den Betreuungstier\u00e4rzten mittels Sockentupfern negativ auf MAP getestet wurden, nachuntersucht. Die Herden wurden anhand von Risikofaktoren f\u00fcr das Vorliegen einer MAP-Infektion (74 Herden), den Ergebnissen der vorangegangenen Untersuchung von Tier\u00e4rzten, die ausschlie\u00dflich negative Ergebnisse aufwiesen (75 Herden), und nachgewiesenen klinischen Paratuberkulosef\u00e4llen (neun Herden) ausgew\u00e4hlt. Die Stiefeltupfer wurden mittels bakteriologischer Kultur und PCR untersucht, wobei acht Betriebe (5,1 %) ein positives Ergebnis aufwiesen. Die bakteriologische Kultur und die PCR zeigten eine hohe \u00dcbereinstimmung, sieben der Betriebe waren in beiden Tests positiv und lediglich in einem Betrieb lag nur in der PCR ein positives Ergebnis vor. Basierend auf diesen Ergebnissen konnte f\u00fcr die Stiefeltupfer ein negativer pr\u00e4diktiver Wert von 24,2 % (25%-CI: 20 %, 28 %) errechnet werden. Zwischen den Ergebnissen von Tier\u00e4rzten mit und ohne positiven Befund in der ersten Untersuchung lagen keine signifikanten Unterschiede vor, was auf einen zu vernachl\u00e4ssigenden Einfluss der durchf\u00fchrenden Person auf das Ergebnis der Sockentupfer hinweist. Die Ergebnisse der vorliegenden Untersuchung zeigen, dass negative Sockentupfer auch in kleinb\u00e4uerlichen Strukturen eine hohe Reproduzierbarkeit aufweisen. <\/p>

","schluesselwoerter":["Johne\u2018sche Krankheit","Mycobacterium avium subsp. paratuberculosis","bakteriologische Kultur","PCR","Umweltkotprobe"],"translatedTitle":"Reproduzierbarkeit negativer Sockentupferergebnisse zur Bestimmung des Paratuberkulosestatus in Rinderbetrieben in Tirol (\u00d6sterreich)","abstractE":"Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis or Johne\u2019s disease (JD) in cattle, leading to significant economic losses in the effected herds. Recently, boot swab samples have been used in cattle farms in order to establish the MAP-herd status. The aim of the present study was to evaluate the reproducibility of MAP-negative boot swab samples on cattle premises. Therefore, boot swab samples were collected from 158 farms with an average size of 16 cows, previously tested MAP-negative in boot swabs by the local veterinarians. Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). Boot swabs were tested for MAP by solid culture and PCR, leading to positive results in eight herds (5.1%). Culture and PCR showed a high correlation, as seven of the herds were positive both in culture and PCR, and one herd was positive in PCR only. Based on these results the reproducibility of a negative boot swab result was estimated as 24.2% (25% CI: 20%, 28%). No significant differences in the results between veterinarians with and without positive samples in the first survey were found, indicating no or little influence of the person collecting the boot swab samples. From the results of the study it is concluded that negative boot swab sample results have a high reproducibility for the detection of MAP in cattle herds in small structured agricultural systems.","date":{"year":2017,"date":"01\/2017","accepted":"2017-01-14"},"volume":"130","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"29-33","redirects":["reproducibility-of-negative-boot-swab-samples-for-paratuberculosis-in-cattle-herds-in-tyrol-austria\/150\/3130\/100034"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 01/2017 DO - 10.2376/0005-9366-16027 ET - 1/2 LA - English N2 - Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of paratuberculosis or Johne’s disease (JD) in cattle, leading to significant economic losses in the effected herds. Recently, boot swab samples have been used in cattle farms in order to establish the MAP-herd status. The aim of the present study was to evaluate the reproducibility of MAP-negative boot swab samples on cattle premises. Therefore, boot swab samples were collected from 158 farms with an average size of 16 cows, previously tested MAP-negative in boot swabs by the local veterinarians. Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). Boot swabs were tested for MAP by solid culture and PCR, leading to positive results in eight herds (5.1%). Culture and PCR showed a high correlation, as seven of the herds were positive both in culture and PCR, and one herd was positive in PCR only. Based on these results the reproducibility of a negative boot swab result was estimated as 24.2% (25% CI: 20%, 28%). No significant differences in the results between veterinarians with and without positive samples in the first survey were found, indicating no or little influence of the person collecting the boot swab samples. From the results of the study it is concluded that negative boot swab sample results have a high reproducibility for the detection of MAP in cattle herds in small structured agricultural systems. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 SP - 29 EP - 33 T1 - Reproducibility of negative boot swab samples for paratuberculosis in cattle herds in Tyrol (Austria) T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Reproducibility of negative boot swab samples for paratuberculosis in cattle herds in Tyrol (Austria) TT - Reproduzierbarkeit negativer Sockentupferergebnisse zur Bestimmung des Paratuberkulosestatus in Rinderbetrieben in Tirol (Österreich) VL - 130 SN - 0005-9366 ER -